##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936417_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1528604 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.530973358698525 31.0 31.0 34.0 30.0 34.0 2 31.657046560129373 31.0 31.0 34.0 30.0 34.0 3 31.741284204411347 31.0 31.0 34.0 30.0 34.0 4 35.55992722771889 37.0 35.0 37.0 33.0 37.0 5 35.32574885320201 37.0 35.0 37.0 33.0 37.0 6 35.44927201551219 37.0 35.0 37.0 33.0 37.0 7 35.37715719702421 37.0 35.0 37.0 33.0 37.0 8 35.363406088169334 37.0 35.0 37.0 33.0 37.0 9 36.904770627317475 39.0 37.0 39.0 33.0 39.0 10-11 36.74721804993314 39.0 36.0 39.0 32.0 39.0 12-13 36.63513571860338 38.0 35.0 39.0 32.0 39.0 14-15 37.54412097573996 39.0 36.0 41.0 32.0 41.0 16-17 37.481868750834096 39.0 36.0 40.0 32.0 41.0 18-19 37.70705231701605 39.0 37.0 41.0 32.0 41.0 20-21 37.75477036564081 39.0 37.0 41.0 32.0 41.0 22-23 37.641476144246646 39.0 36.5 41.0 32.0 41.0 24-25 37.46862300504251 39.0 36.0 41.0 32.0 41.0 26-27 37.18292082187408 39.0 36.0 40.5 31.0 41.0 28-29 37.06611032026608 39.0 36.0 40.0 31.0 41.0 30-31 36.96366619477641 39.0 36.0 40.0 31.0 41.0 32-33 36.72918100436738 39.0 35.0 40.0 30.5 41.0 34-35 36.47300576212021 38.0 35.0 40.0 30.0 41.0 36-37 36.14101657460009 38.0 35.0 40.0 30.0 41.0 38-39 35.9203371180502 38.0 35.0 40.0 30.0 41.0 40-41 35.664449065945135 38.0 35.0 40.0 28.5 41.0 42-43 35.3282557156726 38.0 34.0 40.0 27.0 41.0 44-45 34.95805715541762 38.0 34.0 40.0 26.0 41.0 46-47 34.44347489604894 38.0 33.0 40.0 24.0 41.0 48-49 33.94474141111759 38.0 33.0 40.0 23.0 41.0 50-51 33.54795944535014 37.5 33.0 40.0 21.5 41.0 52-53 33.236735282650045 37.0 32.5 40.0 20.0 41.0 54-55 32.936560090121446 37.0 32.0 40.0 18.5 41.0 56-57 32.545442442908694 36.0 31.0 39.0 16.5 40.5 58-59 32.15554551734786 36.0 31.0 39.0 13.5 40.0 60-61 31.697856344743307 35.0 30.5 39.0 11.0 40.0 62-63 31.28430711943708 35.0 30.0 38.5 8.0 40.0 64-65 30.831398779540024 35.0 29.0 38.0 7.5 40.0 66-67 30.300711302600284 34.0 29.0 37.5 6.5 39.5 68-69 29.959817258099548 34.0 28.5 37.0 2.0 39.0 70-71 29.46172128294836 34.0 28.0 36.5 2.0 39.0 72-73 28.965461950904224 34.0 27.5 36.0 2.0 39.0 74-75 28.40907847944922 33.0 26.0 35.5 2.0 38.0 76-77 27.147300739759938 31.5 25.5 34.0 2.0 36.0 78-79 27.912442987196158 33.0 26.0 35.0 2.0 37.0 80-81 27.660051916650747 33.0 26.0 35.0 2.0 36.0 82-83 27.259022611480802 33.0 25.5 35.0 2.0 36.0 84-85 26.765004540090175 32.0 25.0 35.0 2.0 35.5 86-87 26.345198952769977 32.0 24.5 34.0 2.0 35.0 88-89 25.843931129317994 32.0 23.5 34.0 2.0 35.0 90-91 25.21405151366868 31.0 20.0 34.0 2.0 35.0 92-93 24.595316380174328 31.0 18.5 34.0 2.0 35.0 94-95 23.836758244777588 31.0 10.0 34.0 2.0 35.0 96-97 22.854518567267913 30.0 2.0 34.0 2.0 35.0 98-99 21.70227933460857 30.0 2.0 34.0 2.0 35.0 100-101 18.48627440462016 24.