##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936415_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 743635 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.737981671115534 33.0 31.0 34.0 30.0 34.0 2 31.794021260430185 33.0 31.0 34.0 30.0 34.0 3 31.795671263455862 33.0 31.0 34.0 30.0 34.0 4 35.44432147491713 37.0 35.0 37.0 33.0 37.0 5 35.46767836371338 37.0 35.0 37.0 33.0 37.0 6 35.47218460669549 37.0 35.0 37.0 33.0 37.0 7 35.42293598337894 37.0 35.0 37.0 33.0 37.0 8 35.39741405393775 37.0 35.0 37.0 33.0 37.0 9 36.936159540634854 39.0 37.0 39.0 33.0 39.0 10-11 36.83116717206694 39.0 37.0 39.0 33.0 39.0 12-13 36.74970314737741 39.0 37.0 39.0 32.5 39.0 14-15 37.83511063895594 40.0 37.5 41.0 33.0 41.0 16-17 37.78935028609466 40.0 37.5 41.0 33.0 41.0 18-19 37.834166627444915 40.0 38.0 41.0 33.0 41.0 20-21 37.740349095994674 40.0 37.5 41.0 32.5 41.0 22-23 37.811482111519766 40.0 38.0 41.0 33.0 41.0 24-25 37.82006562359222 40.0 37.5 41.0 33.0 41.0 26-27 37.718058590571985 40.0 37.0 41.0 33.0 41.0 28-29 37.58449508159245 40.0 37.0 41.0 32.0 41.0 30-31 37.3370706058752 39.5 37.0 41.0 32.0 41.0 32-33 37.05656941913708 39.0 36.0 41.0 31.5 41.0 34-35 36.816003146705036 39.0 35.0 41.0 31.0 41.0 36-37 36.58628292105671 39.0 35.0 41.0 30.0 41.0 38-39 36.364866500366446 39.0 35.0 40.5 30.0 41.0 40-41 36.130587586652055 39.0 35.0 40.0 30.0 41.0 42-43 35.82801105381 38.5 35.0 40.0 29.5 41.0 44-45 35.45266629462034 38.0 35.0 40.0 27.5 41.0 46-47 35.06270885582309 38.0 35.0 40.0 25.0 41.0 48-49 34.75492277797575 38.5 34.5 40.0 24.0 41.0 50-51 33.73771742857719 37.5 33.0 39.5 22.5 40.5 52-53 33.81944704055081 38.0 33.5 39.5 22.5 40.5 54-55 34.24555998574569 38.0 34.0 40.0 22.5 41.0 56-57 34.09492291245033 38.0 34.0 40.0 21.5 41.0 58-59 33.858122600469315 38.0 34.0 40.0 20.5 41.0 60-61 33.54898370840533 37.5 33.0 40.0 19.5 41.0 62-63 33.22173310831255 37.0 33.0 40.0 18.0 41.0 64-65 32.85766874878132 36.5 33.0 39.5 15.5 41.0 66-67 32.361631042110716 36.0 32.0 39.0 10.5 41.0 68-69 31.971947931444863 35.0 32.0 39.0 8.0 40.5 70-71 31.549571362294675 35.0 31.0 38.0 7.0 40.0 72-73 31.178379177956927 35.0 31.0 37.0 3.5 40.0 74-75 30.78182374417557 35.0 31.0 37.0 2.0 39.0 76-77 30.326727493999073 35.0 30.0 36.5 2.0 39.0 78-79 29.844814324231645 34.0 30.0 36.0 2.0 38.5 80-81 29.406680024474372 34.0 29.0 35.5 2.0 37.0 82-83 28.997274872753437 34.0 29.0 35.0 2.0 37.0 84-85 28.646571234543828 34.0 29.0 35.0 2.0 36.5 86-87 28.34386964034775 34.0 29.0 35.0 2.0 36.0 88-89 27.94088968378304 34.0 27.5 35.0 2.0 36.0 90-91 27.533530562708854 34.0 27.0 35.0 2.0 35.0 92-93 27.082504857893994 33.5 25.5 35.0 2.0 35.0 94-95 26.574543290727306 33.0 25.0 35.0 2.0 35.0 96-97 26.015775212301733 33.0 23.5 35.0 2.0 35.0 98-99 25.34335325798275 33.0 19.0 35.0 2.0 35.0 100-101 22.65095241617191 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2820366442022255 1101 2 0.3154188117253689 1101 3 0.4009049992105993 1101 4 0.3224757897533195 1101 5 0.34080172392339136 1101 6 0.2960825804635263 1101 7 0.37484993088592944 1101 8 0.4678466483326815 1101 9 0.39478837724697513 1101 10-11 0.5175093854568971 1101 12-13 0.527008981177687 1101 14-15 0.6520388281178384 1101 16-17 0.7249352844024131 1101 18-19 0.6389523255246203 1101 20-21 0.6075306940707748 1101 22-23 0.5256276116990932 1101 24-25 0.3488008987652478 1101 26-27 0.5890184030024699 1101 28-29 0.651631683500085 1101 30-31 0.49555479023267424 1101 32-33 0.4185064880244269 1101 34-35 0.5684350419861914 