##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936416_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1525355 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6869102602345 31.0 31.0 34.0 30.0 34.0 2 31.734278905566246 33.0 31.0 34.0 30.0 34.0 3 31.688844236259754 33.0 31.0 34.0 30.0 34.0 4 35.376447449937885 37.0 35.0 37.0 33.0 37.0 5 35.43632859236047 37.0 35.0 37.0 33.0 37.0 6 35.524567067994006 37.0 35.0 37.0 33.0 37.0 7 35.41820887596658 37.0 35.0 37.0 33.0 37.0 8 35.406488981253545 37.0 35.0 37.0 33.0 37.0 9 36.93565760101747 39.0 37.0 39.0 33.0 39.0 10-11 36.826628227527365 39.0 37.0 39.0 33.0 39.0 12-13 36.73499480448814 39.0 37.0 39.0 32.5 39.0 14-15 37.71964231277309 40.0 37.0 41.0 32.5 41.0 16-17 37.667900587076446 40.0 37.0 41.0 32.0 41.0 18-19 37.61528889996099 40.0 37.0 41.0 32.0 41.0 20-21 37.61275145785735 40.0 37.0 41.0 32.0 41.0 22-23 37.589070085324394 40.0 37.0 41.0 32.0 41.0 24-25 37.6791245316664 40.0 37.0 41.0 32.0 41.0 26-27 37.58340156881513 40.0 37.0 41.0 32.0 41.0 28-29 37.473251144815464 40.0 37.0 41.0 32.0 41.0 30-31 37.244811863467845 39.0 36.5 41.0 32.0 41.0 32-33 37.01043920923326 39.0 36.0 41.0 31.0 41.0 34-35 36.77228481238794 39.0 35.0 41.0 31.0 41.0 36-37 36.54028898190913 39.0 35.0 40.5 30.0 41.0 38-39 36.32251836457743 39.0 35.0 40.0 30.0 41.0 40-41 36.089005182400165 38.5 35.0 40.0 30.0 41.0 42-43 35.77040918343599 38.0 35.0 40.0 29.0 41.0 44-45 35.518010889268396 38.0 35.0 40.0 27.5 41.0 46-47 35.21294518325242 38.0 35.0 40.0 26.5 41.0 48-49 34.870469497264565 38.0 34.0 40.0 24.5 41.0 50-51 33.84349217067502 37.5 33.0 39.5 23.5 40.5 52-53 33.94186369730325 37.5 33.5 39.5 23.0 40.5 54-55 34.37417060290883 38.0 34.0 40.0 23.5 41.0 56-57 34.18889078280138 38.0 34.0 40.0 23.0 41.0 58-59 33.959408793362854 38.0 34.0 40.0 22.5 41.0 60-61 33.63117110443143 37.0 33.0 40.0 22.0 41.0 62-63 33.316037905930095 37.0 33.0 40.0 20.5 41.0 64-65 32.9641798138794 36.0 33.0 39.0 19.5 41.0 66-67 32.57088612159137 36.0 32.0 39.0 18.0 41.0 68-69 32.12735330464056 35.0 32.0 39.0 16.0 40.0 70-71 31.697824440867862 35.0 31.0 38.0 13.0 40.0 72-73 31.29867899603699 35.0 31.0 37.0 9.5 39.5 74-75 30.90667549521259 35.0 31.0 37.0 7.5 39.0 76-77 30.469287805133888 34.5 30.0 36.0 6.0 39.0 78-79 30.014800161273932 34.0 30.0 36.0 2.0 37.5 80-81 29.556395396481474 34.0 29.0 35.0 2.0 37.0 82-83 29.150168321472705 34.0 29.0 35.0 2.0 37.0 84-85 28.79216706930518 34.0 29.0 35.0 2.0 36.0 86-87 28.49946438697877 34.0 29.0 35.0 2.0 36.0 88-89 28.145865388712792 34.0 28.0 35.0 2.0 35.5 90-91 27.767272536557066 34.0 27.0 35.0 2.0 35.0 92-93 27.348389391322023 33.5 26.5 35.0 2.0 35.0 94-95 26.852716908522932 33.0 25.0 35.0 2.0 35.0 96-97 26.27732855630329 33.0 24.5 35.0 2.0 35.0 98-99 25.587490780834628 33.0 22.0 35.0 2.0 35.0 100-101 23.013817439219068 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.34062064268674774 1101 2 0.29628935394823586 1101 3 0.47710458630192676 1101 4 0.4157058258999413 1101 5 0.3211291730644916 1101 6 0.3233933104599558 1101 7 0.4407826119390421 1101 8 0.40113821405088856 1101 9 0.676484030929565 1101 10-11 0.5764589630075392 1101 12-13 0.5846679471024814 1101 14-15 