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24637769732139247 1101 2 0.3108202827467572 1101 3 0.3502026931107416 1101 4 0.19569749300431738 1101 5 0.21436947096049153 1101 6 0.21071836467169902 1101 7 0.13794916412904712 1101 8 0.2012149228275817 1101 9 0.3341139839818652 1101 10-11 0.29755307088584004 1101 12-13 0.2465194428919233 1101 14-15 0.4630487694457699 1101 16-17 0.42456228502155113 1101 18-19 0.33708182777133544 1101 20-21 0.26317199408801883 1101 22-23 0.27311853998919133 1101 24-25 0.22590371337837922 1101 26-27 0.4256541635880282 1101 28-29 0.3281083676606684 1101 30-31 0.4185656380700493 1101 32-33 0.46280973328367025 1101 34-35 0.46786605722166286 1101 36-37 0.39550201717602107 1101 38-39 0.41107041062117844 1101 40-41 0.5606464864995857 1101 42-43 0.6591250019668138 1101 44-45 0.5971236218697697 1101 46-47 0.4842922495221629 1101 48-49 0.4876605259057243 1101 50-51 0.5193187700565502 1101 52-53 0.5079058449224476 1101 54-55 0.5933243219092645 1101 56-57 0.4778912755240583 1101 58-59 0.5853306102290716 1101 60-61 0.40817958257948206 1101 62-63 0.6414785257307507 1101 64-65 0.6709151731341088 1101 66-67 0.47792362975507174 1101 68-69 0.520590411051824 1101 70-71 0.4479064421791499 1101 72-73 0.47697752847343367 1101 74-75 0.565437554635718 1101 76-77 0.33471081083810716 1101 78-79 0.17977476029853534 1101 80-81 0.44394820547017133 1101 82-83 0.5928123613968985 1101 84-85 0.4678209763211001 1101 86-87 0.5857793213834874 1101 88-89 0.35437460837656687 1101 90-91 0.42923063118531246 1101 92-93 0.4189889230809918 1101 94-95 0.6261067424979707 1101 96-97 0.7779089355002959 1101 98-99 1.0045031874848256 1101 100-101 1.4454732697870227 1102 1 0.23558473643736733 1102 2 0.2624170231136773 1102 3 0.23604716736418396 1102 4 0.21214283945585777 1102 5 0.14813862902787633 1102 6 0.2692498965511163 1102 7 0.2211093091451133 1102 8 0.24367048522696422 1102 9 0.31367164318903207 1102 10-11 0.25776053603557614 1102 12-13 0.16863945780627176 1102 14-15 0.407454528308115 1102 16-17 0.4502805127464171 1102 18-19 0.33063181583859347 1102 20-21 0.14440954304550502 1102 22-23 0.12694496256531096 1102 24-25 0.3797208936172538 1102 26-27 0.15620933052932173 1102 28-29 0.19010618748193764 1102 30-31 0.3737778098483844 1102 32-33 0.4568265859892975 1102 34-35 0.37991936271826177 1102 36-37 0.5111872763444509 1102 38-39 0.6348117277302094 1102 40-41 0.6103558593057201 1102 42-43 0.5708535595861335 1102 44-45 0.675716564648944 1102 46-47 0.7507152814265865 1102 48-49 0.6784695279422053 1102 50-51 0.6464039141388938 1102 52-53 0.4964216078552326 1102 54-55 0.5807628144713988 1102 56-57 0.4999977117990966 1102 58-59 0.0727175102921862 1102 60-61 0.21525560460156967 1102 62-63 0.21066833991545764 1102 64-65 0.41215584296300634 1102 66-67 0.45396287044009753 1102 68-69 0.27592991818203316 1102 70-71 0.17286445601353861 1102 72-73 0.23858014342183864 1102 74-75 0.1148732972859996 1102 76-77 -0.05527783881210979 1102 78-79 0.14781067500821976 1102 80-81 0.5491060626912514 1102 82-83 0.25540089950602507 1102 84-85 0.4644972166142729 1102 86-87 0.5437483684162245 1102 88-89 0.3636321385075405 1102 90-91 0.6001461353326505 1102 92-93 0.6479176602295524 1102 94-95 1.0008930134491152 1102 96-97 1.0529707040658387 1102 98-99 1.3151701107424039 1102 100-101 1.4069745038922576 1103 1 0.13024381984861222 1103 2 0.15928055812906905 1103 3 0.19396837852718107 1103 4 0.12630710237911558 1103 5 0.08631656870128523 1103 6 0.1764002499231836 1103 7 0.05407206969243106 1103 8 0.08166398920636908 1103 9 0.1565178662721891 1103 10-11 0.09975445208527844 1103 12-13 0.22942060490540683 1103 14-15 0.20350478470050604 1103 16-17 0.31742316625504685 1103 18-19 0.15247661472712082 1103 20-21 