##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936414_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 852340 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.430160499331254 31.0 31.0 34.0 30.0 34.0 2 31.654375014665508 31.0 31.0 34.0 30.0 34.0 3 31.886100617124622 31.0 31.0 34.0 30.0 34.0 4 35.72119576694746 37.0 35.0 37.0 33.0 37.0 5 35.548759884553114 37.0 35.0 37.0 33.0 37.0 6 35.68204589717718 37.0 35.0 37.0 33.0 37.0 7 35.61965412863411 37.0 35.0 37.0 33.0 37.0 8 35.600326160921696 37.0 35.0 37.0 33.0 37.0 9 37.20096909683929 39.0 37.0 39.0 33.0 39.0 10-11 37.07989886664946 39.0 37.0 39.0 33.0 39.0 12-13 36.95337776004881 39.0 37.0 39.0 33.0 39.0 14-15 37.95783842128728 40.0 37.0 41.0 33.0 41.0 16-17 37.91064891944529 40.0 37.0 41.0 33.0 41.0 18-19 38.03772320904803 40.0 37.0 41.0 33.0 41.0 20-21 38.13188105685525 40.0 37.0 41.0 33.0 41.0 22-23 38.0430661473121 40.0 37.0 41.0 33.0 41.0 24-25 37.904403172442926 40.0 37.0 41.0 33.0 41.0 26-27 37.65260635427177 39.5 37.0 41.0 32.0 41.0 28-29 37.54597578431142 39.0 36.5 41.0 32.0 41.0 30-31 37.46938076354506 39.0 36.5 41.0 32.0 41.0 32-33 37.21210373794495 39.0 35.5 41.0 32.0 41.0 34-35 36.98738590233944 39.0 35.0 41.0 31.0 41.0 36-37 36.694448224886784 39.0 35.0 41.0 30.5 41.0 38-39 36.49784593002792 39.0 35.0 40.0 30.0 41.0 40-41 36.21694746228031 39.0 35.0 40.0 30.0 41.0 42-43 35.88519487528451 38.0 35.0 40.0 29.5 41.0 44-45 35.47024016237651 38.0 35.0 40.0 27.5 41.0 46-47 34.8757766853603 38.0 34.0 40.0 25.0 41.0 48-49 34.20522854729333 38.0 33.0 40.0 23.0 41.0 50-51 33.95718785930497 38.0 33.0 40.0 22.0 41.0 52-53 33.70003695708286 38.0 33.0 40.0 20.5 41.0 54-55 33.40864091794354 37.5 33.0 40.0 19.5 41.0 56-57 33.084805945984 37.0 32.5 40.0 18.0 41.0 58-59 32.682875378370134 36.5 32.0 39.5 15.0 41.0 60-61 32.2496140038013 36.0 31.0 39.0 13.0 40.5 62-63 31.9743729028322 35.5 31.0 39.0 10.0 40.0 64-65 31.5337928526175 35.0 31.0 38.5 8.0 40.0 66-67 31.103699814627966 35.0 30.0 38.0 7.0 40.0 68-69 30.620534645798624 35.0 29.5 37.5 3.5 40.0 70-71 30.103245770467183 34.0 29.0 37.0 2.0 39.0 72-73 29.669865898585073 34.0 29.0 36.0 2.0 39.0 74-75 29.302434474505482 34.0 28.5 36.0 2.0 39.0 76-77 27.972149025036956 32.0 26.5 34.5 2.0 36.5 78-79 28.768392894854166 33.5 28.5 35.0 2.0 37.0 80-81 28.58428268062041 34.0 28.0 35.0 2.0 37.0 82-83 28.232942839711853 33.5 27.5 35.0 2.0 36.0 84-85 27.78107386723608 33.0 26.5 35.0 2.0 36.0 86-87 27.408727151136873 33.0 26.0 35.0 2.0 35.5 88-89 26.96035384940282 33.0 25.5 35.0 2.0 35.0 90-91 26.463584954360936 32.5 25.0 34.5 2.0 35.0 92-93 25.967853204120424 32.0 24.0 34.0 2.0 35.0 94-95 25.394435319238802 32.0 21.5 34.0 2.0 35.0 96-97 24.655442663725744 31.5 18.0 34.0 2.0 35.0 98-99 23.77285590257409 31.0 2.0 34.0 2.0 35.0 100-101 21.064965858694887 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2776011048538223 1101 2 0.3283066528985401 1101 3 0.16797057349269195 1101 4 0.11463825905389058 1101 5 0.1544948405887112 1101 6 0.08721957507255951 1101 7 0.19096256033147796 1101 8 0.11190465643258563 1101 9 0.14173238489873086 1101 10-11 0.13499162951404742 1101 12-13 0.1343806842081392 1101 14-15 0.20477462510836375 1101 16-17 0.18560717272910665 1101 18-19 0.1907146395897712 1101 20-21 0.10656728969977536 1101 22-23 0.0848558095172649 1101 24-25 0.09850791376288726 1101 26-27 0.08473414749477826 1101 