1101 36-37 0.30097224713090753 1101 38-39 0.4618635514605387 1101 40-41 0.49249590656848596 1101 42-43 0.7422951087471361 1101 44-45 0.5915644636583508 1101 46-47 0.7107896990478864 1101 48-49 0.9820935279179395 1101 50-51 0.8376938526837279 1101 52-53 1.0046007348773855 1101 54-55 0.7534349052837044 1101 56-57 0.8837630420693401 1101 58-59 1.0004417860412218 1101 60-61 0.9543945762705306 1101 62-63 1.0440350723105354 1101 64-65 1.0189646302389122 1101 66-67 1.07893645741121 1101 68-69 1.0398225887298018 1101 70-71 1.081851898779604 1101 72-73 0.9256680911821356 1101 74-75 0.9141339569127247 1101 76-77 0.8690571402603595 1101 78-79 0.8305064856936148 1101 80-81 0.6710895139822419 1101 82-83 0.7138421788441285 1101 84-85 0.7754180994454778 1101 86-87 0.9194904030206565 1101 88-89 0.7436744015687573 1101 90-91 0.6921705368961995 1101 92-93 0.678091724921341 1101 94-95 0.7345023576762806 1101 96-97 0.4540706581471241 1101 98-99 0.31155184837385264 1101 100-101 0.5749055103126501 1102 1 0.40205859207602757 1102 2 0.39890752602890345 1102 3 0.35835206243543993 1102 4 0.4566246861244423 1102 5 0.3879801751018448 1102 6 0.3519523896666712 1102 7 0.3331175453016897 1102 8 0.40688094188549684 1102 9 0.5260371613846431 1102 10-11 0.5352622224689938 1102 12-13 0.6124533009182969 1102 14-15 0.610110657523002 1102 16-17 0.4594183870336579 1102 18-19 0.4409351163962967 1102 20-21 0.3855940436156047 1102 22-23 0.33240302010413814 1102 24-25 0.4121591728642713 1102 26-27 0.27233203313091536 1102 28-29 0.12352069709280755 1102 30-31 0.37486567687689387 1102 32-33 0.20442168942110328 1102 34-35 0.2602442451287317 1102 36-37 0.07803173085705595 1102 38-39 0.08131111350069631 1102 40-41 0.15797389634277437 1102 42-43 0.2341576851282312 1102 44-45 0.25204457339771835 1102 46-47 0.41924387039094313 1102 48-49 0.6115605437553242 1102 50-51 0.7614342193129886 1102 52-53 0.8279280824269577 1102 54-55 0.7082656821885465 1102 56-57 0.645106102301277 1102 58-59 0.5683667143365199 1102 60-61 0.5388185692871161 1102 62-63 0.5041178756525966 1102 64-65 0.6302368586963283 1102 66-67 0.7051454610276195 1102 68-69 0.6499674945784157 1102 70-71 0.448629053038232 1102 72-73 0.28183768838876944 1102 74-75 0.4354311261358177 1102 76-77 0.5414083062411592 1102 78-79 0.5163446201243396 1102 80-81 0.7818831291462267 1102 82-83 0.6560853513095246 1102 84-85 0.37668658678803624 1102 86-87 0.39217353059267523 1102 88-89 0.4962111268462266 1102 90-91 0.7005127242013494 1102 92-93 0.6092871358204555 1102 94-95 0.43995216438534257 1102 96-97 0.49686626857236504 1102 98-99 0.4836511126212635 1102 100-101 0.18314419344022426 1103 1 0.11793398148412493 1103 2 0.180427208838303 1103 3 0.15137613493614666 1103 4 0.443240375730781 1103 5 0.3238408378036297 1103 6 0.3263987221503868 1103 7 0.30911527965456287 1103 8 0.321541378022566 1103 9 0.20252654116374202 1103 10-11 0.4186125481557639 1103 12-13 0.1420350058177675 1103 14-15 0.3436997257756218 1103 16-17 0.42616151929081525 1103 18-19 0.576406654204284 1103 20-21 0.515950883037867 1103 22-23 0.24149591163627804 1103 24-25 0.37539763222422096 1103 26-27 0.13553424213686327 1103 28-29 0.38317886758275677 1103 30-31 0.1920616553532497 1103 32-33 0.17838912074368096 1103 34-35 0.47024651077585844 1103 36-37 0.6080133224741644 1103 38-39 0.6339803290203747 1103 40-41 0.35267596374577437 1103 42-43 0.6110469516249637 1103 44-45 0.6998327353914888 1103 46-47 0.6590609193855599 1103 48-49 1.0162252279807618 1103 50-51 