##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936413_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 909090 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.795736395736395 31.0 31.0 34.0 30.0 34.0 2 31.92139062139062 31.0 31.0 34.0 30.0 34.0 3 32.0002365002365 31.0 31.0 34.0 30.0 34.0 4 35.781581581581584 37.0 35.0 37.0 35.0 37.0 5 35.56192896192896 37.0 35.0 37.0 33.0 37.0 6 35.70439450439451 37.0 35.0 37.0 33.0 37.0 7 35.64101794101794 37.0 35.0 37.0 33.0 37.0 8 35.623496023496024 37.0 35.0 37.0 33.0 37.0 9 37.216849816849816 39.0 37.0 39.0 33.0 39.0 10-11 37.08030228030228 39.0 37.0 39.0 33.0 39.0 12-13 36.97740102740103 39.0 37.0 39.0 33.0 39.0 14-15 37.95544555544556 40.0 37.0 41.0 33.0 41.0 16-17 37.91730136730136 40.0 37.0 41.0 33.0 41.0 18-19 38.14008239008239 40.0 37.5 41.0 33.0 41.0 20-21 38.191140041140045 40.0 37.5 41.0 33.0 41.0 22-23 38.0993443993444 40.0 37.0 41.0 33.0 41.0 24-25 37.93431673431674 40.0 37.0 41.0 33.0 41.0 26-27 37.68012738012738 39.5 37.0 41.0 32.5 41.0 28-29 37.56910481910482 39.0 36.5 41.0 32.0 41.0 30-31 37.47690382690382 39.0 36.5 41.0 32.0 41.0 32-33 37.24167904167904 39.0 35.5 41.0 32.0 41.0 34-35 37.00063525063525 39.0 35.0 41.0 31.0 41.0 36-37 36.69238194238194 39.0 35.0 41.0 30.5 41.0 38-39 36.47542817542818 39.0 35.0 40.0 30.0 41.0 40-41 36.208296208296204 38.5 35.0 40.0 30.0 41.0 42-43 35.85692175692176 38.0 35.0 40.0 29.5 41.0 44-45 35.457246807246804 38.0 35.0 40.0 27.5 41.0 46-47 34.89570284570284 38.0 34.0 40.0 25.0 41.0 48-49 34.35843700843701 38.0 34.0 40.0 23.5 41.0 50-51 33.96666666666667 38.0 33.0 40.0 22.5 41.0 52-53 33.676920876920875 38.0 33.0 40.0 20.5 41.0 54-55 33.392434742434745 37.5 33.0 40.0 19.5 41.0 56-57 33.03348513348513 37.0 32.5 40.0 18.0 41.0 58-59 32.66636966636966 36.5 32.0 39.5 16.0 41.0 60-61 32.240979990979994 36.0 31.0 39.0 13.0 40.0 62-63 31.86014641014641 35.5 31.0 39.0 9.5 40.0 64-65 31.437541937541937 35.0 30.5 38.5 8.0 40.0 66-67 30.941793441793443 35.0 30.0 38.0 7.0 40.0 68-69 30.628594528594526 35.0 29.5 37.5 3.5 40.0 70-71 30.157707707707708 34.0 29.0 37.0 2.0 39.0 72-73 29.694447744447743 34.0 29.0 36.0 2.0 39.0 74-75 29.163793463793464 34.0 28.0 36.0 2.0 38.5 76-77 27.83911823911824 32.0 26.5 34.5 2.0 36.5 78-79 28.62160237160237 33.5 28.0 35.0 2.0 37.0 80-81 28.409856009856007 34.0 28.0 35.0 2.0 37.0 82-83 28.03880583880584 33.0 27.0 35.0 2.0 36.0 84-85 27.589032989032987 33.0 26.0 35.0 2.0 36.0 86-87 27.21765446765447 33.0 26.0 35.0 2.0 35.5 88-89 26.762492712492712 33.0 25.0 35.0 2.0 35.0 90-91 26.215109065109065 32.0 24.5 34.0 2.0 35.0 92-93 25.677278377278377 32.0 23.0 34.0 2.0 35.0 94-95 25.03676478676479 32.0 19.5 34.0 2.0 35.0 96-97 24.191265991265993 31.0 12.0 34.0 2.0 35.0 98-99 23.194471394471396 31.0 2.0 34.0 2.0 35.0 100-101 20.08918698918699 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32828297586479493 1101 2 0.39771399669671226 1101 3 0.3076524011720281 1101 4 0.30101220116187477 1101 5 0.13821008507681398 1101 6 0.16055926771786488 1101 7 0.14637307152391088 1101 8 0.14944339293192854 1101 9 0.20827742819795958 1101 10-11 0.29426914735302745 1101 12-13 0.27306190270648045 1101 14-15 0.4985222695252105 1101 16-17 0.42131453737688673 1101 18-19 0.3313863965924 1101 20-21 0.40791945816778963 1101 22-23 0.3242139585997137 1101 24-25 0.23676026782773363 1101 26-27 0.046500978905022805 1101 28-29 -0.027070171515966024 1101 30-31 0.3188564897286241 1101 32-33 0.41079009279965106 1101 34-35 0.3933679121211 1101 36-37 0.3386171626501451 1101 38-39 0.4443967878961885 1101 40-41 0.3237089479372557 1101 42-43 0.3417131690896582 1101 44-45 0.28432333304609614 1101 46-47 0.25701667979421927 1101 48-49 0.4671832881921034 1101 50-51 0.4401895379890206 1101 52-53 0.3903274134614563 1101 54-55 0.4759517249946299 1101 56-57 0.5224435757158332 1101 58-59 0.3352328911240363 1101 60-61 0.29864338567036697 1101 62-63 0.38202926854141595 1101 64-65 