0.2964105738437084 1103 22-23 0.20820046357929556 1103 24-25 0.15528465807239655 1103 26-27 0.2856182337376225 1103 28-29 0.2558444718004864 1103 30-31 0.1992191981393603 1103 32-33 0.3271310387138797 1103 34-35 0.33032371447939823 1103 36-37 0.34452709366409806 1103 38-39 0.47828975044013333 1103 40-41 0.4223900237927225 1103 42-43 0.3880024999679108 1103 44-45 0.3084164280806263 1103 46-47 0.28881980162611853 1103 48-49 0.38692263347079603 1103 50-51 0.4854429998158949 1103 52-53 0.30193220777232455 1103 54-55 0.19754926370769965 1103 56-57 0.25704912472714625 1103 58-59 0.45423142669046257 1103 60-61 0.2992483852793306 1103 62-63 0.20418834773358796 1103 64-65 0.08113563284450365 1103 66-67 0.3554298282533104 1103 68-69 0.3372378243296019 1103 70-71 0.33896862389919136 1103 72-73 0.3009029749796994 1103 74-75 0.22998718928374373 1103 76-77 0.001114635116042706 1103 78-79 -0.10273740338721993 1103 80-81 0.16825880383018443 1103 82-83 0.47302023493996614 1103 84-85 0.23054523836561103 1103 86-87 0.11955483107282205 1103 88-89 0.10716708543014519 1103 90-91 0.27847375860827484 1103 92-93 0.4572528265080109 1103 94-95 0.5365182647684215 1103 96-97 0.617554164485302 1103 98-99 0.8830132526352621 1103 100-101 1.1841018946158215 1104 1 0.11496503531587621 1104 2 0.24662411466780298 1104 3 0.11597449792811076 1104 4 0.11060986249434279 1104 5 0.12078170326425663 1104 6 0.10275023008019701 1104 7 0.05512431119844763 1104 8 0.03943348759827359 1104 9 0.1387324183778631 1104 10-11 0.1729849211433674 1104 12-13 0.04195276368432843 1104 14-15 0.05865804514686346 1104 16-17 0.15333342714333753 1104 18-19 0.22998678347385493 1104 20-21 0.14304934765998922 1104 22-23 0.06608823742186587 1104 24-25 0.10174569008281509 1104 26-27 0.24332216264841122 1104 28-29 0.13729484994390617 1104 30-31 0.22024634484348837 1104 32-33 0.19619316665793463 1104 34-35 0.08759926654625616 1104 36-37 0.1262372145873698 1104 38-39 0.24507710250975379 1104 40-41 0.19847650540010164 1104 42-43 0.1329273595334186 1104 44-45 0.24370905262508558 1104 46-47 0.3150887510496716 1104 48-49 0.2913886819377538 1104 50-51 0.04272271452762055 1104 52-53 0.30157134089057536 1104 54-55 0.39703322093699 1104 56-57 0.20728564433039054 1104 58-59 -0.09768238840798205 1104 60-61 0.06195837975834806 1104 62-63 0.0690061428212907 1104 64-65 0.12753550840333006 1104 66-67 -0.022656945052180077 1104 68-69 0.3208917752116456 1104 70-71 0.028663793102882806 1104 72-73 -0.01997747761559765 1104 74-75 0.25492424884149045 1104 76-77 0.12112071320350282 1104 78-79 0.07191277795386597 1104 80-81 0.09909536399574037 1104 82-83 0.08339871012246292 1104 84-85 0.16358800030864273 1104 86-87 0.08048778307032833 1104 88-89 0.28414224476867744 1104 90-91 0.36712886125972233 1104 92-93 0.413824703804508 1104 94-95 0.5101012754185348 1104 96-97 0.4251365316468565 1104 98-99 0.4857745571893872 1104 100-101 1.1954826595659753 1105 1 0.14597788849373572 1105 2 0.23836743092598667 1105 3 0.12216634903518298 1105 4 0.11817904067375196 1105 5 -0.031385643133006624 1105 6 0.18753938456415398 1105 7 0.17720948446936546 1105 8 0.16477786072256606 1105 9 0.07238151461243092 1105 10-11 0.16368027115086647 1105 12-13 -0.11439801104973668 1105 14-15 0.08045011871723062 1105 16-17 0.27493043176298926 1105 