0.8153054372464865 1101 16-17 0.811841368904588 1101 18-19 0.897796411643121 1101 20-21 0.7807801016831419 1101 22-23 0.7354003946299201 1101 24-25 0.6016220796767584 1101 26-27 0.5352806072004697 1101 28-29 0.5364848766608503 1101 30-31 0.38532900252820923 1101 32-33 0.4077304985143826 1101 34-35 0.5340932314269011 1101 36-37 0.5408613825567983 1101 38-39 0.6291426533423063 1101 40-41 0.5842685174725162 1101 42-43 0.49253601773458655 1101 44-45 0.5230240145205229 1101 46-47 0.748150303265966 1101 48-49 0.656376491287574 1101 50-51 0.48552110967914786 1101 52-53 0.5109725200956703 1101 54-55 0.5375136487940111 1101 56-57 0.7336883006319468 1101 58-59 0.7668501121035831 1101 60-61 0.5271439976696399 1101 62-63 0.6371289814790657 1101 64-65 0.5858320485629278 1101 66-67 0.6344240840357358 1101 68-69 0.7200906085995271 1101 70-71 0.5736306077829525 1101 72-73 0.6376010740185798 1101 74-75 0.36650618926577394 1101 76-77 0.44737694717525756 1101 78-79 0.45944198654839496 1101 80-81 0.4830449332404321 1101 82-83 0.602784183125781 1101 84-85 0.4187456655041508 1101 86-87 0.48915690752530594 1101 88-89 0.4783670315604951 1101 90-91 0.5370309775766238 1101 92-93 0.6681066926620112 1101 94-95 0.8105345828976418 1101 96-97 0.9626915512522096 1101 98-99 0.8354938936190202 1101 100-101 1.2036822846389654 1102 1 0.1683624933982486 1102 2 0.2719773247141397 1102 3 0.4439321257299582 1102 4 0.22869567990702677 1102 5 0.1464952207439083 1102 6 0.1943044611086009 1102 7 0.2459134941823109 1102 8 0.10122593054549611 1102 9 0.22448484062028484 1102 10-11 0.348642890646758 1102 12-13 0.33120811735061295 1102 14-15 0.36937182478293096 1102 16-17 0.25303935604186734 1102 18-19 0.2822627792924237 1102 20-21 0.3084001436592132 1102 22-23 0.006953970941488308 1102 24-25 0.3220763587856723 1102 26-27 0.2495787509489844 1102 28-29 -0.07586503757586627 1102 30-31 -0.08812876748410758 1102 32-33 0.18424165484506716 1102 34-35 0.10457538804523381 1102 36-37 0.07999465835948172 1102 38-39 -0.15374304176652487 1102 40-41 0.07458596595238731 1102 42-43 -0.0615694338277919 1102 44-45 0.2651215396369153 1102 46-47 0.10143292439108365 1102 48-49 0.39575373968860816 1102 50-51 0.35964438864245807 1102 52-53 0.16518308940057835 1102 54-55 0.3056029562598539 1102 56-57 0.31161925674174284 1102 58-59 0.20238381547977724 1102 60-61 0.2078879187745173 1102 62-63 0.1386400490278632 1102 64-65 0.30514281709436375 1102 66-67 0.2103752042854623 1102 68-69 0.2215306506466277 1102 70-71 0.03512058075775215 1102 72-73 0.20473615386900335 1102 74-75 0.12012049948385695 1102 76-77 0.08185485646858837 1102 78-79 0.09972445496867977 1102 80-81 0.20607033551111797 1102 82-83 0.04125301535999526 1102 84-85 -0.017132511555320917 1102 86-87 -0.129826788570778 1102 88-89 0.0089879009836622 1102 90-91 0.17119459902490775 1102 92-93 0.22402151888036315 1102 94-95 0.03675790838265769 1102 96-97 -0.061909906901345124 1102 98-99 -0.08839340311487476 1102 100-101 0.3472922978141959 1103 1 0.32947841824032764 1103 2 0.3642266798907734 1103 3 0.3892787080717959 1103 4 0.4343795573736102 1103 5 0.3820978334889631 1103 6 0.3793517900747787 1103 7 0.4483418153527623 1103 8 0.32521700676858245 