##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936416_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1525355 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.503327422141076 31.0 31.0 34.0 30.0 34.0 2 31.646106644027128 31.0 31.0 34.0 30.0 34.0 3 31.70109777723874 31.0 31.0 34.0 30.0 34.0 4 35.48793625090553 37.0 35.0 37.0 33.0 37.0 5 35.3083098688502 37.0 35.0 37.0 33.0 37.0 6 35.41881725893317 37.0 35.0 37.0 33.0 37.0 7 35.35081800630017 37.0 35.0 37.0 33.0 37.0 8 35.32546653074203 37.0 35.0 37.0 32.0 37.0 9 36.85240157209305 39.0 37.0 39.0 33.0 39.0 10-11 36.716534183845724 39.0 35.0 39.0 32.0 39.0 12-13 36.59578589902023 38.0 35.0 39.0 32.0 39.0 14-15 37.51020713211023 39.0 36.0 41.0 32.0 41.0 16-17 37.422262030805946 39.0 36.0 40.0 32.0 41.0 18-19 37.64111075782359 39.0 36.5 41.0 32.0 41.0 20-21 37.67511628440593 39.0 37.0 41.0 32.0 41.0 22-23 37.560632770732056 39.0 36.0 41.0 32.0 41.0 24-25 37.38296134342497 39.0 36.0 41.0 32.0 41.0 26-27 37.109284723883945 39.0 36.0 40.5 31.0 41.0 28-29 36.984586866663825 39.0 36.0 40.0 31.0 41.0 30-31 36.8781647550898 39.0 35.5 40.0 31.0 41.0 32-33 36.6273254422741 38.5 35.0 40.0 30.0 41.0 34-35 36.3573777907438 38.0 35.0 40.0 30.0 41.0 36-37 36.08003710611628 38.0 35.0 40.0 30.0 41.0 38-39 35.852353058796155 38.0 35.0 40.0 29.5 41.0 40-41 35.614584801570786 38.0 35.0 40.0 28.5 41.0 42-43 35.24581720320843 38.0 34.0 40.0 27.0 41.0 44-45 34.840320122201064 38.0 33.5 40.0 25.5 41.0 46-47 34.30029304653671 38.0 33.0 40.0 23.5 41.0 48-49 33.76876989291017 38.0 33.0 40.0 22.5 41.0 50-51 33.39117189113354 37.0 33.0 40.0 20.5 41.0 52-53 33.00892087415716 37.0 32.0 40.0 18.0 41.0 54-55 32.77588397455018 37.0 31.5 40.0 18.0 41.0 56-57 32.39726293223545 36.0 31.0 39.0 15.0 40.5 58-59 31.976507108181373 36.0 31.0 39.0 12.5 40.0 60-61 31.466038397618917 35.0 30.0 39.0 8.0 40.0 62-63 31.1051171694458 35.0 29.5 38.0 8.0 40.0 64-65 30.640114268481764 34.5 29.0 38.0 7.0 40.0 66-67 30.145568408665525 34.0 28.5 37.5 4.0 39.5 68-69 29.735745777212518 34.0 28.0 37.0 2.0 39.0 70-71 29.3027718137745 34.0 27.5 36.0 2.0 39.0 72-73 28.793392685637116 33.5 26.5 36.0 2.0 39.0 74-75 28.26177348879441 33.0 26.0 35.0 2.0 38.0 76-77 26.928508445574963 31.5 25.5 34.0 2.0 36.0 78-79 27.671363059746746 32.5 26.0 35.0 2.0 37.0 80-81 27.409556136112577 33.0 26.0 35.0 2.0 36.0 82-83 27.02512333194568 32.5 25.0 35.0 2.0 36.0 84-85 26.58736490849671 32.0 25.0 35.0 2.0 35.5 86-87 26.152416322757652 32.0 24.0 34.0 2.0 35.0 88-89 25.569931589695514 31.5 21.5 34.0 2.0 35.0 90-91 24.95077998236476 31.0 19.5 34.0 2.0 35.0 92-93 24.205287949362607 31.0 15.5 34.0 2.0 35.0 94-95 23.497143943541012 30.5 4.5 34.0 2.0 35.0 96-97 22.576684443949112 30.0 2.0 34.0 2.0 35.0 98-99 21.495942583857527 29.5 2.0 34.0 2.0 35.0 100-101 18.297967030625657 24.0 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22094821668228448 1101 2 0.30002192107630776 1101 3 0.372085775545802 1101 4 0.2984653758845397 1101 5 0.31240727088983533 1101 6 0.2617007643266973 1101 7 0.19019329766692294 1101 8 0.20913837910388366 1101 9 0.20236451514801246 1101 10-11 0.16871692103077152 1101 12-13 0.40645058232438913 1101 14-15 0.5166745255009957 1101 16-17 0.5263575379590364 1101 18-19 0.5387712948486225 1101 20-21 0.5082009833047962 1101 