28-29 0.19675698086346927 1101 30-31 0.1583383499615465 1101 32-33 0.15806085623486155 1101 34-35 0.20958186646038257 1101 36-37 0.29827632684594363 1101 38-39 0.5075279570994908 1101 40-41 0.5459501406081344 1101 42-43 0.6894889200691878 1101 44-45 0.6811364402016977 1101 46-47 0.5238562021426674 1101 48-49 0.5542291315481123 1101 50-51 0.40550310904377085 1101 52-53 0.43234582054628135 1101 54-55 0.48233536382127085 1101 56-57 0.42649253653092245 1101 58-59 0.46366508017172947 1101 60-61 0.3698373068115757 1101 62-63 0.28758349274916384 1101 64-65 0.3073577684498012 1101 66-67 0.21746978703326292 1101 68-69 0.21202803013083482 1101 70-71 0.34131680422547106 1101 72-73 0.3918160596020215 1101 74-75 0.13942452863758703 1101 76-77 0.5281541723722221 1101 78-79 0.4069697937637713 1101 80-81 0.19793463647957665 1101 82-83 0.2775380630993709 1101 84-85 0.6219673595908475 1101 86-87 0.5117217670144534 1101 88-89 0.16194375238747583 1101 90-91 0.21331080374406852 1101 92-93 0.3471620280759602 1101 94-95 0.08331562586836583 1101 96-97 0.2345594971697338 1101 98-99 0.21555790810718278 1101 100-101 -0.09193668885345829 1102 1 0.49152224621633067 1102 2 0.47753322250727237 1102 3 0.1316791443808114 1102 4 0.05814925704256524 1102 5 0.08824367057751914 1102 6 0.16488222701094202 1102 7 0.17904230698940182 1102 8 0.03880696999139133 1102 9 0.12300708092877244 1102 10-11 0.19554451308786014 1102 12-13 0.19041356239605278 1102 14-15 0.2841219999736566 1102 16-17 0.30391842435803795 1102 18-19 0.11630821788815382 1102 20-21 0.22367183989599937 1102 22-23 0.07976297038175062 1102 24-25 0.1387395258927029 1102 26-27 -0.07091892258195998 1102 28-29 0.09507957269042322 1102 30-31 0.03640652812659084 1102 32-33 0.1728126647728061 1102 34-35 0.34171217903332973 1102 36-37 0.18281829512973502 1102 38-39 0.1402639499282401 1102 40-41 0.3055523857250648 1102 42-43 0.150605412416418 1102 44-45 -0.011739641811139734 1102 46-47 0.00769013020742193 1102 48-49 0.12645953045378633 1102 50-51 -0.023581750021421044 1102 52-53 -0.24485479053851122 1102 54-55 -0.1326723679201578 1102 56-57 -0.0778630033701333 1102 58-59 -0.06570482134000599 1102 60-61 0.017724624193157013 1102 62-63 -0.027174791908439744 1102 64-65 -0.19835868839524906 1102 66-67 -0.273376986577901 1102 68-69 -0.24794256176320673 1102 70-71 -0.4407025989744824 1102 72-73 -0.09733131498373027 1102 74-75 -0.5365199947782457 1102 76-77 -0.501391376413693 1102 78-79 -0.2401191904498745 1102 80-81 0.006195369716092358 1102 82-83 -0.07846937651780195 1102 84-85 0.22595543793978123 1102 86-87 -0.08332204463389559 1102 88-89 -0.09357481008168378 1102 90-91 -0.049259507580242 1102 92-93 0.10585016191452112 1102 94-95 0.044568629227097034 1102 96-97 0.15893565815395405 1102 98-99 -0.18431242972728157 1102 100-101 -0.35726004989433235 1103 1 0.4498400128772282 1103 2 0.489761792894452 1103 3 0.23896920692086354 1103 4 0.19901017755685757 1103 5 0.025785624658112738 1103 6 0.12106318312356024 1103 7 0.013721208301255672 1103 8 0.11947118851406202 1103 9 -0.08031340420467359 1103 10-11 0.1282327027673844 1103 12-13 0.22393658132816796 1103 14-15 0.25278970543094914 1103 16-17 0.13215500133029678 1103 18-19 0.09376691684845895 1103 20-21 0.08067259013368044 1103 22-23 0.08719244955885586 1103 24-25 -0.0242852987734139 1103 26-27 0.20861750950634672 1103 28-29 0.4425117551530775 1103 30-31 