FastQCFastQC Report
Thu 26 May 2016
SRR936412_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936412_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957481
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77420.808580013598181No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60610.6330151721026318No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59050.6167224206015576No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT44900.4689388092296349No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36990.3863262038620088No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34760.36303592447265276No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC29190.3048624463566379No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT20850.21775889025474135No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT17680.18465118367884062No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC16110.16825399146301598No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15480.16167422643373602No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC14600.15248344353569418No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14470.151125714243938No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG12050.12585106127432294No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA11270.11770468552378585No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10310.10767837690774021No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT9660.1008897304489593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG75850.028.3813671
TACATGG78050.027.3987182
ACATGGG76850.026.59123
CATGGGA49900.024.760734
GAGTACT59650.024.53817412-13
GTACTTT64550.022.70991514-15
CATGGGG23300.021.8232044
ATGGGAG20550.021.7373525
GGTAAGG3500.021.7252773
GTAAGGT3301.8189894E-1221.6007084
GTATCAA141400.021.509051
AGTACTT62800.021.37769112-13
GTATTAG2901.05501385E-1021.3027651
GTAATAC1353.7823594E-421.1217963
GTAAGCG905.165439E-621.11407394-95
ACTTTTT72350.020.39133516-17
AGAGTAC107100.020.0578810-11
ATGGGAC10450.020.0090775
GTGCTAT2151.073724E-619.8927441
TACTTTT68100.019.67698914-15
Produced by FastQC (version 0.11.5)