FastQCFastQC Report
Thu 26 May 2016
SRR936416_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936416_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525355
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT122480.8029606222813704No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117980.7734592930825939No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89440.5863553074530191No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT79160.5189611598611471No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57960.37997712008024365No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT53940.3536225993293365No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC51460.337364089015344No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT36100.23666621868351956No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT33400.21896542116425355No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC29430.1929386929599995No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC28030.1837605016537134No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC26750.1753690124593947No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG23490.15399693841761428No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21600.14160638015412805No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA21540.14121302909814437No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19210.1259378964241111No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA18920.12403669965352328No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17990.1179397582857761No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17600.11538297642188211No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15700.1029268596490653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG119700.025.3704011
TACATGG119750.024.6065672
TAAGGTG5800.024.5882645
ACATGGG119550.023.4199583
GGTATAC1452.3214478E-522.9482943
GAGTACT89100.022.69638812-13
TAGCGCG1050.002186546622.6368164
GTAAGGT5300.022.423264
GTAATAC1754.0243394E-621.730633
AGTACTT90900.021.64568512-13
CATGGGG38100.021.4604154
GGTAAGG5100.021.4376933
GTACCTG12650.021.4143541
CATGGGA77350.021.387184
GTGTAGC6450.021.3677581
GTACTTT96100.021.18909614-15
TACCTGG12400.021.0802572
ATGGGAG28650.020.7405385
ACGCTCT1456.1148754E-419.6706129
GTAGCGC1707.910159E-519.5735453
Produced by FastQC (version 0.11.5)