0.3474491458789686 1103 32-33 0.28318340975578593 1103 34-35 0.4816026504073534 1103 36-37 0.39351206754954404 1103 38-39 0.2697946109574971 1103 40-41 0.48186583320386944 1103 42-43 0.3884960599596141 1103 44-45 0.557100739807737 1103 46-47 0.752856108903103 1103 48-49 0.946406284840549 1103 50-51 0.72753373494497 1103 52-53 0.7035451074048709 1103 54-55 0.40449021496288395 1103 56-57 0.532910197046597 1103 58-59 0.6805553805255684 1103 60-61 0.6110730230280055 1103 62-63 0.3897060114229802 1103 64-65 0.43434605511155056 1103 66-67 0.4596348380183528 1103 68-69 0.4386114781299959 1103 70-71 0.47955199585161523 1103 72-73 0.40523149084443233 1103 74-75 0.6027227511523989 1103 76-77 0.30703983711853056 1103 78-79 0.5149593415141993 1103 80-81 0.6137291835660257 1103 82-83 0.6256218487387812 1103 84-85 0.5794851998987802 1103 86-87 0.606284581756853 1103 88-89 0.43638382053712377 1103 90-91 0.3348021941532018 1103 92-93 0.5242086601027118 1103 94-95 0.6346882723934044 1103 96-97 0.46217823399022606 1103 98-99 0.3344101007909863 1103 100-101 0.5799994260327601 1104 1 0.35239507117224633 1104 2 0.41653196677706106 1104 3 0.14744721588514764 1104 4 0.0472511325689382 1104 5 0.008937667447440845 1104 6 5.19413435611682E-4 1104 7 0.041917239958287666 1104 8 -0.06357958982894019 1104 9 -0.013509452852389359 1104 10-11 0.03994743606469342 1104 12-13 0.07137302769368858 1104 14-15 0.24490942855084796 1104 16-17 0.13903112908661086 1104 18-19 0.02555019054450014 1104 20-21 -0.07532223110739267 1104 22-23 0.00918824266675955 1104 24-25 0.006049859358846277 1104 26-27 0.13923097495919734 1104 28-29 -0.0778405295835114 1104 30-31 0.1107341577214811 1104 32-33 0.04967965991294676 1104 34-35 0.028702511423595922 1104 36-37 -0.10973994839451251 1104 38-39 0.013453689586960138 1104 40-41 0.0702887244057564 1104 42-43 0.09614084602997508 1104 44-45 0.28231303834076726 1104 46-47 0.21823675108279872 1104 48-49 0.3076595512985705 1104 50-51 0.39296178111883506 1104 52-53 0.32373632050535406 1104 54-55 0.37843840698474196 1104 56-57 0.4202441556369152 1104 58-59 0.4225909997848376 1104 60-61 0.20102677440000605 1104 62-63 -0.004614514336587661 1104 64-65 0.12762097449077814 1104 66-67 0.2959067092697829 1104 68-69 0.13489629627876099 1104 70-71 0.07961350210928231 1104 72-73 0.3197930123937134 1104 74-75 0.4338787289829611 1104 76-77 0.5618456506492286 1104 78-79 0.4162528418998903 1104 80-81 0.2942576956243137 1104 82-83 0.369824629093479 1104 84-85 0.4562373636617387 1104 86-87 0.4987845562708344 1104 88-89 0.5135682271716462 1104 90-91 0.35389377051441073 1104 92-93 0.6645610128020216 1104 94-95 0.4933988837520147 1104 96-97 0.37178697263620464 1104 98-99 0.2568918273158154 1104 100-101 0.2432050914047359 1105 1 0.1893188711392213 1105 2 0.27508536438313413 1105 3 0.052744424382023425 1105 4 0.043815208426011054 1105 5 -0.22061297379804046 1105 6 -0.06793867591112246 1105 7 0.030284212746110484 1105 8 -0.18301976494887384 1105 9 -0.09365561194223915 1105 10-11 -0.10773273295821184 1105 12-13 -0.08785337070414556 1105 14-15 -0.08786501139140057 1105 16-17 -0.0738268061410281 1105 18-19 0.02197030901296415 1105 20-21 -0.14282660275456038 1105 22-23 -0.2877090522045478 1105 24-25 -0.28365697565329384 1105 26-27 -0.21382134508348116 1105 28-29 -0.30556418220586323 1105 30-31 -0.123621061368965 1105 32-33 -0.08557677477062242 