FastQCFastQC Report
Thu 26 May 2016
SRR936417_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936417_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1528604
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT144490.9452415406475451No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115850.7578810470206804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98850.6466684635131139No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66620.4358224890161219No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT64230.42018730815829347No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT45150.29536753796274245No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC43180.2824799621092186No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT38940.2547422353990962No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC37130.2429013662138788No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC34490.22563070618682146No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT31770.20783669282561082No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG27310.17865974444656693No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA25490.16675345609458042No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25460.16655719859427293No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC23730.15523968274320882No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA23170.15157620940413605No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23080.15098743690321365No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20840.13633354354692256No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19700.1288757585352387No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC18560.12141797352355484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT103900.022.46916612-13
GTACATG118200.021.7097171
AGTACTT106450.021.59626412-13
TACATGG117950.021.2229542
GTACTTT111450.020.9044114-15
ACATGGG117000.019.9330883
GTGTAAG5800.019.6635131
TACCTGG10650.019.6243952
TACTTTT120300.019.05068814-15
GGACCGT1507.7261013E-418.998746
CCGAACC1250.006034351418.9987354
ACTTTTT125600.018.7573316-17
AGAGTAC192550.018.53806710-11
ATGGGAT25900.018.5219355
CATGGGA74850.017.8311464
TAAGGTG7000.017.6416845
CATGGGG40500.017.3568734
ATACCGT1650.001464763517.271586
TAGGACA7500.017.0988664
AGTACAT95250.016.4068072
Produced by FastQC (version 0.11.5)