18-19 0.10613057205299725 1105 20-21 0.2596078430352051 1105 22-23 0.21942464103990034 1105 24-25 0.2062888843236479 1105 26-27 0.290561040393186 1105 28-29 0.17211776763414122 1105 30-31 0.2368194306880511 1105 32-33 0.3182146513721804 1105 34-35 0.2634761256317688 1105 36-37 0.14630473438293023 1105 38-39 0.3299023196050186 1105 40-41 0.4254822069320241 1105 42-43 0.2664766384300705 1105 44-45 0.2879165204970988 1105 46-47 0.32086455872809694 1105 48-49 0.434177929721308 1105 50-51 0.3894647222754628 1105 52-53 0.4853871026338368 1105 54-55 0.41613978793851203 1105 56-57 0.2845104179547917 1105 58-59 0.35684183160571337 1105 60-61 0.2570791084480746 1105 62-63 0.23529845311458786 1105 64-65 0.49040412039974157 1105 66-67 0.7572825491647315 1105 68-69 0.6248906556164435 1105 70-71 0.33631541901383244 1105 72-73 0.28551006076709484 1105 74-75 0.200132735399567 1105 76-77 0.23478526673348554 1105 78-79 0.5196757546213568 1105 80-81 0.49341371612629814 1105 82-83 0.6465562531138929 1105 84-85 0.45369235359519067 1105 86-87 0.5689012630759258 1105 88-89 0.7643309680232058 1105 90-91 0.8294930598872909 1105 92-93 1.000147015543618 1105 94-95 0.8731316728913718 1105 96-97 1.1202144153075473 1105 98-99 1.24133125969038 1105 100-101 1.1108026854648863 1106 1 -0.03334752347032577 1106 2 0.05753943329682443 1106 3 0.03163315472312789 1106 4 -0.011405900996130924 1106 5 -0.008617755122422466 1106 6 -0.08842553996772295 1106 7 -0.0047475163999166625 1106 8 -0.14956938998942348 1106 9 -0.11478637628773924 1106 10-11 -0.06600532449466812 1106 12-13 -8.695456857523709E-4 1106 14-15 0.04663058930037067 1106 16-17 -0.11336481118426889 1106 18-19 -0.14445327948548936 1106 20-21 -0.14318504678997357 1106 22-23 -0.11008606574295499 1106 24-25 -0.3860532263620087 1106 26-27 -0.14730026558355291 1106 28-29 -0.22106896269060172 1106 30-31 -0.24465194742862195 1106 32-33 -0.33224498228999977 1106 34-35 -0.2658387427767437 1106 36-37 -0.49368943140166976 1106 38-39 -0.15433824683675823 1106 40-41 -0.17709024862249834 1106 42-43 -0.27675107932186904 1106 44-45 -0.18315113748165146 1106 46-47 -0.41155290139989376 1106 48-49 -0.43673283051892753 1106 50-51 -0.1396282670684954 1106 52-53 -0.2732927547718873 1106 54-55 -0.4068284104275648 1106 56-57 -0.342393399018718 1106 58-59 -0.16515687564773884 1106 60-61 -0.3522388146675617 1106 62-63 -0.30483895366610625 1106 64-65 -0.24368792466819755 1106 66-67 -0.24485178983689693 1106 68-69 -0.18841857947182206 1106 70-71 -0.35194982725988666 1106 72-73 -0.4590219962857489 1106 74-75 -0.5413072906892857 1106 76-77 -0.727433544552003 1106 78-79 -0.4297313466783059 1106 80-81 -0.10567971009581711 1106 82-83 -0.2713885688307336 1106 84-85 -0.38288821138115026 1106 86-87 -0.14315784688286826 1106 88-89 -0.25128175015357357 1106 90-91 -0.08149869137148613 1106 92-93 -0.18406403582259045 1106 94-95 -0.004965733049964172 1106 96-97 -0.07242528852345842 1106 98-99 0.6548202684207176 1106 100-101 0.5524431595086483 1107 1 -0.028268253730729498 1107 2 0.13862245979492016 1107 3 -0.03537614797248523 1107 4 0.031135221698811222 1107 5 -0.06349680561445581 1107 6 -0.007984482468451404 1107 7 -0.09323557264246318 1107 8 -0.06446510274549411 1107 9 0.27266085671910645 1107 10-11 0.04037365694436801 1107 12-13 