##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936418_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1509638 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.19313835502286 31.0 31.0 34.0 30.0 34.0 2 31.391388531555247 31.0 31.0 34.0 30.0 34.0 3 31.644094809484127 31.0 31.0 34.0 30.0 34.0 4 35.5132309865014 37.0 35.0 37.0 33.0 37.0 5 35.309949140124985 37.0 35.0 37.0 33.0 37.0 6 35.4437315435886 37.0 35.0 37.0 33.0 37.0 7 35.36598840251769 37.0 35.0 37.0 33.0 37.0 8 35.352516961019795 37.0 35.0 37.0 32.0 37.0 9 36.899464639867304 39.0 37.0 39.0 33.0 39.0 10-11 36.76339062742194 39.0 36.0 39.0 32.0 39.0 12-13 36.62650913662746 38.0 35.0 39.0 32.0 39.0 14-15 37.56396301629927 39.0 36.0 41.0 32.0 41.0 16-17 37.4944559556662 39.0 36.0 40.5 32.0 41.0 18-19 37.612216637366046 39.0 36.5 40.5 32.0 41.0 20-21 37.71357835454592 39.0 37.0 41.0 32.0 41.0 22-23 37.60702135213872 39.0 36.5 41.0 32.0 41.0 24-25 37.459757239815104 39.0 36.0 41.0 32.0 41.0 26-27 37.18586541939193 39.0 36.0 40.5 31.0 41.0 28-29 37.07314270043547 39.0 36.0 40.0 31.0 41.0 30-31 36.99562974699895 39.0 36.0 40.0 31.0 41.0 32-33 36.734580740548395 39.0 35.0 40.0 30.5 41.0 34-35 36.49717018252058 38.0 35.0 40.0 30.0 41.0 36-37 36.199282543232215 38.0 35.0 40.0 30.0 41.0 38-39 35.99541810685741 38.0 35.0 40.0 30.0 41.0 40-41 35.72270338981928 38.0 35.0 40.0 29.0 41.0 42-43 35.402560746351114 38.0 34.0 40.0 27.5 41.0 44-45 35.00721431230534 38.0 34.0 40.0 26.5 41.0 46-47 34.4614440680481 38.0 33.0 40.0 24.0 41.0 48-49 33.86361498584429 38.0 33.0 40.0 22.5 41.0 50-51 33.608772765391436 37.5 33.0 40.0 21.5 41.0 52-53 33.305768336515115 37.0 32.5 40.0 20.0 41.0 54-55 33.0132342985537 37.0 32.0 40.0 19.0 41.0 56-57 32.674663064920196 36.5 31.5 39.5 17.5 41.0 58-59 32.2650194947398 36.0 31.0 39.0 14.5 40.0 60-61 31.805200319546806 35.5 31.0 39.0 13.0 40.0 62-63 31.500185143723197 35.0 30.0 38.5 9.5 40.0 64-65 31.036049039571076 35.0 29.5 38.0 8.0 40.0 66-67 30.5616588215188 34.5 29.0 38.0 7.0 40.0 68-69 30.064788710936 34.0 28.5 37.0 4.0 39.0 70-71 29.53946807115348 34.0 28.0 36.5 2.0 39.0 72-73 29.07168241657934 34.0 27.5 36.0 2.0 39.0 74-75 28.68695972146965 33.0 27.0 35.5 2.0 38.0 76-77 27.39142695136185 31.5 26.0 34.5 2.0 36.0 78-79 28.188708153875297 33.0 27.0 35.0 2.0 37.0 80-81 27.99154101844283 33.0 26.0 35.0 2.0 36.5 82-83 27.610626521060016 33.0 26.0 35.0 2.0 36.0 84-85 27.093307799618188 32.5 25.5 35.0 2.0 36.0 86-87 26.67820795448975 32.0 25.0 35.0 2.0 35.0 88-89 26.18307667136095 32.0 24.0 34.0 2.0 35.0 90-91 25.608585303231635 32.0 22.0 34.0 2.0 35.0 92-93 25.047695871460576 31.0 20.0 34.0 2.0 35.0 94-95 24.375359854481673 31.0 16.5 34.0 2.0 35.0 96-97 23.52279652472977 31.0 4.5 34.0 2.0 35.0 98-99 22.49670682640474 30.5 2.0 34.0 2.0 35.0 100-101 19.640889405274642 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3960692903872278 1101 2 0.37403152850558996 1101 3 0.2669863118556073 1101 4 0.1271600409522975 1101 5 0.2963978469480253 1101 6 0.20901162477686341 1101 7 0.23349588029735457 1101 8 0.18206143799641694 1101 9 0.21368819993016075 1101 10-11 0.32302234042739997 1101 12-13 0.24531598287392598 1101 14-15 0.3425017429459345 1101 16-17 0.2931771992147745 1101 18-19 0.3305902251029167 1101 20-21 0.16909585727216836 1101 22-23 0.11114856115045768 1101 24-25 0.14822546161541794 