FastQCFastQC Report
Thu 26 May 2016
SRR936418_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936418_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1509638
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT140160.9284344988666158No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113470.7516371474485937No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95850.6349204246316004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64470.4270560227021312No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT61590.4079786014925433No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44830.2969586086200798No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC42760.2832467121256884No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT36800.24376704878917993No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC35600.23581812328518492No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC33380.2211126111027942No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT30300.2007103689758737No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG27020.17898330593162068No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA26160.17328657598709094No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24930.16513892734549607No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC23520.15579893987830196No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22370.14818121960364009No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA21320.14122590978764446No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20370.13493301043031508No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19370.1283089058436526No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC17490.11585558922072708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT5500.025.043624
ATACGCT1951.5728801E-824.3555778
GTACATG112500.023.766411
TACGCTA2052.674642E-823.16759
TAAGGTG7400.023.1048855
TACATGG114150.022.8039252
ACATGGG112150.022.1933363
AGTACTT106900.021.74599512-13
GAGTACT103950.021.65499512-13
TACCGTG1856.5622553E-620.5376767
CATGGGA70700.020.4900114
GTACTTT110950.020.28875214-15
GGTAAGG6400.020.0389233
AGAGTAC184200.019.41375710-11
TACTTTT119350.019.35818714-15
CATGGGG39000.019.1203964
ACCGTGC3003.7252903E-918.997358
ATGGGAT26300.018.9612335
ACTTTTT126050.018.066116-17
TACCTGG10750.017.6748812
Produced by FastQC (version 0.11.5)