FastQCFastQC Report
Thu 26 May 2016
SRR936415_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936415_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743635
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT79561.0698797124933603No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63090.848400088753219No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55910.7518473444633457No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37130.49930409407841214No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23220.3122499613385599No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21700.2918098260571383No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC18650.250795080920075No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16300.21919355597840337No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15120.20332555622045762No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA14680.1974086749547829No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14530.19539155634148475No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG14070.18920572592737028No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC13550.18221304806793656No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC13120.17643064137648173No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA12570.16903453979438837No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11300.1519562688684637No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10430.14025698091133418No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC8130.10932782884076192No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC8070.10852098139544267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAAG2750.038.0112462
TAAGGTG4150.030.900295
GTAAGGT3700.029.5238514
GTAAGCG1150.028.90798894-95
GGTAAGG4000.027.3150733
AAGGTAA3950.026.4653071
GTATAGG2750.025.9185031
GTACATG59300.024.4397371
GAGTACT55650.023.81145312-13
TGCGTAA1002.0552761E-823.7498460-61
TACATGG61500.023.1775912
GGTGTGC7850.022.9911588
AGTACTT56800.022.7858412-13
GGGCTAT2103.4458935E-822.6166156
ACATGGG61100.022.3138853
GTACTTT59450.022.09268814-15
AGGTGTG6150.021.623697
GGCGTTA550.002876188121.59294130-31
GCACGGT550.002877310821.59148866-67
CATGGGA41250.020.8401834
Produced by FastQC (version 0.11.5)