1101 26-27 0.05498482647375624 1101 28-29 0.24640864528074502 1101 30-31 0.22853594000994093 1101 32-33 0.2591331150550289 1101 34-35 0.27174626278437586 1101 36-37 0.23807868975055868 1101 38-39 0.4338089005696659 1101 40-41 0.39660020581426636 1101 42-43 0.5061834370707246 1101 44-45 0.43337619625765456 1101 46-47 0.5046459813919952 1101 48-49 0.2612313546115175 1101 50-51 -0.020853287973622514 1101 52-53 0.0352817045806475 1101 54-55 -0.3565572260743082 1101 56-57 -0.14210595981612784 1101 58-59 0.005328841650964478 1101 60-61 0.11557815238530367 1101 62-63 -0.1369028989856993 1101 64-65 -0.037290525888998616 1101 66-67 -0.43076496035504874 1101 68-69 -0.47426106962208436 1101 70-71 -0.22350262022092338 1101 72-73 -0.1923456607928955 1101 74-75 -0.18576466659933288 1101 76-77 0.10570317941051854 1101 78-79 0.10738131001445694 1101 80-81 -0.07974926662150494 1101 82-83 0.1397355035487955 1101 84-85 0.1617557796446505 1101 86-87 0.13706334468868064 1101 88-89 0.12655801364842034 1101 90-91 0.06684449221452482 1101 92-93 0.06392334778566422 1101 94-95 -0.04903823428163179 1101 96-97 0.009306298151781078 1101 98-99 0.1130934729823494 1101 100-101 0.2751558950657049 1102 1 0.4552393692120944 1102 2 0.44415039649005905 1102 3 0.09297080481943709 1102 4 0.1747909069311504 1102 5 0.20316626654707193 1102 6 0.17881319900935466 1102 7 0.16543610312331225 1102 8 0.00852720333115542 1102 9 0.09475164200903663 1102 10-11 0.20842494049352212 1102 12-13 0.26345995125716115 1102 14-15 0.3994609571571104 1102 16-17 0.37947279544675183 1102 18-19 0.3414246727548047 1102 20-21 0.1642346917688826 1102 22-23 0.1940684878083232 1102 24-25 0.26376134267257356 1102 26-27 -0.21645735179644987 1102 28-29 -0.022838710788143146 1102 30-31 0.10101967976677173 1102 32-33 0.31785510728358446 1102 34-35 0.6152673143613541 1102 36-37 0.4797947497466666 1102 38-39 0.2810241107897511 1102 40-41 0.6338207729418599 1102 42-43 0.5029079539346029 1102 44-45 0.3001596894331797 1102 46-47 0.3632373065990535 1102 48-49 0.4318988730988238 1102 50-51 0.2658131396296426 1102 52-53 0.18376728512567553 1102 54-55 0.26615275888042333 1102 56-57 0.16750221841696344 1102 58-59 0.20281140968258882 1102 60-61 0.34875131324068676 1102 62-63 0.17154440625076361 1102 64-65 0.1497188633260329 1102 66-67 0.29367294968979607 1102 68-69 0.07742616750647713 1102 70-71 -0.024191059901298217 1102 72-73 -0.08842480084409843 1102 74-75 -0.4151496162371693 1102 76-77 -0.14434156633769035 1102 78-79 -0.08633726769672023 1102 80-81 -0.1976540772376829 1102 82-83 -0.04146181403403659 1102 84-85 0.29253635624669627 1102 86-87 0.19480187412792205 1102 88-89 0.15942718651736598 1102 90-91 0.21273623487032367 1102 92-93 0.4736923276109195 1102 94-95 0.1824564498605703 1102 96-97 0.10571757905033508 1102 98-99 0.13565476679346844 1102 100-101 -0.1812653635842203 1103 1 0.5059216569361027 1103 2 0.48037521343474765 1103 3 0.24986202629429854 1103 4 0.14756962440041832 1103 5 0.09105926171378798 1103 6 0.13790124239030632 1103 7 0.07169742199464224 1103 8 0.29462904494018716 1103 9 0.08179138605584058 1103 10-11 0.08212745184758319 1103 12-13 0.3322130493656985 1103 14-15 0.3506376136388454 1103 16-17 0.22095402901511108 1103 18-19 0.27090879636217124 1103 20-21 0.1791911814343763 1103 22-23 0.08494879508374709 1103 24-25 