22-23 0.4206135239598794 1101 24-25 0.4367378782815905 1101 26-27 0.4565643520698188 1101 28-29 0.4878186703793901 1101 30-31 0.5728236231274195 1101 32-33 0.6040413701017755 1101 34-35 0.5182783489484848 1101 36-37 0.6780309573058787 1101 38-39 0.5133492195532057 1101 40-41 0.6756225984896318 1101 42-43 0.8250913397113564 1101 44-45 0.8176605881485202 1101 46-47 0.6451806998213101 1101 48-49 0.9679169411418584 1101 50-51 0.7945769970315837 1101 52-53 0.8842196357380487 1101 54-55 0.9810592819065462 1101 56-57 0.9358901718833863 1101 58-59 1.0064496700753551 1101 60-61 1.040918514711887 1101 62-63 0.8711049254627063 1101 64-65 0.9951821761753052 1101 66-67 0.8304504243343445 1101 68-69 0.7515923390309531 1101 70-71 0.9063672090510835 1101 72-73 1.2115317139330486 1101 74-75 1.0834719098044125 1101 76-77 0.8148763320589758 1101 78-79 0.908125328185104 1101 80-81 0.7137604695781015 1101 82-83 0.8685691008769219 1101 84-85 0.7200342244618874 1101 86-87 0.9130880007806859 1101 88-89 0.9398243975117602 1101 90-91 0.8815001370251494 1101 92-93 0.9221044249660686 1101 94-95 1.1099297033892839 1101 96-97 1.2728389936067224 1101 98-99 1.113384600209713 1101 100-101 1.440007881262897 1102 1 0.19098966006253093 1102 2 0.21806914593239313 1102 3 0.2506321676278773 1102 4 0.26829019148458144 1102 5 0.3059830142604909 1102 6 0.23352494779977917 1102 7 0.18381520761369075 1102 8 0.25765945059460194 1102 9 0.28058134890217445 1102 10-11 0.26768030383337305 1102 12-13 0.1828658542277637 1102 14-15 0.40240869835576376 1102 16-17 0.4805301086205276 1102 18-19 0.26270937481623235 1102 20-21 0.3447340154899976 1102 22-23 0.19122956364893184 1102 24-25 0.22146846079724014 1102 26-27 0.3366608757266434 1102 28-29 0.30171587517077825 1102 30-31 0.526145663405984 1102 32-33 0.496277927637756 1102 34-35 0.2912595378443541 1102 36-37 0.4006852513301453 1102 38-39 0.35751216625921955 1102 40-41 0.3281445364914717 1102 42-43 0.24331790395924457 1102 44-45 0.15846757989891813 1102 46-47 0.26294808420067284 1102 48-49 0.5276902277404147 1102 50-51 0.6480922266747839 1102 52-53 0.6806747529478869 1102 54-55 0.6450766973595634 1102 56-57 0.4097918512813763 1102 58-59 0.627422080219219 1102 60-61 0.6038186990230621 1102 62-63 0.8601776520251789 1102 64-65 0.638353606877498 1102 66-67 0.4233269216407045 1102 68-69 0.48825641326680724 1102 70-71 0.7006471702711572 1102 72-73 0.7329618834354079 1102 74-75 0.4525878908974228 1102 76-77 0.35923708478724237 1102 78-79 0.6172403859465909 1102 80-81 0.5161941583940717 1102 82-83 0.6649727245980195 1102 84-85 0.7203023457308326 1102 86-87 0.9885117566802428 1102 88-89 0.9376616709848058 1102 90-91 0.9873725255062311 1102 92-93 0.7971119714256254 1102 94-95 0.828199948320723 1102 96-97 0.8991223356406763 1102 98-99 1.122284095573523 1102 100-101 1.76266075477713 1103 1 0.26762617838104674 1103 2 0.34622299556876257 1103 3 0.21655241954599802 1103 4 0.30730593510960347 1103 5 0.42908737189569024 1103 6 0.3014033751727325 1103 7 0.2664007009428673 1103 8 0.1581095379334272 1103 9 0.1973416003675652 1103 10-11 0.2884125854957347 1103 12-13 0.2785015538626894 1103 14-15 0.4539557399652665 1103 16-17 0.4703988991695809 1103 18-19 0.3423862463130902 1103 20-21 0.19644759193001704 1103 22-23 0.1159153891045861 1103 24-25 