##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936413_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 909090 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.64935484935485 31.0 31.0 34.0 30.0 34.0 2 31.69043109043109 33.0 31.0 34.0 30.0 34.0 3 31.713487113487112 33.0 31.0 34.0 30.0 34.0 4 35.368893068893065 37.0 35.0 37.0 33.0 37.0 5 35.364630564630566 37.0 35.0 37.0 33.0 37.0 6 35.493472593472596 37.0 35.0 37.0 33.0 37.0 7 35.37885027885028 37.0 35.0 37.0 33.0 37.0 8 35.34288574288574 37.0 35.0 37.0 33.0 37.0 9 36.864244464244464 39.0 37.0 39.0 33.0 39.0 10-11 36.77186472186472 39.0 37.0 39.0 32.5 39.0 12-13 36.67397782397782 39.0 37.0 39.0 32.0 39.0 14-15 37.714243914243916 40.0 37.0 41.0 32.5 41.0 16-17 37.62766612766613 40.0 37.0 41.0 32.0 41.0 18-19 37.665065615065615 40.0 37.0 41.0 32.0 41.0 20-21 37.612095612095615 40.0 37.0 41.0 32.0 41.0 22-23 37.464220814220816 40.0 37.0 41.0 32.0 41.0 24-25 37.555693605693605 40.0 37.0 41.0 32.0 41.0 26-27 37.51265276265276 40.0 37.0 41.0 32.0 41.0 28-29 37.40902605902606 40.0 37.0 41.0 32.0 41.0 30-31 37.17337722337722 39.0 36.5 41.0 31.0 41.0 32-33 36.92305327305327 39.0 35.5 41.0 31.0 41.0 34-35 36.65446765446765 39.0 35.0 41.0 30.5 41.0 36-37 36.44233189233189 39.0 35.0 40.5 30.0 41.0 38-39 36.208285758285754 39.0 35.0 40.0 30.0 41.0 40-41 36.00686400686401 38.5 35.0 40.0 30.0 41.0 42-43 35.70273735273736 38.0 35.0 40.0 28.5 41.0 44-45 35.32707047707048 38.0 35.0 40.0 27.5 41.0 46-47 34.85954800954801 38.0 34.0 40.0 24.5 41.0 48-49 34.61301906301906 38.0 34.0 40.0 23.5 41.0 50-51 33.63653598653599 37.5 33.0 39.5 22.5 40.5 52-53 33.77203027203027 38.0 33.5 39.5 22.5 40.5 54-55 34.16806696806697 38.0 34.0 40.0 22.0 41.0 56-57 34.04306834306834 38.0 34.0 40.0 21.5 41.0 58-59 33.78082643082644 38.0 33.5 40.0 20.5 41.0 60-61 33.43063118063118 37.0 33.0 40.0 19.5 41.0 62-63 33.127039677039676 37.0 33.0 40.0 18.0 41.0 64-65 32.78921998921999 36.0 32.5 39.5 16.0 41.0 66-67 32.38190278190278 36.0 32.0 39.0 12.5 41.0 68-69 31.975163075163074 35.0 31.5 39.0 8.0 40.5 70-71 31.51049621049621 35.0 31.0 38.0 7.0 40.0 72-73 31.128471878471878 35.0 31.0 37.0 6.0 39.5 74-75 30.725733975733974 35.0 31.0 37.0 2.0 39.0 76-77 30.301694001694003 35.0 30.0 36.0 2.0 39.0 78-79 29.864418264418262 34.0 30.0 36.0 2.0 38.5 80-81 29.40050105050105 34.0 29.0 35.5 2.0 37.0 82-83 28.928832678832677 34.0 29.0 35.0 2.0 37.0 84-85 28.600906950906953 34.0 29.0 35.0 2.0 36.0 86-87 28.325413325413326 34.0 29.0 35.0 2.0 36.0 88-89 27.915050765050765 34.0 27.0 35.0 2.0 36.0 90-91 27.485840235840236 34.0 26.5 35.0 2.0 35.0 92-93 27.143616143616143 33.5 26.0 35.0 2.0 35.0 94-95 26.69207834207834 33.0 25.0 35.0 2.0 35.0 96-97 26.12005412005412 33.0 24.5 35.0 2.0 35.0 98-99 25.465510565510563 33.0 21.0 35.0 2.0 35.0 100-101 23.11243496243496 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3555700788901852 1101 2 0.37645219024184584 1101 3 0.38826240172891957 1101 4 0.45082278123298636 1101 5 0.3758822570336591 1101 6 0.22676502160243217 1101 7 0.22248671476619108 1101 8 0.3897695808621151 1101 9 0.6438388956377281 1101 10-11 0.5109000895255207 1101 12-13 0.7202916665016232 1101 14-15 0.7344990611672344 1101 16-17 0.8438715883089358 1101 18-19 0.68131491692926 1101 20-21 0.6287154056942938 1101 22-23 0.6738304549433494 1101 24-25 0.7092793214618922 1101 26-27 0.6825789190831273 1101 28-29 0.707504676915562 1101 30-31 0.6888750761898663 1101 32-33 0.5277834669689341 1101 34-35 