0.024523780263578487 1103 26-27 0.2008067403861773 1103 28-29 0.1376925806221223 1103 30-31 0.12096920531131872 1103 32-33 0.11976327325711367 1103 34-35 0.16944827015571917 1103 36-37 0.22158165808504293 1103 38-39 0.3093221985746908 1103 40-41 0.15507027827257502 1103 42-43 0.21340028865654403 1103 44-45 0.267928130751244 1103 46-47 0.14342304601535005 1103 48-49 0.29721011695124133 1103 50-51 0.19157399712598533 1103 52-53 0.18462898239724979 1103 54-55 0.30929725165739086 1103 56-57 0.22655584452069633 1103 58-59 0.3040842479027219 1103 60-61 0.23543369333459196 1103 62-63 -0.037761671214049386 1103 64-65 -0.1225687752723097 1103 66-67 -0.03617094201851501 1103 68-69 0.03996653584093579 1103 70-71 -0.10688995649224609 1103 72-73 -0.05166347928392412 1103 74-75 0.12303331601994927 1103 76-77 9.335077401217973E-4 1103 78-79 0.16678994368452038 1103 80-81 0.09287361111387327 1103 82-83 0.1855944680206143 1103 84-85 0.3393243986470118 1103 86-87 0.2675192858221429 1103 88-89 0.2258816343968597 1103 90-91 0.03235569619564771 1103 92-93 0.17545728860836007 1103 94-95 0.11081125655915969 1103 96-97 0.1314183961127 1103 98-99 -0.18673484247869965 1103 100-101 0.0564764075073505 1104 1 0.38101247301877805 1104 2 0.36358805050197773 1104 3 0.17216376890736385 1104 4 0.03283376123734172 1104 5 0.028660015179355014 1104 6 0.08702344156792918 1104 7 0.10895385014172376 1104 8 0.01972896262586943 1104 9 0.06643830944209839 1104 10-11 0.051617886846621275 1104 12-13 -0.06234384013188077 1104 14-15 0.020527111540673104 1104 16-17 -0.0884387293080593 1104 18-19 -0.05525682465987103 1104 20-21 -0.044525900434948085 1104 22-23 -0.003179035570774147 1104 24-25 -0.05844908800183646 1104 26-27 0.13526256210096932 1104 28-29 -0.10745805650608276 1104 30-31 -0.15767948976833424 1104 32-33 -0.06296892239184615 1104 34-35 0.03373937045154918 1104 36-37 0.028446602133378462 1104 38-39 -0.1595472799594475 1104 40-41 0.18176164883323054 1104 42-43 0.13056370449934462 1104 44-45 0.14423462667247833 1104 46-47 0.13158200447204393 1104 48-49 0.1654620941105378 1104 50-51 0.11657774813811983 1104 52-53 -0.032214389732267534 1104 54-55 0.1669718544739922 1104 56-57 0.329651413271705 1104 58-59 0.3589779510970459 1104 60-61 0.1499467671714676 1104 62-63 -0.012705390036263253 1104 64-65 -0.20906679515379523 1104 66-67 0.19749999840261268 1104 68-69 0.25845107575250736 1104 70-71 0.15241439311424188 1104 72-73 0.10530014277657074 1104 74-75 0.10179410957735868 1104 76-77 0.4325254436878687 1104 78-79 0.3535684337013869 1104 80-81 0.2561555691773947 1104 82-83 0.3643814626777022 1104 84-85 0.6832287080080981 1104 86-87 0.6323841550101896 1104 88-89 0.5587278960054007 1104 90-91 0.5219589142921315 1104 92-93 0.6597718195334963 1104 94-95 0.8466768190046317 1104 96-97 0.366306615951121 1104 98-99 0.18639536994855632 1104 100-101 -0.01987116559579505 1105 1 0.29975707646287475 1105 2 0.35726223596563855 1105 3 0.01570099907957001 1105 4 0.17499044326498847 1105 5 -0.054063567972072235 1105 6 0.03300969753041727 1105 7 0.07564315404665223 1105 8 0.012851769643411615 1105 9 0.08052028858350013 1105 10-11 -0.046100441231637035 1105 12-13 -0.10334650809210899 1105 14-15 -0.035876639773100294 1105 16-17 0.06888457896261713 1105 18-19 -0.07176503065874584 1105 20-21 0.056090155834759514 1105 22-23 -0.04754139307743799 1105 24-25 -0.1326613929577789 1105 26-27 -0.138911744633603 1105 28-29 -0.10714808803652431 1105 30-31 -0.09233726323426339 1105 32-33 -0.04348472803498993 1105 34-35 -0.25459598406377637 1105 36-37 -0.15445355147645756 1105 38-39 -0.07649351651858893 1105 40-41 -0.06904132634666382 1105 42-43 -0.19276332274618113 1105 44-45 -0.42000387280201323 1105 46-47 -0.3024453219084151 1105 48-49 -0.18598313185760418 1105 50-51 -0.35152573637962803 1105 52-53 -0.26653745546111196 1105 54-55 -0.017071479667819744 1105 56-57 -0.26409343271421903 1105 58-59 -0.07307395285638307 1105 60-61 -0.28686704154387144 1105 62-63 -0.45727180607635987 1105 64-65 -0.27156820998118647 1105 66-67 -0.30216108980177125 1105 68-69 -0.3691244760301764 1105 70-71 -0.4577114052526454 1105 72-73 -0.372737855444786 1105 74-75 -0.5426992205220849 1105 76-77 -0.40425618781022976 1105 78-79 -0.45429170050509526 1105 80-81 -0.6378791951527845 1105 82-83 -0.39414388294442304 1105 84-85 -0.101847207587344 1105 86-87 -0.3117354989634542 1105 88-89 -0.28321906817925324 1105 90-91 -0.26037320633201233 1105 92-93 -0.16185935517747652 1105 94-95 0.11415826455398914 1105 96-97 -0.008895259705759884 1105 98-99 0.009579620898513497 1105 100-101 0.11070010128107555 1106 1 0.3990457062004822 1106 2 0.45075494733590205 1106 3 0.26449400199502193 1106 4 0.028658081748957898 1106 5 -0.10777785368635762 1106 6 -0.0033984657348895553 1106 7 -0.0038320646122400603 1106 8 0.14115497664049315 1106 9 0.161978014531023 1106 10-11 0.007736293462237143 1106 12-13 -6.817850600384645E-4 1106 14-15 0.15720207742807446 1106 16-17 0.19103035155737302 1106 18-19 0.11782680607595353 1106 20-21 0.05918053484350594 1106 22-23 0.17154315502751416 1106 24-25 0.060936274680706504 1106 26-27 0.015356476940738162 1106 28-29 0.04504433178855294 1106 30-31 0.10956944230509436 1106 32-33 0.18479515534694002 1106 34-35 -0.08363388493841484 1106 36-37 0.2803102969200495 1106 38-39 0.2896172706534301 1106 40-41 0.31741348414897175 1106 42-43 0.22007632739958893 1106 44-45 0.28350050037582974 1106 46-47 0.28399199587584434 1106 48-49 0.22136963782168806 1106 50-51 0.20411274758539122 1106 52-53 0.4182263929353951 1106 54-55 0.08381481770827293 1106 56-57 0.15313688798548242 1106 58-59 -0.055143923701869824 1106 60-61 0.1905382062695402 1106 62-63 -0.24918434251950927 1106 64-65 0.11432100284680402 1106 66-67 0.15083016384254577 1106 68-69 0.19808835195816243 1106 70-71 0.18649267638000921 1106 72-73 0.4714137480537559 1106 74-75 0.3094465520726679 1106 76-77 0.2573881270585723 1106 78-79 0.34114270182726614 1106 80-81 0.27189339959940284 1106 82-83 0.38428177303225297 1106 84-85 0.48208282156717885 1106 86-87 0.5630516730482107 1106 88-89 0.359562272928617 1106 90-91 0.13555099191871633 1106 92-93 0.7038316069216464 1106 94-95 0.7923915007055946 1106 96-97 0.6659852067665426 1106 98-99 0.3699236442221263 1106 100-101 0.4964377106105218 1107 1 0.6267866841608765 1107 2 0.5899424728272216 1107 3 0.47140196132975376 1107 4 0.30491716421244064 1107 5 0.1948868391337797 1107 6 0.32483723268956055 1107 7 0.0766276100051968 1107 8 0.21289322042061087 1107 9 0.34830711315788676 1107 10-11 0.3848022234611861 1107 12-13 0.28753839198191145 1107 14-15 0.4893413469034371 1107 16-17 0.45676170701591445 1107 18-19 0.4738567309992945 1107 