0.15513025267051717 1103 26-27 0.19393766497786658 1103 28-29 0.1053490822851515 1103 30-31 0.060008950791591076 1103 32-33 0.014830354198316797 1103 34-35 0.23678501072529912 1103 36-37 -0.011555105872446347 1103 38-39 0.04512668449977042 1103 40-41 0.12133449749395453 1103 42-43 0.15739353975948944 1103 44-45 0.19975340988185053 1103 46-47 0.3346827187923793 1103 48-49 0.44736240218163204 1103 50-51 0.5181938012888025 1103 52-53 0.5172307359403945 1103 54-55 0.5780884783547933 1103 56-57 0.5172237942778608 1103 58-59 0.6270429203067991 1103 60-61 0.568672681585479 1103 62-63 0.7592008593784918 1103 64-65 0.7331266076735439 1103 66-67 0.5663473925032356 1103 68-69 0.6477051034359391 1103 70-71 0.6445728461252713 1103 72-73 0.8359567751516153 1103 74-75 0.631864978194745 1103 76-77 0.6118989068424021 1103 78-79 0.28843030041867834 1103 80-81 0.7379220649409 1103 82-83 0.7651511121057375 1103 84-85 0.8707180068587022 1103 86-87 0.9434470605552185 1103 88-89 0.7855163678517947 1103 90-91 0.8138751117163778 1103 92-93 1.0367405644845462 1103 94-95 1.0850678988073454 1103 96-97 1.0630912344672296 1103 98-99 0.820986526095453 1103 100-101 1.2990926462128947 1104 1 0.1240857401837836 1104 2 0.18841744751638956 1104 3 0.09408219586051203 1104 4 0.2096480327280119 1104 5 0.12851860224230194 1104 6 -0.12237715924649706 1104 7 0.07149389048687738 1104 8 0.11388846185793255 1104 9 0.16747991943918805 1104 10-11 0.14603478687892846 1104 12-13 0.11094321165294474 1104 14-15 0.1267838655226896 1104 16-17 0.187623930130826 1104 18-19 0.10435028539307467 1104 20-21 0.08608219819974039 1104 22-23 0.06330228497794366 1104 24-25 0.12023959812673723 1104 26-27 0.04065653681612247 1104 28-29 0.15395888387831036 1104 30-31 0.13340367998894465 1104 32-33 0.2729057620459159 1104 34-35 0.0630841091540475 1104 36-37 0.07260343612018971 1104 38-39 0.026402772220762927 1104 40-41 0.24641476740472257 1104 42-43 0.09169006955108472 1104 44-45 0.0909061367653905 1104 46-47 0.34765713944747745 1104 48-49 0.4148339764402209 1104 50-51 0.3558918641082869 1104 52-53 0.39330737204503663 1104 54-55 0.3805034559556617 1104 56-57 -0.03016921739823175 1104 58-59 0.1291420421794527 1104 60-61 0.4018921930347723 1104 62-63 0.1774121909416415 1104 64-65 0.2326468993973343 1104 66-67 0.22858116396588102 1104 68-69 0.1823409328662038 1104 70-71 0.3915926664226035 1104 72-73 0.5190142178287047 1104 74-75 0.49670510091852194 1104 76-77 0.3310230219621957 1104 78-79 -0.08841208515210397 1104 80-81 0.186934745038549 1104 82-83 0.19972858658500314 1104 84-85 0.6043442191891799 1104 86-87 0.5175170763034096 1104 88-89 0.5013188376167967 1104 90-91 0.6514190053421842 1104 92-93 0.4925449709204095 1104 94-95 0.5599230307303067 1104 96-97 0.9909935650977175 1104 98-99 1.0258882145666561 1104 100-101 1.698383213096843 1105 1 0.03428698850519751 1105 2 0.11562921799771786 1105 3 0.0022522595233454012 1105 4 0.0937464802483774 1105 5 0.13061731989464676 1105 6 0.17077382634560223 1105 7 0.04622356370511227 1105 8 0.275800762473267 1105 9 0.12331658829634762 1105 10-11 0.19989105069397084 1105 12-13 0.23994064539918014 1105 14-15 0.23567302569797022 1105 16-17 0.23344181962087873 1105 18-19 0.18876165742972972 1105 20-21 0.3147998895064248 1105 22-23 0.35168083078479384 1105 