##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936414_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 852340 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.631276251261234 31.0 31.0 34.0 30.0 34.0 2 31.675957951052396 33.0 31.0 34.0 30.0 34.0 3 31.690317244292185 33.0 31.0 34.0 30.0 34.0 4 35.359893939038415 37.0 35.0 37.0 33.0 37.0 5 35.32095173287655 37.0 35.0 37.0 33.0 37.0 6 35.44446699673839 37.0 35.0 37.0 33.0 37.0 7 35.310454748105215 37.0 35.0 37.0 33.0 37.0 8 35.30119670554004 37.0 35.0 37.0 33.0 37.0 9 36.84992725907502 39.0 37.0 39.0 33.0 39.0 10-11 36.72442569866485 39.0 37.0 39.0 32.0 39.0 12-13 36.64246016847737 39.0 37.0 39.0 32.0 39.0 14-15 37.69705340591783 40.0 37.0 41.0 32.0 41.0 16-17 37.67378862895089 40.0 37.0 41.0 32.0 41.0 18-19 37.70729286434991 40.0 37.0 41.0 32.0 41.0 20-21 37.63479304033602 40.0 37.0 41.0 32.0 41.0 22-23 37.52018619330314 40.0 37.0 41.0 32.0 41.0 24-25 37.36658962385903 39.5 37.0 41.0 32.0 41.0 26-27 37.175756153647605 39.0 36.5 41.0 31.5 41.0 28-29 37.251700025811296 39.0 37.0 41.0 31.0 41.0 30-31 37.12697045779853 39.0 36.0 41.0 31.0 41.0 32-33 36.884916817232565 39.0 35.5 41.0 31.0 41.0 34-35 36.668229227772954 39.0 35.0 41.0 30.5 41.0 36-37 36.409238684093204 39.0 35.0 40.5 30.0 41.0 38-39 36.20781495647277 39.0 35.0 40.0 30.0 41.0 40-41 36.031174179318114 38.5 35.0 40.0 30.0 41.0 42-43 35.69774679118662 38.0 35.0 40.0 28.5 41.0 44-45 35.313685266442974 38.0 35.0 40.0 26.5 41.0 46-47 34.80156744960931 38.0 34.0 40.0 24.5 41.0 48-49 34.40329563319802 38.0 34.0 40.0 23.0 41.0 50-51 33.64230236759978 37.5 33.0 39.5 22.5 40.5 52-53 33.80836755285449 38.0 33.5 39.5 22.5 40.5 54-55 34.156368350658184 38.0 34.0 40.0 22.0 41.0 56-57 34.04343923786283 38.0 34.0 40.0 21.5 41.0 58-59 33.79784417016684 38.0 34.0 40.0 20.5 41.0 60-61 33.45590668043269 37.0 33.0 40.0 19.5 41.0 62-63 33.16279125701011 37.0 33.0 40.0 18.0 41.0 64-65 32.847827158176315 36.0 33.0 39.5 17.0 41.0 66-67 32.451338667667834 36.0 32.0 39.0 13.0 41.0 68-69 32.06256247506863 35.0 32.0 39.0 9.5 40.5 70-71 31.634191167843817 35.0 31.0 38.0 7.5 40.0 72-73 31.241837764272475 35.0 31.0 37.0 6.5 40.0 74-75 30.832678273928245 35.0 31.0 37.0 2.0 39.0 76-77 30.442220240748995 35.0 30.5 36.5 2.0 39.0 78-79 29.990906797756764 34.5 30.0 36.0 2.0 38.5 80-81 29.584446934321985 34.0 29.5 35.5 2.0 37.0 82-83 29.188316868855154 34.0 29.0 35.0 2.0 37.0 84-85 28.805858577562944 34.0 29.0 35.0 2.0 36.5 86-87 28.520013140296125 34.0 29.0 35.0 2.0 36.0 88-89 28.125703357814956 34.0 28.0 35.0 2.0 36.0 90-91 27.76483093601145 34.0 27.0 35.0 2.0 35.0 92-93 27.39770572776122 34.0 26.5 35.0 2.0 35.0 94-95 26.973060633080696 33.5 25.5 35.0 2.0 35.0 96-97 26.40811002651524 33.0 25.0 35.0 2.0 35.0 98-99 25.79961224394021 33.0 23.5 35.0 2.0 35.0 100-101 23.85219747987892 31.0 10.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28270668141477273 1101 2 0.35683324447129294 1101 3 0.2230553080762121 1101 4 0.3704943271023424 1101 5 0.369871141000246 1101 6 0.11423748984704218 1101 7 0.1412499752275238 1101 8 0.29081853361998355 1101 9 0.45129106800373364 1101 10-11 0.3607820016477561 1101 12-13 0.5002839170944213 1101 14-15 0.4428121798405371 1101 16-17 0.6753553223142319 1101 18-19 0.5520539601476813 1101 20-21 0.4536861469146274 1101 22-23 0.41707187295317283 1101 24-25 0.4970894067437399 1101 26-27 0.6182179054638581 1101 28-29 0.5679167614124552 1101 30-31 0.40891748276769135 1101 32-33 