1103 9 0.35999646756214077 1103 10-11 0.3408033035852256 1103 12-13 0.37010690617677966 1103 14-15 0.6236629969592542 1103 16-17 0.7846976367560217 1103 18-19 0.5947575131825289 1103 20-21 0.6956289940186906 1103 22-23 0.4894195541623745 1103 24-25 0.26743127258495036 1103 26-27 0.30726214676301566 1103 28-29 0.24932730905301526 1103 30-31 0.20040532358217433 1103 32-33 0.47083687428622056 1103 34-35 0.4018226116263435 1103 36-37 0.4161862579867375 1103 38-39 0.28106974881270474 1103 40-41 0.43523919623135754 1103 42-43 0.49741869966164387 1103 44-45 0.5801578396398028 1103 46-47 0.69949889444311 1103 48-49 0.8312105676480996 1103 50-51 0.7971960279588899 1103 52-53 0.7899263861928745 1103 54-55 0.8737899619529159 1103 56-57 1.042360071074107 1103 58-59 0.8078254833306673 1103 60-61 0.7798541050042758 1103 62-63 0.8098177021097541 1103 64-65 0.8474049995715163 1103 66-67 0.8685793633490206 1103 68-69 0.8050866801695378 1103 70-71 0.7687242725051391 1103 72-73 0.6880572048524911 1103 74-75 0.8526646813598866 1103 76-77 0.7734813644623841 1103 78-79 0.5886607510730819 1103 80-81 0.7402950195130806 1103 82-83 0.6464628171782039 1103 84-85 0.8108115739901969 1103 86-87 0.8033564946899752 1103 88-89 0.8279137340350893 1103 90-91 0.9949773573723988 1103 92-93 0.9694374881149663 1103 94-95 0.7818382613597841 1103 96-97 0.924443637394976 1103 98-99 1.1347926656822125 1103 100-101 1.542937869440209 1104 1 0.17126005350404583 1104 2 0.1377980028917989 1104 3 0.270628312838042 1104 4 0.31598653550451417 1104 5 0.20422353362096146 1104 6 0.25574759463411567 1104 7 0.14429086052306417 1104 8 0.27338557867768287 1104 9 0.45593302630187793 1104 10-11 0.2937192673592861 1104 12-13 0.3127566031897189 1104 14-15 0.387043226607652 1104 16-17 0.3901162379700125 1104 18-19 0.32276546882257406 1104 20-21 0.42194363677936764 1104 22-23 0.2023386777678482 1104 24-25 0.2902292013664365 1104 26-27 0.0375813661827209 1104 28-29 -0.06567156971648558 1104 30-31 -0.029976965053137405 1104 32-33 -0.026277231303112103 1104 34-35 -0.017545625952202215 1104 36-37 0.1275369330184617 1104 38-39 -0.046551891955239455 1104 40-41 -0.05239031863258958 1104 42-43 0.3078657648408125 1104 44-45 0.12512109385843218 1104 46-47 0.3925448445075759 1104 48-49 0.4040637934792102 1104 50-51 0.2265349351668675 1104 52-53 0.2592014851924276 1104 54-55 0.2768576740820734 1104 56-57 0.3666320357247699 1104 58-59 0.2998532796540516 1104 60-61 0.27949480159421114 1104 62-63 0.10188932835265518 1104 64-65 0.06409553485715236 1104 66-67 0.3670663392374678 1104 68-69 0.1865184660349044 1104 70-71 0.016843663167332323 1104 72-73 0.18587972425742194 1104 74-75 0.21760482294072148 1104 76-77 0.21761669188717292 1104 78-79 0.05580041562129878 1104 80-81 0.2135817028622995 1104 82-83 0.1475228896504639 1104 84-85 0.09475045673496751 1104 86-87 0.18562092161377564 1104 88-89 0.12742752771848487 1104 90-91 0.21823537289634132 1104 92-93 0.1380457097924257 1104 94-95 0.3254700543602489 1104 96-97 0.16331223937343609 1104 98-99 0.03535098750594656 1104 100-101 0.7106166165160879 1105 1 0.31993393927458413 1105 2 0.3187396725671441 1105 3 0.35506533442522326 1105 4 