24-25 0.2844149033888499 1105 26-27 0.15042882075599806 1105 28-29 0.07188778981784338 1105 30-31 -0.019753682276217432 1105 32-33 -0.055592252224329286 1105 34-35 0.15808192731886805 1105 36-37 0.2635786255372494 1105 38-39 -0.05255823267017945 1105 40-41 0.13352200460396801 1105 42-43 0.29093155714709695 1105 44-45 0.24999211665613785 1105 46-47 0.02538806981836217 1105 48-49 0.07703004550049997 1105 50-51 0.30546289972674856 1105 52-53 0.3512969797562633 1105 54-55 0.22653634712104775 1105 56-57 0.14998413444606484 1105 58-59 0.13325928205405546 1105 60-61 0.21528521947393742 1105 62-63 0.07341690006924395 1105 64-65 0.2042353143719957 1105 66-67 0.23560199789445946 1105 68-69 0.1438562174008844 1105 70-71 0.1755140346410542 1105 72-73 0.2116835762912359 1105 74-75 0.046405488194267264 1105 76-77 -0.007721500722396968 1105 78-79 0.039689562393203204 1105 80-81 -0.09578109430565718 1105 82-83 0.21433064841925287 1105 84-85 0.37124857169062864 1105 86-87 0.5257177895557241 1105 88-89 0.5505510394261641 1105 90-91 0.4932275129409476 1105 92-93 0.5715420150532182 1105 94-95 0.6234663830161686 1105 96-97 0.7462530216649128 1105 98-99 0.7619475215222948 1105 100-101 1.1203024098698044 1106 1 0.12232879412560393 1106 2 0.04958720008009365 1106 3 0.1163763413867187 1106 4 0.2155529863115504 1106 5 0.21107373286614006 1106 6 0.006847486968304395 1106 7 -0.007695045709027681 1106 8 0.06916067854422892 1106 9 0.052697481416423386 1106 10-11 -0.0027634134411513855 1106 12-13 0.1879874133042705 1106 14-15 0.28249595874684985 1106 16-17 0.15737413067147088 1106 18-19 0.1402012735234237 1106 20-21 0.06661646340702276 1106 22-23 0.015862936213160594 1106 24-25 0.06213434284604347 1106 26-27 -0.08103815302529682 1106 28-29 -0.09050688373611138 1106 30-31 0.12093815919376993 1106 32-33 0.09259543935600334 1106 34-35 0.08535971041492729 1106 36-37 -0.1296132436095263 1106 38-39 0.2737655479395258 1106 40-41 0.3328046223458969 1106 42-43 0.31682253796676463 1106 44-45 0.1798518181506168 1106 46-47 0.10477501927013577 1106 48-49 0.03548198199628416 1106 50-51 0.08233966249372315 1106 52-53 -0.19907802439525568 1106 54-55 -0.15326630763613736 1106 56-57 -0.2913220339603271 1106 58-59 -0.141687106386037 1106 60-61 -0.1605204191662395 1106 62-63 0.012556790260788375 1106 64-65 0.08527398177063361 1106 66-67 0.047659372845860304 1106 68-69 0.15617710790306205 1106 70-71 0.30771235931644014 1106 72-73 0.3789881114417284 1106 74-75 0.28484294159144596 1106 76-77 0.31593570308879393 1106 78-79 0.4215020401296705 1106 80-81 0.7078234650050419 1106 82-83 0.5947312845294448 1106 84-85 0.7272879391491109 1106 86-87 0.815125949192808 1106 88-89 0.6695740749768291 1106 90-91 0.9253378254411473 1106 92-93 0.9136594714199546 1106 94-95 0.7935384338268392 1106 96-97 1.2671047112186535 1106 98-99 1.0983747937700343 1106 100-101 1.7485795853790407 1107 1 0.010639838452675576 1107 2 0.10741398103701982 1107 3 0.1417955989143742 1107 4 0.05147241909718758 1107 5 0.10973017743132374 1107 6 -0.0992676299600106 1107 7 -0.05475139658052797 1107 8 0.0804841152313216 1107 9 -0.023788510809410468 1107 10-11 0.00142055765414284 1107 12-13 -0.10723624695787493 1107 14-15 -0.01647862333219763 1107 16-17 0.03573255981194734 1107 18-19 -0.15125408948781427 1107 20-21 -0.06380621741528358 1107 22-23 0.014345927166907302 1107 24-25 0.07905133271248843 1107 26-27 -0.13034278481478623 1107 28-29 0.08084079168143177 1107 30-31 -0.10970051163899086 1107 32-33 -0.0036472566965315423 1107 34-35 0.01609722073577302 1107 36-37 -0.011656768403426554 1107 38-39 -0.20059165289141845 1107 40-41 0.0465199545959365 1107 42-43 0.12967290774606255 1107 44-45 0.2627759098915803 1107 46-47 0.13249702301855848 1107 48-49 0.023101149906800345 1107 50-51 0.2026774075165818 1107 52-53 0.27608429390198097 1107 54-55 0.040645373701075016 1107 56-57 0.10595540260180059 1107 58-59 0.11706150817370542 1107 60-61 0.22753684358139736 1107 62-63 0.3117590907682519 1107 64-65 0.19659554725713946 1107 66-67 0.2561004255164825 1107 68-69 0.39769213214011856 1107 70-71 0.3432170538579804 1107 72-73 0.25366411807246436 1107 74-75 0.44550556045027534 1107 76-77 0.3551682635828719 1107 78-79 0.5666478952031788 1107 80-81 0.6380331965178314 1107 82-83 0.3984100982951979 1107 84-85 0.8069671685887094 1107 86-87 0.9162008536863979 1107 88-89 0.8890107700890866 1107 90-91 0.80618378186022 1107 92-93 0.6274989377802811 1107 94-95 0.8573535578724005 1107 96-97 0.9121483185458743 1107 98-99 0.9504854579092452 1107 100-101 1.2975981907932912 1108 1 0.10772830723596272 1108 2 0.16902608819368226 1108 3 0.10432504740771265 1108 4 0.20403406635889354 1108 5 0.1498000667731958 1108 6 0.19327453363631975 1108 7 0.1560363276608996 1108 8 0.23752525654736445 1108 9 0.3692264027646175 1108 10-11 0.2005709019413544 1108 12-13 0.19988106254731974 1108 14-15 0.18879141082543782 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2316 86-87 -2.079131325441441 2316 88-89 -2.3590975310902955 2316 90-91 -2.0670527749066707 2316 92-93 -2.3070909969714677 2316 94-95 -2.567273676220495 2316 96-97 -2.7551996289097183 2316 98-99 -3.5742809519808247 2316 100-101 -3.651732320727529 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 13.0 4 43.0 5 97.0 6 243.0 7 556.0 8 1186.0 9 2145.0 10 3489.0 11 5248.0 12 7384.0 13 9793.0 14 12336.0 15 16180.0 16 20244.0 17 25337.0 18 21777.0 19 15484.0 20 14404.0 21 15064.0 22 16288.0 23 18077.0 24 20880.0 25 24990.0 26 29446.0 27 35402.0 28 43309.0 29 51369.0 30 60926.0 31 72326.0 32 87093.0 33 105532.0 34 131821.0 35 165011.0 36 202511.0 37 203506.0 38 83074.0 39 2769.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.91768192726844 20.045571977495968 15.469293011422502 20.567453083813096 2 16.197368714373198 22.671202069780342 37.767852576669334 23.363576639177122 3 20.892095425047437 26.42481271529709 30.06757066858639 22.615521191069078 4 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31.061957461649857 21.601163796183574 24-25 22.437498156166924 25.54798718986728 31.1462905356458 20.868224118319997 26-27 21.871438269567328 25.18911487649062 31.988465443040344 20.950981410901708 28-29 21.013357319777146 26.715950766600276 31.369377685526707 20.90131422809587 30-31 21.51021399582452 25.5079626848817 31.150958134729223 21.830865184564555 32-33 21.499395674623482 25.773993782676925 31.21872448171764 21.507886060981953 34-35 22.486821656637655 25.39456358302774 31.0225463499734 21.096068410361205 36-37 22.327083917218594 25.076564771518527 31.384754571514268 21.211596739748614 38-39 21.736617806473777 