1105 34-35 0.0526724751378751 1105 36-37 0.12049025640603617 1105 38-39 0.06123272346351172 1105 40-41 -0.2534206340947094 1105 42-43 -0.01117727016056591 1105 44-45 0.17866477704963302 1105 46-47 0.2693617357344067 1105 48-49 0.4800601677749228 1105 50-51 0.4139880279380108 1105 52-53 0.6376258797840464 1105 54-55 0.6346126970419448 1105 56-57 0.5166130422111976 1105 58-59 0.7369441865356805 1105 60-61 0.7252296103605644 1105 62-63 0.5351314855533289 1105 64-65 0.36172320964093885 1105 66-67 0.5026125831546437 1105 68-69 0.5148644139512228 1105 70-71 0.5947777193833232 1105 72-73 0.5582002085695272 1105 74-75 0.41466571015418197 1105 76-77 0.2837464386042292 1105 78-79 0.4623192062560406 1105 80-81 0.4075733306669278 1105 82-83 0.517786361832357 1105 84-85 0.7191384861059689 1105 86-87 0.6169090031992503 1105 88-89 0.8011792119672023 1105 90-91 0.2758868605597584 1105 92-93 0.24870622346672633 1105 94-95 0.689535533186131 1105 96-97 0.7884388201599286 1105 98-99 0.8286799897844297 1105 100-101 1.006110447889803 1106 1 0.2645145620638125 1106 2 0.40100569298888544 1106 3 0.1890305593893835 1106 4 0.017658500683630507 1106 5 -0.2406664497338724 1106 6 0.0066563795023526495 1106 7 0.062331023565299404 1106 8 -0.054658143620500255 1106 9 -0.0988675781516477 1106 10-11 -0.01347170839028422 1106 12-13 0.15892063534095513 1106 14-15 0.03979909773050849 1106 16-17 0.12481885765808443 1106 18-19 0.13843547215332563 1106 20-21 0.04556950667736004 1106 22-23 -0.08506238088779838 1106 24-25 -0.14181980600282174 1106 26-27 -0.08000556580734042 1106 28-29 -0.2252243387586006 1106 30-31 -0.26307506431401606 1106 32-33 -0.2241491997880658 1106 34-35 -0.4425224407731534 1106 36-37 -0.16177937081243243 1106 38-39 -0.19362079897312157 1106 40-41 -0.18022060309417043 1106 42-43 -0.2430032021143802 1106 44-45 0.019932311731487573 1106 46-47 0.18855688414949867 1106 48-49 -0.24030525111991352 1106 50-51 -0.05728803189303022 1106 52-53 0.10014428635616213 1106 54-55 -0.020796897625963595 1106 56-57 -0.2292309475586265 1106 58-59 -0.19228738751686336 1106 60-61 0.1288857368427756 1106 62-63 -0.30391757280519727 1106 64-65 0.037709414399522245 1106 66-67 -0.05532936832950597 1106 68-69 -0.20750635253719096 1106 70-71 -0.2663689131827489 1106 72-73 0.047543772081887425 1106 74-75 0.1995606958164302 1106 76-77 0.1693544367441966 1106 78-79 0.2136367293126895 1106 80-81 0.44669214024609616 1106 82-83 0.4365261897793431 1106 84-85 0.3129829408839271 1106 86-87 0.00989900552428935 1106 88-89 0.3249527530041689 1106 90-91 -0.13880352152192899 1106 92-93 0.18766421773162634 1106 94-95 0.344199177299906 1106 96-97 0.43938239917256183 1106 98-99 0.46409423453371446 1106 100-101 0.33491963955065884 1107 1 0.5426595804637024 1107 2 0.5025117581381302 1107 3 0.3556517942265103 1107 4 0.0885321547656801 1107 5 0.034411920172082944 1107 6 0.1219623628742994 1107 7 0.006926907441538788 1107 8 0.029470246729779603 1107 9 0.19536030790532521 1107 10-11 0.295442016565417 1107 12-13 0.04809536615736931 1107 14-15 0.19248411885630645 1107 16-17 0.4371910806375041 1107 18-19 0.25973845702451825 1107 20-21 0.24508906507997352 1107 22-23 0.14214219864062727 1107 24-25 0.07899592249656706 1107 26-27 0.1784277175509814 1107 28-29 0.33571678159031393 1107 30-31 0.20481031118005433 1107 32-33 0.280041886486984 1107 34-35 0.15172588960710698 1107 36-37 