0.3964215778554916 1105 5 0.39068862295931694 1105 6 0.3876278430516322 1105 7 0.43367623235712927 1105 8 0.20196164550417706 1105 9 0.3761440928816171 1105 10-11 0.4637897484279776 1105 12-13 0.544039898903705 1105 14-15 0.7073772162231577 1105 16-17 0.698210197143716 1105 18-19 0.659148934148277 1105 20-21 0.5623918091027846 1105 22-23 0.5037286885991037 1105 24-25 0.277326828101188 1105 26-27 0.4082758131524358 1105 28-29 0.44477266751133016 1105 30-31 0.2252341145137251 1105 32-33 0.2413806984989435 1105 34-35 0.06470696302900336 1105 36-37 0.22978578171078823 1105 38-39 -0.0723426773379785 1105 40-41 -0.016222179660083214 1105 42-43 -0.14029779227781347 1105 44-45 0.1733906258288087 1105 46-47 0.33116871690139504 1105 48-49 0.3695170957127374 1105 50-51 0.27309309016548156 1105 52-53 0.400725639926101 1105 54-55 0.343499102456839 1105 56-57 0.2969610382884227 1105 58-59 0.4079034722529542 1105 60-61 0.2896754004040716 1105 62-63 0.5078928166915233 1105 64-65 0.4893170300895804 1105 66-67 0.49051795054872827 1105 68-69 0.518554494887077 1105 70-71 0.6936229627462893 1105 72-73 0.49226392984863665 1105 74-75 0.3943919147881907 1105 76-77 0.38640761753075026 1105 78-79 0.32343237119497914 1105 80-81 0.38814280333840756 1105 82-83 0.4057680837688622 1105 84-85 0.42128092320613675 1105 86-87 0.4837946736170373 1105 88-89 0.3343541401336445 1105 90-91 0.36325760683582686 1105 92-93 0.4255447032910773 1105 94-95 0.5129218396988371 1105 96-97 0.6290340501927396 1105 98-99 0.9157705102554736 1105 100-101 1.3546041021328605 1106 1 0.10754327071417435 1106 2 0.017312136575927894 1106 3 0.0843775891193701 1106 4 0.1708085696364634 1106 5 0.31590464840373045 1106 6 0.23419751724052418 1106 7 0.3021678736425599 1106 8 0.3444143816962253 1106 9 0.4113440788153042 1106 10-11 0.4546150571218064 1106 12-13 0.45626509285284556 1106 14-15 0.39642186571457927 1106 16-17 0.2208756929195843 1106 18-19 0.4259521613264923 1106 20-21 0.06277847006477799 1106 22-23 0.03703476843978848 1106 24-25 -0.2566042586586903 1106 26-27 -0.12872951316336412 1106 28-29 0.005264676083967856 1106 30-31 0.02059636455165048 1106 32-33 0.10057440538420792 1106 34-35 0.2411567218502526 1106 36-37 0.050358326470657744 1106 38-39 0.20763697859725738 1106 40-41 0.053414889778693464 1106 42-43 0.11455459928942702 1106 44-45 0.03151177590666521 1106 46-47 0.1436809679487112 1106 48-49 0.3647783417671633 1106 50-51 0.16811032466208076 1106 52-53 0.29434910410876824 1106 54-55 0.442413325914643 1106 56-57 0.49175708643431193 1106 58-59 0.4783459361249811 1106 60-61 0.4417482613305026 1106 62-63 0.5550328931315534 1106 64-65 0.4759797503723675 1106 66-67 0.1596403620367397 1106 68-69 0.43517687869830013 1106 70-71 0.4089252290516612 1106 72-73 0.48203393902430136 1106 74-75 0.6584295799616129 1106 76-77 0.4567139767441546 1106 78-79 0.6215803710100367 1106 80-81 0.6312776583088002 1106 82-83 0.5945911561784918 1106 84-85 0.8317911333347965 1106 86-87 0.9472834593095065 1106 88-89 0.9137350658146381 1106 90-91 1.0697169776415762 1106 92-93 0.9943949380227721 1106 94-95 1.1668990262136631 1106 96-97 1.0126684743821208 1106 98-99 1.0811055979713444 1106 100-101 1.7020915059397517 