0.8869083059739893 1103 52-53 0.8611302914329073 1103 54-55 1.0476703834063343 1103 56-57 0.9179636497382759 1103 58-59 0.8342446525976328 1103 60-61 1.0642493254468377 1103 62-63 0.9640028940609824 1103 64-65 0.9438921971273615 1103 66-67 0.9739276756976878 1103 68-69 0.9899142518709674 1103 70-71 0.8344223127380346 1103 72-73 0.5593296170208859 1103 74-75 0.7204090360763473 1103 76-77 0.8236326053065781 1103 78-79 0.805755835077612 1103 80-81 0.7410034916497636 1103 82-83 0.6852903923743803 1103 84-85 0.8349270285892274 1103 86-87 0.9498401972593378 1103 88-89 0.854166946727279 1103 90-91 0.9730122993925114 1103 92-93 0.9219268979275057 1103 94-95 0.8770385421820031 1103 96-97 0.8421871408465051 1103 98-99 0.7221498381947562 1103 100-101 0.7129915454651474 1104 1 0.2624848215842235 1104 2 0.3074559612002865 1104 3 0.2751747151925201 1104 4 0.30549204827048726 1104 5 0.20725588300050646 1104 6 0.29050678608992087 1104 7 0.31622931877411986 1104 8 0.4805858599182855 1104 9 0.44868097658583395 1104 10-11 0.515940314868395 1104 12-13 0.4064890684294795 1104 14-15 0.09241021042911512 1104 16-17 0.012428819224417964 1104 18-19 0.2406743116272949 1104 20-21 0.001227426626044803 1104 22-23 0.16744325855420783 1104 24-25 0.3159892771861763 1104 26-27 0.002655431044473744 1104 28-29 0.07105870007342929 1104 30-31 0.2975989108560313 1104 32-33 0.3493009740708857 1104 34-35 0.42419804542679174 1104 36-37 0.4807739067139849 1104 38-39 0.01320381096716261 1104 40-41 0.0730081735409911 1104 42-43 0.3032128267079557 1104 44-45 0.3203232619223044 1104 46-47 0.2769622012404227 1104 48-49 0.6531194111174656 1104 50-51 0.6228857841416016 1104 52-53 0.6356285295208437 1104 54-55 0.5345071128889956 1104 56-57 0.5720211544622416 1104 58-59 0.657882362622658 1104 60-61 0.6397664083333154 1104 62-63 0.8311879203619839 1104 64-65 0.6447629391732264 1104 66-67 0.7341991122943838 1104 68-69 0.5316843351149281 1104 70-71 0.32046362829903785 1104 72-73 0.37801354531872633 1104 74-75 0.17665616339364476 1104 76-77 0.3299984701755818 1104 78-79 0.4514429807800795 1104 80-81 0.6427832781775216 1104 82-83 0.5652403438579583 1104 84-85 0.5047447089937016 1104 86-87 0.42825549780578953 1104 88-89 0.45313661119793736 1104 90-91 0.36830109976021674 1104 92-93 0.2571574785924362 1104 94-95 0.4453649810768532 1104 96-97 0.6504400390641649 1104 98-99 0.5916227966749155 1104 100-101 0.7363573081943251 1105 1 0.32353084134574317 1105 2 0.18799772696720396 1105 3 0.08999904836090522 1105 4 0.2860378046514853 1105 5 0.2787908327985775 1105 6 0.37755564716866274 1105 7 0.33961272101205253 1105 8 0.48095299641324374 1105 9 0.4519828106720638 1105 10-11 0.48726894991875014 1105 12-13 0.35551216610375036 1105 14-15 0.35849837107527094 1105 16-17 0.3565450578312195 1105 18-19 0.2588248216277762 1105 20-21 0.3559826423586401 1105 22-23 0.32861298735207356 1105 24-25 0.3409314132820782 1105 26-27 0.2302150109126302 1105 28-29 0.26503047845068295 1105 30-31 0.3793737763723328 1105 32-33 -1.7705360802011683E-4 1105 34-35 0.1882885046666658 1105 36-37 0.447942326590443 1105 38-39 0.34963013625009154 1105 40-41 0.2373592198799983 1105 42-43 0.34528028404829314 1105 44-45 0.656504164439589 1105 46-47 0.8439785787205878 1105 48-49 0.888780336620691 1105 50-51 0.8401531712471311 1105 52-53 0.8830262500820893 1105 54-55 0.7782538206323935 1105 56-57 0.8357337025491418 1105 58-59 1.000541628030419 1105 60-61 0.8430897917355509 1105 62-63 0.5879408375008808 1105 64-65 0.7864556778518548 