FastQCFastQC Report
Thu 26 May 2016
SRR936417_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936417_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1528604
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT124360.8135527579412327No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121610.7955624870797146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91340.5975386692694772No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT82230.5379418083427755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59120.38675811393925436No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT55610.36379598640328037No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC50450.33003969635039554No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT36680.23995750370926677No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT33740.22072426867913467No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC29620.19377157197024214No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC28910.189126811129632No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC26300.1720524086028821No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG24770.16204327608720112No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA22720.148632346899524No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20750.13574477104600016No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA20720.13554851354569267No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18820.1231188718595529No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17650.11546482934756157No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17510.11454896101279338No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT15610.1021193193266536No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15400.10074551682450132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG756.899447E-638.01952
TCGAGTA650.005831900529.2438539
GTACATG116600.025.9622961
TACATGG119000.025.0002162
ACATGGG119350.023.6925033
GAGTACT92700.023.55634912-13
AGTACTT96350.021.77615412-13
GTACTTT99650.021.74580614-15
GTGTAGC7750.021.4619181
CATGGGA76950.021.4307424
ATGGGAT28700.021.1934575
TAAGGTG5750.020.6607425
CATGGGG40300.020.5192094
CGTACAC1656.299552E-520.1618543
ACTTTTT112200.019.60832816-17
TACTTTT107100.019.52320714-15
AGAGTAC171000.019.4266310-11
TACCTGG12000.019.4057872
GTACCTG12300.019.3182181
AGTACAT90150.019.2417052
Produced by FastQC (version 0.11.5)