1107 1 0.15346052822913236 1107 2 0.11610751778459871 1107 3 0.16466612824968152 1107 4 0.24744826762469074 1107 5 0.2889603623987398 1107 6 0.2081744633810203 1107 7 0.31698377256564925 1107 8 0.2759364596912093 1107 9 0.401736800379652 1107 10-11 0.3692209806077429 1107 12-13 0.3035771299186578 1107 14-15 0.07024136369151535 1107 16-17 0.20198876322226766 1107 18-19 0.5309176593949729 1107 20-21 0.2569731704905749 1107 22-23 0.19821915153335823 1107 24-25 0.17729357579418803 1107 26-27 0.16789286953657978 1107 28-29 0.245440622401901 1107 30-31 0.19630477269124924 1107 32-33 0.2072899772147565 1107 34-35 0.08681825975659052 1107 36-37 0.1577999909570451 1107 38-39 0.29620725103403345 1107 40-41 0.3619920722919261 1107 42-43 0.20673795957139163 1107 44-45 0.1513887377852967 1107 46-47 0.10246192754714656 1107 48-49 0.16519241172534294 1107 50-51 0.10570127419947539 1107 52-53 0.1929383859386391 1107 54-55 0.19356920995500815 1107 56-57 0.4161814667077124 1107 58-59 0.47713851053277523 1107 60-61 0.373966693257735 1107 62-63 0.37960700286610916 1107 64-65 0.4711822927993339 1107 66-67 0.4338283862861587 1107 68-69 0.39371241029135007 1107 70-71 0.45980734976348003 1107 72-73 0.45677089333125664 1107 74-75 0.32751560874268293 1107 76-77 0.43900790827358804 1107 78-79 0.5152981826008514 1107 80-81 0.6675555596637857 1107 82-83 0.36516291822459834 1107 84-85 0.17879458937544968 1107 86-87 0.48124257558156813 1107 88-89 0.24152830884249354 1107 90-91 0.2862150524769014 1107 92-93 0.4817402757761471 1107 94-95 0.4980305245006811 1107 96-97 0.633581947728036 1107 98-99 0.8513392073401782 1107 100-101 1.4898667854419863 1108 1 0.24058819171090207 1108 2 0.23526709669956247 1108 3 0.3718047753417366 1108 4 0.3802806923616018 1108 5 0.3454601936219248 1108 6 0.2521886581232309 1108 7 0.4278836065872085 1108 8 0.4022343359017242 1108 9 0.006847327330596897 1108 10-11 0.32676827746779935 1108 12-13 0.265276686201787 1108 14-15 0.8107427270233956 1108 16-17 0.8080909632673894 1108 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76-77 -4.197321809256991 2316 78-79 -3.999017814141286 2316 80-81 -4.946400240787586 2316 82-83 -4.351939665770015 2316 84-85 -4.2581372184652615 2316 86-87 -5.22290846911849 2316 88-89 -6.344028690101883 2316 90-91 -5.477295010041299 2316 92-93 -4.755021813805971 2316 94-95 -4.297646096240438 2316 96-97 -5.782329878404024 2316 98-99 -7.290096845732041 2316 100-101 -7.762256399073156 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2843.0 3 1011.0 4 1012.0 5 1078.0 6 1276.0 7 1420.0 8 1745.0 9 2390.0 10 2949.0 11 4193.0 12 5954.0 13 7638.0 14 8888.0 15 11538.0 16 15546.0 17 20998.0 18 15620.0 19 9974.0 20 9428.0 21 9906.0 22 10628.0 23 11891.0 24 13530.0 25 15655.0 26 18192.0 27 22028.0 28 26488.0 29 32875.0 30 40256.0 31 50734.0 32 65234.0 33 85660.0 34 115979.0 35 162374.0 36 218844.0 37 272478.0 38 199005.0 39 28092.