0.28737585824188727 1101 34-35 0.248440678970411 1101 36-37 0.40186767579681515 1101 38-39 0.44747501729002437 1101 40-41 0.33728766642462205 1101 42-43 0.27614653176974713 1101 44-45 0.4957820749274475 1101 46-47 0.5686127708001933 1101 48-49 0.4541904487057735 1101 50-51 0.6946478747247724 1101 52-53 0.7400775444170478 1101 54-55 0.664926947681046 1101 56-57 0.6618938858785697 1101 58-59 0.7290505747846439 1101 60-61 0.6124531329436351 1101 62-63 0.5877767420360982 1101 64-65 0.7036452862600271 1101 66-67 0.4146104082758839 1101 68-69 0.37222832103345027 1101 70-71 0.367608829147553 1101 72-73 0.2695798784766126 1101 74-75 0.42035749994493443 1101 76-77 0.5400629711544553 1101 78-79 0.6488891676406894 1101 80-81 0.5771688410349078 1101 82-83 0.6686623595301526 1101 84-85 0.6444671114931566 1101 86-87 0.7610550924312633 1101 88-89 0.7822739328551087 1101 90-91 0.621753061003119 1101 92-93 0.3121512576347989 1101 94-95 0.11118593141192434 1101 96-97 -0.029732595353991087 1101 98-99 -0.2089870488251684 1101 100-101 -0.035604938049527135 1102 1 0.12331959256101399 1102 2 0.24071735081570367 1102 3 0.15881453060640283 1102 4 0.24398822161241185 1102 5 0.24734715023826226 1102 6 0.3470334916118958 1102 7 0.16470890335051536 1102 8 0.1975065015953419 1102 9 0.3513412462833898 1102 10-11 0.4077040510647407 1102 12-13 0.47478151696994075 1102 14-15 0.46230646478142035 1102 16-17 0.25128083668818135 1102 18-19 0.30672550207449234 1102 20-21 0.2473865597556042 1102 22-23 -0.0932177027771246 1102 24-25 0.05675543465068955 1102 26-27 0.2236370198940989 1102 28-29 0.21243346817049513 1102 30-31 0.24712957056151197 1102 32-33 0.29380352347961747 1102 34-35 0.18228245758309214 1102 36-37 -0.02150678322588817 1102 38-39 0.06923515206786135 1102 40-41 -0.26079246693684865 1102 42-43 -0.016438264630828314 1102 44-45 -0.006823980850107603 1102 46-47 0.030893334008275986 1102 48-49 0.23692502051545716 1102 50-51 0.23495706051811993 1102 52-53 0.06037267842846461 1102 54-55 -0.003976542761087387 1102 56-57 0.03497405418313804 1102 58-59 0.02158016110036698 1102 60-61 -0.37433302046026284 1102 62-63 -0.16529626992328872 1102 64-65 0.21950036467931255 1102 66-67 -0.0013172120017301836 1102 68-69 0.11713443360518383 1102 70-71 0.30146738044424737 1102 72-73 -0.0020125061657623178 1102 74-75 0.056561012238145025 1102 76-77 0.15924407281229236 1102 78-79 0.14359785174442408 1102 80-81 0.10721407603185895 1102 82-83 0.3264117821538015 1102 84-85 0.3533792771401849 1102 86-87 0.27493146004563584 1102 88-89 -0.12826942290237398 1102 90-91 -0.342264173861512 1102 92-93 -0.16160415097340675 1102 94-95 -0.21948963036816238 1102 96-97 -0.48887806437647185 1102 98-99 -0.63035693915511 1102 100-101 -0.4177586624938989 1103 1 0.1995765444091191 1103 2 0.36373203056748693 1103 3 0.1773806350504188 1103 4 0.21203933497119465 1103 5 0.29787277222175135 1103 6 0.05538542901717136 1103 7 0.08349795291783835 1103 8 0.09193712587205738 1103 9 0.3153845110018665 1103 10-11 0.3034521473745997 1103 12-13 0.40547717257743443 1103 14-15 0.3432730564243087 1103 16-17 0.4751819814840559 1103 18-19 0.2760697882704264 1103 20-21 0.1040076350557797 1103 22-23 0.0037641852096541584 1103 24-25 0.16342055224360763 1103 26-27 0.13277928864927446 1103 28-29 0.20994887537946028 1103 30-31 0.13696992484378967 1103 32-33 0.25029429434123784 1103 34-35 0.13428319263378086 1103 36-37 0.23494389396233117 1103 38-39 0.39984090065015465 1103 40-41 0.22187309411639689 1103 