FastQCFastQC Report
Thu 26 May 2016
SRR936416_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936416_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525355
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT142330.9330942633026411No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114670.7517594264941604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98510.6458168754158867No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67640.4434377571122788No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT61050.4002346994634036No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42970.281704914593652No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC42710.280000393351056No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT38500.25240026092286716No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC37210.24394321321921783No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC34840.22840584650786208No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT31070.20369028849022028No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG27800.18225265593910925No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA25840.16940318811030874No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25260.1656007945691331No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23340.15301356077765504No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC22860.14986675232978552No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA22660.14855558214317321No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22420.14698217791923848No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20250.13275598139449507No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC18360.12036542313100884No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15430.1011567798971387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG118000.023.5953671
TACATGG119250.023.1036742
GAGTACT102850.022.0500412-13
AGTACTT104650.022.0111512-13
ACATGGG120400.021.4605663
CATGGGA75800.020.9901644
GTACTTT108500.020.63849314-15
GGTATAG2601.4864781E-820.1015931
TAAGGTG6400.020.03665
ATATAGG5250.019.905582
TACTTTT116600.019.69354614-15
CGTATAG1456.136107E-419.6604921
ATGGGAG28600.019.0973035
GTGTAGC6000.019.0051421
AGAGTAC189950.019.00266610-11
TATCACG1250.00603059719.000782
CCTATAC3609.640644E-1118.4711653
ACTTTTT122650.018.4123416-17
GTAAGCG1555.5551936E-918.396994-95
ATGGGAT29000.018.17885
Produced by FastQC (version 0.11.5)