0.33469385541552654 1101 66-67 0.3922068423782221 1101 68-69 0.2290522173365197 1101 70-71 0.392977952792819 1101 72-73 0.3732659849798985 1101 74-75 0.443900489940777 1101 76-77 0.29769419369292294 1101 78-79 0.07683099869352716 1101 80-81 0.2706758140567125 1101 82-83 0.28191254913856767 1101 84-85 0.4419338188928421 1101 86-87 0.3159139357691849 1101 88-89 0.20815004463443998 1101 90-91 0.2730086955472508 1101 92-93 0.44715151275393694 1101 94-95 0.4411339639384977 1101 96-97 0.3978287621652008 1101 98-99 0.6517475834096693 1101 100-101 1.1034057000687802 1102 1 0.22391468133213976 1102 2 0.24137746050980624 1102 3 0.199101234368527 1102 4 0.14107723810717232 1102 5 0.09045124186125264 1102 6 0.1871963236437253 1102 7 0.20035034425118425 1102 8 0.26170300497150123 1102 9 0.16264676638311926 1102 10-11 0.30144491061088985 1102 12-13 0.19195336110649208 1102 14-15 0.2816154033353797 1102 16-17 0.3287090762912115 1102 18-19 0.31600578522076006 1102 20-21 0.11335984473668503 1102 22-23 0.08810671286825311 1102 24-25 0.1531804440658533 1102 26-27 -0.11517297013690353 1102 28-29 -0.02355915054550195 1102 30-31 0.023454779182166874 1102 32-33 0.15890695231009744 1102 34-35 -0.031888956851808814 1102 36-37 -0.020634773325248545 1102 38-39 0.02639395611905826 1102 40-41 0.08449748828486037 1102 42-43 0.0991557160192329 1102 44-45 0.05754764527909373 1102 46-47 0.06082414156174565 1102 48-49 -0.013406732054761505 1102 50-51 -0.052170055960573336 1102 52-53 -0.014169902612188423 1102 54-55 0.1899274496373593 1102 56-57 0.009465321955211436 1102 58-59 -0.014119131126641093 1102 60-61 0.10283112831957197 1102 62-63 0.03567513811267986 1102 64-65 0.3003433377638842 1102 66-67 0.1456430682524541 1102 68-69 -0.028641675904790276 1102 70-71 0.029584455791582087 1102 72-73 0.02069559931551268 1102 74-75 -0.1208668451273418 1102 76-77 -0.28972931527553314 1102 78-79 -0.23802678625839846 1102 80-81 0.0583392761303827 1102 82-83 -0.04509791695664234 1102 84-85 0.03552509674097237 1102 86-87 -0.014070939966590146 1102 88-89 -0.05426855047013035 1102 90-91 0.1259863231163756 1102 92-93 0.3963858631846193 1102 94-95 0.49582613863201175 1102 96-97 0.5547834450339941 1102 98-99 0.8766577423231574 1102 100-101 1.1658287306965036 1103 1 0.05174388590322465 1103 2 0.13465146246454296 1103 3 0.1600693946572278 1103 4 0.09118014390373474 1103 5 -0.059854656191525635 1103 6 0.06620119169875238 1103 7 -0.12365583949439696 1103 8 -0.05384751754559858 1103 9 0.10663359068204414 1103 10-11 -0.07162971723663958 1103 12-13 -0.06822561979719666 1103 14-15 -0.09865041314458267 1103 16-17 -0.03460806151797868 1103 18-19 -0.11808634131107709 1103 20-21 0.00594028981600303 1103 22-23 -0.06090276010370843 1103 24-25 -0.03090792335012793 1103 26-27 0.13344179567017278 1103 28-29 -0.0848656312612448 1103 30-31 -0.006031020757681915 1103 32-33 0.10203035468586563 1103 34-35 0.050672739637178665 1103 36-37 0.15502988534702666 1103 38-39 0.15724220061195382 1103 40-41 0.31127813447687913 1103 42-43 0.19608968714922526 1103 44-45 0.24672468084033738 1103 46-47 0.16738027455927096 1103 48-49 0.41234109705583677 1103 50-51 0.4310727453325569 1103 52-53 0.2875007894410473 1103 54-55 0.30727107041774815 1103 56-57 0.3565722124145694 1103 58-59 0.5280809384170126 1103 60-61 0.2612529575053557 1103 62-63 0.19141460319049486 1103 64-65 0.4388888418613206 1103 66-67 0.5068969213462076 1103 68-69 0.5112101209525903 1103 70-71 0.3906317924579952 1103 72-73 0.3071512699417802 1103 74-75 0.08270619449374195 1103 76-77 -0.08651174704758091 1103 78-79 0.02517710818563046 1103 80-81 0.3833578367649224 1103 82-83 0.5669469950800448 1103 84-85 0.2967122255282817 1103 86-87 0.22829875968005453 1103 88-89 0.18691350351077318 1103 90-91 0.10904069872723099 1103 92-93 0.35485256681592503 1103 94-95 0.59459831741556 1103 96-97 0.6633502351249625 1103 98-99 0.9330252146936537 1103 100-101 1.0753153294484754 1104 1 0.1291052741177161 1104 