25.80807473226435 31.336683627929148 21.118623833332734 40-41 21.768935377232477 26.002022483327625 30.8093375137878 21.419704625652102 42-43 21.475180801565077 26.296686992842677 29.907473393601613 22.32065881199063 44-45 22.066332509577432 25.63241212314315 29.875394217843283 22.425861149436138 46-47 22.916629789956815 26.51799288427007 29.230381426463254 21.334995899309863 48-49 22.712361097453044 26.623168453141904 28.79434228209919 21.870128167305865 50-51 21.845624637174268 27.06061250719915 29.277737914522767 21.81602494110382 52-53 22.696728361715852 27.499849215131984 27.87441849487856 21.929003928273605 54-55 21.81112046426614 26.814359103460756 28.494616254035186 22.87990417823792 56-57 22.793523448469625 26.832828086955924 28.047386394966832 22.32626206960762 58-59 23.550534330321515 26.32307164688625 27.847177003435426 22.27921701935681 60-61 22.49473305282291 26.81828524067965 29.156986827550348 21.529994878947097 62-63 22.226028733294015 26.866905176458015 28.972415762365305 21.934650327882665 64-65 21.925499580192977 27.622089094819994 28.433901785810882 22.018509539176144 66-67 22.362068649021456 26.970794401714 27.803568291064717 22.86356865819983 68-69 22.234255898158388 27.009949854098963 28.14798449404038 22.60780975370227 70-71 22.815104826520543 26.990366495373042 27.93021420936196 22.26431446874446 72-73 22.622209256205934 26.577223007103264 28.577085334233672 22.223482402457133 74-75 21.95345492164526 28.37802887686897 27.548934220174736 22.119581981311036 76-77 22.30013252874215 27.07182284221212 27.73302424164746 22.89502038739826 78-79 22.357803665845704 27.17484411485001 27.97320948846461 22.494142730839673 80-81 22.79315462481801 27.32475208119351 27.59325087786649 22.288842416121994 82-83 22.59508710088596 27.10234813077984 28.03922031462277 22.263344453711426 84-85 22.080370111708948 27.608077668374925 27.807265180055673 22.504287039860454 86-87 22.20699290979162 27.704297529114175 27.28963114394028 22.799078417153925 88-89 21.937880426477715 27.764894223321697 26.90728890736892 23.38993644283167 90-91 22.295829556589506 27.098962275412187 27.208591693218654 23.39661647477966 92-93 22.66710077982987 27.644254099156168 27.2676838816799 22.420961239334062 94-95 22.225756094809938 27.29971221526049 27.458834847233422 23.015696842696144 96-97 21.495508855862624 27.36129827172365 28.01530094715933 23.127891925254403 98-99 21.883030847637723 27.87782289008848 26.74745341370467 23.491692848569123 100-101 21.68288631986608 27.38994993729042 27.064562003767822 23.862601739075675 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1565.0 1 1145.5 2 1318.5 3 1703.5 4 1194.5 5 951.0 6 1445.5 7 2674.0 8 3889.5 9 4393.0 10 3806.0 11 2907.0 12 2649.0 13 2698.0 14 2900.5 15 3057.5 16 3190.5 17 3457.5 18 3663.5 19 3883.5 20 4258.5 21 4637.0 22 5122.5 23 5834.5 24 6595.5 25 7585.0 26 9101.5 27 10804.5 28 12437.5 29 14725.5 30 17732.5 31 20528.5 32 23879.0 33 26726.0 34 28410.5 35 29786.5 36 32933.5 37 36255.0 38 37245.5 39 37582.0 40 38116.0 41 40298.0 42 42140.5 43 43677.5 44 46123.5 45 48441.0 46 50568.5 47 60417.0 48 81507.5 49 80269.0 50 60090.0 51 50699.0 52 47283.0 53 46843.5 54 46462.0 55 43719.0 56 39956.5 57 36477.5 58 34361.0 59 31551.5 60 26694.0 61 22754.5 62 19661.5 63 15162.5 64 10998.0 65 8499.0 