1105 66-67 0.9843647227785297 1105 68-69 0.9368864996240056 1105 70-71 0.7526595036003272 1105 72-73 0.49623867980242053 1105 74-75 0.7732362071307719 1105 76-77 0.6848630287338544 1105 78-79 0.7277487097056472 1105 80-81 0.8613332486469751 1105 82-83 0.7330859709430548 1105 84-85 0.6045934647202316 1105 86-87 0.34780352085557453 1105 88-89 0.34833151278939667 1105 90-91 0.4021726339118885 1105 92-93 0.044754250983771726 1105 94-95 0.2841872710508291 1105 96-97 0.46870268532017434 1105 98-99 0.7502588845961249 1105 100-101 0.8036622070721613 1106 1 0.27606069512544806 1106 2 0.15544858328919986 1106 3 0.22492390069182022 1106 4 0.16214423492474594 1106 5 0.13090570803368706 1106 6 0.25073070913899187 1106 7 0.12956989211365766 1106 8 0.24550975366257433 1106 9 0.36550795767563216 1106 10-11 0.5304500630196074 1106 12-13 0.3179946449580697 1106 14-15 0.3205846083881241 1106 16-17 0.2389729229288875 1106 18-19 -0.16401660906016247 1106 20-21 -0.05554542679831087 1106 22-23 0.16765701111837927 1106 24-25 0.13177680024215022 1106 26-27 0.03338279309650716 1106 28-29 0.05675537828962973 1106 30-31 0.21025082170052656 1106 32-33 0.1751055628557694 1106 34-35 0.23522828107175542 1106 36-37 0.18982219066013784 1106 38-39 -0.06952532661019717 1106 40-41 0.17976034123331885 1106 42-43 0.2839785435547597 1106 44-45 0.05587212334513936 1106 46-47 0.26618622771815836 1106 48-49 0.5698222778151703 1106 50-51 0.260539849749847 1106 52-53 0.24241732819696438 1106 54-55 0.25566344530871277 1106 56-57 0.39008841756751167 1106 58-59 0.31935992722247875 1106 60-61 0.5649323251421947 1106 62-63 0.3123794185261346 1106 64-65 0.1674849229349249 1106 66-67 0.29294012692953686 1106 68-69 0.3699667298331697 1106 70-71 0.39570065231172435 1106 72-73 0.04864239157202377 1106 74-75 0.3803304665430467 1106 76-77 0.40015068268700205 1106 78-79 0.2561511395775504 1106 80-81 0.715734959755153 1106 82-83 0.4574129490535235 1106 84-85 0.4085030479294183 1106 86-87 0.6179527104033973 1106 88-89 0.46528215256078553 1106 90-91 0.45866930579010656 1106 92-93 0.6125120665154178 1106 94-95 0.7335103328204866 1106 96-97 0.4750870887616401 1106 98-99 0.2486529288912216 1106 100-101 0.13018520290394875 1107 1 0.2094841904110929 1107 2 0.31518186147492955 1107 3 0.2613213179457965 1107 4 0.27781851830682314 1107 5 0.30077782906079165 1107 6 0.3619154962790958 1107 7 0.3176571347445005 1107 8 0.357865333144602 1107 9 0.2456231986564248 1107 10-11 0.5589364254772207 1107 12-13 0.42224877911336733 1107 14-15 0.28293706940456786 1107 16-17 0.2911864316841175 1107 18-19 0.22280059742931968 1107 20-21 0.27804887286544044 1107 22-23 0.19239847937950572 1107 24-25 0.29647664519423245 1107 26-27 0.32699781687890095 1107 28-29 0.12146299205070932 1107 30-31 0.08521512347608251 1107 32-33 0.11517412533067528 1107 34-35 0.4406328554778014 1107 36-37 0.5215886318125058 1107 38-39 0.3783596424951128 1107 40-41 0.2695043097379184 1107 42-43 0.07574656035290417 1107 44-45 0.2029706920241452 1107 46-47 0.45703632143625583 1107 48-49 0.5846601559017657 1107 50-51 0.43758444161929333 1107 52-53 0.6985355935422746 1107 54-55 0.7254455138033435 1107 56-57 0.548555161046913 1107 58-59 0.43309474385122826 1107 60-61 0.559245775795489 1107 62-63 0.7695103645372825 1107 64-65 0.73889942609771 1107 66-67 0.5961321226490313 1107 68-69 0.8280047663597081 1107 70-71 0.7441995761675528 1107 72-73 0.4948718384218793 1107 74-75 0.4549668370430169 1107 76-77 0.5464072656584307 1107 78-79 