2 0.25589053524324257 1104 3 0.21112158624450217 1104 4 0.18443805169975036 1104 5 0.004609606152591539 1104 6 0.12375803461652879 1104 7 0.13489777433432693 1104 8 0.14112055213221453 1104 9 -0.012447414315168714 1104 10-11 0.118977346161941 1104 12-13 0.1428163156263551 1104 14-15 0.2587504506167022 1104 16-17 0.17661629473414564 1104 18-19 0.06933703793460921 1104 20-21 0.12407553763322454 1104 22-23 0.17628678395105624 1104 24-25 0.1971068946697372 1104 26-27 0.08517158849031858 1104 28-29 0.18575820523841458 1104 30-31 0.03237003263579652 1104 32-33 0.2276777082484145 1104 34-35 0.015596659735251706 1104 36-37 0.312574175147752 1104 38-39 0.36840859885558785 1104 40-41 0.17320399630178684 1104 42-43 0.2992605510974684 1104 44-45 0.38288567056013534 1104 46-47 0.5347141260425374 1104 48-49 0.4314063438848379 1104 50-51 0.23987755504600017 1104 52-53 0.5159063566149129 1104 54-55 0.49225754243097697 1104 56-57 0.4962614292870313 1104 58-59 0.485100134002586 1104 60-61 0.5532817281812683 1104 62-63 0.31346321847485115 1104 64-65 0.4245467720927003 1104 66-67 0.22513579915096216 1104 68-69 0.5606696692006281 1104 70-71 0.6285478147758354 1104 72-73 0.46279683401742844 1104 74-75 0.5786253631034697 1104 76-77 0.5749005053288307 1104 78-79 0.7057804056491008 1104 80-81 0.9882281790910952 1104 82-83 0.8284222801292849 1104 84-85 0.7551862472531852 1104 86-87 0.6330497138504931 1104 88-89 0.8125165205322666 1104 90-91 0.730799791056743 1104 92-93 0.5311298464753342 1104 94-95 0.793392416916376 1104 96-97 0.9596224935375552 1104 98-99 1.2805326196972722 1104 100-101 1.5313468351013846 1105 1 -0.02986511337392983 1105 2 0.016300171734656033 1105 3 0.0754567066940055 1105 4 0.09042565403753855 1105 5 -0.06349060242171589 1105 6 0.012156511751989285 1105 7 0.11356917656022603 1105 8 0.08384191928603713 1105 9 0.012136216815562761 1105 10-11 -0.06810080184684608 1105 12-13 -0.1243051266487214 1105 14-15 0.07037858445730194 1105 16-17 0.08071192967137364 1105 18-19 0.04335649646429118 1105 20-21 0.11514028857506275 1105 22-23 0.07202551061917006 1105 24-25 -0.08403275853814307 1105 26-27 0.014403646155848548 1105 28-29 0.06316422568971092 1105 30-31 0.03384060428070512 1105 32-33 -0.010178296508378537 1105 34-35 0.058383923894645307 1105 36-37 -0.020305245277128847 1105 38-39 0.08140324945214417 1105 40-41 0.13567123858842223 1105 42-43 0.09379395318135408 1105 44-45 0.37615661077701645 1105 46-47 0.04968761129070032 1105 48-49 -0.0019082754952535197 1105 50-51 0.14718011122602093 1105 52-53 -0.13256795848426606 1105 54-55 0.13581479949632325 1105 56-57 -0.06184174564794631 1105 58-59 0.31509515025120294 1105 60-61 -0.08504889428330387 1105 62-63 0.2475260641769772 1105 64-65 -0.044801829702912244 1105 66-67 0.2313834059880584 1105 68-69 -0.026713542790634648 1105 70-71 0.12749631623280422 1105 72-73 0.021600123160617102 1105 74-75 -0.15012683429290163 1105 76-77 -0.04599793696682397 1105 78-79 0.19665602621241618 1105 80-81 0.14431515782952076 1105 82-83 0.10583672849569581 1105 84-85 0.04226393073122381 1105 86-87 0.14880493799653394 1105 88-89 0.06577072436968834 1105 90-91 0.27827848402646893 1105 92-93 0.21889516819274135 1105 94-95 0.17801691348116933 1105 96-97 0.4587010636057194 1105 98-99 0.6163060667211901 1105 100-101 0.7271933329044877 1106 1 0.04486851967277872 1106 2 0.04533207400280048 1106 3 -0.05557774563789053 1106 4 0.04149354838195052 1106 5 0.02002530900548294 1106 6 -0.07810692130442476 1106 7 -0.020095635648310406 1106 8 0.001734278359720065 1106 9 0.158845178657252 1106 10-11 -0.009485071747711515 1106 12-13 -0.04161908890954891 1106 14-15 0.06472476664126248 1106 16-17 0.01316259387915153 1106 18-19 -0.08140647555118363 1106 20-21 -0.17469078073108335 1106 22-23 -0.017001273873972877 1106 24-25 -0.0660323085268999 1106 26-27 0.0686851502522714 1106 28-29 -0.12693640917910187 1106 30-31 -0.09681177967595289 1106 32-33 -0.11169906982183164 1106 