0.5524883260768618 1101 36-37 0.584742502032249 1101 38-39 0.544422008932365 1101 40-41 0.5297684873308057 1101 42-43 0.4682237323172913 1101 44-45 0.595916472917537 1101 46-47 0.6793166278035798 1101 48-49 0.7365254326916428 1101 50-51 0.602781743588892 1101 52-53 0.7684599340819389 1101 54-55 0.6595735505722189 1101 56-57 0.6745997756163362 1101 58-59 0.8126158593978516 1101 60-61 0.8341959720268903 1101 62-63 0.8528656882946919 1101 64-65 1.0190040942316472 1101 66-67 0.9198412337294712 1101 68-69 0.7981078430622155 1101 70-71 0.7403803312971782 1101 72-73 0.6771455733958192 1101 74-75 0.5688365924981724 1101 76-77 0.6987355126323926 1101 78-79 0.5937105359879169 1101 80-81 0.4829611270134144 1101 82-83 0.6858201774487505 1101 84-85 0.6868043062567715 1101 86-87 0.7039429525179024 1101 88-89 0.5550870029490085 1101 90-91 0.31221500723749784 1101 92-93 0.341501989053814 1101 94-95 0.3133804720459068 1101 96-97 0.4195929050380407 1101 98-99 0.595400017693958 1101 100-101 1.1473434387158115 1102 1 0.2954939618341186 1102 2 0.29301608612707497 1102 3 0.2206113678171704 1102 4 0.3463379455038975 1102 5 0.3050930470090307 1102 6 0.18114392660222478 1102 7 0.21662312710633813 1102 8 0.01584323837710855 1102 9 0.3176339988201704 1102 10-11 0.29733182421514215 1102 12-13 0.26365695009679513 1102 14-15 0.17876084226459454 1102 16-17 0.2177959739454991 1102 18-19 0.37863286044679256 1102 20-21 0.3559796532904116 1102 22-23 0.15586745590351114 1102 24-25 0.046368861628600655 1102 26-27 0.15319966619916414 1102 28-29 0.23867227185315443 1102 30-31 0.1800127269976599 1102 32-33 0.036128773315731166 1102 34-35 -0.1923164587219972 1102 36-37 0.11524498592520871 1102 38-39 -0.042167012886928035 1102 40-41 -0.064363967826921 1102 42-43 0.09467223502382893 1102 44-45 -0.2551821452865468 1102 46-47 0.06779822647048661 1102 48-49 0.48187965704909885 1102 50-51 0.5711088468965784 1102 52-53 0.5342260445977871 1102 54-55 0.4969013913943314 1102 56-57 0.466448284236904 1102 58-59 0.5163440035749645 1102 60-61 0.44375687262942876 1102 62-63 0.6186298419620897 1102 64-65 0.5274722471754458 1102 66-67 0.5919556215229136 1102 68-69 0.35158851113896716 1102 70-71 0.436585697758364 1102 72-73 0.3149681489910492 1102 74-75 -0.024069788393244806 1102 76-77 0.14307621995048692 1102 78-79 0.2691850499937303 1102 80-81 0.36077333566042213 1102 82-83 0.32128453323684525 1102 84-85 0.3521312564954151 1102 86-87 0.3487066869848725 1102 88-89 0.17468610123324524 1102 90-91 0.30711002449255176 1102 92-93 0.31891626169759846 1102 94-95 0.5010424556118593 1102 96-97 0.36295910182915847 1102 98-99 0.4689669523473974 1102 100-101 0.7718174813385801 1103 1 0.16070334458729718 1103 2 0.24020849647216025 1103 3 0.07400725910353856 1103 4 0.24617113473778574 1103 5 0.31569187416488376 1103 6 0.33853857738036197 1103 7 0.17198926499070666 1103 8 0.19768717868235086 1103 9 0.47086965779318035 1103 10-11 0.4231245277712645 1103 12-13 0.35164821955022063 1103 14-15 0.33216260716949364 1103 16-17 0.4913367321591693 1103 18-19 0.11964269993254817 1103 20-21 0.2584063975201687 1103 22-23 0.29109225103169933 1103 24-25 0.40424820340741263 1103 26-27 0.334911097670485 1103 28-29 -0.03156610308106167 1103 30-31 0.05030255483014656 1103 32-33 0.24050015516672119 1103 34-35 0.24863675908117955 1103 36-37 0.4342360392294822 1103 38-39 0.3076104944433453 1103 40-41 0.09037416276476051 1103 42-43 0.34072845260145357 1103 44-45 0.37751464442851557 1103 46-47 