FastQCFastQC Report
Thu 26 May 2016
SRR936418_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936418_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1509638
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT121990.8080745185269581No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120530.7984033258304308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89370.5919962269100274No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT78990.5232380213004707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60950.4037391745570792No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT53930.3572379603587085No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC51590.3417375556259183No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT37640.24933129664197642No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT32840.21753559462599645No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC29080.19262896138014543No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC28780.1906417300041467No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC25590.16951083637269332No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG23840.15791865334603394No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA21930.14526661358550857No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21600.14308065907190995No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA18940.12546054087138772No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17820.11804154373432571No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17570.1163855175876601No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16260.1077079405791322No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15710.10406468305646784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG114700.026.7219261
TACATGG116350.026.0562172
ACATGGG115900.024.8044173
GAGTACT87050.024.26105512-13
AACGCGG1000.001650265323.7589426
ATGGGAT26450.022.9969465
GTACTTT92900.022.68066614-15
CATGGGG37450.022.4583844
AGTACTT89400.022.18838112-13
CATGGGA74950.021.8094084
CAACGCG1100.002870397421.6004685
ACGCGGA1553.8991275E-521.4575547
GTACACG2056.8053123E-720.86221
ACTTTTT105000.020.31551216-17
TAAGGTG5150.020.3002455
AGAGTAC165500.019.63653210-11
TACTTTT101950.019.54891414-15
GTGTAAG5150.019.3768631
GTATCAA261400.018.8332651
GTGTAGC7000.018.3289321
Produced by FastQC (version 0.11.5)