34-35 -0.014636136900989527 1106 36-37 -0.14607280123073707 1106 38-39 0.07617076569886905 1106 40-41 -0.05256582027923429 1106 42-43 -0.12814100695734965 1106 44-45 -0.09335380345392963 1106 46-47 -0.7857688633564592 1106 48-49 -0.5657472244101527 1106 50-51 -0.47222110909860504 1106 52-53 -0.5949130465232315 1106 54-55 -0.34868716599399363 1106 56-57 -0.5425133212608628 1106 58-59 -0.5599102542956729 1106 60-61 -0.423401764869773 1106 62-63 -0.4851253403434299 1106 64-65 -0.36169357552596537 1106 66-67 -0.36687831526085546 1106 68-69 -0.3408469843619706 1106 70-71 -0.25340997113026376 1106 72-73 -0.21238102048561913 1106 74-75 -0.15215894391917217 1106 76-77 -0.7254200695789628 1106 78-79 -0.5204201359857556 1106 80-81 -0.25214298891826203 1106 82-83 0.08883203867256739 1106 84-85 -0.27917072278217603 1106 86-87 -0.14345222137362512 1106 88-89 -0.041330188447251004 1106 90-91 0.009834419983690168 1106 92-93 -0.08602319006033099 1106 94-95 0.32844067407518196 1106 96-97 0.01231946288922714 1106 98-99 0.4677855890033662 1106 100-101 0.6161768810226 1107 1 0.06574358292523286 1107 2 0.12702769067038489 1107 3 0.04120126406666458 1107 4 0.10423497235125012 1107 5 0.06765327936575716 1107 6 0.060965309240124554 1107 7 0.037245907561256786 1107 8 -7.847975782908634E-4 1107 9 0.15656859133379442 1107 10-11 0.054419696360028524 1107 12-13 0.14987978131395607 1107 14-15 0.2857250822772741 1107 16-17 0.20486186791633543 1107 18-19 0.1949093007380398 1107 20-21 0.21165670946165704 1107 22-23 0.1000310462510754 1107 24-25 0.06410491798452256 1107 26-27 0.09619558377581683 1107 28-29 -0.0037767424506398584 1107 30-31 0.07981462252448779 1107 32-33 0.22244472993467213 1107 34-35 -0.09466439383871972 1107 36-37 0.06674703073066723 1107 38-39 -0.10384697003863863 1107 40-41 0.002845834204556752 1107 42-43 -0.37402646615763757 1107 44-45 0.01921251768316523 1107 46-47 -0.017208714656831603 1107 48-49 -0.4328840050230838 1107 50-51 -0.24518012150861068 1107 52-53 -0.2928408710897301 1107 54-55 -0.38500334309084394 1107 56-57 -0.8349701217137806 1107 58-59 -0.6523755719106674 1107 60-61 -0.48813069366129014 1107 62-63 -0.48052857786704095 1107 64-65 -0.34308608142535846 1107 66-67 -0.2810568850790389 1107 68-69 -0.290176884109961 1107 70-71 -0.4155982805737928 1107 72-73 -0.16128406011046437 1107 74-75 -0.20326592448501302 1107 76-77 -0.2847220630959022 1107 78-79 -0.34080621393281874 1107 80-81 -0.32445018792425984 1107 82-83 -0.25259543769230675 1107 84-85 -0.2665633188632768 1107 86-87 0.006507948368405181 1107 88-89 -0.04299253808176573 1107 90-91 -0.3742490081239467 1107 92-93 -0.5402825226154278 1107 94-95 -0.07957597948173145 1107 96-97 0.14929346550449552 1107 98-99 0.6340736594653471 1107 100-101 1.0940182313434974 1108 1 -0.007038072109661897 1108 2 0.034243543113056774 1108 3 -0.0027917568705255746 1108 4 -0.04328081362415759 1108 5 -0.05273555413374709 1108 6 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0.09340781857934033 2212 3 -0.023128916124470322 2212 4 0.08148006633758342 2212 5 0.23528453194946763 2212 6 0.13490325151087035 2212 7 0.15690522229996162 2212 8 0.037124644826199926 2212 9 -0.13267951799674904 2212 10-11 -0.009619892191395252 2212 12-13 -0.08549701512599484 2212 14-15 -0.16211675805539016 2212 16-17 -0.2726628749283009 2212 18-19 -0.20538919402158484 2212 20-21 -0.063813222206754 2212 22-23 -0.0010863333175308298 2212 24-25 -0.03483363221852187 2212 26-27 0.1928057532673435 2212 28-29 0.007804367876907747 2212 30-31 0.24071120108149557 2212 32-33 0.2471286051383359 2212 34-35 0.032918710506777416 2212 36-37 0.2923111919056822 2212 38-39 0.5412032451693918 2212 40-41 0.46662158013748467 2212 42-43 0.5634817409284167 2212 44-45 0.5809291969137647 2212 46-47 0.7020329505913239 2212 48-49 0.8721618330723828 2212 50-51 0.8177378319480937 2212 52-53 0.848422533817498 2212 54-55 0.6243916426835483 2212 56-57 0.8830933707357005 2212 58-59 0.9704212814317472 