0.08769910108561874 1107 14-15 0.21341664934307403 1107 16-17 0.029730388616528103 1107 18-19 -0.08093984928050446 1107 20-21 0.12345371167504737 1107 22-23 0.039478934823584666 1107 24-25 0.12835698011850383 1107 26-27 0.20091074521798902 1107 28-29 0.025019210924817514 1107 30-31 0.13681918790251046 1107 32-33 0.16588077130893453 1107 34-35 0.10016417708011005 1107 36-37 0.007828012487173908 1107 38-39 0.14953850200355134 1107 40-41 0.18658036755408602 1107 42-43 0.028454896638045568 1107 44-45 0.12552178103546652 1107 46-47 0.17380762239721292 1107 48-49 -0.09193400204346602 1107 50-51 0.11945667913732905 1107 52-53 0.18565239786278198 1107 54-55 0.14395343824340046 1107 56-57 -0.1603793452703428 1107 58-59 -0.011490540412488315 1107 60-61 -0.009367270804442995 1107 62-63 0.0988493373221857 1107 64-65 0.11685584787088743 1107 66-67 0.3123946935402735 1107 68-69 0.12759858148916337 1107 70-71 -0.040808737696604425 1107 72-73 0.026464131638970656 1107 74-75 0.048453682623883054 1107 76-77 -0.08687487998528454 1107 78-79 -0.1389929204597955 1107 80-81 0.27636881848988537 1107 82-83 0.17111955996395167 1107 84-85 0.21198044499908875 1107 86-87 0.18541404417389273 1107 88-89 0.32126608497473796 1107 90-91 0.5348266767016483 1107 92-93 0.5668023984632384 1107 94-95 0.8568209934144164 1107 96-97 0.7848290817046113 1107 98-99 0.8982277737408957 1107 100-101 1.1049446171984485 1108 1 0.003462878567596306 1108 2 0.08608357733756122 1108 3 0.06266803704600576 1108 4 0.04975067770222097 1108 5 0.05054790385529628 1108 6 0.0345374542069905 1108 7 0.07059867878577109 1108 8 0.07333043670865891 1108 9 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-1.223052298337553 2316 80-81 -1.8230780918484228 2316 82-83 -1.5195540958500438 2316 84-85 -1.379133430751228 2316 86-87 -1.603030000449703 2316 88-89 -1.4600397197219905 2316 90-91 -1.73068156754956 2316 92-93 -1.3715841750247577 2316 94-95 -1.3542720000363353 2316 96-97 -1.6413566385666805 2316 98-99 -1.7746194673085078 2316 100-101 -1.4338355159374139 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 14.0 4 34.0 5 93.0 6 213.0 7 527.0 8 1200.0 9 2066.0 10 3375.0 11 4934.0 12 6965.0 13 9676.0 14 12132.0 15 15926.0 16 19869.0 17 24932.0 18 21317.0 19 14892.0 20 13846.0 21 14470.0 22 15772.0 23 17620.0 24 20061.0 25 23755.0 26 28513.0 27 34952.0 28 41618.0 29 49488.0 30 59784.0 31 70974.0 32 85568.0 33 104478.0 34 130835.0 35 167020.0 36 207753.0 37 212680.0 38 88318.0 39 2928.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.145533171385495 19.99150559138884 15.344252596749875 20.51870864047579 2 16.16174964917388 22.59536091747981 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24.967233164126604 30.824245790357015 21.33882331160435 22-23 21.948350587965916 25.355707112833848 31.08610961189343 21.609832687306803 24-25 22.407636861779448 25.60235407101727 31.099264287387168 20.890744779816117 26-27 21.858046299049505 25.20311320856554 31.935640991168253 21.0031995012167 28-29 21.022189016188378 26.72093105415491 31.310124147532488 20.94675578212422 30-31 21.46787262291559 25.538416549482708 31.12651321046763 21.867197617134078 32-33 21.46655445043869 25.79562363252827 31.194922924395023 21.54289899263802 34-35 22.50515964053768 25.403096405867515 30.93841176957433 