0.6198537580094694 1103 48-49 0.5902909137215318 1103 50-51 0.5921808057718465 1103 52-53 0.7032922802191663 1103 54-55 0.752008385229388 1103 56-57 0.9620818362254795 1103 58-59 0.7878501403315994 1103 60-61 0.7760157253472713 1103 62-63 0.5989111647949557 1103 64-65 0.7964151368078021 1103 66-67 0.71900856866975 1103 68-69 0.891664915168743 1103 70-71 1.0601990538969979 1103 72-73 0.9967641605691249 1103 74-75 0.8834554916608894 1103 76-77 0.8461384897046393 1103 78-79 1.0729234907124763 1103 80-81 0.9327185841379837 1103 82-83 0.6673092682191779 1103 84-85 0.6940211989809448 1103 86-87 0.7256280650767444 1103 88-89 0.6588038367418534 1103 90-91 0.7201788257105086 1103 92-93 0.8664077566979742 1103 94-95 0.9492185795875017 1103 96-97 0.860675438946668 1103 98-99 0.8069135823000302 1103 100-101 1.1204691334699994 1104 1 0.14740612149703836 1104 2 0.27270290766420047 1104 3 0.33897097525352393 1104 4 0.3277113754905443 1104 5 0.33657171412599496 1104 6 0.22735428426774718 1104 7 0.27719026176161776 1104 8 0.27538242639273847 1104 9 0.5350828577963824 1104 10-11 0.4574284574310781 1104 12-13 0.3074150413947834 1104 14-15 0.4628274683240079 1104 16-17 0.3940076081263584 1104 18-19 0.4558979715739824 1104 20-21 0.6264453602587778 1104 22-23 0.4152252179208489 1104 24-25 0.5253769690086685 1104 26-27 0.3209493606395881 1104 28-29 0.4796881669818873 1104 30-31 0.506690088249492 1104 32-33 0.3444599958756811 1104 34-35 0.3072608840354363 1104 36-37 0.3102724058766526 1104 38-39 0.22540894480111007 1104 40-41 0.19551319521323762 1104 42-43 0.37910146179728343 1104 44-45 0.4215921105150642 1104 46-47 0.5078706058950715 1104 48-49 0.3727197549085801 1104 50-51 0.1960236621134399 1104 52-53 0.5205602946098438 1104 54-55 0.6934136399107658 1104 56-57 0.6970841999507087 1104 58-59 0.5142445379021723 1104 60-61 0.4800038773414812 1104 62-63 0.5720830169673121 1104 64-65 0.35368699725987796 1104 66-67 0.5658629444993863 1104 68-69 0.4407259687224787 1104 70-71 0.48848710252830685 1104 72-73 0.5223380650425753 1104 74-75 0.37613435354722213 1104 76-77 0.5917185906103342 1104 78-79 0.5436036826950392 1104 80-81 0.6891830387851705 1104 82-83 0.7302981386790215 1104 84-85 0.8011884946389003 1104 86-87 0.8872318192600055 1104 88-89 0.8617890748103818 1104 90-91 0.8427170125596497 1104 92-93 0.7577196642642967 1104 94-95 0.6753664553062961 1104 96-97 0.7448382128116116 1104 98-99 1.218015345345254 1104 100-101 1.515674376933699 1105 1 0.20391960582941238 1105 2 0.1427285298533718 1105 3 0.07808187666218203 1105 4 0.06937852327835259 1105 5 0.20407825239636423 1105 6 0.11879278338215471 1105 7 0.15139466989302974 1105 8 0.3095154826002968 1105 9 0.415436989498815 1105 10-11 0.275760433670996 1105 12-13 0.05133084984088754 1105 14-15 -0.07204385997513896 1105 16-17 0.02882637808873767 1105 18-19 -0.0018698785179580568 1105 20-21 0.07337272158473951 1105 22-23 -0.015155557938420827 1105 24-25 0.1599638054015955 1105 26-27 0.11812959259970057 1105 28-29 -0.15521123224711886 1105 30-31 -0.10518285329860788 1105 32-33 -0.07590317426799942 1105 34-35 -0.2283789108543246 1105 36-37 -0.15313152949517672 1105 38-39 -0.1803244928525558 1105 40-41 -0.4594091385627337 1105 42-43 -0.2770920272069475 1105 44-45 -0.5536148877071128 1105 46-47 -0.4420085165665171 1105 48-49 -0.3860391383668116 1105 50-51 -0.3852974235347517 1105 52-53 -0.5344278698101519 1105 54-55 -0.5452370442434926 1105 56-57 -0.5188449577820364 1105 58-59 -0.155469118704751 