2212 60-61 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-0.2870390419784634 2213 82-83 -0.3047127308712163 2213 84-85 -0.25457484221034576 2213 86-87 -0.2043107114862508 2213 88-89 -0.08277065325802013 2213 90-91 0.00289056147755673 2213 92-93 0.03666463802803932 2213 94-95 0.29098025828742635 2213 96-97 0.6144998654332987 2213 98-99 -0.05459677980133648 2213 100-101 -0.019296447362147973 2214 1 -0.25536805459880085 2214 2 -0.19871173933067965 2214 3 -0.11485317460189393 2214 4 -0.17375025608691885 2214 5 -0.12052529406499701 2214 6 -0.17555335967045238 2214 7 -0.2777023018348146 2214 8 -0.3094090900811608 2214 9 -0.327243478520586 2214 10-11 -0.30558353540003935 2214 12-13 -0.22552982228978635 2214 14-15 -0.3451941775837213 2214 16-17 -0.29170850915532043 2214 18-19 -0.2953998160131448 2214 20-21 -0.27234442934903313 2214 22-23 -0.27523455715919454 2214 24-25 -0.40429473421653483 2214 26-27 -0.42086569105459404 2214 28-29 -0.4304872394052026 2214 30-31 -0.3258295255165109 2214 32-33 -0.30807012022268054 2214 34-35 -0.2285812760476631 2214 36-37 0.0038266408528926377 2214 38-39 -0.20648008502664084 2214 40-41 -0.3181190550307136 2214 42-43 0.09771816763667829 2214 44-45 0.18013658121473242 2214 46-47 0.0978870124946809 2214 48-49 0.12457262178887163 2214 50-51 0.2439385203607074 2214 52-53 0.19641847325869577 2214 54-55 -0.03868012971003054 2214 56-57 -0.2071485148694805 2214 58-59 -0.09500413605285729 2214 60-61 -0.22973663658225263 2214 62-63 -0.14798761201047483 2214 64-65 -0.5158242066307785 2214 66-67 -0.5232926914416502 2214 68-69 -0.22016695798975405 2214 70-71 -0.13662538668654278 2214 72-73 -0.2821194590954299 2214 74-75 -0.024415485724116337 2214 76-77 0.3145787115371981 2214 78-79 0.15027401835817855 2214 80-81 -0.10796953066780546 2214 82-83 -0.1041455283244872 2214 84-85 -0.2054083054031075 2214 86-87 0.2065912706177535 2214 88-89 -0.07899203682918099 2214 90-91 0.18082941701958433 2214 92-93 -0.3315102726618697 2214 94-95 -0.17746038579971213 2214 96-97 -0.4278505160019215 2214 98-99 -0.29864912037594493 2214 100-101 -0.7482372513814219 2215 1 -0.38895954485523987 2215 2 -0.2343904997461408 2215 3 -0.2898800640760477 2215 4 -0.2307478542301311 2215 5 -0.0844125281018151 2215 6 -0.2128306622585825 2215 7 -0.31072850660456197 2215 8 -0.3342660165131761 2215 9 -0.8075110523756592 2215 10-11 -0.7284761149834509 2215 12-13 -0.3848320977188777 2215 14-15 -0.6035831543819441 2215 16-17 -0.5378098723640434 2215 18-19 -0.5358099108204399 2215 20-21 -0.3375355670096596 2215 22-23 -0.4172709597114732 2215 24-25 -0.5309455053176961 2215 26-27 -0.7514485254612637 2215 28-29 -0.5343567627808525 2215 30-31 -0.4942998311804203 2215 32-33 -0.44015000420032635 2215 34-35 -0.42134510963753513 2215 36-37 -0.6395198654024412 2215 38-39 -0.8178193560590614 2215 40-41 -1.038092475560525 2215 42-43 -1.3197223857338756 2215 44-45 -0.9548317309889143 2215 46-47 -0.9203580685511312 2215 48-49 -1.0622081353216828 2215 50-51 -1.5162788058955528 2215 52-53 -0.9140871566828324 2215 54-55 -0.5861188230568075 2215 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100-101 -2.027348437879599 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 10.0 5 46.0 6 81.0 7 219.0 8 547.0 9 976.0 10 1637.0 11 2573.0 12 3782.0 13 5055.0 14 6681.0 15 9208.0 16 12012.0 17 15620.0 18 13075.0 19 8066.0 20 7125.0 21 7137.0 22 7546.0 23 8230.0 24 9582.0 25 11218.0 26 13596.0 27 16392.0 28 20196.0 29 24149.0 30 30190.0 31 36235.0 32 44516.0 33 56435.0 34 73116.0 35 97654.0 36 131258.0 37 153144.0 38 78287.0 39 3488.