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.2785394418662 18.37276798307938 14.314102135152403 21.03459043990201 2 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21.903731073221135 25.783753520498877 29.563864603055556 22.748650803224436 46-47 22.735980112118455 26.42090246769432 29.105956596219496 21.73716082396773 48-49 22.466785480966823 26.5232656515769 28.71341944911642 22.296529418339865 50-51 21.70511812752935 26.98511088981298 29.294591445989525 22.015179536668146 52-53 22.434464393183482 27.441924956436342 27.920076257708047 22.20353439267213 54-55 21.583212295895805 26.780790957118494 28.53969355975939 23.096303187226315 56-57 22.593768920390048 26.79253434803716 27.884938459869186 22.728758271703605 58-59 23.33855305612748 26.285505615436133 27.81562632248184 22.56031500595455 60-61 22.341599367459573 26.655173679814432 29.219952034973296 21.7832749177527 62-63 22.038213595695595 26.87709668981152 28.978752669110303 22.10593704538258 64-65 21.70927400942804 27.654983942961042 28.37642799802143 22.259314049589484 66-67 22.10059986232668 27.07467138689481 27.718884190513656 23.105844560264856 68-69 21.934733524069767 26.99162009708152 28.281156166984417 22.792490211864298 70-71 22.603369949345538 26.880333461283413 27.899290286819056 22.617006302552 72-73 22.369414564271505 26.61193100578908 28.609535637577444 22.40911879236197 74-75 21.670811128905616 28.315927684734554 27.710535676525407 22.302725509834424 76-77 21.99741373148579 27.031555230013304 27.851633172172434 23.119397866328477 78-79 22.174940960352043 27.23479370466701 27.96966232567198 22.620603009308972 80-81 22.618844677101254 27.36043651126684 27.57939369938316 22.44132511224875 82-83 22.254540471860285 27.067748783503642 28.22176489331153 22.45594585132454 84-85 21.985893253025555 27.532250244788152 27.796886969993086 22.684969532193204 86-87 22.011066421474627 27.76350768847499 27.50654774197144 22.718878148078947 88-89 21.8662232222231 27.733044347066738 26.901651574870257 23.49908085583991 90-91 22.08251953925323 27.080182237423973 27.350627399995936 23.486670823326865 92-93 22.615889567414676 27.433808767625536 27.507831749499413 22.44246991546038 94-95 22.180842309969492 27.11542962948878 27.62576852441346 23.07795953612827 96-97 21.401516144937265 27.181081102345683 28.316747356384543 23.10065539633251 98-99 21.82385037629533 27.740477206870302 27.033525923623557 23.402146493210815 100-101 21.71833280001679 26.991314371744362 27.53106787458493 23.759284953653918 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 904.0 1 731.0 2 1134.5 3 1486.0 4 1020.0 5 899.5 6 1481.5 7 2839.5 8 4360.5 9 4793.0 10 3884.0 11 2926.5 12 2729.5 13 2851.5 14 3025.0 15 3103.0 16 3142.0 17 3268.0 18 3419.5 19 3545.0 20 3749.0 21 4121.5 22 4703.5 23 5415.5 24 6060.0 25 7039.5 26 8619.0 27 10350.5 28 11970.5 29 14178.5 30 16915.0 31 19816.5 32 23549.0 33 26273.0 34 28037.0 35 29705.5 36 32686.0 37 36022.0 38 37214.0 39 37903.5 40 38555.5 41 40160.5 42 41670.5 43 43658.5 44 46188.0 45 48662.0 46 51146.0 47 60235.0 48 81439.5 49 80521.0 50 60381.5 51 51667.0 52 47905.0 53 47067.0 54 46942.0 55 44243.5 56 40297.5 57 36827.0 58 35042.0 59 32072.0 60 27029.0 61 23158.0 62 20240.0 63 15672.5 64 11125.0 65 8690.0 66 6668.5 67 5127.0 68 3826.5 69 2753.0 70 2202.5 71 1811.0 72 1493.0 73 1368.0 74 1141.5 75 935.5 76 698.5 77 413.5 78 293.0 79 194.0 80 124.5 81 95.0 82 60.0 83 37.0 84 23.5 85 15.0 86 14.0 87 10.5 88 