20-21 0.4336216140138873 1107 22-23 0.37140531306615543 1107 24-25 0.295107296901584 1107 26-27 0.3396582035529221 1107 28-29 0.38825457665630125 1107 30-31 0.37315348436010964 1107 32-33 0.34863222237212455 1107 34-35 0.4558906924721242 1107 36-37 0.5393699126242169 1107 38-39 0.6497422347767525 1107 40-41 0.7131407609960547 1107 42-43 0.45360270242035483 1107 44-45 0.38727888071390026 1107 46-47 0.7286879127325889 1107 48-49 0.5874561723614917 1107 50-51 0.5624827758335513 1107 52-53 0.355774165560355 1107 54-55 0.25359136267119453 1107 56-57 0.4745224074042085 1107 58-59 0.1801736415181523 1107 60-61 0.10477803617633796 1107 62-63 -0.035737387127436904 1107 64-65 0.06656650504472239 1107 66-67 0.05700397311458971 1107 68-69 -0.004401678102713191 1107 70-71 0.035087632497173615 1107 72-73 0.43710337327468096 1107 74-75 0.483079461935354 1107 76-77 0.22767053313721775 1107 78-79 0.39410800341792296 1107 80-81 0.2714113303838559 1107 82-83 0.22437018071212833 1107 84-85 0.3667712232046618 1107 86-87 0.56258093330894 1107 88-89 0.73360587630631 1107 90-91 0.6201145804925616 1107 92-93 0.7709396825723402 1107 94-95 0.9259832090395577 1107 96-97 0.9046321274148887 1107 98-99 1.1873727371087242 1107 100-101 1.122575378853245 1108 1 0.22704703403005766 1108 2 0.33138811998261275 1108 3 0.011990249433175393 1108 4 -0.016711112604880896 1108 5 -0.07994899937235544 1108 6 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-1.812404566520442 2316 94-95 -2.0502895685118183 2316 96-97 -2.134365029636207 2316 98-99 -2.0069217167151834 2316 100-101 -2.2980270255247035 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 7.0 4 49.0 5 101.0 6 227.0 7 563.0 8 1183.0 9 2063.0 10 3258.0 11 4942.0 12 6868.0 13 9553.0 14 11695.0 15 15161.0 16 19376.0 17 23908.0 18 20206.0 19 13922.0 20 12917.0 21 13361.0 22 14669.0 23 16364.0 24 18939.0 25 22842.0 26 27097.0 27 33062.0 28 39867.0 29 47783.0 30 57649.0 31 68722.0 32 83405.0 33 101986.0 34 127895.0 35 163495.0 36 206328.0 37 218386.0 38 97974.0 39 3811.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.98137705637818 19.95701676827942 15.423631139350139 20.637975035992266 2 16.00011394629817 22.666767363395284 37.952729510700024 23.380389179606528 3 20.88748216384225 26.472898976797428 29.992766171118472 22.646852688241847 4 13.162403127993608 17.540971442881126 37.11789467268557 32.17873075643969 5 12.804062960789276 39.257689591809424 33.056202877775995 14.8820445696253 6 30.132455595314905 37.2380663443819 16.803101140803292 15.826376919499907 7 27.482283832282974 34.58597359101984 20.192589216752626 17.73915335994457 8 27.27090865492257 32.5655554510419 19.183208159836994 20.98032773419853 9 27.079801719552272 16.951348687283012 20.535593036204133 35.433256556960586 10-11 25.49288404150672 26.739410519761396 26.01834218205063 21.74936325668125 12-13 27.795616888977502 24.57202417404731 26.775370610985235 20.85698832598995 14-15 23.55649073254034 25.552102922755605 27.42891171851246 23.462494626191592 16-17 21.20077793484266 28.80607801340454 27.69584496415697 22.297299087595835 18-19 21.08704205908966 27.124648425649063 31.00080946558049 20.787500049680784 20-21 22.85008061535282 24.97542457198348 30.839413157326454 21.33508165533724 22-23 21.944748790088063 25.328660174057738 31.1257712236373 21.600819812216898 24-25 