21.153332184020474 36-37 22.416083626693467 25.12526894753147 31.331581841670257 21.127065584104805 38-39 21.733717164447643 25.85315188589748 31.285943275867712 21.12718767378717 40-41 21.753861452206966 26.06135145920697 30.7329072270161 21.451879861569964 42-43 21.43647084722001 26.27601779021041 29.969445768283066 22.318065594286512 44-45 22.103340164236055 25.653108987516294 29.830873826739307 22.412677021508348 46-47 22.936960965275098 26.527173057970344 29.226504897780682 21.309361078973872 48-49 22.710416746396927 26.625327957142247 28.720099516450276 21.94415578001055 50-51 21.818177060402448 27.06609394104528 29.315117064864054 21.800611933688213 52-53 22.702851578382344 27.47468311161805 27.95182701268404 21.87063829731557 54-55 21.81525630969258 26.813421250444048 28.461147445211544 22.910174994651832 56-57 22.814693520810348 26.77804901743679 28.052043123725735 22.355214338027128 58-59 23.59016546267236 26.24656342354159 27.91051244854335 22.252758665242702 60-61 22.46902862421441 26.81023860217716 29.202900660332336 21.517832113276093 62-63 22.21990338518224 26.903837900642703 28.950622291540185 21.925636422634874 64-65 21.867871862065492 27.6670133042764 28.38657802601058 22.07853680764753 66-67 22.379552063536714 27.024038886144847 27.769607442274292 22.826801608044146 68-69 22.256400929759703 26.986615568699314 28.149600246505386 22.6073832550356 70-71 22.822054785334974 26.977157240028067 27.937936775584234 22.26285119905273 72-73 22.620657802503548 26.55255057031335 28.571437917112476 22.255353710070626 74-75 21.861730506346134 28.35911900947937 27.589032465516695 22.1901180186578 76-77 22.275474486506535 26.990883984316095 27.789798789525005 22.94384273965236 78-79 22.343523633167525 27.218139407541013 27.952620813752016 22.48571614553945 80-81 22.790394148693448 27.304712480312194 27.59381046385539 22.311082907138967 82-83 22.550004775755387 27.076047035302647 28.126418824588466 22.2475293643535 84-85 22.07766166483649 27.538884314808797 27.819380644477526 22.564073375877186 86-87 22.219772570559783 27.76717594706659 27.21306037962477 22.79999110274886 88-89 21.943200300426433 27.768018716462084 26.841256818803345 23.44752416430814 90-91 22.27646058379189 27.129027435516704 27.193930275899227 23.400581704792177 92-93 22.67615907470283 27.635042751817036 27.363354463917727 22.325443709562407 94-95 22.20584531344553 27.316880100747426 27.42502003499992 23.052254550807124 96-97 21.502287895009022 27.335839567025396 28.01757961675101 23.14429292121457 98-99 21.89640604898288 27.838163441342783 26.75200655259593 23.513423957078402 100-101 21.634030521171923 27.435558836815783 27.036552068616864 23.893858573395427 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1491.0 1 1119.5 2 1265.0 3 1562.0 4 1097.0 5 900.0 6 1360.5 7 2606.0 8 3870.5 9 4366.5 10 3813.5 11 2917.0 12 2702.0 13 2810.0 14 2970.5 15 3125.5 16 3246.5 17 3444.0 18 3608.0 19 3798.0 20 4146.0 21 4630.5 22 5207.5 23 5823.5 24 6618.0 25 7648.5 26 8949.0 27 10555.5 28 12457.0 29 14783.0 30 17570.5 31 20458.0 32 23956.5 33 26696.5 34 28366.5 35 30063.5 36 33004.0 37 36175.0 38 37454.5 39 38064.0 40 38722.0 41 40598.0 42 42053.5 43 43930.0 44 46406.5 45 48468.5 46 50590.0 47 60815.5 48 82668.5 49 80861.5 50 60207.0 51 50840.5 52 47097.0 53 46716.5 54 46671.0 55 43862.5 56 39586.5 57 36343.5 58 34453.0 59 31394.0 60 26583.0 61 22647.0 62 19611.0 63 15224.0 64 11002.5 65 8465.0 66 6419.5 67 5002.5 68 3781.5 69 2708.0 70 2142.0 71 1748.0 72 1518.0 73 1354.5 74 1120.0 75 946.0 76 694.5 77 430.0 78 300.0 79 219.0 80 153.5 81 114.5 82 68.0 83 45.5 84 32.0 85 13.0 86 8.5 87 5.0 88 2.0 89 2.5 90 2.