7.0 89 5.5 90 3.0 91 4.0 92 5.0 93 2.0 94 1.5 95 2.0 96 1.5 97 0.5 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06955757839978235 2 0.028649068577478686 3 0.015799600748678175 4 0.018880850687217076 5 0.022093217644417202 6 0.02799348348417254 7 0.07722792399146428 8 0.055659174421692 9 0.06896755181580681 10-11 0.05946156796286766 12-13 0.04005624920100567 14-15 0.038941754542385215 16-17 0.04654654162473654 18-19 0.04467812410881401 20-21 0.01894640919654769 22-23 0.012587233791478048 24-25 0.022584906464396814 26-27 0.02461722025364587 28-29 0.006129720622412487 30-31 7.86702111967378E-4 32-33 0.00953876310760446 34-35 0.010653257766224913 36-37 0.004162965342494042 38-39 0.009702659380930997 40-41 0.004097406833163428 42-43 0.0017373004972612933 44-45 1.966755279918445E-4 46-47 2.6223403732245933E-4 48-49 3.605718013183816E-4 50-51 7.86702111967378E-4 52-53 6.555850933061483E-5 54-55 3.2779254665307416E-5 56-57 0.003245146211865435 58-59 0.0033107047211960497 60-61 0.005048005218457343 62-63 0.002228989317240905 64-65 7.539228573020707E-4 66-67 3.2779254665307424E-4 68-69 0.0025567818638939786 70-71 0.0013439494412776042 72-73 0.021535970315106973 74-75 0.014914560872714876 76-77 0.02478111652697241 78-79 0.021011502240462054 80-81 0.011210505095535138 82-83 0.004785771181134883 84-85 0.003704055777179738 86-87 0.001311170186612297 88-89 0.0015078457146041413 90-91 0.006031382858416565 92-93 0.010030451927584071 94-95 0.012587233791478048 96-97 0.011210505095535138 98-99 0.023338829321698885 100-101 0.020126462364498755 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1525355.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.13602824918587 #Duplication Level Percentage of deduplicated Percentage of total 1 76.52485832841258 39.131773172466524 2 13.092068379293096 13.389527569676096 3 4.361868018676075 6.691458186667205 4 1.95606714032396 4.001020181796409 5 1.0418322999124259 2.663758295961805 6 0.6404477840592625 1.9649973586669767 7 0.4238348100441663 1.517126017358477 8 0.314733958270208 1.2875395664866753 9 0.22293278231697086 1.0259907348807202 >10 1.2588023970335886 11.94607973957325 >50 0.10263549929936176 3.5938314925384263 >100 0.05218727357978162 4.872594782676974 >500 0.004123375348565612 1.4258936715460002 >1k 0.002705965072496182 2.71664372385259 >5k 6.442773982133768E-4 2.1873367061294373 >10k+ 2.577109592853507E-4 1.5844287997225104 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12248 0.8029606222813704 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11798 0.7734592930825939 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8944 0.5863553074530191 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7916 0.5189611598611471 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5796 0.37997712008024365 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5394 0.3536225993293365 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5146 0.337364089015344 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3610 0.23666621868351956 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3340 0.21896542116425355 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2943 