0.47708869515458474 1107 38-39 0.3497722772160543 1107 40-41 0.6787817139291406 1107 42-43 0.597326632968084 1107 44-45 0.23069624794519683 1107 46-47 0.4000704455506181 1107 48-49 0.5633781344600237 1107 50-51 0.4702606937441516 1107 52-53 0.48621829480648415 1107 54-55 0.4267634131277518 1107 56-57 0.40878527160452194 1107 58-59 0.30139190919840786 1107 60-61 0.28949293324457415 1107 62-63 0.219116364771466 1107 64-65 0.3733711289967694 1107 66-67 0.3759606684702739 1107 68-69 0.22352813022142826 1107 70-71 0.17265589259617897 1107 72-73 0.23852782550971696 1107 74-75 0.4600895223122734 1107 76-77 0.5059020720174701 1107 78-79 0.6248061446050457 1107 80-81 0.6741693107517506 1107 82-83 0.8254112926824391 1107 84-85 0.7394685617098808 1107 86-87 1.1329056840032337 1107 88-89 1.1962557378753722 1107 90-91 0.9007562440498305 1107 92-93 1.0925810774835654 1107 94-95 1.4241535183112148 1107 96-97 1.6840839120524826 1107 98-99 1.763433201413907 1107 100-101 1.592194411255715 1108 1 0.202318216977023 1108 2 0.21195506451421053 1108 3 -0.015276285079984575 1108 4 -0.04525906694951942 1108 5 -0.1741589463232316 1108 6 -0.17583044976068152 1108 7 0.02935160604349818 1108 8 0.10362122159581588 1108 9 0.10303564052303216 1108 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-0.7249084825871606 2316 80-81 -0.724518278189862 2316 82-83 -0.5543219204583849 2316 84-85 -0.5727222000101584 2316 86-87 -0.6599369532770396 2316 88-89 -0.8042975359356603 2316 90-91 -0.6236700983671533 2316 92-93 -0.9370197044429922 2316 94-95 -1.2016472727310656 2316 96-97 -0.7750548545900422 2316 98-99 -1.3032964312730506 2316 100-101 -2.3106509607417927 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 11.0 5 43.0 6 79.0 7 250.0 8 496.0 9 934.0 10 1555.0 11 2508.0 12 3491.0 13 4855.0 14 6241.0 15 8658.0 16 11230.0 17 14705.0 18 12304.0 19 7410.0 20 6339.0 21 6580.0 22 6908.0 23 7379.0 24 8653.0 25 10202.0 26 12347.0 27 14862.0 28 18129.0 29 22207.0 30 27396.0 31 33233.0 32 41389.0 33 52367.0 34 68320.0 35 91013.0 36 122153.0 37 144840.0 38 79101.0 39 4143.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.883101790087224 19.599409431310814 15.454518502706774 20.062970275895193 2 16.00657548905493 22.439267074681783 38.384358690346566 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31.624527770607973 20.85470586855011 22-23 21.716412657069444 25.380955733108067 31.821536259430033 21.081095350392452 24-25 22.31949691437689 25.478975526198468 31.832484689208535 20.369042870216113 26-27 21.549029729919987 25.35232418987728 32.582302836896076 20.51634324330666 28-29 20.617007297557315 26.881643475608325 31.968756599479082 20.53259262735528 30-31 21.211547035220686 25.455334725578993 31.87084966093343 21.462268578266887 32-33 21.053922186899975 25.999355869303653 31.81958708758476 21.127134856211608 34-35 22.076927860185773 25.5122129782913 31.685870864722744 20.724988296800188 36-37 22.152438956426654 25.069648014276304 32.01356530370288 20.764347725594156 38-39 21.4455624783317 25.88721982261763 31.926538662117622 20.740679036933052 40-41 21.35085033115691 26.16954999032048 31.45667974868446 21.022919929838146 42-43 21.28091100563805 26.413120640191494 30.500149604867143 21.80581874930331 44-45 21.838709601725164 25.85536520063919 30.399283842097972 21.90664135553767 46-47 22.64983738578287 26.842609148633617 29.690469821335547 