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03532635005534462 2 0.0045139225070718126 3 0.0035326350055344615 4 3.270958338457835E-4 5 5.233533341532535E-4 6 1.3083833353831338E-4 7 0.0 8 0.0 9 0.0017008983359980741 10-11 3.9251500061494014E-4 12-13 8.504491679990371E-4 14-15 0.0021261229199975927 16-17 0.0023550900036896413 18-19 0.0 20-21 3.2709583384578345E-5 22-23 0.0018971558363055443 24-25 0.0011775450018448206 26-27 0.0055279195919937405 28-29 0.006541916676915669 30-31 0.0016027695858443392 32-33 4.906437507686752E-4 34-35 0.01010726126583471 36-37 0.012593189603062665 38-39 0.01007455168245013 40-41 0.0022242516701513277 42-43 6.869012510761453E-4 44-45 7.52320417845302E-4 46-47 2.2896708369204841E-4 48-49 8.177395846144587E-4 50-51 4.5793416738409683E-4 52-53 0.0019298654196901225 54-55 0.002813024171073738 56-57 0.0016681887526134957 58-59 7.52320417845302E-4 60-61 0.0036307637556881964 62-63 0.003925150006149402 64-65 0.007228817927991815 66-67 0.002551347503997111 68-69 0.0024205091704587976 70-71 9.812875015373503E-5 72-73 9.485779181527722E-4 74-75 0.0048737279243021734 76-77 0.001995284586459279 78-79 0.0027803145876891594 80-81 0.0036961829224573537 82-83 0.0032709583384578347 84-85 0.00415411708984145 86-87 0.002747605004304581 88-89 0.0071961083446072365 90-91 0.010826872100295433 92-93 9.158683347681937E-4 94-95 0.0018971558363055443 96-97 0.0010139970849219289 98-99 0.0032382487550732565 100-101 0.003827021255995667 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1528604.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.2140127194143 #Duplication Level Percentage of deduplicated Percentage of total 1 76.04976797209805 38.187640162594384 2 13.360137204508717 13.41732199040642 3 4.445271823390941 6.696448076430202 4 1.9894374285325007 3.9959054536323944 5 1.0440649676258411 2.621334578212944 6 0.6609445131655112 1.9913205714552038 7 0.43559173388380507 1.5310966205999181 8 0.31546774050767135 1.2672720907533679 9 0.2304481381099365 1.0414553164395923 >10 1.2995685854417034 12.206714994207301 >50 0.10648789602932128 3.677498057727787 >100 0.05471575482470609 5.0282173892679705 >500 0.004048120940642821 1.3534007296004344 >1k 0.003395198208281076 3.771096383723381 >5k 3.917536394170472E-4 1.506182885606685 >10k+ 2.6116909294469817E-4 1.7070946993419434 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14449 0.9452415406475451 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11585 0.7578810470206804 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9885 0.6466684635131139 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6662 0.4358224890161219 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6423 0.42018730815829347 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4515 0.29536753796274245 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4318 0.2824799621092186 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3894 