FastQCFastQC Report
Thu 26 May 2016
SRR936413_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936413_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909090
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT86980.9567809567809569No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79560.8751608751608752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66850.7353507353507354No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45480.5002805002805003No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28100.3091003091003091No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT27060.29766029766029767No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC20550.22605022605022607No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT19800.21780021780021783No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT18110.19921019921019922No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC17850.19635019635019635No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC17180.18898018898018898No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17000.187000187000187No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG16360.17996017996017996No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA16060.17666017666017667No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA14260.15686015686015686No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12730.14003014003014003No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11950.13145013145013146No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC10330.11363011363011363No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC9200.1012001012001012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAAG3150.043.731022
GGTAAGG4150.034.3361363
TAAGGTG4450.034.1542175
GTAAGGT4300.033.136544
CGTATAG908.922112E-426.4010261
GCACCGT2355.456968E-1226.2756656
AAGGTAA5300.025.1058831
GTACATG67800.024.111381
GAGTACT69300.023.03392412-13
TACATGG70050.022.8519842
AGTACTT70150.022.82254812-13
GGTGTGC8150.022.729268
TGCACCG1903.2882417E-722.4979695
GTACTTT73050.021.91591614-15
ACATGGG69550.021.7174223
GTGTGCA7900.021.6471869
AAGGTGT7450.021.0395266
AGGTGTG7550.020.7608557
CATGGGG21850.020.4329384
TACTTTT78350.020.2515114-15
Produced by FastQC (version 0.11.5)