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.9144559911617 19.49542899649197 15.4439177263384 20.14619728600793 2 16.115703843044074 22.356979027440886 38.26983262654764 23.257484502967397 3 20.721776158528066 26.299827404701325 30.138725258865446 22.83967117790516 4 12.840685214946424 17.52571536057605 37.29170035431152 32.341899070166 5 12.769997051977594 39.45135983033471 32.75758895767608 15.021054160011616 6 30.274120274120275 37.26968726968727 16.584606584606583 15.871585871585872 7 27.788997788997786 34.980584980584986 19.77977977977978 17.45063745063745 8 27.665797665797665 32.48259248259248 18.97952897952898 20.872080872080872 9 27.463759985391796 17.018231658889558 20.36634920215867 35.15165915355998 10-11 25.68410631539868 26.968526131972997 25.63829114359179 21.709076409036534 12-13 27.875733845498917 24.957842040478244 26.601534956731026 20.564889157291816 14-15 23.635981627367325 25.70502035322932 27.522153146706607 23.136844872696752 16-17 21.07065775781083 29.112553874450253 27.638225503763238 22.178562863975674 18-19 20.884180884180882 26.945736945736947 31.548031548031545 20.622050622050622 20-21 22.753742753742753 24.776479776479775 31.554356554356556 20.915420915420917 22-23 21.82978597019621 25.263755955203017 31.78684140762244 21.119616666978338 24-25 22.321271918366133 25.449257411447903 31.82138934432316 20.408081325862806 26-27 21.642730598364125 25.257424343011714 32.55721472938611 20.542630329238047 28-29 20.72058544473504 26.759840603265488 31.952059974533924 20.56751397746555 30-31 21.21554290667469 25.480442665607722 31.839099488660832 21.464914939056754 32-33 21.10909932910859 25.95746951068597 31.75569168695254 21.177739473252892 34-35 22.110154467953798 25.44692188925809 31.67338358139256 20.769540061395553 36-37 22.22239946314336 25.05316310870797 31.824707506641953 20.89972992150672 38-39 21.556067094048828 25.8603059880256 31.808783610966724 20.77484330695885 40-41 21.543703524442293 26.011242134905622 31.44492233906807 21.000132001584017 42-43 21.415363529163294 26.3180242176775 30.49204257453624 21.77456967862297 44-45 21.916895057032963 25.814738974974137 30.370831153930983 21.897534814061917 46-47 22.718267718267718 26.749221749221746 29.62627462627463 20.906235906235906 48-49 22.67115248595569 26.90969071167006 28.97945961167633 21.43969719069792 50-51 21.717077549323243 27.326542948113996 29.506825310657646 21.44955419190511 52-53 22.48810459486321 27.75733868532604 28.187943826077106 21.566612893733648 54-55 21.660537631688083 27.118202188852013 28.763086305539453 22.45817387392045 56-57 22.579413859578203 27.068445383119794 28.28489163524763 22.06724912205438 58-59 23.33305099847539 26.45310784678237 28.222687402511976 21.99115375223026 60-61 22.214485292499944 26.985787516775574 29.513948474248124 21.28577871647636 62-63 21.92819328135528 27.0944818008058 29.339768711411796 21.637556206427128 64-65 21.565004639711418 27.98551815978099 28.6753967682086 21.77408043229899 66-67 22.124148855423677 27.104403401278233 28.171427942842687 22.60001980045541 68-69 22.028138956724526 27.23692605548589 28.45549248674455 22.279442501045036 70-71 22.665907665907667 27.22931722931723 28.269753269753267 21.835021835021834 72-73 22.455686260705807 26.834699907379502 28.825270656461544 21.884343175453147 74-75 21.862213453475192 28.489230080420313 27.817089852827692 21.831466613276802 76-77 22.184991903689102 27.345620071810757 27.99046483384962 22.47892319065052 78-79 22.20820279498733 27.622445701914955 27.927762426077162 22.241589077020556 80-81 22.472735153116563 27.737045210033372 27.75167593279108 22.03854370405898 82-83 22.37036857769415 27.39562300581322 28.10807124152342 22.125937174969213 84-85 21.870377999539073 27.86189318238947 27.834171492752485 22.43355732531898 86-87 22.025248086207828 27.861117925239178 27.30075825661481 22.81287573193819 88-89 21.75716751540434 27.83304015304781 27.025006683190373 23.384785648357475 90-91 22.23456840377441 27.28394885160342 27.33950666858827 23.1419760760339 92-93 22.532712999563845 27.865079055155185 27.344388093243666 22.257819852037304 94-95 21.981690129225118 27.592958757414838 27.329943431354486 23.09540768200556 96-97 21.49228562439912 27.52655976028565 28.00473444639757 22.976420168917656 98-99 21.81242365090706 28.033599376061876 