1105 60-61 -0.35512652639546616 1105 62-63 -0.44365329414087284 1105 64-65 -0.2217481519241815 1105 66-67 -0.22641283811880086 1105 68-69 -0.14808633895349388 1105 70-71 -0.18442318928327595 1105 72-73 -0.1963852451770336 1105 74-75 -0.10430359277558665 1105 76-77 -0.30951785753442707 1105 78-79 -0.21932437409981986 1105 80-81 -0.4128411871572766 1105 82-83 -0.16543627448923104 1105 84-85 0.012587482094581048 1105 86-87 -0.02994234725568745 1105 88-89 -0.06011766466816226 1105 90-91 -0.18776317288102717 1105 92-93 0.0284946409216289 1105 94-95 0.025233433606739908 1105 96-97 0.14490902057086785 1105 98-99 0.16614351867097454 1105 100-101 0.36240909071971217 1106 1 0.1753774216865729 1106 2 0.18571695750524242 1106 3 0.2916360390456525 1106 4 0.24718326251447564 1106 5 0.2118475642762192 1106 6 0.24751100133759962 1106 7 0.2618733142120817 1106 8 0.24658537993596497 1106 9 0.5223958399406641 1106 10-11 0.4450965788393262 1106 12-13 0.38550655717474314 1106 14-15 0.58238367718387 1106 16-17 0.584761452936192 1106 18-19 0.4469488419500536 1106 20-21 0.3912721396584189 1106 22-23 0.5076503063258357 1106 24-25 0.37952032372631805 1106 26-27 0.3824970841399278 1106 28-29 0.6175877936108805 1106 30-31 0.4180139030908876 1106 32-33 0.28422229945707755 1106 34-35 0.26848689314371654 1106 36-37 0.18921055846877977 1106 38-39 0.25984871736005033 1106 40-41 0.34734985394149476 1106 42-43 0.25213708587381944 1106 44-45 0.3482606003565678 1106 46-47 0.05620335049486869 1106 48-49 -0.07011535791935586 1106 50-51 -0.036098399441499396 1106 52-53 0.09404107720764188 1106 54-55 0.19045325977880623 1106 56-57 0.07568696433954614 1106 58-59 0.11944628359206888 1106 60-61 0.15818807117535272 1106 62-63 0.3520318921064245 1106 64-65 0.32533890760524997 1106 66-67 0.3186054932218383 1106 68-69 0.385233464629831 1106 70-71 0.4998815653944142 1106 72-73 0.6131766115164403 1106 74-75 0.21707944180027638 1106 76-77 0.30046489203430937 1106 78-79 0.35957583369949475 1106 80-81 0.5749846849601106 1106 82-83 0.595279508111215 1106 84-85 0.7097802060303273 1106 86-87 0.7474189985426598 1106 88-89 0.8820153004206723 1106 90-91 0.7611398143995345 1106 92-93 0.8482995478090025 1106 94-95 0.8051430784066866 1106 96-97 1.068190043772919 1106 98-99 1.0453318219079648 1106 100-101 1.3846131752335857 1107 1 0.2453624415015021 1107 2 0.2963869575553417 1107 3 0.2804895789285169 1107 4 0.4106085644979771 1107 5 0.4684195568412335 1107 6 0.41890736501040493 1107 7 0.43414343851254955 1107 8 0.5219081553283829 1107 9 0.5685448301559504 1107 10-11 0.4528857740677097 1107 12-13 0.5312567438007889 1107 14-15 0.5289665416557909 1107 16-17 0.5710132503078995 1107 18-19 0.53752685664967 1107 20-21 0.6875451297334223 1107 22-23 0.7751823834612637 1107 24-25 0.7559147585124109 1107 26-27 0.6493568064804549 1107 28-29 0.7153933718510359 1107 30-31 0.7954138640121471 1107 32-33 0.561103715510427 1107 34-35 0.8009212483694981 1107 36-37 0.6902115755181413 1107 38-39 0.8149136732461599 1107 40-41 0.5918996217235701 1107 42-43 0.869795667916641 1107 44-45 1.001399174583625 1107 46-47 0.9213045293703033 1107 48-49 0.9209205794945277 1107 50-51 0.805890207601692 1107 52-53 0.8176576646313052 1107 54-55 0.7172367284682224 1107 56-57 0.8478608948094433 1107 58-59 1.0329209350146868 1107 60-61 0.7474389116188433 1107 62-63 0.784274861690136 1107 64-65 0.6970774607899202 1107 66-67 0.7919202224011315 1107 68-69 0.6268723670181409 1107 70-71 0.7902646873852284 1107 