42-43 0.2054619932443913 1103 44-45 0.3607302139504682 1103 46-47 0.46294291044292635 1103 48-49 0.33369808728660644 1103 50-51 -0.29845357463248945 1103 52-53 0.17803014481204116 1103 54-55 0.41080086141654704 1103 56-57 0.5265802006647675 1103 58-59 0.48400145448582776 1103 60-61 0.6661532368256999 1103 62-63 0.5594940735762179 1103 64-65 0.3856795287262216 1103 66-67 0.471548938914502 1103 68-69 0.2143669070507208 1103 70-71 0.22827972079093328 1103 72-73 0.20127836432256174 1103 74-75 0.24567482056001566 1103 76-77 0.20763895904649488 1103 78-79 0.3467414083569942 1103 80-81 0.47468702039581245 1103 82-83 0.4786462450960087 1103 84-85 0.5125693849374926 1103 86-87 0.3131442238058142 1103 88-89 0.4434656004400459 1103 90-91 0.564883282772012 1103 92-93 0.5285393357789765 1103 94-95 0.47099176707860124 1103 96-97 0.3740114441419138 1103 98-99 0.49767020912161897 1103 100-101 0.6316659021708197 1104 1 0.2698533905313383 1104 2 0.25001681473489157 1104 3 0.3001875631696542 1104 4 0.28069017312138556 1104 5 0.24049694859859017 1104 6 0.0895197029544903 1104 7 0.301423798798794 1104 8 0.35755410839544766 1104 9 0.3270537092796957 1104 10-11 0.24345890966480965 1104 12-13 0.2611039757132261 1104 14-15 0.29913382120875553 1104 16-17 0.017423357864970512 1104 18-19 0.2722307443699137 1104 20-21 0.3408644953822062 1104 22-23 -0.00865175340641855 1104 24-25 0.20178975290013312 1104 26-27 0.1914192187836079 1104 28-29 0.210268300143035 1104 30-31 0.27600380085940657 1104 32-33 0.3153719927159031 1104 34-35 0.35684324618839725 1104 36-37 0.4267754551678564 1104 38-39 0.28251176741265027 1104 40-41 0.25636077417512126 1104 42-43 0.2449755161257272 1104 44-45 0.2698249845593139 1104 46-47 0.40385697498711437 1104 48-49 0.5357274539760439 1104 50-51 0.30536032306323335 1104 52-53 0.3943801052237603 1104 54-55 0.612878651540882 1104 56-57 0.7852938703455834 1104 58-59 0.7232030285023185 1104 60-61 0.5694499382552962 1104 62-63 0.5766427658036619 1104 64-65 0.6186964956181882 1104 66-67 0.5732423998818135 1104 68-69 0.41627139225014176 1104 70-71 0.5064052411304765 1104 72-73 0.5217455060486103 1104 74-75 0.4495230394271452 1104 76-77 0.3204162894030631 1104 78-79 0.46620032319631477 1104 80-81 0.7016493588569332 1104 82-83 0.507518505773799 1104 84-85 0.5687218796788791 1104 86-87 0.46789617688435214 1104 88-89 0.43614516643014056 1104 90-91 0.4051003896671723 1104 92-93 0.2482976263807508 1104 94-95 0.1529806279895034 1104 96-97 0.3929076276956174 1104 98-99 0.15809874802517854 1104 100-101 0.18196850616226712 1105 1 -0.00914026264144141 1105 2 0.05382406425962216 1105 3 0.07016482637033405 1105 4 0.12483301272321512 1105 5 0.10092602629747205 1105 6 0.12185930690230862 1105 7 0.17545896674202055 1105 8 -0.00923873268829567 1105 9 0.1438636982288486 1105 10-11 0.16328628781969456 1105 12-13 0.07414249278276941 1105 14-15 0.2395534569965605 1105 16-17 0.21087774242597135 1105 18-19 0.04850826034282818 1105 20-21 -0.3243678899493858 1105 22-23 -0.48816852232517505 1105 24-25 -0.4311358423001579 1105 26-27 -0.25343134074489626 1105 28-29 -0.2291753892307966 1105 30-31 -0.07458324890327361 1105 32-33 -0.16780298748994937 1105 34-35 -0.19748373184963697 1105 36-37 -0.5019434087543146 1105 38-39 -0.28600136446501523 1105 40-41 -0.3450445039801977 1105 42-43 -0.11857616669379922 1105 44-45 0.07464734537580142 1105 46-47 0.24756264987205157 1105 48-49 0.3186555896008372 1105 50-51 0.26585142946044726 1105 52-53 