FastQCFastQC Report
Thu 26 May 2016
SRR936414_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936414_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852340
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT84300.9890419316235305No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75110.8812211089471337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63000.7391416570852007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43010.504610836051341No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26900.31560175516812544No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25540.2996456813008893No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC18320.21493770091747427No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT17190.2016800807189619No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT17110.20074148813853626No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16050.1883051364478964No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA15650.18361217354576811No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC15210.17844991435342702No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14910.17493019217683084No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG14790.17352230330619237No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA12760.14970551657789144No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12090.14184480371682662No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12010.14090621113640095No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC10290.12072647065724945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC1456.6393113E-1032.756688
ATACGTC953.4912093E-529.998225
TGCACCG1457.887629E-726.2053435
GGACCGT1000.001653866523.7485926
GAGTACT65000.023.52523212-13
GTACATG67850.023.3490331
ACACCGT1252.256144E-422.7986496
AGGCGAC2103.4457116E-822.6177065
TACATGG69200.022.4471132
GTACTTT67750.022.395114-15
AGGTAAG3007.2759576E-1222.1679522
GCGACAG1954.2212014E-721.9217787
TGCGATA550.002881372321.58646256-57
AGTACTT65550.021.51667612-13
GCACCGT2005.385791E-721.3737346
ACATGGG68950.021.0829543
ACTTTTT78100.019.76167516-17
AGAGTAC113450.019.3806310-11
TACTTTT72800.019.34125914-15
AGTGCGT1250.006031957518.9988758
Produced by FastQC (version 0.11.5)