72-73 0.6618311504123149 1107 74-75 0.7475959325290944 1107 76-77 0.5411168056402254 1107 78-79 0.47855958596030135 1107 80-81 0.596191416524217 1107 82-83 0.8802326887702456 1107 84-85 0.7275545212755468 1107 86-87 0.8302308644524707 1107 88-89 0.4531857087935407 1107 90-91 0.46722708024020676 1107 92-93 0.37794628598643243 1107 94-95 0.28848100432741575 1107 96-97 0.4208200933227637 1107 98-99 0.9022114343269898 1107 100-101 1.2665623027254824 1108 1 -0.06985856215419872 1108 2 0.18106625300233503 1108 3 -0.0776651236304744 1108 4 0.23908147908753818 1108 5 0.22147289682129667 1108 6 0.12113401838361426 1108 7 -0.05152455749941964 1108 8 0.13405089377969404 1108 9 0.12695293916226547 1108 10-11 -0.09794091253912995 1108 12-13 0.2827107152198707 1108 14-15 0.17136227113402214 1108 16-17 0.12762466606064038 1108 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100-101 -5.462419647744177 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1968.0 3 710.0 4 674.0 5 794.0 6 832.0 7 965.0 8 1201.0 9 1509.0 10 1951.0 11 2622.0 12 3836.0 13 4774.0 14 5626.0 15 7499.0 16 9876.0 17 13772.0 18 11044.0 19 6513.0 20 6187.0 21 6246.0 22 6748.0 23 7426.0 24 8407.0 25 9455.0 26 11135.0 27 13010.0 28 15573.0 29 19047.0 30 23167.0 31 28952.0 32 37330.0 33 48600.0 34 66185.0 35 92090.0 36 126403.0 37 161792.0 38 125095.0 39 20068.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.17002922432309 18.34519471145382 14.64065965987113 20.844116404351958 2 15.51130024930742 20.763919297450375 39.839457464809804 23.8853229884324 3 19.995621220139306 26.301944376293417 29.920048760684274 23.782385642882996 4 12.090318633115219 16.404092026578805 38.05362421233111 33.45196512797487 5 12.734536210112749 38.66340910466569 33.28127837297984 15.32077631224172 6 29.997788065900227 36.14344337613774 17.807059921404807 16.05170863655722 7 27.541283091128193 34.58370246460466 20.352715247093265 17.522299197173876 8 27.106002183252343 32.00291393760124 19.871117684343968 21.01996619480245 9 27.47306195749776 16.681558885152384 21.018055531101997 34.82732362624786 10-11 25.224709672626826 26.402691838918862 26.240587230272734 22.132011258181578 12-13 27.381117449753056 24.629145427814315 26.96437375097941 21.02536337145322 14-15 23.707538761182697 25.100575503152612 27.647534579706633 23.544351155958054 16-17 21.154747703292205 28.859943743314563 27.65243889018501 22.332869663208214 18-19 20.95314660648971 26.811265240270355 31.269730430796777 20.965857722443154 20-21 22.737878881222276 24.886797683587975 30.954947898331337 21.420375536858412 22-23 21.84533953547731 25.254941783387775 31.282448761349848 21.617269919785066 24-25 22.052874315557304 25.31185285616459 31.372020560094825 21.26325226818328 26-27 21.495450480034588 25.198653020857055 32.050545821383345 21.25535067772501 28-29 20.78848275702749 26.343647178277674 31.612464619151915 21.255405445542923 30-31 21.056745332185574 25.444889674841242 31.509239371079218 21.98912562189397 32-33 20.941790682747094 25.78082416310115 31.402648133144783 21.87473702100697 34-35 22.08125233768234 25.562913907284766 30.959714857758907 21.39611889727399 36-37 21.914302954495827 25.080128866248735 31.50308936864449 21.50247881061095 38-39 21.493721658353486 25.63173956378342 31.318165716536228 21.556373061326866 40-41 21.358641787219344 26.01858808439769 31.212647553507566 21.410122574875395 42-43 21.198080896916135 26.21034342220983 30.13209646556565 22.459479215308384 44-45 21.38968166970365 