0.12767363857318514 1105 54-55 0.28021323815030286 1105 56-57 0.1995450167121362 1105 58-59 0.19084966392176028 1105 60-61 0.38380947606739824 1105 62-63 0.055366710574830336 1105 64-65 0.2873955018888026 1105 66-67 -0.07868133657455445 1105 68-69 0.12262584074514749 1105 70-71 0.3633772244822282 1105 72-73 0.2930787208288024 1105 74-75 0.0758064830572387 1105 76-77 0.24313657596294647 1105 78-79 0.01650072839402128 1105 80-81 0.28021516220267273 1105 82-83 0.3190867521611054 1105 84-85 -0.17698903694862267 1105 86-87 0.20699563873776583 1105 88-89 0.15759502903551592 1105 90-91 -0.06862103584969859 1105 92-93 0.03163963949780779 1105 94-95 -0.01584963354719804 1105 96-97 0.1764507577526473 1105 98-99 -0.0884229874234812 1105 100-101 -0.13134734058239061 1106 1 -0.0724976213952182 1106 2 0.04913523220506022 1106 3 0.01738640274792047 1106 4 0.21246767132974043 1106 5 0.2457373104949454 1106 6 0.20840894652100417 1106 7 0.32493970765498403 1106 8 0.2367699474636069 1106 9 0.21159988361208804 1106 10-11 0.1334676409933806 1106 12-13 0.3281822509785357 1106 14-15 0.04538349652224838 1106 16-17 0.12424091378146329 1106 18-19 0.1540554199181159 1106 20-21 0.17680431806425645 1106 22-23 0.14784635793523648 1106 24-25 0.028050005952188428 1106 26-27 0.1889754638741934 1106 28-29 0.01418855713826872 1106 30-31 -0.11962122456785096 1106 32-33 0.07346842225971528 1106 34-35 -0.002190001709358569 1106 36-37 -0.16981134644321827 1106 38-39 -0.014300984708377484 1106 40-41 0.006588837103272738 1106 42-43 0.12007957228163235 1106 44-45 -0.007736596343171698 1106 46-47 0.10893209883740695 1106 48-49 -0.022970388853778445 1106 50-51 0.1303499956853571 1106 52-53 0.11478903809267393 1106 54-55 0.23316991489215155 1106 56-57 0.2244617027541409 1106 58-59 0.21492352271429382 1106 60-61 0.055213413136293354 1106 62-63 0.2603965485970008 1106 64-65 -0.044649371396481286 1106 66-67 0.1665232670060135 1106 68-69 0.014521136412820823 1106 70-71 0.21251114682586802 1106 72-73 0.25216769238580383 1106 74-75 0.046569096402972576 1106 76-77 -0.01670609628381925 1106 78-79 0.07329953487313645 1106 80-81 0.031067677121683346 1106 82-83 0.339082102079729 1106 84-85 0.2306293463730853 1106 86-87 0.1710446970962849 1106 88-89 0.23899082846200415 1106 90-91 0.2782265964838686 1106 92-93 0.10338652006822002 1106 94-95 0.24473898925370108 1106 96-97 0.21402922787587642 1106 98-99 0.11396211681584134 1106 100-101 0.27811169995081997 1107 1 0.1286063720625954 1107 2 0.09915567652835122 1107 3 0.19177648043634932 1107 4 0.06373521937390336 1107 5 -0.02256035885220342 1107 6 0.29049290454199195 1107 7 0.23597282745872405 1107 8 0.49017733467908897 1107 9 0.4279008240509654 1107 10-11 0.3989031050795191 1107 12-13 0.6252212314882826 1107 14-15 0.7450393504134922 1107 16-17 0.5279992618801401 1107 18-19 0.7036499408394761 1107 20-21 0.9295813386448728 1107 22-23 0.7573597830408616 1107 24-25 0.8405925982923748 1107 26-27 0.6627896931232016 1107 28-29 0.4141647054277584 1107 30-31 0.27377866639575643 1107 32-33 0.4110893845058712 1107 34-35 0.19797696026420653 1107 36-37 0.15733190011532372 1107 38-39 0.17814824522211126 1107 40-41 -0.028660583640892412 1107 42-43 0.14347128551592192 1107 44-45 0.19292443677662874 1107 46-47 0.37562829619327687 1107 48-49 0.36114437194763127 1107 50-51 0.16089722207215829 1107 52-53 9.141573512252421E-4 1107 54-55 0.32993971181186765 1107 56-57 0.43629896594072903 1107 58-59 0.2146679686731332 1107 60-61 