##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936412_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 957481 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.627405661313382 31.0 31.0 34.0 30.0 34.0 2 31.677657311215576 33.0 31.0 34.0 30.0 34.0 3 31.626583712888298 33.0 31.0 34.0 30.0 34.0 4 35.32198132391139 37.0 35.0 37.0 33.0 37.0 5 35.365219779818084 37.0 35.0 37.0 33.0 37.0 6 35.44256752875514 37.0 35.0 37.0 33.0 37.0 7 35.32302677546604 37.0 35.0 37.0 33.0 37.0 8 35.31331901102998 37.0 35.0 37.0 33.0 37.0 9 36.84060049233353 39.0 37.0 39.0 33.0 39.0 10-11 36.73076384805547 39.0 37.0 39.0 32.0 39.0 12-13 36.64122107906058 39.0 37.0 39.0 32.0 39.0 14-15 37.63009187649676 40.0 37.0 41.0 32.0 41.0 16-17 37.58296300396562 40.0 37.0 41.0 32.0 41.0 18-19 37.54337266222515 40.0 37.0 41.0 32.0 41.0 20-21 37.55825807509496 40.0 37.0 41.0 32.0 41.0 22-23 37.523738330055636 40.0 37.0 41.0 32.0 41.0 24-25 37.59786147192477 40.0 37.0 41.0 32.0 41.0 26-27 37.50032167740143 40.0 37.0 41.0 32.0 41.0 28-29 37.383933467087076 40.0 37.0 41.0 32.0 41.0 30-31 37.142013784085535 39.0 36.5 41.0 31.0 41.0 32-33 36.915821828318265 39.0 35.5 41.0 31.0 41.0 34-35 36.68349293615226 39.0 35.0 41.0 30.5 41.0 36-37 36.438549694458686 39.0 35.0 40.5 30.0 41.0 38-39 36.226909463477604 39.0 35.0 40.0 30.0 41.0 40-41 35.987333952318636 38.5 35.0 40.0 29.5 41.0 42-43 35.64338822389165 38.0 35.0 40.0 28.5 41.0 44-45 35.37362307972691 38.0 35.0 40.0 27.0 41.0 46-47 35.033104573354464 38.0 35.0 40.0 25.5 41.0 48-49 34.67899206355008 38.0 34.0 40.0 23.5 41.0 50-51 33.63572070881824 37.5 33.0 39.5 22.5 40.5 52-53 33.73319888331987 37.5 33.5 39.5 22.5 40.5 54-55 34.15971074099643 38.0 34.0 40.0 22.5 41.0 56-57 33.98460543864579 38.0 34.0 40.0 21.5 41.0 58-59 33.754237420899216 38.0 33.5 40.0 20.5 41.0 60-61 33.43882855116707 37.0 33.0 40.0 19.5 41.0 62-63 33.13541208650616 37.0 33.0 40.0 18.0 41.0 64-65 32.77313492382616 36.0 32.5 39.5 16.0 41.0 66-67 32.3869152494932 36.0 32.0 39.0 13.0 41.0 68-69 31.94479942682936 35.0 31.5 39.0 9.0 40.0 70-71 31.524603621377345 35.0 31.0 38.0 7.5 40.0 72-73 31.13277391405156 35.0 31.0 37.0 6.5 39.5 74-75 30.74493123101137 35.0 31.0 37.0 2.0 39.0 76-77 30.308828582499288 35.0 30.0 36.0 2.0 39.0 78-79 29.85652352370439 34.0 30.0 36.0 2.0 38.5 80-81 29.40451246552151 34.0 29.0 35.0 2.0 37.0 82-83 29.000612544792013 34.0 29.0 35.0 2.0 37.0 84-85 28.634041302125055 34.0 29.0 35.0 2.0 36.0 86-87 28.343373393310152 34.0 29.0 35.0 2.0 36.0 88-89 28.00227576317441 34.0 27.5 35.0 2.0 36.0 90-91 27.635219915591016 34.0 27.0 35.0 2.0 35.0 92-93 27.214088843538413 34.0 26.0 35.0 2.0 35.0 94-95 26.733334656249056 33.0 25.0 35.0 2.0 35.0 96-97 26.161833498523734 33.0 24.5 35.0 2.0 35.0 98-99 25.47757187871091 33.0 21.0 35.0 2.0 35.0 100-101 23.061028887257294 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4515705122573017 1101 2 0.34008762802750425 1101 3 0.6091041182539421 1101 4 0.5094129761820057 1101 5 0.4094030820379402 1101 6 0.41110543380233366 1101 7 0.49166429876587614 1101 8 0.41803176723740165 1101 9 0.484103640506774 1101 10-11 0.5741437989108178 1101 12-13 0.548570218517753 1101 14-15 0.8357617498553225 1101 16-17 0.6813950197951257 1101 18-19 0.8713905313603547 1101 20-21 0.638284199068373 1101 22-23 0.596516046360648 1101 24-25 0.5157589674759677 1101 26-27 0.3817251758629183 1101 28-29 0.43699066639623396 1101 30-31 0.34426669151040556 1101 32-33 