0.43998249733558126 1107 80-81 0.5298032880715873 1107 82-83 0.6217998532487918 1107 84-85 0.4240001805823823 1107 86-87 0.4014097428715502 1107 88-89 0.33316808456072877 1107 90-91 0.4069109835902438 1107 92-93 0.5874439800168396 1107 94-95 0.7598870806657345 1107 96-97 0.34615659115301156 1107 98-99 0.5669000010897172 1107 100-101 0.40894648272222156 1108 1 0.12048283450026886 1108 2 0.06591762703900272 1108 3 -0.0017792506958720367 1108 4 0.19990751440726484 1108 5 0.04071754883921841 1108 6 0.0636190563333372 1108 7 0.03303673722088263 1108 8 0.0042445782491284945 1108 9 0.08675433310181546 1108 10-11 0.24551474772151494 1108 12-13 0.09275128071628558 1108 14-15 0.3362419706543136 1108 16-17 0.24760020521548398 1108 18-19 0.3016674396286234 1108 20-21 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2316 60-61 -2.720734256265711 2316 62-63 -2.41969188215716 2316 64-65 -2.798152751693287 2316 66-67 -2.7313129454307905 2316 68-69 -2.6816899290790097 2316 70-71 -2.2932824948733135 2316 72-73 -2.2148467622956787 2316 74-75 -2.1011683824636904 2316 76-77 -1.870653469820617 2316 78-79 -3.0494700516903315 2316 80-81 -2.8656191523560572 2316 82-83 -2.556781619557448 2316 84-85 -2.7371645890631378 2316 86-87 -2.8553399829208423 2316 88-89 -2.876825728190628 2316 90-91 -2.95545989777127 2316 92-93 -3.294600544067226 2316 94-95 -3.563344430911247 2316 96-97 -4.056446472740376 2316 98-99 -5.030930845960693 2316 100-101 -5.84417626988024 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1592.0 3 575.0 4 580.0 5 577.0 6 631.0 7 837.0 8 998.0 9 1203.0 10 1524.0 11 2070.0 12 3042.0 13 3903.0 14 4485.0 15 6285.0 16 8303.0 17 11960.0 18 8845.0 19 5276.0 20 5002.0 21 5187.0 22 5445.0 23 5890.0 24 6807.0 25 7713.0 26 8711.0 27 10171.0 28 12135.0 29 14815.0 30 18244.0 31 23104.0 32 28764.0 33 38438.0 34 52691.0 35 74343.0 36 104075.0 37 135705.0 38 107075.0 39 16631.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.99959653015937 18.388137986685496 14.731759800954878 20.88050568220026 2 15.455988164884674 20.73175980095488 39.88810436419878 23.92414766996167 3 19.91185315494155 26.352329153284593 30.17597090643039 23.55984678534347 4 12.01446696445264 16.43633311678617 38.20860351290607 33.34059640585511 5 12.707400083403957 38.53854742591173 33.47700337651506 15.277049114169255 6 30.007776614723902 36.22274484900182 17.7332378971388 16.036240639135478 7 27.317632091749118 34.62593112092233 20.418831269256167 17.637605518072384 8 27.010129000147963 32.05512435937101 19.894674540294048 21.040072100186975 9 27.423520865283717 16.605187397422444 21.0520085021659 34.919283235127935 10-11 25.014025349221104 26.499905152314618 26.296554405574085 22.18951509289019 12-13 27.338514674675213 24.628971343438185 27.050368422291648 20.982145559594954 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25.59501637227941 31.55123145091342 21.399611368480503 40-41 21.23892079723193 26.030461672110732 31.427349611294968 21.303267919362366 42-43 21.043210939096944 26.21529085216942 30.362637303525513 22.37886090520812 44-45 21.239452757025276 25.785870039543124 30.51463889077531 22.460038312656287 46-47 22.150255235144453 26.445413566998553 29.87589224719887 21.528438950658124 48-49 21.993292354117443 26.49802522494416 29.37519919207024 22.133483228868158 50-51 21.220271515118913 26.938361092246556 29.87990936048036 21.96145803215417 52-53 21.935915073419157 27.33392773333226 28.57631786683019 22.15383932641839 54-55 21.171035175568537 26.762560709845435 