0.13009074719654024 1107 62-63 0.3071799750655799 1107 64-65 0.22857197247703454 1107 66-67 0.21386891236515027 1107 68-69 0.41013582906649404 1107 70-71 0.3137275840617981 1107 72-73 0.42855109159528837 1107 74-75 0.35544874748652333 1107 76-77 0.5313380634544984 1107 78-79 0.4151115833943315 1107 80-81 0.6175130907784911 1107 82-83 0.5388912883956642 1107 84-85 0.5657118051436996 1107 86-87 0.5606283143785546 1107 88-89 0.31361260848108685 1107 90-91 0.5259606839855699 1107 92-93 0.40589435705881627 1107 94-95 0.6683964786908021 1107 96-97 0.41865986710171654 1107 98-99 0.9366246491860188 1107 100-101 1.2660495492080095 1108 1 0.12131037972638126 1108 2 0.026317638149549794 1108 3 -0.06677150589542435 1108 4 0.07419380627789707 1108 5 0.16655381530219415 1108 6 0.18038372125690927 1108 7 0.21224160736865372 1108 8 -0.040644597775248315 1108 9 0.36526477261121926 1108 10-11 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quality scores pass #Quality Count 2 1743.0 3 623.0 4 617.0 5 717.0 6 789.0 7 1005.0 8 1110.0 9 1410.0 10 1770.0 11 2517.0 12 3449.0 13 4653.0 14 5146.0 15 6897.0 16 9120.0 17 12466.0 18 10687.0 19 5910.0 20 5687.0 21 5698.0 22 6183.0 23 6846.0 24 7687.0 25 8962.0 26 10173.0 27 11889.0 28 14418.0 29 17686.0 30 21726.0 31 26804.0 32 34573.0 33 45462.0 34 61288.0 35 85870.0 36 118099.0 37 152066.0 38 120170.0 39 20416.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.05394394392045 18.42134289583182 14.685092312060998 20.839620848186733 2 15.439507963214572 20.780622648992015 39.99676168082845 23.78310770696497 3 19.93846998651831 26.298331391453615 30.03507116901159 23.728127453016484 4 12.082295098514448 16.47744678154053 38.11764457334271 33.32261354660231 5 12.734644242236435 38.57520823355964 33.395897305875614 15.294250218328308 6 29.941910973989078 36.22357935468117 17.729010232496982 16.105499438832773 7 27.54388065026453 34.50188009632853 20.564756526351864 17.38948272705508 8 27.065025537028692 32.03805674477993 19.834619573381403 21.062298144809972 9 27.50020535326629 16.666471092127342 20.973374482216407 34.85994907238996 10-11 25.119582684309933 26.469590490198037 26.23623551949066 22.17459130600138 12-13 27.36018864724362 24.6238528664181 26.980358831189942 21.03559965514833 14-15 23.676254100539268 25.11944443303572 27.638646779503496 23.565654686921516 16-17 21.118255872424644 28.869334187653546 27.72286697189838 22.28954296802343 18-19 20.99369026864915 26.749259033454543 31.28125746320986 20.975793234686446 20-21 22.765121132766957 24.87808684804848 30.9609825105748 21.39580950860976 22-23 21.816089971386717 25.24581199950952 31.42456846859374 21.51352956051003 24-25 22.03861031947259 25.321508228803808 31.391592974864807 21.248288476858797 26-27 21.511756147925663 25.157335273136848 32.05468603341468 21.276222545522806 28-29 20.75412149878656 26.333712490298645 31.686865232283996 21.2253007786308 30-31 20.998171393841407 25.466494033417085 31.493033168932822 22.042301403808686 32-33 20.950497047515466 25.77325998172076 31.488866440698647 21.787376530065128 34-35 21.992047625081543 25.581351692548843 31.07052013084226 21.356080551527352 36-37 21.8436144849725 25.074855919953443 31.585737014023163 21.495792581050893 38-39 21.417223982165904 25.661269506042473 31.34635691657867 21.575149595212952 40-41 21.393716899091654 26.051602805525636 31.23851660999768 21.316163685385032 42-43 21.20099491397162 26.25047663184077 30.191356684871558 22.357171769316057 44-45 