25.83196151237829 30.29569181302021 22.482665004897854 46-47 22.350433814126802 26.411924044715168 29.657321214954557 21.580320926203473 48-49 22.244754171664855 26.484977386466202 29.015064139054946 22.255204302814 50-51 21.394769929016853 27.02700175670369 29.547547049848806 22.030681264430655 52-53 22.16712272258508 27.355764867652116 28.331715366105193 22.145397043657614 54-55 21.417916522741624 26.77440327027033 28.81557206629426 22.992108140693787 56-57 22.148786607104057 26.77561541520827 28.28525363341271 22.790344344274967 58-59 22.868997367428058 26.30481728762581 28.356804257879837 22.469381087066296 60-61 22.137214791329647 26.564910773367156 29.534199738065393 21.763674697237807 62-63 21.71859762486614 26.870771310723857 29.36102617751516 22.04960488689485 64-65 21.35129635129635 27.47852247852248 28.886468886468887 22.283712283712283 66-67 21.71399655682612 26.976552315891954 28.260592159904075 23.048858967377853 68-69 21.519047920282304 26.886405580018867 28.83622171553675 22.758324784162077 70-71 22.1599465810673 26.857974502800204 28.391898715239467 22.59018020089303 72-73 22.003177953866317 26.595810798993043 29.027182010735082 22.373829236405555 74-75 21.355875150979138 28.00660238090524 28.268189548393625 22.369332919722 76-77 21.634171132877473 27.118039971486652 28.297680650526708 22.95010824510917 78-79 21.72356950859112 27.215184651660884 28.443720611473854 22.61752522827415 80-81 22.08873602240705 27.4095310283449 27.96706708859576 22.53466586065229 82-83 21.900966681792553 27.08540626761822 28.476603000426277 22.537024050162945 84-85 21.63263060907353 27.40976662953642 28.069914875476158 22.887687885913884 86-87 21.746577226095585 27.499180470333023 27.86780381449808 22.886438489073306 88-89 21.50620374964111 27.489667750952375 27.414644127311348 23.589484372095175 90-91 21.613506647225414 27.04277533967649 27.910206492725752 23.43351152037234 92-93 22.076396143062478 27.42938491758984 27.926711806191584 22.5675071331561 94-95 21.66000743662914 27.110198522795027 27.887414275247686 23.342379765328147 96-97 21.136568403011164 27.128755567827668 28.528509367811605 23.206166661349567 98-99 21.44701207667946 27.67156580945879 27.30975502819065 23.571667085671095 100-101 21.277789759547883 27.160294810230766 27.645281407765065 23.916634022456282 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 358.0 1 282.5 2 344.5 3 498.5 4 431.5 5 418.5 6 657.0 7 1370.0 8 2331.0 9 2695.0 10 2310.0 11 1927.0 12 1873.0 13 1944.0 14 2062.0 15 2107.0 16 2144.0 17 2226.0 18 2310.5 19 2439.5 20 2623.5 21 2832.5 22 3194.0 23 3687.5 24 4182.0 25 4904.5 26 5835.0 27 6866.5 28 7986.0 29 9285.5 30 10890.0 31 12840.0 32 15165.5 33 16760.0 34 17886.0 35 18928.5 36 20747.0 37 22820.5 38 23069.0 39 22706.0 40 23091.5 41 24492.5 42 25443.0 43 26337.5 44 27525.5 45 28777.5 46 29918.0 47 34864.5 48 46282.5 49 45733.0 50 34690.0 51 29211.5 52 26985.0 53 26820.0 54 26768.5 55 25407.5 56 23516.5 57 21444.5 58 20118.5 59 18434.5 60 15982.5 61 13743.5 62 11745.5 63 9308.5 64 6789.0 65 5278.0 66 4009.0 67 3034.5 68 2200.0 69 1440.5 70 1083.5 71 877.5 72 765.0 73 770.0 74 651.0 75 497.0 76 391.5 77 278.5 78 188.0 79 128.0 80 94.0 81 62.0 82 41.0 83 30.5 84 19.0 85 13.5 86 10.0 87 9.0 88 7.0 89 4.0 90 4.0 91 2.5 92 3.0 93 3.0 94 1.0 95 0.5 96 0.0 97 0.5 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02838002838002838 2 0.01892001892001892 3 0.01771001771001771 4 0.04356004356004356 5 0.047850047850047844 6 