20.817083644247962 48-49 22.68719972918792 27.007520667314754 28.947030307368188 21.358249296129138 50-51 21.638422785326945 27.410370064457997 29.543913330157757 21.4072938200573 52-53 22.42436152769915 27.812959220487432 28.240435747995928 21.52224350381749 54-55 21.562150923205454 27.242363725112877 28.76674395246454 22.428741399217127 56-57 22.578828465481145 27.08476204946677 28.336948021816017 21.999461463236067 58-59 23.317367341970417 26.528907439291594 28.262209877013962 21.891515341724034 60-61 22.149451127078514 27.02999676167323 29.62922331783004 21.19132879341822 62-63 21.83921869078613 27.146541235438153 29.438891580909832 21.575348492865885 64-65 21.515842785495185 28.024515774725234 28.756691614272 21.702949825507577 66-67 22.111341333496025 27.153133344909637 28.169599068889944 22.565926252704397 68-69 21.859260223924988 27.336423653030074 28.558061991766188 22.24625413127875 70-71 22.668769923351196 27.29591507504731 28.280863375527527 21.75445162607397 72-73 22.37582559531736 26.8642366099424 28.916059856430447 21.843877938309795 74-75 21.828437008705446 28.532451838468216 27.841941009456324 21.797170143370018 76-77 22.11500612733236 27.373424683088736 27.97348674836052 22.53808244121838 78-79 22.144799077258483 27.640238758194553 28.03390582263509 22.181056341911873 80-81 22.399397325632496 27.830331206880064 27.836491721734824 21.93377974575262 82-83 22.357246114925914 27.415293416377622 28.197881807386686 22.029578661309777 84-85 21.783005358015522 27.939355219474475 27.892773983030118 22.38486543947989 86-87 21.93918678696015 27.865858452165977 27.370698000232323 22.82425676064155 88-89 21.636294820600238 27.970108352820017 27.065085042146986 23.328511784432752 90-91 22.15632739927862 27.39341086977059 27.329217694080203 23.12104403687059 92-93 22.498514346456165 27.841411397265226 27.45258961292052 22.20748464335809 94-95 21.946344436824266 27.687486176614325 27.394503733854137 22.97166565270727 96-97 21.43401920567722 27.67920839943303 28.075806572734695 22.81096582215505 98-99 21.712020435450157 28.09440248661036 26.761393004002958 23.43218407393653 100-101 21.199327892012146 27.874411553498764 27.035039261056692 23.891221293432395 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 773.0 1 560.0 2 452.0 3 566.5 4 479.5 5 440.0 6 716.0 7 1448.5 8 2254.5 9 2587.5 10 2303.0 11 1880.0 12 1849.0 13 2025.5 14 2183.0 15 2245.5 16 2320.5 17 2419.5 18 2482.5 19 2592.0 20 2820.0 21 3103.0 22 3402.5 23 3813.5 24 4203.5 25 4805.0 26 5699.5 27 6774.0 28 7869.5 29 9184.0 30 10735.5 31 12368.0 32 14401.5 33 15691.5 34 16775.5 35 17837.5 36 19321.0 37 20977.0 38 21602.0 39 21553.5 40 21489.0 41 22860.0 42 23791.0 43 24366.0 44 25678.0 45 27011.5 46 27643.5 47 32263.5 48 43767.0 49 42712.0 50 31562.5 51 26815.0 52 24938.5 53 24481.5 54 24071.0 55 22886.5 56 21402.5 57 19851.5 58 18958.5 59 17603.5 60 14951.0 61 12358.5 62 10675.5 63 8680.5 64 6333.0 65 4744.0 66 3464.0 67 2682.5 68 1983.5 69 1320.5 70 1019.5 71 868.0 72 781.5 73 726.5 74 610.0 75 520.5 76 409.5 77 270.5 78 188.5 79 137.0 80 100.0 81 79.0 82 62.5 83 39.0 84 21.0 85 13.5 86 7.5 87 3.5 88 1.5 89 2.5 90 3.