21.44665628728745 25.8957494212929 30.2171926164835 22.440401674936144 46-47 22.251025873422325 26.41799318919775 29.75340906757712 21.577571869802803 48-49 22.158501953160517 26.55817274046107 29.126348572424156 22.156976733954252 50-51 21.37161374570366 27.069678273943786 29.528718228796663 22.029989751555892 52-53 22.070052185992378 27.4214042349496 28.408280302603668 22.100263276454356 54-55 21.395640515272625 26.697488224862447 29.029050521191728 22.877820738673194 56-57 22.07791750677119 26.845702546389315 28.35258167517184 22.723798271667658 58-59 22.75127929712134 26.32738625650941 28.475338295846587 22.44599615052266 60-61 22.03093734802786 26.645493470587578 29.612517606036594 21.71105157534797 62-63 21.674589025787412 26.929098845582338 29.40471141318344 21.99160071544681 64-65 21.292731946606846 27.509711739383963 29.019240448705236 22.17831586530395 66-67 21.5632120255021 27.039663413592514 28.43144961645951 22.96567494444588 68-69 21.51004805796993 26.931470461243872 28.928762507274396 22.629718973511796 70-71 22.06232367078055 26.86422774768517 28.55458293171289 22.51886564982139 72-73 21.947486489720923 26.61325520876109 29.121603071168607 22.317655230349377 74-75 21.31183123709405 28.09578562042425 28.318530598836116 22.273852543645578 76-77 21.570218262837592 27.202192601144404 28.34237335463986 22.885215781378143 78-79 21.705596946607887 27.24168931829172 28.51792647259036 22.534787262510033 80-81 22.038830963850472 27.377296383873826 28.072299053575932 22.511573598699766 82-83 21.830833057610764 27.163190215720647 28.547610834606353 22.45836589206224 84-85 21.531677880776677 27.42684428549722 28.175077395839015 22.866400437887087 86-87 21.69220786992114 27.520553618927046 28.06638170654583 22.720856804605983 88-89 21.423878278458673 27.43064110777115 27.571670268659375 23.573810345110793 90-91 21.502400687117486 27.03014117823753 28.014997841024293 23.452460293620696 92-93 22.042248356503144 27.433270067382637 28.02853714845595 22.49594442765827 94-95 21.5847912974458 27.125260251213916 28.033741580884232 23.256206870456055 96-97 21.009025498426332 27.18795739800483 28.59988912452372 23.20312797904512 98-99 21.402133694464624 27.669494459224282 27.385385213879925 23.54298663243117 100-101 21.23596672803579 27.192077924898044 27.804145438368632 23.767809908697533 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 356.0 1 283.0 2 335.0 3 484.0 4 406.0 5 362.5 6 651.5 7 1343.5 8 2278.5 9 2776.0 10 2331.5 11 1760.5 12 1760.0 13 1872.0 14 1956.5 15 2032.0 16 2054.0 17 2134.0 18 2188.5 19 2255.0 20 2468.5 21 2775.5 22 3091.5 23 3506.5 24 4025.0 25 4640.5 26 5492.0 27 6595.0 28 7608.5 29 8727.0 30 10273.0 31 12069.5 32 14279.5 33 15850.0 34 16862.5 35 17778.0 36 19318.5 37 21105.0 38 21575.0 39 21536.0 40 21843.0 41 22954.5 42 23965.5 43 24773.5 44 25768.0 45 27239.0 46 28227.0 47 32628.5 48 43440.0 49 42867.5 50 32338.5 51 27308.5 52 25294.5 53 25062.5 54 24953.0 55 23614.0 56 21714.0 57 19745.5 58 18890.0 59 17254.0 60 14685.0 61 12615.5 62 10779.0 63 8653.5 64 6372.5 65 4883.5 66 3652.5 67 2837.0 68 2086.0 69 1383.5 70 1022.5 71 833.0 72 720.0 73 723.5 74 627.0 75 453.0 76 342.0 77 235.5 78 160.5 79 106.0 80 73.0 81 54.5 82 42.5 83 28.5 84 17.0 85 13.0 86 10.0 87 7.5 88 7.0 89 5.0 90 3.5 91 3.0 92 2.5 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014313536851491188 2 0.005396907337447498 3 0.008564657296384072 4 0.03906891616021776 5 0.04833751789192106 6 0.06382429546894432 