0.2547422353990962 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3713 0.2429013662138788 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3449 0.22563070618682146 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3177 0.20783669282561082 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2731 0.17865974444656693 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2549 0.16675345609458042 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2546 0.16655719859427293 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2373 0.15523968274320882 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2317 0.15157620940413605 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2308 0.15098743690321365 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2084 0.13633354354692256 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1970 0.1288757585352387 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1856 0.12141797352355484 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.541916676915669E-5 0.0 16-17 0.0 0.0 0.0 6.541916676915669E-5 0.0 18-19 0.0 0.0 0.0 6.541916676915669E-5 0.0 20-21 0.0 0.0 0.0 6.541916676915669E-5 0.0 22-23 0.0 0.0 0.0 1.6354791692289172E-4 0.0 24-25 0.0 0.0 0.0 4.579341673840969E-4 0.0 26-27 0.0 0.0 0.0 6.869012510761453E-4 0.0 28-29 0.0 0.0 0.0 0.0018317366695363875 0.0 30-31 0.0 0.0 0.0 0.004023278756303137 0.0 32-33 0.0 0.0 0.0 0.0083082341796829 0.0 34-35 0.0 0.0 0.0 0.01367260585475375 0.0 36-37 0.0 0.0 0.0 0.02034536086520773 0.0 38-39 0.0 0.0 0.0 0.029176948379043884 0.0 40-41 0.0 0.0 0.0 0.04042904506333884 0.0 42-43 0.0 0.0 0.0 0.05298952508301692 0.0 44-45 0.0 0.0 0.0 0.0687228346909991 0.0 46-47 0.0 0.0 0.0 0.09067096514205117 0.0 48-49 0.0 0.0 0.0 0.1145162514294088 0.0 50-51 0.0 0.0 0.0 0.14509971189398954 0.0 52-53 0.0 0.0 0.0 0.1818652836182556 0.0 54-55 0.0 0.0 0.0 0.22294852034928603 0.0 56-57 0.0 0.0 0.0 0.2766249466833791 0.0 58-59 0.0 0.0 0.0 0.3376283196956177 0.0 60-61 0.0 0.0 0.0 0.41403790648199273 0.0 62-63 0.0 0.0 0.0 0.48848491826529306 0.0 64-65 0.0 0.0 0.0 0.5666608225544353 0.0 66-67 0.0 0.0 0.0 0.670710007300779 0.0 68-69 0.0 0.0 0.0 0.7881701212347998 0.0 70-71 0.0 0.0 0.0 0.924307407281415 0.0 72-73 0.0 0.0 0.0 1.0536410999840378 0.0 74-75 0.0 0.0 0.0 1.2046939560540206 0.0 76-77 0.0 0.0 0.0 1.3578075158772318 0.0 78-79 0.0 0.0 0.0 1.5306776640647284 0.0 80-81 0.0 0.0 0.0 1.7251361372860465 0.0 82-83 0.0 0.0 0.0 1.9464818880494885 0.0 84-85 0.0 0.0 0.0 2.173486396738462 0.0 86-87 0.0 0.0 0.0 2.420182074624952 0.0 88-89 0.0 0.0 0.0 2.695204251722487 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 10390 0.0 22.469166 12-13 GTACATG 11820 0.0 21.709717 1 AGTACTT 10645 0.0 21.596264 12-13 TACATGG 11795 0.0 21.222954 2 GTACTTT 11145 0.0 20.90441 14-15 ACATGGG 11700 0.0 19.933088 3 GTGTAAG 580 0.0 19.663513 1 TACCTGG 1065 0.0 19.624395 2 TACTTTT 12030 0.0 19.050688 14-15 GGACCGT 150 7.7261013E-4 18.99874 6 CCGAACC 125 0.0060343514 18.998735 4 ACTTTTT 12560 0.0 18.75733 16-17 AGAGTAC 19255 0.0 18.538067 10-11 ATGGGAT 2590 0.0 18.521935 5 CATGGGA 7485 0.0 17.831146 4 TAAGGTG 700 0.0 17.641684 5 CATGGGG 4050 0.0 17.356873 4 ATACCGT 165 0.0014647635 17.27158 6 TAGGACA 750 0.0 17.098866 4 AGTACAT 9525 0.0 16.406807 2 >>END_MODULE