22.473765233784523 25.55231784043592 31.113253640939085 20.860663284840474 26-27 21.878894767783656 25.18498646198177 32.00619353983986 20.929925230394712 28-29 20.992184881408654 26.771020602290086 31.3075386284659 20.929255887835364 30-31 21.516714603103527 25.556755990508982 31.105105992297492 21.82142341409 32-33 21.43386617363868 25.876119431657617 31.144660479085946 21.545353915617756 34-35 22.51431065896101 25.459575597010254 30.940666449615183 21.08544729441355 36-37 22.377797017618587 25.113689132014528 31.38204251592642 21.12647133444047 38-39 21.73862210675672 25.821720173975482 31.30806855683283 21.13158916243497 40-41 21.773265875611717 26.06980383714094 30.784800483575815 21.372129803671534 42-43 21.443335166181956 26.301598553153717 29.901291164565514 22.353775116098813 44-45 22.056987736199954 25.625569064235656 29.8763816109573 22.441061588607088 46-47 22.917320805660772 26.570430400273448 29.21169461413417 21.30055417993161 48-49 22.713559649899175 26.605523124090762 28.790109095148882 21.89080813086118 50-51 21.8337647850602 26.995751282431748 29.366229650669833 21.804254281838222 52-53 22.698621466516386 27.422220882657555 27.98746564927466 21.891692001551394 54-55 21.840427110577597 26.8164057841976 28.463383707896433 22.879783397328374 56-57 22.849799281919474 26.776288769061594 28.002755733382795 22.371156215636137 58-59 23.599320095599964 26.279590149600164 27.845841083607574 22.275248671192298 60-61 22.468357470081486 26.85758783114401 29.161473371187668 21.51258132758684 62-63 22.204616229472034 26.90785625472397 28.97487855950005 21.912648956303943 64-65 21.86713572799743 27.654842412287024 28.416404558772623 22.061617300942927 66-67 22.336333260907583 26.990007896173267 27.795722287876462 22.877936555042687 68-69 22.210718345349477 26.96918329981532 28.20024164765546 22.619856707179746 70-71 22.829538009871353 26.991542910439108 27.935620927371836 22.2432981523177 72-73 22.650205761316872 26.57747306886587 28.536519528694804 22.235801641122453 74-75 21.93939871677846 28.316059876606182 27.577637817807975 22.166903588807383 76-77 22.238602301896165 27.061654384366978 27.819955286909 22.879788026827853 78-79 22.369802656116576 27.196410459908165 27.965872075267455 22.4679148087078 80-81 22.746636102848132 27.307259127738654 27.657959070100237 22.288145699312977 82-83 22.494329894004544 27.04671200101494 28.171988301710922 22.2869698032696 84-85 22.038164998315697 27.60611040099051 27.809618194338682 22.54610640635511 86-87 22.17245574594674 27.715569682433422 27.28694937278672 22.825025198833117 88-89 21.969799116852258 27.779167347187578 26.872199994633135 23.378833541327026 90-91 22.26776924774085 27.10860017213198 27.241873257064338 23.381757323062835 92-93 22.7146715315412 27.623928770393686 27.37439197348696 22.28700772457815 94-95 22.208991392305443 27.303420322267684 27.452540424233774 23.0350478611931 96-97 21.493569686735462 27.309172429646818 28.064294520470806 23.132963363146917 98-99 21.812037075988023 27.866481129941185 26.813512735554852 23.507969058515947 100-101 21.59334147309341 27.421639351212978 27.175823455096282 23.809195720597327 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1461.0 1 1084.5 2 1245.5 3 1561.5 4 1053.0 5 848.5 6 1353.0 7 2576.0 8 3849.5 9 4367.0 10 3784.0 11 2953.5 12 2724.5 13 2751.0 14 2895.5 