FastQCFastQC Report
Thu 26 May 2016
SRR936413_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936413_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909090
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74970.8246708246708246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59530.6548306548306548No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT57010.6271106271106272No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT44570.4902704902704903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37230.40953040953040953No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34030.3743303743303743No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27830.3061303061303061No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT20150.22165022165022163No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16970.18667018667018667No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC15990.1758901758901759No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14380.15818015818015818No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14250.15675015675015674No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC13680.15048015048015048No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG11930.13123013123013122No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA10920.12012012012012012No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9770.10747010747010748No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT9470.10417010417010417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG71900.027.292991
TACATGG73100.026.519952
ACTGCGC908.9354586E-426.3942688
ACATGGG74150.024.8628273
GAGTACT57300.024.75253312-13
GTAAGCG701.7800638E-523.75026994-95
CATGGGA47700.022.9120164
GTACTTT61250.022.88473314-15
AGTACTT58500.022.37668212-13
ATGGGAG17350.021.3623475
TAAGGTG3351.8189894E-1221.2765055
GTATCAA145000.020.906561
ATGGGAT16050.020.4280955
ACTTTTT70650.020.07530416-17
GTATACT1908.2218685E-620.0073814
TACTTTT64600.019.96950314-15
CATGGGG22650.019.9300884
AGAGTAC103850.019.78617310-11
GATGTGC3404.1836756E-1119.562818
TATATAC4150.019.463943
Produced by FastQC (version 0.11.5)