0.04213004213004213 7 0.04675004675004675 8 0.03894003894003894 9 0.03718003718003718 10-11 0.045925045925045926 12-13 0.04158004158004158 14-15 0.0352000352000352 16-17 0.04378004378004378 18-19 0.048015048015048016 20-21 0.01078001078001078 22-23 0.01727001727001727 24-25 0.005225005225005225 26-27 0.01023001023001023 28-29 0.00583000583000583 30-31 0.007975007975007975 32-33 0.003795003795003795 34-35 0.007700007700007699 36-37 0.009405009405009405 38-39 0.01012001012001012 40-41 0.00143000143000143 42-43 0.002145002145002145 44-45 0.0020350020350020353 46-47 2.75000275000275E-4 48-49 0.001155001155001155 50-51 1.1000011000011E-4 52-53 0.003025003025003025 54-55 0.00572000572000572 56-57 0.014355014355014356 58-59 0.01023001023001023 60-61 0.009295009295009295 62-63 0.005115005115005115 64-65 0.0 66-67 0.004950004950004951 68-69 0.014575014575014575 70-71 0.00539000539000539 72-73 0.001595001595001595 74-75 0.00264000264000264 76-77 0.00462000462000462 78-79 0.0044550044550044545 80-81 0.009955009955009956 82-83 0.0057750057750057746 84-85 0.014025014025014024 86-87 0.00352000352000352 88-89 0.00473000473000473 90-91 0.002915002915002915 92-93 0.014355014355014356 94-95 0.00836000836000836 96-97 0.00803000803000803 98-99 0.020845020845020844 100-101 0.03322003322003322 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 909090.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.50877847905784 #Duplication Level Percentage of deduplicated Percentage of total 1 79.37528005584169 43.26649557277052 2 11.830040085494707 12.89682068837211 3 3.6620459554238636 5.988409552929874 4 1.6066664194541183 3.503096957910623 5 0.8857087966395172 2.4139452296488164 6 0.5217734007841468 1.7064738431764634 7 0.3638691987242913 1.388384588403027 8 0.2476539459295259 1.0799451262509676 9 0.19412535836502562 0.952338254065821 >10 1.1569501868197658 11.931974457681626 >50 0.10136873107366667 3.74143988335019 >100 0.048662242886490605 5.001925196687139 >500 0.002826852271891422 1.138051894765169 >1k 0.0024230162330497905 2.8828813616619025 >5k 6.057540582624476E-4 2.107817392325701 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7497 0.8246708246708246 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5953 0.6548306548306548 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5701 0.6271106271106272 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4457 0.4902704902704903 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3723 0.40953040953040953 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3403 0.3743303743303743 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2783 0.3061303061303061 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2015 0.22165022165022163 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1697 0.18667018667018667 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1599 0.1758901758901759 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1438 0.15818015818015818 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1425 0.15675015675015674 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1368 0.15048015048015048 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1193 0.13123013123013122 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1092 0.12012012012012012 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 977 0.10747010747010748 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 947 0.10417010417010417 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 