FastQCFastQC Report
Thu 26 May 2016
SRR936414_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936414_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852340
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74000.8681981368937278No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57400.6734401764554051No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51700.6065654551000774No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT41860.4911185677077223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35690.41872961494239386No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT31050.3642912452777061No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27170.318769505127062No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT17890.20989276579768634No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15770.1850200624164066No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC15130.17751132177300138No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC12940.15181734988384915No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12920.15158270173874278No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC12800.15017481286810427No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG11340.13304549827533613No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA10170.11931858178661098No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9320.10934603561958843No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT8980.10535701715277941No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA8840.1037144801370345No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAC250.005010158857.030657
AGGTAAG2053.092282E-1127.8165632
GTACATG67600.026.7139721
TACATGG68050.025.7676222
GTATTAG2251.0186341E-1025.3454691
ACATGGG68150.024.6141553
GAGTACT52250.024.2395912-13
GTCTAGG1801.9408071E-723.7613741
CTACGCA1252.2488297E-422.8095824
GTACTTT56600.022.37402514-15
CATGGGT7700.022.2171254
CATGGGA44850.021.9322874
GGTAAGG2858.367351E-1121.6757723
TTACGTG1100.002867801621.6025187
ACTAGTA1100.002869756721.5999832
AGTACTT53850.021.57781612-13
GGGATAC1805.142161E-621.1224637
ATGGGAT16200.020.8278715
GATCGCG804.9929993E-520.78022484-85
CGCGTGC1150.003710921420.6632799
Produced by FastQC (version 0.11.5)