0.1929386929599995 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2803 0.1837605016537134 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2675 0.1753690124593947 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2349 0.15399693841761428 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2160 0.14160638015412805 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2154 0.14121302909814437 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1921 0.1259378964241111 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1892 0.12403669965352328 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1799 0.1179397582857761 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1760 0.11538297642188211 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1570 0.1029268596490653 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.277925466530742E-5 0.0 12-13 0.0 0.0 0.0 6.555850933061485E-5 0.0 14-15 0.0 0.0 0.0 6.555850933061485E-5 0.0 16-17 0.0 0.0 0.0 1.311170186612297E-4 0.0 18-19 0.0 0.0 0.0 1.311170186612297E-4 0.0 20-21 0.0 0.0 0.0 1.311170186612297E-4 0.0 22-23 0.0 0.0 0.0 2.2945478265715195E-4 0.0 24-25 0.0 0.0 0.0 5.244680746449187E-4 0.0 26-27 0.0 0.0 0.0 7.867021119673781E-4 0.0 28-29 0.0 0.0 0.0 0.0015406249692694488 0.0 30-31 0.0 0.0 0.0 0.004294082361155272 0.0 32-33 0.0 0.0 0.0 0.00901429503295954 0.0 34-35 0.0 0.0 0.0 0.014258975779408729 0.0 36-37 0.0 0.0 0.0 0.02160152882443759 0.0 38-39 0.0 0.0 0.0 0.031074733422711436 0.0 40-41 0.0 0.0 0.0 0.0437275257235201 0.0 42-43 0.0 0.0 0.0 0.05710146162696553 0.0 44-45 0.0 0.0 0.0 0.07454002510890907 0.0 46-47 0.0 0.0 0.0 0.09817386772259573 0.0 48-49 0.0 0.0 0.0 0.12577400015078458 0.0 50-51 0.0 0.0 0.0 0.15622592773485516 0.0 52-53 0.0 0.0 0.0 0.19205365308403619 0.0 54-55 0.0 0.0 0.0 0.2341749953289562 0.0 56-57 0.0 0.0 0.0 0.2881296485080522 0.0 58-59 0.0 0.0 0.0 0.35080358342812 0.0 60-61 0.0 0.0 0.0 0.4255075048103556 0.0 62-63 0.0 0.0 0.0 0.5005392187392443 0.0 64-65 0.0 0.0 0.0 0.5798650150292883 0.0 66-67 0.0 0.0 0.0 0.6768260503292676 0.0 68-69 0.0 0.0 0.0 0.7963392128389785 0.0 70-71 0.0 0.0 0.0 0.9321436649173471 0.0 72-73 0.0 0.0 0.0 1.0584421331427767 0.0 74-75 0.0 0.0 0.0 1.208177768453901 0.0 76-77 0.0 0.0 0.0 1.358634547367662 0.0 78-79 0.0 0.0 0.0 1.5316434534911545 0.0 80-81 0.0 0.0 0.0 1.731072438874885 0.0 82-83 0.0 0.0 0.0 1.9572165168108409 0.0 84-85 0.0 0.0 0.0 2.182442775616168 0.0 86-87 0.0 0.0 0.0 2.4239275447354878 0.0 88-89 0.0 0.0 0.0 2.706025810385124 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11970 0.0 25.370401 1 TACATGG 11975 0.0 24.606567 2 TAAGGTG 580 0.0 24.588264 5 ACATGGG 11955 0.0 23.419958 3 GGTATAC 145 2.3214478E-5 22.948294 3 GAGTACT 8910 0.0 22.696388 12-13 TAGCGCG 105 0.0021865466 22.636816 4 GTAAGGT 530 0.0 22.42326 4 GTAATAC 175 4.0243394E-6 21.73063 3 AGTACTT 9090 0.0 21.645685 12-13 CATGGGG 3810 0.0 21.460415 4 GGTAAGG 510 0.0 21.437693 3 GTACCTG 1265 0.0 21.414354 1 CATGGGA 7735 0.0 21.38718 4 GTGTAGC 645 0.0 21.367758 1 GTACTTT 9610 0.0 21.189096 14-15 TACCTGG 1240 0.0 21.080257 2 ATGGGAG 2865 0.0 20.740538 5 ACGCTCT 145 6.1148754E-4 19.670612 9 GTAGCGC 170 7.910159E-5 19.573545 3 >>END_MODULE