26.69238541379627 23.461591559234797 100-101 21.288408481877667 27.716425167538645 26.97300680715125 24.02215954343244 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 717.0 1 535.5 2 446.5 3 558.0 4 490.5 5 442.5 6 739.5 7 1491.0 8 2318.0 9 2681.0 10 2408.5 11 2005.0 12 1926.0 13 2077.0 14 2244.0 15 2323.5 16 2414.0 17 2556.5 18 2677.0 19 2746.5 20 2884.5 21 3160.0 22 3568.0 23 4006.0 24 4521.5 25 5219.5 26 6153.0 27 7343.0 28 8324.5 29 9536.5 30 11106.5 31 12941.5 32 15265.5 33 16802.5 34 17716.0 35 18768.0 36 20597.5 37 22372.0 38 22878.0 39 22724.0 40 22949.0 41 24244.5 42 25182.0 43 26173.0 44 27459.0 45 28492.5 46 29066.5 47 34339.0 48 46635.0 49 45562.0 50 34001.5 51 28872.5 52 26683.0 53 26143.5 54 25920.0 55 24805.5 56 23068.0 57 21371.5 58 20324.0 59 18782.5 60 16244.5 61 13510.5 62 11549.5 63 9404.5 64 6824.5 65 5125.5 66 3848.5 67 3060.0 68 2324.5 69 1546.5 70 1109.0 71 881.5 72 784.5 73 769.0 74 675.0 75 575.5 76 428.5 77 284.0 78 210.5 79 164.5 80 125.5 81 87.0 82 63.0 83 46.0 84 24.0 85 11.5 86 5.5 87 1.0 88 0.5 89 1.0 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.034980034980034984 2 0.004950004950004951 3 0.00297000297000297 4 3.3000033000033E-4 5 6.6000066000066E-4 6 0.0 7 0.0 8 0.0 9 0.00132000132000132 10-11 2.75000275000275E-4 12-13 9.9000099000099E-4 14-15 0.0022550022550022553 16-17 0.00352000352000352 18-19 0.0 20-21 0.0 22-23 0.00121000121000121 24-25 7.15000715000715E-4 26-27 0.004015004015004015 28-29 0.004125004125004125 30-31 7.15000715000715E-4 32-33 1.1000011000011E-4 34-35 0.007315007315007315 36-37 0.01045001045001045 38-39 0.007425007425007424 40-41 0.0011000011000011 42-43 2.2000022000022E-4 44-45 0.001155001155001155 46-47 0.0 48-49 3.3000033000033E-4 50-51 0.001155001155001155 52-53 0.0022550022550022553 54-55 0.00319000319000319 56-57 8.25000825000825E-4 58-59 4.4000044000044E-4 60-61 0.0033000033000033 62-63 0.0057750057750057746 64-65 0.009845009845009845 66-67 0.0022000022000022 68-69 0.0033000033000033 70-71 0.0 72-73 4.4000044000044E-4 74-75 0.005885005885005885 76-77 0.00374000374000374 78-79 0.00374000374000374 80-81 0.00484000484000484 82-83 0.004565004565004565 84-85 0.005995005995005995 86-87 0.0034100034100034097 88-89 0.01023001023001023 90-91 0.01397001397001397 92-93 0.001045001045001045 94-95 0.0019250019250019249 96-97 8.8000088000088E-4 98-99 0.002805002805002805 100-101 0.00506000506000506 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 909090.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.368645444276375 #Duplication Level Percentage of deduplicated Percentage of total 1 78.24477300486546 40.975727753596864 2 12.282025205283611 12.863860466263263 3 3.9723123762774817 6.240738552815592 4 1.722856918421633 3.6089473244816435 5 0.9334790309369315 2.4442516200401427 6 0.5639972107883268 1.7721461978000814 7 0.40454673701345484 1.4829895245407574 8 0.2735963823417973 1.1462297553355407 9 0.199933184720852 0.9423207056872182 >10 1.2323214386604147 12.19274089771069 >50 0.10993071190387764 3.9815072407503695 >100 0.05201491715745702 5.03732382304185 >500 0.004422320877020328 1.508098236488999 >1k 0.003158800626443092 3.229260150703537 >5k 6.317601252886183E-4 2.5738577507434552 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8698 0.9567809567809569 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7956 0.8751608751608752 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6685 0.7353507353507354 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4548 0.5002805002805003 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2810 0.3091003091003091 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2706 0.29766029766029767 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2055 0.22605022605022607 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1980 0.21780021780021783 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1811 0.19921019921019922 