7 0.030034962573620854 8 0.028627073702982377 9 0.01759861088298097 10-11 0.04915878639979351 12-13 0.04452448553394186 14-15 0.01941713400755567 16-17 0.023523476546917898 18-19 0.028568411666705776 20-21 0.02305418025670507 22-23 0.013316282234788934 24-25 0.003402398104042988 26-27 0.0023464814510641293 28-29 0.001231902761808668 30-31 0.006042189736490133 32-33 8.212685078724453E-4 34-35 0.0018771851608513034 36-37 0.004223666611915433 38-39 0.0046929629021282586 40-41 0.0025811295961705423 42-43 5.866203627660323E-4 44-45 0.0010559166529788583 46-47 0.002053171269681113 48-49 4.1063425393622263E-4 50-51 0.0018185231245747004 52-53 4.6929629021282584E-4 54-55 0.001701199052021494 56-57 0.0018185231245747004 58-59 0.0019358471971279065 60-61 5.279583264894292E-4 62-63 6.452823990426356E-4 64-65 0.002463805523617336 66-67 0.0021118333059577167 68-69 0.004223666611915433 70-71 0.00745007860712861 72-73 0.003050425886383368 74-75 0.0023464814510641293 76-77 0.002229157378510923 78-79 0.03566651805617477 80-81 0.021704953422343194 82-83 0.00398901846680902 84-85 0.007684726752235024 86-87 0.006687472135532768 88-89 0.00398901846680902 90-91 0.009855222094469344 92-93 0.01378557852500176 94-95 0.004516976793298449 96-97 0.00381303235797921 98-99 0.023816786728300912 100-101 0.04593237440458033 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 852340.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.40694831793703 #Duplication Level Percentage of deduplicated Percentage of total 1 80.05542773186008 44.356269469095146 2 11.465982402373722 12.705901887653928 3 3.565262816296478 5.926209978072047 4 1.531598056082485 3.3944469734886025 5 0.8195088536722595 2.2703242350755355 6 0.5058795764250594 1.681754612764989 7 0.3422071365884326 1.327245719168915 8 0.2570631571678125 1.1394468050914168 9 0.18363574203626626 0.9157226461496479 >10 1.1260939429757744 11.793953696064127 >50 0.0965344404913327 3.6410698622388686 >100 0.04509045283292858 4.770743951859498 >500 0.0025403071988139734 1.0587969126404875 >1k 0.0025403071988139734 2.8704926158248196 >5k 6.350767997034934E-4 2.1476206348119335 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7400 0.8681981368937278 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5740 0.6734401764554051 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5170 0.6065654551000774 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4186 0.4911185677077223 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3569 0.41872961494239386 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3105 0.3642912452777061 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2717 0.318769505127062 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1789 0.20989276579768634 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1577 0.1850200624164066 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1513 0.17751132177300138 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1294 0.15181734988384915 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1292 0.15158270173874278 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1280 0.15017481286810427 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1134 0.13304549827533613 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1017 0.11931858178661098 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 932 0.10934603561958843 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 898 0.10535701715277941 