15 3060.0 16 3261.0 17 3457.0 18 3601.5 19 3863.0 20 4231.0 21 4597.5 22 5110.5 23 5851.5 24 6566.0 25 7454.0 26 8935.5 27 10638.0 28 12310.0 29 14512.5 30 17356.0 31 20293.5 32 23731.0 33 26385.0 34 28234.0 35 29795.0 36 32606.5 37 35887.5 38 37087.5 39 37764.0 40 38209.5 41 40068.0 42 41857.5 43 43342.5 44 45415.5 45 47553.5 46 49821.5 47 60074.0 48 81588.5 49 79749.0 50 59210.5 51 49885.5 52 46177.0 53 45960.0 54 46113.0 55 43291.5 56 39308.0 57 35933.5 58 34097.0 59 31350.0 60 26487.0 61 22378.5 62 19201.0 63 14857.0 64 10815.0 65 8385.0 66 6232.5 67 4848.5 68 3713.5 69 2675.0 70 2105.0 71 1721.5 72 1446.5 73 1301.0 74 1106.0 75 909.0 76 679.0 77 427.0 78 290.5 79 223.5 80 153.5 81 110.5 82 84.5 83 52.0 84 26.0 85 11.5 86 6.5 87 7.0 88 5.5 89 2.5 90 2.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0311743610057509 2 0.010267362109326873 3 0.004239426935463999 4 0.011393459889059496 5 0.0 6 0.0 7 0.0 8 0.0 9 0.003908221706130873 10-11 1.9872313759987495E-4 12-13 1.6560261466656243E-4 14-15 6.624104586662498E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0015897851007989996 24-25 0.0 26-27 3.312052293331249E-5 28-29 0.0 30-31 0.0 32-33 0.0036432575226643736 34-35 7.617720274661873E-4 36-37 0.002583400788798374 38-39 0.0 40-41 0.003345172816264561 42-43 0.009803674788260497 44-45 0.0021528339906653118 46-47 0.0013248209173324994 48-49 0.008611335962661247 50-51 3.974462751997499E-4 52-53 0.0017553877154655622 54-55 0.009671192696527248 56-57 0.0038419806602642487 58-59 9.273746421327497E-4 60-61 0.005266163146396686 62-63 0.0033782933391978743 64-65 0.015930971530923307 66-67 0.003047088109864749 68-69 1.3248209173324997E-4 70-71 0.0011260977797326248 72-73 3.312052293331249E-5 74-75 0.0 76-77 8.611335962661248E-4 78-79 0.009339987467194121 80-81 0.015102958457590495 82-83 0.012353955054125558 84-85 0.007518358705861936 86-87 0.009505590081860684 88-89 0.02500599481465093 90-91 0.021925786181852865 92-93 0.002715882880531624 94-95 0.00771708184346181 96-97 0.009737433742393872 98-99 0.009505590081860684 100-101 0.011592183026659372 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1509638.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.72845868684458 #Duplication Level Percentage of deduplicated Percentage of total 1 76.37025850266937 38.74145503356304 2 13.251667320083252 13.444733163573034 3 4.321838810948045 6.5772066471713835 4 1.9540233742115447 3.9649837604727596 5 1.0482498829829583 2.658805044119534 6 0.655551701143855 1.9953076433140042 7 0.41900154466919076 1.4878711783932577 8 0.2962598985703335 1.2023046420155146 9 0.21867358600935694 0.9983676576401836 >10 1.3006702177214668 12.25352989792908 >50 0.10364958159572273 3.6440643899848837 >100 0.05253769709383224 4.952038178037432 >500 0.0036775983522613965 1.257207075044316 >1k 0.0032835699573762474 3.653689351539836 >5k 3.940283948851497E-4 1.4785458796072084 >10k+ 2.626855965900997E-4 1.6898904575944136 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14016 0.9284344988666158 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11347 0.7516371474485937 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9585 0.6349204246316004 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6447 0.4270560227021312 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6159 0.4079786014925433 