29.18073395790874 22.885670156677293 56-57 21.969250754409465 26.690697499287282 28.592791019165496 22.747260727137764 58-59 22.703414367806044 26.260400514797745 28.686727820191958 22.34945729720425 60-61 21.881261615506887 26.53936564203664 29.88590916303801 21.693463579418466 62-63 21.518842653355424 26.83935064655636 29.588896120661833 22.05291057942638 64-65 21.098774455255548 27.427068758123173 29.311141248620782 22.163015538000494 66-67 21.561145796066807 26.975540605607396 28.450017349949835 23.013296248375966 68-69 21.33160774068217 26.82823469584748 29.147963964351803 22.69219359911855 70-71 22.001630667308888 26.7911777131371 28.575157348631823 22.632034270922187 72-73 21.763573359048692 26.535911056058005 29.317796521836197 22.382719063057102 74-75 21.213393004870845 27.907339626017112 28.55348982987693 22.325777539235112 76-77 21.449646735201046 26.99655982970653 28.592960261493296 22.96083317359913 78-79 21.51535436646629 27.068222566831608 28.762332773810286 22.654090292891816 80-81 21.88397731685012 27.32724689896685 28.214102340655046 22.57467344352799 82-83 21.719680136480843 26.97857866989033 28.80164196785892 22.500099225769908 84-85 21.48491273941935 27.323647950245565 28.293396763782773 22.898042546552315 86-87 21.461274425411037 27.45881574416803 28.201934664715505 22.87797516570543 88-89 21.262328212060382 27.3364336706043 27.679622848342394 23.72161526899293 90-91 21.323400820609404 26.919082531781797 28.212753077285264 23.544763570323536 92-93 21.93705541697743 27.3595812033812 28.175828403142855 22.527534976498508 94-95 21.54256356459346 27.009690196157543 28.113942181605438 23.333804057643555 96-97 20.89256231694974 27.123820155956302 28.711292540796464 23.2723249862975 98-99 21.32495258752051 27.61912322400824 27.45550896346464 23.60041522500661 100-101 21.266897688658617 27.023517141607513 27.780775830165194 23.928809339568684 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 217.0 1 191.0 2 266.0 3 386.5 4 328.0 5 302.5 6 566.5 7 1243.5 8 2086.0 9 2404.0 10 1996.0 11 1610.0 12 1584.0 13 1608.0 14 1627.5 15 1726.5 16 1848.5 17 1861.0 18 1933.5 19 2019.0 20 2103.5 21 2390.0 22 2710.5 23 3116.0 24 3594.5 25 4164.0 26 4954.0 27 5793.5 28 6622.0 29 7664.5 30 9075.0 31 10673.5 32 12642.0 33 13950.5 34 14763.5 35 15716.5 36 17150.0 37 18740.0 38 19133.0 39 18976.0 40 19218.5 41 20209.0 42 20791.5 43 21459.5 44 22582.0 45 23758.0 46 24745.5 47 28738.5 48 38284.0 49 37904.0 50 28471.0 51 23874.5 52 22040.0 53 21674.0 54 21481.5 55 20311.0 56 18807.5 57 17166.0 58 16024.0 59 14540.0 60 12482.0 61 10681.0 62 9177.0 63 7280.5 64 5318.5 65 4145.5 66 3078.0 67 2376.5 68 1740.0 69 1139.5 70 860.0 71 673.0 72 546.5 73 575.5 74 500.0 75 395.0 76 311.0 77 191.5 78 135.0 79 94.5 80 68.0 81 48.0 82 29.5 83 20.5 84 14.0 85 9.5 86 7.0 87 6.0 88 6.5 89 4.5 90 3.0 91 2.5 92 1.5 93 1.0 94 1.0 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011430338808689747 2 0.011430338808689747 3 0.013850881144647576 4 0.019902236984542146 5 0.03563576216826803 6 0.051234812777774044 7 0.005647932117234933 8 0.03025677919947286 9 0.039669999394864416 10-11 0.04545240608631923 12-13 0.03529957573271834 14-15 0.03200494866433129 16-17 0.0309963893576822 18-19 0.026289779259986417 20-21 0.054731151707490905 22-23 0.013312982847768058 24-25 0.0038997626523765015 26-27 0.0010085593066490952 28-29 1.3447457421987937E-4 30-31 0.0024877796230677682 32-33 0.0019498813261882508 34-35 0.0 36-37 4.034237226596381E-4 38-39 0.0 