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1785 0.19635019635019635 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1718 0.18898018898018898 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1700 0.187000187000187 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1636 0.17996017996017996 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1606 0.17666017666017667 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1426 0.15686015686015686 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1273 0.14003014003014003 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1195 0.13145013145013146 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1033 0.11363011363011363 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 920 0.1012001012001012 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.2000022000022E-4 2 0.0 0.0 0.0 0.0 2.2000022000022E-4 3 0.0 0.0 0.0 0.0 2.2000022000022E-4 4 0.0 0.0 0.0 0.0 2.2000022000022E-4 5 0.0 0.0 0.0 0.0 2.2000022000022E-4 6 0.0 0.0 0.0 0.0 2.2000022000022E-4 7 0.0 0.0 0.0 0.0 2.2000022000022E-4 8 0.0 0.0 0.0 0.0 2.2000022000022E-4 9 0.0 0.0 0.0 0.0 2.2000022000022E-4 10-11 0.0 0.0 0.0 0.0 2.2000022000022E-4 12-13 0.0 0.0 0.0 0.0 3.3000033000033E-4 14-15 0.0 0.0 0.0 0.0 3.3000033000033E-4 16-17 0.0 0.0 0.0 0.0 3.3000033000033E-4 18-19 0.0 0.0 0.0 0.0 3.3000033000033E-4 20-21 0.0 0.0 0.0 0.0 3.3000033000033E-4 22-23 0.0 0.0 0.0 0.0 3.3000033000033E-4 24-25 0.0 0.0 0.0 0.0 3.3000033000033E-4 26-27 0.0 0.0 0.0 1.65000165000165E-4 3.3000033000033E-4 28-29 0.0 0.0 0.0 8.8000088000088E-4 3.3000033000033E-4 30-31 0.0 0.0 0.0 0.00275000275000275 3.3000033000033E-4 32-33 0.0 0.0 0.0 0.004620004620004619 3.3000033000033E-4 34-35 0.0 0.0 0.0 0.00693000693000693 3.3000033000033E-4 36-37 0.0 0.0 0.0 0.010065010065010066 3.3000033000033E-4 38-39 0.0 0.0 0.0 0.014025014025014024 3.3000033000033E-4 40-41 0.0 0.0 0.0 0.019855019855019855 3.3000033000033E-4 42-43 0.0 0.0 0.0 0.03113003113003113 3.3000033000033E-4 44-45 0.0 0.0 0.0 0.05021505021505022 3.85000385000385E-4 46-47 0.0 0.0 0.0 0.07161007161007162 4.4000044000044E-4 48-49 0.0 0.0 0.0 0.10884510884510884 4.4000044000044E-4 50-51 0.0 0.0 0.0 0.14921514921514922 4.4000044000044E-4 52-53 0.0 0.0 0.0 0.18903518903518904 5.5000055000055E-4 54-55 0.0 0.0 0.0 0.24772024772024773 6.6000066000066E-4 56-57 0.0 0.0 0.0 0.31674531674531675 6.6000066000066E-4 58-59 0.0 0.0 0.0 0.40007040007040007 6.6000066000066E-4 60-61 0.0 0.0 0.0 0.5015455015455015 6.6000066000066E-4 62-63 0.0 0.0 0.0 0.6261206261206261 6.6000066000066E-4 64-65 0.0 0.0 0.0 0.7362857362857362 6.6000066000066E-4 66-67 0.0 0.0 0.0 0.8826408826408827 8.25000825000825E-4 68-69 0.0 0.0 0.0 1.0796510796510796 9.9000099000099E-4 70-71 0.0 0.0 0.0 1.2355212355212357 9.9000099000099E-4 72-73 0.0 0.0 0.0 1.4316514316514317 0.0011000011000011 74-75 0.0 0.0 0.0 1.6548966548966548 0.00143000143000143 76-77 0.0 0.0 0.0 1.8582868582868581 0.00165000165000165 78-79 0.0 0.0 0.0 2.0706420706420707 0.00165000165000165 80-81 0.0 0.0 0.0 2.3563123563123565 0.00165000165000165 82-83 0.0 0.0 0.0 2.692802692802693 0.00165000165000165 84-85 0.0 0.0 0.0 3.0544280544280547 0.00165000165000165 86-87 0.0 0.0 0.0 3.4503734503734504 0.00176000176000176 88-89 0.0 0.0 0.0 3.8757438757438756 0.00187000187000187 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTAAG 315 0.0 43.73102 2 GGTAAGG 415 0.0 34.336136 3 TAAGGTG 445 0.0 34.154217 5 GTAAGGT 430 0.0 33.13654 4 CGTATAG 90 8.922112E-4 26.401026 1 GCACCGT 235 5.456968E-12 26.275665 6 AAGGTAA 530 0.0 25.105883 1 GTACATG 6780 0.0 24.11138 1 GAGTACT 6930 0.0 23.033924 12-13 TACATGG 7005 0.0 22.851984 2 AGTACTT 7015 0.0 22.822548 12-13 GGTGTGC 815 0.0 22.72926 8 TGCACCG 190 3.2882417E-7 22.497969 5 GTACTTT 7305 0.0 21.915916 14-15 ACATGGG 6955 0.0 21.717422 3 GTGTGCA 790 0.0 21.647186 9 AAGGTGT 745 0.0 21.039526 6 AGGTGTG 755 0.0 20.760855 7 CATGGGG 2185 0.0 20.432938 4 TACTTTT 7835 0.0 20.25151 14-15 >>END_MODULE