5.5000055000055E-5 0.0 0.0 0.0 0.0 12-13 1.1000011000011E-4 0.0 0.0 0.0 0.0 14-15 1.1000011000011E-4 0.0 0.0 0.0 0.0 16-17 1.1000011000011E-4 0.0 0.0 0.0 0.0 18-19 1.1000011000011E-4 0.0 0.0 0.0 0.0 20-21 1.1000011000011E-4 0.0 0.0 0.0 2.2000022000022E-4 22-23 1.1000011000011E-4 0.0 0.0 0.0 3.3000033000033E-4 24-25 1.1000011000011E-4 0.0 0.0 0.0 3.3000033000033E-4 26-27 1.1000011000011E-4 0.0 0.0 1.65000165000165E-4 3.85000385000385E-4 28-29 1.1000011000011E-4 0.0 0.0 9.35000935000935E-4 4.4000044000044E-4 30-31 1.1000011000011E-4 0.0 0.0 0.00286000286000286 4.4000044000044E-4 32-33 1.1000011000011E-4 0.0 0.0 0.004950004950004951 4.4000044000044E-4 34-35 1.1000011000011E-4 0.0 0.0 0.007150007150007151 4.4000044000044E-4 36-37 2.2000022000022E-4 0.0 0.0 0.01034001034001034 4.4000044000044E-4 38-39 2.2000022000022E-4 0.0 0.0 0.01452001452001452 4.4000044000044E-4 40-41 2.2000022000022E-4 0.0 0.0 0.02057002057002057 4.4000044000044E-4 42-43 2.2000022000022E-4 0.0 0.0 0.0319000319000319 4.95000495000495E-4 44-45 2.2000022000022E-4 0.0 0.0 0.051425051425051424 5.5000055000055E-4 46-47 2.2000022000022E-4 0.0 0.0 0.07210507210507211 5.5000055000055E-4 48-49 2.2000022000022E-4 0.0 0.0 0.10879010879010878 5.5000055000055E-4 50-51 2.2000022000022E-4 0.0 0.0 0.14905014905014904 5.5000055000055E-4 52-53 2.2000022000022E-4 0.0 0.0 0.1890901890901891 6.6000066000066E-4 54-55 2.2000022000022E-4 0.0 0.0 0.2482702482702483 6.6000066000066E-4 56-57 2.2000022000022E-4 0.0 0.0 0.317020317020317 6.6000066000066E-4 58-59 2.2000022000022E-4 0.0 0.0 0.4008954008954009 6.6000066000066E-4 60-61 2.2000022000022E-4 0.0 0.0 0.5028655028655029 6.6000066000066E-4 62-63 2.2000022000022E-4 0.0 0.0 0.628045628045628 7.7000077000077E-4 64-65 2.2000022000022E-4 0.0 0.0 0.7383757383757383 8.8000088000088E-4 66-67 2.2000022000022E-4 0.0 0.0 0.8846758846758846 8.8000088000088E-4 68-69 2.2000022000022E-4 0.0 0.0 1.0825660825660828 8.8000088000088E-4 70-71 2.2000022000022E-4 0.0 0.0 1.2394262394262394 8.8000088000088E-4 72-73 2.2000022000022E-4 0.0 0.0 1.4346764346764347 9.35000935000935E-4 74-75 2.2000022000022E-4 0.0 0.0 1.6567116567116567 0.001045001045001045 76-77 2.2000022000022E-4 1.1000011000011E-4 0.0 1.8601568601568603 0.0011000011000011 78-79 2.2000022000022E-4 1.1000011000011E-4 0.0 2.073007073007073 0.00132000132000132 80-81 2.2000022000022E-4 1.1000011000011E-4 0.0 2.3586773586773586 0.00132000132000132 82-83 2.2000022000022E-4 1.1000011000011E-4 0.0 2.6941776941776943 0.00143000143000143 84-85 2.2000022000022E-4 1.1000011000011E-4 0.0 3.0595430595430595 0.00143000143000143 86-87 2.2000022000022E-4 1.1000011000011E-4 0.0 3.4590084590084587 0.00154000154000154 88-89 2.2000022000022E-4 1.1000011000011E-4 0.0 3.88998888998889 0.00165000165000165 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7190 0.0 27.29299 1 TACATGG 7310 0.0 26.51995 2 ACTGCGC 90 8.9354586E-4 26.394268 8 ACATGGG 7415 0.0 24.862827 3 GAGTACT 5730 0.0 24.752533 12-13 GTAAGCG 70 1.7800638E-5 23.750269 94-95 CATGGGA 4770 0.0 22.912016 4 GTACTTT 6125 0.0 22.884733 14-15 AGTACTT 5850 0.0 22.376682 12-13 ATGGGAG 1735 0.0 21.362347 5 TAAGGTG 335 1.8189894E-12 21.276505 5 GTATCAA 14500 0.0 20.90656 1 ATGGGAT 1605 0.0 20.428095 5 ACTTTTT 7065 0.0 20.075304 16-17 GTATACT 190 8.2218685E-6 20.007381 4 TACTTTT 6460 0.0 19.969503 14-15 CATGGGG 2265 0.0 19.930088 4 AGAGTAC 10385 0.0 19.786173 10-11 GATGTGC 340 4.1836756E-11 19.56281 8 TATATAC 415 0.0 19.46394 3 >>END_MODULE