40-41 0.0016136948906385524 42-43 0.003630813503936743 44-45 0.0022188304746280094 46-47 9.413220195391555E-4 48-49 8.068474453192762E-4 50-51 0.004034237226596381 52-53 0.003765288078156622 54-55 0.004908321959025597 56-57 9.413220195391555E-4 58-59 0.005647932117234933 60-61 0.0022860677617379493 62-63 4.034237226596381E-4 64-65 8.740847324292159E-4 66-67 0.015733525183725887 68-69 0.04242672816637195 70-71 0.051234812777774044 72-73 0.014859440451296672 74-75 0.031198101219012012 76-77 0.028575847021724367 78-79 0.059504999092296625 80-81 0.08290357500655564 82-83 0.051167575490664106 84-85 0.019297101400552692 86-87 0.019767762410322267 88-89 0.022524491181829795 90-91 0.03832525365266562 92-93 0.023062389478709314 94-95 0.013447457421987937 96-97 0.021179745439631 98-99 0.05694998218211891 100-101 0.07530576156313244 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 743635.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.765700598138714 #Duplication Level Percentage of deduplicated Percentage of total 1 80.93353259754316 45.94248679781835 2 11.031400440319855 12.524103491467448 3 3.3634199641355362 5.7278067200976075 4 1.4482881836526944 3.2885237365220394 5 0.7863512291908366 2.2318889220612688 6 0.48938890475485425 1.6668302426019046 7 0.31537083685114004 1.2531572551453385 8 0.23730234131038416 1.0776506926450073 9 0.1731576527600789 0.8846473925569596 >10 1.08954423002371 11.650890040339979 >50 0.08380987218247059 3.2018249502398977 >100 0.042260818701344384 4.391992742279034 >500 0.0030864642869572446 1.247327016942716 >1k 0.0028490439571913027 4.043738499875661 >5k 2.374203297659419E-4 0.8671314994067459 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6434 0.865209410530704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4902 0.6591943628258488 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4580 0.6158935499270476 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3896 0.52391294116065 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3149 0.4234604342184002 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2881 0.3874212483274725 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2502 0.3364553846981382 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1778 0.23909579296294553 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1465 0.19700525123212326 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1438 0.19337443772818655 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1171 0.15746972641147874 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1144 0.153838912907542 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1098 0.14765308249342757 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 952 0.12801979465732516 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 890 0.11968237105569265 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 812 0.10919335426654206 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 796 0.107041761079024 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 775 0.10421779502040653 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 773 0.10394884587196676 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3447457421987937E-4 2 0.0 0.0 0.0 0.0 1.3447457421987937E-4 3 0.0 0.0 0.0 0.0 1.3447457421987937E-4 4 0.0 0.0 0.0 0.0 1.3447457421987937E-4 5 0.0 0.0 0.0 0.0 1.3447457421987937E-4 6 0.0 0.0 0.0 0.0 1.3447457421987937E-4 7 0.0 0.0 0.0 0.0 1.3447457421987937E-4 8 0.0 0.0 0.0 0.0 1.3447457421987937E-4 9 0.0 0.0 0.0 