66 6402.5 67 5047.0 68 3798.0 69 2700.0 70 2175.5 71 1759.5 72 1465.5 73 1330.0 74 1105.0 75 899.5 76 665.0 77 414.5 78 285.5 79 213.0 80 150.5 81 108.0 82 74.0 83 40.0 84 25.0 85 14.5 86 8.5 87 4.5 88 2.0 89 1.5 90 1.5 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01933976025253138 2 0.011079388076873907 3 0.008981515778294234 4 0.0 5 0.002228989317240905 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.001245611677281682 14-15 1.3111701866122966E-4 16-17 3.2779254665307416E-5 18-19 0.001475066459938834 20-21 0.0 22-23 1.3111701866122966E-4 24-25 0.0 26-27 0.0016717419879306785 28-29 0.003212366957200127 30-31 0.001409507950608219 32-33 0.006588630187726792 34-35 0.0019995345345837526 36-37 8.194813666326855E-4 38-39 0.001081715403955145 40-41 1.6389627332653712E-4 42-43 0.004195744597159349 44-45 0.0016061834786000634 46-47 1.3111701866122966E-4 48-49 3.2779254665307424E-4 50-51 9.833776399592225E-5 52-53 1.966755279918445E-4 54-55 0.0015078457146041413 56-57 0.012554454536812743 58-59 0.02373218037768257 60-61 0.018225265593910925 62-63 0.012488896027482127 64-65 0.016356848077988404 66-67 0.00203231378924906 68-69 3.93351055983689E-4 70-71 9.833776399592225E-5 72-73 0.0 74-75 0.001081715403955145 76-77 0.0016717419879306785 78-79 0.007440890809024785 80-81 0.013668949195433194 82-83 0.01488178161804957 84-85 0.026878988825552085 86-87 0.010030451927584071 88-89 0.0384172864677403 90-91 0.04412087677950379 92-93 0.02668231329756024 94-95 0.02822293826682969 96-97 0.04874275168731214 98-99 0.05015225963792035 100-101 0.05683922758964306 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1525355.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.367281837009834 #Duplication Level Percentage of deduplicated Percentage of total 1 76.23413303075353 38.39706063960064 2 13.19359554151852 13.290510901663596 3 4.395966214551423 6.642386078228543 4 1.9982956874741233 4.0259488833876205 5 1.1034530329951537 2.778896495338511 6 0.6476069801101771 1.9570921972094468 7 0.43642540728624346 1.5387093042732622 8 0.29869725944049574 1.203565524014552 9 0.21019309923194784 0.9528169562289085 >10 1.312646660256472 12.246189102178041 >50 0.10747792048842106 3.7291718069556343 >100 0.053576793470343755 5.001724889250985 >500 0.004031205657678912 1.3887340640269907 >1k 0.003250972304579767 3.6758257388729323 >5k 3.9011667654957204E-4 1.488093096952942 >10k+ 2.6007778436638136E-4 1.6832743218173685 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14233 0.9330942633026411 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11467 0.7517594264941604 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9851 0.6458168754158867 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6764 0.4434377571122788 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6105 0.4002346994634036 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4297 0.281704914593652 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4271 0.280000393351056 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3850 0.25240026092286716 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3721 0.24394321321921783 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3484 0.22840584650786208 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3107 