FastQCFastQC Report
Thu 26 May 2016
SRR936415_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936415_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743635
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64340.865209410530704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49020.6591943628258488No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45800.6158935499270476No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38960.52391294116065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31490.4234604342184002No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28810.3874212483274725No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25020.3364553846981382No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT17780.23909579296294553No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC14650.19700525123212326No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14380.19337443772818655No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11710.15746972641147874No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC11440.153838912907542No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC10980.14765308249342757No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG9520.12801979465732516No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA8900.11968237105569265No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8120.10919335426654206No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC7960.107041761079024No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT7750.10421779502040653No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA7730.10394884587196676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG701.6994818E-830.54890394-95
ACCGTAA650.00583716129.2365048
TGTATCG1004.9396334E-528.509439
GTACATG58000.026.7818221
TACATGG59750.025.6033482
AATTCGC1151.3460522E-1024.79581578-79
ACATGGG60200.024.1492543
GAGTACT46850.023.98040412-13
CTGGTCG1601.8527444E-623.7578569
TCGCGGA1202.2919266E-1023.7530682-83
CCCTATA1852.5343252E-723.1126422
ATGGGAG14500.022.9355345
CGCGGAA1153.623427E-922.72032282-83
GTACTTT50700.022.34981214-15
AGTACTT48050.022.24457412-13
TAGGACA2806.548362E-1122.05794
CATGGGA39800.021.9641084
ATTCGCG1306.220944E-1021.93254580-81
CAATTCG1205.8953447E-921.78243378-79
ACTATAC1353.7891802E-421.1152533
Produced by FastQC (version 0.11.5)