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0658891991458808 2 0.009855222094469344 3 0.004223666611915433 4 0.012670999835746299 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004106342539362226 10-11 5.866203627660323E-5 12-13 5.866203627660323E-5 14-15 5.866203627660323E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011732407255320646 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.003343736067766384 34-35 0.0010559166529788583 36-37 0.0021704953422343196 38-39 5.866203627660323E-5 40-41 0.0029331018138301617 42-43 0.011145786892554615 44-45 0.0016425370157448905 46-47 0.0018771851608513034 48-49 0.010383180420958772 50-51 2.3464814510641292E-4 52-53 0.0014665509069150809 54-55 0.010793814674894996 56-57 0.003578384212872797 58-59 8.799305441490485E-4 60-61 0.00563155548255391 62-63 0.0031677499589365748 64-65 0.018067907173193797 66-67 0.002815777741276955 68-69 1.1732407255320646E-4 70-71 0.0012905647980852712 72-73 5.866203627660323E-5 74-75 0.0 76-77 0.0013492268343618745 78-79 0.011380435037661028 80-81 0.016249384048619094 82-83 0.012201703545533472 84-85 0.007684726752235024 86-87 0.010676490602341788 88-89 0.030797569045216695 90-91 0.026339254288194853 92-93 0.002522467559893939 94-95 0.008271347115001056 96-97 0.010793814674894996 98-99 0.011204448928831217 100-101 0.01126311096510782 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 852340.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.507644576226774 #Duplication Level Percentage of deduplicated Percentage of total 1 79.12941789078552 42.34028768023872 2 11.869937467928565 12.702647903519178 3 3.7384100009601275 6.001005408347583 4 1.6169461393928992 3.4607591730214917 5 0.9191949988640072 2.4591979647730238 6 0.5346718139955839 1.7165417632921283 7 0.362112525349674 1.3563051812107185 8 0.2568675596993125 1.099550247004282 9 0.2111643195696818 1.0169014822843772 >10 1.2054983121002028 12.17125921505998 >50 0.10105842946056327 3.7554112777165067 >100 0.046997884775457875 4.796317411856186 >500 0.003751004886103944 1.2886598039185553 >1k 0.003309710193621127 3.2093119056269437 >5k 6.619420387242254E-4 2.6258435821303334 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8430 0.9890419316235305 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7511 0.8812211089471337 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6300 0.7391416570852007 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4301 0.504610836051341 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2690 0.31560175516812544 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2554 0.2996456813008893 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1832 0.21493770091747427 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1719 0.2016800807189619 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1711 0.20074148813853626 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1605 0.1883051364478964 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1565 0.18361217354576811 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1521 0.17844991435342702 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1491 0.17493019217683084 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1479 0.17352230330619237 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1276 0.14970551657789144 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1209 0.14184480371682662 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1201 0.14090621113640095 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1029 0.12072647065724945 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1732407255320646E-4 