0.0 1.3447457421987937E-4 10-11 0.0 0.0 0.0 6.723728710993968E-5 1.3447457421987937E-4 12-13 0.0 0.0 0.0 1.3447457421987937E-4 1.3447457421987937E-4 14-15 0.0 0.0 0.0 2.0171186132981905E-4 1.3447457421987937E-4 16-17 0.0 0.0 0.0 2.6894914843975874E-4 1.3447457421987937E-4 18-19 0.0 0.0 0.0 2.6894914843975874E-4 1.3447457421987937E-4 20-21 0.0 0.0 0.0 2.6894914843975874E-4 1.3447457421987937E-4 22-23 0.0 0.0 0.0 4.034237226596381E-4 1.3447457421987937E-4 24-25 0.0 0.0 0.0 4.7066100976957776E-4 1.3447457421987937E-4 26-27 0.0 0.0 0.0 6.051355839894572E-4 2.0171186132981905E-4 28-29 0.0 0.0 0.0 0.0017481694648584319 2.6894914843975874E-4 30-31 0.0 0.0 0.0 0.003429101642606924 2.6894914843975874E-4 32-33 0.0 0.0 0.0 0.005513457543015054 2.6894914843975874E-4 34-35 0.0 0.0 0.0 0.008202949027412643 2.6894914843975874E-4 36-37 0.0 0.0 0.0 0.01250613540244878 3.3618643554969845E-4 38-39 0.0 0.0 0.0 0.016069711619275583 4.034237226596381E-4 40-41 0.0 0.0 0.0 0.022524491181829795 4.034237226596381E-4 42-43 0.0 0.0 0.0 0.036442609613587314 4.034237226596381E-4 44-45 0.0 0.0 0.0 0.059504999092296625 4.034237226596381E-4 46-47 0.0 0.0 0.0 0.08135711740302702 4.034237226596381E-4 48-49 0.0 0.0 0.0 0.1251958285987077 4.034237226596381E-4 50-51 0.0 0.0 0.0 0.17649787866359168 4.034237226596381E-4 52-53 0.0 0.0 0.0 0.23082560664842294 6.723728710993969E-4 54-55 0.0 0.0 0.0 0.3036435885884876 8.068474453192762E-4 56-57 0.0 1.3447457421987937E-4 0.0 0.39300194315759746 8.740847324292159E-4 58-59 0.0 1.3447457421987937E-4 0.0 0.49903514492997236 9.413220195391556E-4 60-61 0.0 1.3447457421987937E-4 0.0 0.6218104311927223 9.413220195391556E-4 62-63 0.0 1.3447457421987937E-4 0.0 0.7788767338815413 9.413220195391556E-4 64-65 0.0 1.3447457421987937E-4 0.0 0.9187302910702159 0.001075796593759035 66-67 0.0 1.3447457421987937E-4 0.0 1.0938161867044989 0.001075796593759035 68-69 0.0 1.3447457421987937E-4 0.0 1.3225574374525135 0.001075796593759035 70-71 0.0 1.3447457421987937E-4 0.0 1.5198988751201865 0.0011430338808689747 72-73 0.0 1.3447457421987937E-4 0.0 1.7576499223409334 0.001277508455088854 74-75 0.0 1.3447457421987937E-4 0.0 2.0294230368393094 0.0013447457421987938 76-77 0.0 1.3447457421987937E-4 0.0 2.2748391347905894 0.0013447457421987938 78-79 0.0 1.3447457421987937E-4 0.0 2.5360559952127053 0.0014792203164186732 80-81 0.0 1.3447457421987937E-4 0.0 2.8643756681705406 0.0014792203164186732 82-83 0.0 1.3447457421987937E-4 0.0 3.2671942552461894 0.0014792203164186732 84-85 0.0 1.3447457421987937E-4 0.0 3.70100923167952 0.0014792203164186732 86-87 0.0 1.3447457421987937E-4 0.0 4.163534529708795 0.0014792203164186732 88-89 0.0 1.3447457421987937E-4 0.0 4.664250606816516 0.0014792203164186732 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 70 1.6994818E-8 30.548903 94-95 ACCGTAA 65 0.005837161 29.236504 8 TGTATCG 100 4.9396334E-5 28.50943 9 GTACATG 5800 0.0 26.781822 1 TACATGG 5975 0.0 25.603348 2 AATTCGC 115 1.3460522E-10 24.795815 78-79 ACATGGG 6020 0.0 24.149254 3 GAGTACT 4685 0.0 23.980404 12-13 CTGGTCG 160 1.8527444E-6 23.757856 9 TCGCGGA 120 2.2919266E-10 23.75306 82-83 CCCTATA 185 2.5343252E-7 23.112642 2 ATGGGAG 1450 0.0 22.935534 5 CGCGGAA 115 3.623427E-9 22.720322 82-83 GTACTTT 5070 0.0 22.349812 14-15 AGTACTT 4805 0.0 22.244574 12-13 TAGGACA 280 6.548362E-11 22.0579 4 CATGGGA 3980 0.0 21.964108 4 ATTCGCG 130 6.220944E-10 21.932545 80-81 CAATTCG 120 5.8953447E-9 21.782433 78-79 ACTATAC 135 3.7891802E-4 21.115253 3 >>END_MODULE