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4483 0.2969586086200798 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4276 0.2832467121256884 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3680 0.24376704878917993 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3560 0.23581812328518492 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3338 0.2211126111027942 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3030 0.2007103689758737 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2702 0.17898330593162068 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2616 0.17328657598709094 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2493 0.16513892734549607 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2352 0.15579893987830196 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2237 0.14818121960364009 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2132 0.14122590978764446 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2037 0.13493301043031508 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1937 0.1283089058436526 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1749 0.11585558922072708 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 9.936156879993747E-5 0.0 24-25 0.0 0.0 0.0 2.6496418346649993E-4 0.0 26-27 0.0 0.0 0.0 4.968078439996873E-4 0.0 28-29 0.0 0.0 0.0 0.0011923388255992496 0.0 30-31 0.0 0.0 0.0 0.003378293339197874 0.0 32-33 0.0 0.0 0.0 0.006822827724262372 0.0 34-35 0.0 0.0 0.0 0.012353955054125558 0.0 36-37 0.0 0.0 0.0 0.018249408136255182 0.0 38-39 0.0 0.0 0.0 0.025999610502650303 0.0 40-41 0.0 0.0 0.0 0.03689626254771011 0.0 42-43 0.0 0.0 0.0 0.047958517207436484 0.0 44-45 0.0 0.0 0.0 0.0639226092612931 0.0 46-47 0.0 0.0 0.0 0.08488790027807991 0.0 48-49 0.0 0.0 0.0 0.10678056593699947 0.0 50-51 0.0 0.0 0.0 0.13665527762284735 0.0 52-53 0.0 0.0 0.0 0.17298849128069113 0.0 54-55 0.0 0.0 0.0 0.21134205683746699 0.0 56-57 0.0 0.0 0.0 0.26095660019156913 0.0 58-59 0.0 0.0 0.0 0.3206729030403315 0.0 60-61 0.0 0.0 0.0 0.39373677663121887 0.0 62-63 0.0 0.0 0.0 0.4655420703506403 0.0 64-65 0.0 0.0 0.0 0.5404275727028598 0.0 66-67 0.0 0.0 0.0 0.6408489982366634 0.0 68-69 0.0 0.0 0.0 0.7600166397507218 0.0 70-71 0.0 0.0 0.0 0.8960426274378361 0.0 72-73 0.0 0.0 0.0 1.0237884843916225 0.0 74-75 0.0 0.0 0.0 1.1808791246643235 0.0 76-77 0.0 0.0 0.0 1.3299214778642297 0.0 78-79 0.0 0.0 0.0 1.5005252914937222 0.0 80-81 0.0 0.0 0.0 1.6907695752226692 0.0 82-83 0.0 0.0 0.0 1.9165521800590606 0.0 84-85 0.0 0.0 0.0 2.1419042180973187 0.0 86-87 0.0 0.0 0.0 2.382094250409701 0.0 88-89 0.0 0.0 0.0 2.6559016134993954 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGGT 550 0.0 25.04362 4 ATACGCT 195 1.5728801E-8 24.355577 8 GTACATG 11250 0.0 23.76641 1 TACGCTA 205 2.674642E-8 23.1675 9 TAAGGTG 740 0.0 23.104885 5 TACATGG 11415 0.0 22.803925 2 ACATGGG 11215 0.0 22.193336 3 AGTACTT 10690 0.0 21.745995 12-13 GAGTACT 10395 0.0 21.654995 12-13 TACCGTG 185 6.5622553E-6 20.537676 7 CATGGGA 7070 0.0 20.490011 4 GTACTTT 11095 0.0 20.288752 14-15 GGTAAGG 640 0.0 20.038923 3 AGAGTAC 18420 0.0 19.413757 10-11 TACTTTT 11935 0.0 19.358187 14-15 CATGGGG 3900 0.0 19.120396 4 ACCGTGC 300 3.7252903E-9 18.99735 8 ATGGGAT 2630 0.0 18.961233 5 ACTTTTT 12605 0.0 18.0661 16-17 TACCTGG 1075 0.0 17.674881 2 >>END_MODULE