2 0.0 0.0 0.0 0.0 1.1732407255320646E-4 3 0.0 0.0 0.0 0.0 1.1732407255320646E-4 4 0.0 0.0 0.0 0.0 1.1732407255320646E-4 5 0.0 0.0 0.0 0.0 1.1732407255320646E-4 6 0.0 0.0 0.0 0.0 2.3464814510641292E-4 7 0.0 0.0 0.0 0.0 2.3464814510641292E-4 8 0.0 0.0 0.0 0.0 2.3464814510641292E-4 9 0.0 0.0 0.0 0.0 2.3464814510641292E-4 10-11 0.0 0.0 0.0 0.0 2.3464814510641292E-4 12-13 0.0 0.0 0.0 0.0 3.519722176596194E-4 14-15 0.0 0.0 0.0 0.0 4.1063425393622263E-4 16-17 0.0 0.0 0.0 0.0 4.6929629021282584E-4 18-19 0.0 0.0 0.0 0.0 5.866203627660323E-4 20-21 0.0 0.0 0.0 0.0 5.866203627660323E-4 22-23 0.0 0.0 0.0 2.3464814510641292E-4 5.866203627660323E-4 24-25 0.0 0.0 0.0 2.3464814510641292E-4 5.866203627660323E-4 26-27 0.0 0.0 0.0 4.1063425393622263E-4 5.866203627660323E-4 28-29 0.0 0.0 0.0 0.0011732407255320646 5.866203627660323E-4 30-31 0.0 0.0 0.0 0.0023464814510641293 5.866203627660323E-4 32-33 0.0 0.0 0.0 0.004868949010958069 6.452823990426356E-4 34-35 0.0 0.0 0.0 0.007156768425745594 7.039444353192388E-4 36-37 0.0 0.0 0.0 0.00979656005819274 7.039444353192388E-4 38-39 0.0 0.0 0.0 0.013902902597554966 7.039444353192388E-4 40-41 0.0 0.0 0.0 0.01976910622521529 7.039444353192388E-4 42-43 0.0 0.0 0.0 0.030386934791280476 7.039444353192388E-4 44-45 0.0 0.0 0.0 0.04640167069479316 7.626064715958421E-4 46-47 0.0 0.0 0.0 0.06488021212192317 8.799305441490484E-4 48-49 0.0 0.0 0.0 0.10218926719384283 9.385925804256517E-4 50-51 0.0 0.0 0.0 0.14213811389820963 0.0010559166529788581 52-53 0.0 0.0 0.0 0.18108970598587418 0.0010559166529788581 54-55 0.0 0.0 0.0 0.23693596452120047 0.0010559166529788581 56-57 0.0 0.0 0.0 0.30205082478823003 0.0012905647980852712 58-59 0.0 0.0 0.0 0.38746274960696436 0.0012905647980852712 60-61 0.0 0.0 0.0 0.4886547621841049 0.0012905647980852712 62-63 0.0 0.0 0.0 0.6176525799563555 0.0012905647980852712 64-65 0.0 0.0 0.0 0.7304010136799869 0.0012905647980852712 66-67 0.0 0.0 0.0 0.8716005349977709 0.0012905647980852712 68-69 0.0 0.0 0.0 1.062604125114391 0.0012905647980852712 70-71 0.0 0.0 0.0 1.2160053499777084 0.0012905647980852712 72-73 0.0 0.0 0.0 1.4035478799540089 0.0014078888706384775 74-75 0.0 0.0 0.0 1.6213600206490368 0.001525212943191684 76-77 0.0 0.0 0.0 1.819872351409062 0.001525212943191684 78-79 0.0 0.0 0.0 2.040382945772814 0.0016425370157448905 80-81 0.0 0.0 0.0 2.3219607199005092 0.0016425370157448905 82-83 0.0 0.0 0.0 2.6718797662904477 0.0016425370157448905 84-85 0.0 0.0 0.0 3.0386934791280473 0.0016425370157448905 86-87 0.0 0.0 0.0 3.439003214679588 0.0016425370157448905 88-89 0.0 0.0 0.0 3.8790271487903887 0.0016425370157448905 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTGC 145 6.6393113E-10 32.75668 8 ATACGTC 95 3.4912093E-5 29.99822 5 TGCACCG 145 7.887629E-7 26.205343 5 GGACCGT 100 0.0016538665 23.748592 6 GAGTACT 6500 0.0 23.525232 12-13 GTACATG 6785 0.0 23.349033 1 ACACCGT 125 2.256144E-4 22.798649 6 AGGCGAC 210 3.4457116E-8 22.617706 5 TACATGG 6920 0.0 22.447113 2 GTACTTT 6775 0.0 22.3951 14-15 AGGTAAG 300 7.2759576E-12 22.167952 2 GCGACAG 195 4.2212014E-7 21.921778 7 TGCGATA 55 0.0028813723 21.586462 56-57 AGTACTT 6555 0.0 21.516676 12-13 GCACCGT 200 5.385791E-7 21.373734 6 ACATGGG 6895 0.0 21.082954 3 ACTTTTT 7810 0.0 19.761675 16-17 AGAGTAC 11345 0.0 19.38063 10-11 TACTTTT 7280 0.0 19.341259 14-15 AGTGCGT 125 0.0060319575 18.998875 8 >>END_MODULE