0.20369028849022028 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2780 0.18225265593910925 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2584 0.16940318811030874 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2526 0.1656007945691331 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2334 0.15301356077765504 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2286 0.14986675232978552 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2266 0.14855558214317321 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2242 0.14698217791923848 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2025 0.13275598139449507 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1836 0.12036542313100884 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1543 0.1011567798971387 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.277925466530742E-5 0.0 12-13 0.0 0.0 0.0 6.555850933061485E-5 0.0 14-15 0.0 0.0 0.0 6.555850933061485E-5 0.0 16-17 0.0 0.0 0.0 1.311170186612297E-4 0.0 18-19 0.0 0.0 0.0 1.311170186612297E-4 0.0 20-21 0.0 0.0 0.0 1.311170186612297E-4 0.0 22-23 0.0 0.0 0.0 2.2945478265715195E-4 0.0 24-25 0.0 0.0 0.0 5.900265839755335E-4 0.0 26-27 0.0 0.0 0.0 8.52260621297993E-4 0.0 28-29 0.0 0.0 0.0 0.0016717419879306785 0.0 30-31 0.0 0.0 0.0 0.004556316398477731 0.0 32-33 0.0 0.0 0.0 0.009342087579612614 0.0 34-35 0.0 0.0 0.0 0.014390092798069958 0.0 36-37 0.0 0.0 0.0 0.021699866588433515 0.0 38-39 0.0 0.0 0.0 0.031041954168046126 0.0 40-41 0.0 0.0 0.0 0.04376030497818541 0.0 42-43 0.0 0.0 0.0 0.05680644833497776 0.0 44-45 0.0 0.0 0.0 0.07417945330759068 0.0 46-47 0.0 0.0 0.0 0.09755106188395488 0.0 48-49 0.0 0.0 0.0 0.12488896027482127 0.0 50-51 0.0 0.0 0.0 0.15484919903891226 0.0 52-53 0.0 0.0 0.0 0.19044746960543613 0.0 54-55 0.0 0.0 0.0 0.2320443437757112 0.0 56-57 0.0 0.0 0.0 0.2848845022961868 0.0 58-59 0.0 0.0 0.0 0.3467061765949566 0.0 60-61 0.0 0.0 0.0 0.4200989277905799 0.0 62-63 0.0 0.0 0.0 0.4938194715328563 0.0 64-65 0.0 0.0 0.0 0.5720307731642797 0.0 66-67 0.0 0.0 0.0 0.6669922739296754 0.0 68-69 0.0 0.0 0.0 0.7839814338301576 0.0 70-71 0.0 0.0 0.0 0.9169013114979792 0.0 72-73 0.0 0.0 0.0 1.0408396733875067 0.0 74-75 0.0 0.0 0.0 1.1865762396294635 0.0 76-77 0.0 0.0 0.0 1.3339189893500203 0.0 78-79 0.0 0.0 0.0 1.5026338131123573 0.0 80-81 0.0 0.0 3.277925466530742E-5 1.6977359368802674 0.0 82-83 0.0 0.0 6.555850933061485E-5 1.9169963713365086 0.0 84-85 0.0 0.0 6.555850933061485E-5 2.1340605957301744 0.0 86-87 0.0 0.0 6.555850933061485E-5 2.3649576655925997 0.0 88-89 0.0 0.0 6.555850933061485E-5 2.6339442293761124 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11800 0.0 23.595367 1 TACATGG 11925 0.0 23.103674 2 GAGTACT 10285 0.0 22.05004 12-13 AGTACTT 10465 0.0 22.01115 12-13 ACATGGG 12040 0.0 21.460566 3 CATGGGA 7580 0.0 20.990164 4 GTACTTT 10850 0.0 20.638493 14-15 GGTATAG 260 1.4864781E-8 20.101593 1 TAAGGTG 640 0.0 20.0366 5 ATATAGG 525 0.0 19.90558 2 TACTTTT 11660 0.0 19.693546 14-15 CGTATAG 145 6.136107E-4 19.660492 1 ATGGGAG 2860 0.0 19.097303 5 GTGTAGC 600 0.0 19.005142 1 AGAGTAC 18995 0.0 19.002666 10-11 TATCACG 125 0.006030597 19.00078 2 CCTATAC 360 9.640644E-11 18.471165 3 ACTTTTT 12265 0.0 18.41234 16-17 GTAAGCG 155 5.5551936E-9 18.3969 94-95 ATGGGAT 2900 0.0 18.1788 5 >>END_MODULE