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 884 0.1037144801370345 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1732407255320646E-4 2 0.0 0.0 0.0 0.0 1.1732407255320646E-4 3 0.0 0.0 0.0 0.0 1.1732407255320646E-4 4 0.0 0.0 0.0 0.0 1.1732407255320646E-4 5 0.0 0.0 0.0 0.0 2.3464814510641292E-4 6 0.0 0.0 0.0 0.0 2.3464814510641292E-4 7 0.0 0.0 0.0 0.0 2.3464814510641292E-4 8 0.0 0.0 0.0 0.0 2.3464814510641292E-4 9 0.0 0.0 0.0 0.0 2.3464814510641292E-4 10-11 0.0 0.0 0.0 0.0 2.3464814510641292E-4 12-13 0.0 0.0 0.0 0.0 4.692962902128259E-4 14-15 0.0 0.0 0.0 0.0 7.039444353192387E-4 16-17 0.0 0.0 0.0 0.0 8.212685078724453E-4 18-19 0.0 0.0 0.0 0.0 8.799305441490484E-4 20-21 0.0 0.0 0.0 0.0 0.0012905647980852712 22-23 0.0 0.0 0.0 2.3464814510641292E-4 0.0012905647980852712 24-25 0.0 0.0 0.0 2.3464814510641292E-4 0.0012905647980852712 26-27 0.0 0.0 0.0 4.1063425393622263E-4 0.0012905647980852712 28-29 0.0 0.0 0.0 0.0012319027618086678 0.0012905647980852712 30-31 0.0 0.0 0.0 0.002522467559893939 0.0012905647980852712 32-33 0.0 0.0 0.0 0.004868949010958069 0.0012905647980852712 34-35 0.0 0.0 0.0 0.007156768425745594 0.0014078888706384777 36-37 0.0 0.0 0.0 0.00979656005819274 0.0014078888706384777 38-39 0.0 0.0 0.0 0.01396156463383157 0.0014078888706384777 40-41 0.0 0.0 0.0 0.01976910622521529 0.0014078888706384777 42-43 0.0 0.0 0.0 0.030386934791280476 0.0014078888706384777 44-45 0.0 0.0 0.0 0.047281601238942206 0.0014665509069150809 46-47 0.0 0.0 0.0 0.06564281859351902 0.001525212943191684 48-49 0.0 0.0 0.0 0.10312785977426848 0.001525212943191684 50-51 0.0 0.0 0.0 0.1438393129502311 0.001525212943191684 52-53 0.0 0.0 0.0 0.18284956707417227 0.0016425370157448905 54-55 0.0 0.0 0.0 0.2392824459722646 0.001759861088298097 56-57 0.0 0.0 0.0 0.3049839266020602 0.001759861088298097 58-59 0.0 0.0 0.0 0.39068916160217754 0.001759861088298097 60-61 0.0 0.0 0.0 0.49141187788910534 0.001759861088298097 62-63 0.0 0.0 0.0 0.6204096956613558 0.00199450923340451 64-65 0.0 0.0 0.0 0.733920735856583 0.00199450923340451 66-67 0.0 0.0 0.0 0.8767627941901119 0.00199450923340451 68-69 0.0 0.0 0.0 1.0684116667057748 0.00199450923340451 70-71 0.0 0.0 0.0 1.220932961024943 0.00199450923340451 72-73 0.0 0.0 0.0 1.4087688011826267 0.00199450923340451 74-75 0.0 0.0 0.0 1.6255836872609521 0.0021118333059577162 76-77 0.0 0.0 0.0 1.8232160874768284 0.002229157378510923 78-79 0.0 0.0 0.0 2.041086890208133 0.002229157378510923 80-81 0.0 0.0 0.0 2.3240725532064666 0.002229157378510923 82-83 0.0 0.0 0.0 2.6757514606847033 0.002229157378510923 84-85 0.0 0.0 0.0 3.046554191989112 0.002229157378510923 86-87 0.0 0.0 0.0 3.4492690710279934 0.002229157378510923 88-89 0.0 0.0 0.0 3.8926367412065606 0.002229157378510923 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCGAC 25 0.0050101588 57.03065 7 AGGTAAG 205 3.092282E-11 27.816563 2 GTACATG 6760 0.0 26.713972 1 TACATGG 6805 0.0 25.767622 2 GTATTAG 225 1.0186341E-10 25.345469 1 ACATGGG 6815 0.0 24.614155 3 GAGTACT 5225 0.0 24.23959 12-13 GTCTAGG 180 1.9408071E-7 23.761374 1 CTACGCA 125 2.2488297E-4 22.809582 4 GTACTTT 5660 0.0 22.374025 14-15 CATGGGT 770 0.0 22.217125 4 CATGGGA 4485 0.0 21.932287 4 GGTAAGG 285 8.367351E-11 21.675772 3 TTACGTG 110 0.0028678016 21.602518 7 ACTAGTA 110 0.0028697567 21.599983 2 AGTACTT 5385 0.0 21.577816 12-13 GGGATAC 180 5.142161E-6 21.122463 7 ATGGGAT 1620 0.0 20.827871 5 GATCGCG 80 4.9929993E-5 20.780224 84-85 CGCGTGC 115 0.0037109214 20.663279 9 >>END_MODULE