0.4136814203529582 1101 34-35 0.4052199147824709 1101 36-37 0.1965048740290669 1101 38-39 0.35311381740021375 1101 40-41 0.31646482747382976 1101 42-43 0.6127797324351647 1101 44-45 0.4918986432333341 1101 46-47 0.6526386849966812 1101 48-49 0.7022967941448215 1101 50-51 0.6388090944493925 1101 52-53 0.7272153958911787 1101 54-55 0.7202540488206779 1101 56-57 0.7495368235635169 1101 58-59 0.660003073574579 1101 60-61 0.5386926069721838 1101 62-63 0.6497192840117947 1101 64-65 0.44485314391202024 1101 66-67 0.42137032801626617 1101 68-69 0.46649181639442006 1101 70-71 0.34084633918498497 1101 72-73 0.6006948409816459 1101 74-75 0.18940096754692703 1101 76-77 0.40906367884438666 1101 78-79 0.3724851334322885 1101 80-81 0.43665141991674616 1101 82-83 0.503030500136969 1101 84-85 0.5695849573452598 1101 86-87 0.4456409509396231 1101 88-89 0.7664878355360365 1101 90-91 0.5792522257967754 1101 92-93 0.8183426663587596 1101 94-95 0.7793364283095947 1101 96-97 0.9640649357101871 1101 98-99 0.8307943167371832 1101 100-101 1.2600389728608654 1102 1 0.2350497161326892 1102 2 0.30021716996188985 1102 3 0.3228779534511048 1102 4 0.15149903609638926 1102 5 0.24123818533306007 1102 6 0.16331078395339205 1102 7 0.4191687391096437 1102 8 0.2602099689167048 1102 9 0.30747553504521363 1102 10-11 0.3494710503936105 1102 12-13 0.20499506863298933 1102 14-15 0.4715998323184962 1102 16-17 0.3578502050544117 1102 18-19 0.37418689805987526 1102 20-21 0.37325805106350174 1102 22-23 0.1472236605312105 1102 24-25 0.18770958146620131 1102 26-27 0.21674942174556833 1102 28-29 0.11990256849374958 1102 30-31 0.027699824503336856 1102 32-33 0.1489671462843205 1102 34-35 -0.12127084263271826 1102 36-37 -0.07690419835285667 1102 38-39 -0.15958099573480666 1102 40-41 0.16532763011506546 1102 42-43 0.2617245486580444 1102 44-45 0.4960728134016321 1102 46-47 0.39348008999802886 1102 48-49 0.45600222705053994 1102 50-51 0.39308145858969823 1102 52-53 0.09539956084717005 1102 54-55 0.33727223755566627 1102 56-57 0.39222933933013593 1102 58-59 0.316159897376302 1102 60-61 0.44725672701832764 1102 62-63 0.5736893589356526 1102 64-65 0.43503868728905104 1102 66-67 0.29581729215507835 1102 68-69 0.39395240911546736 1102 70-71 -0.02909234825186502 1102 72-73 0.160607368062184 1102 74-75 0.06231450003492611 1102 76-77 0.27036500217799286 1102 78-79 0.14753571259145204 1102 80-81 0.18608448868216598 1102 82-83 0.1226616366426434 1102 84-85 0.20957065437424305 1102 86-87 0.05387911640449161 1102 88-89 0.11054839713144915 1102 90-91 0.36221575692611907 1102 92-93 0.1931607913603024 1102 94-95 0.13562491849389957 1102 96-97 0.21989728710380163 1102 98-99 -0.07976826678945059 1102 100-101 0.29349162113376437 1103 1 0.33443739932940275 1103 2 0.25109114626399176 1103 3 0.33716817895046347 1103 4 0.46344059505403123 1103 5 0.3395123334255743 1103 6 0.28642314007041847 1103 7 0.43841929598345075 1103 8 0.377719798414077 1103 9 0.31910432664287214 1103 10-11 0.292671409784937 1103 12-13 0.26437475307754 1103 14-15 0.5845448054087754 1103 16-17 0.7335337859793043 1103 18-19 0.6091194293079241 1103 20-21 0.5635875512009889 1103 22-23 0.39725118014877125 1103 24-25 0.3324179308969164 1103 26-27 0.3399984196702235 1103 28-29 0.3261192819756431 1103 30-31 0.26110847021089256 1103 32-33 0.4663722524802907 1103 34-35 0.3979348753275431 1103 36-37 0.4379763707570916 1103 38-39 0.24912378868352647 1103 40-41 0.30522440286082286 1103 42-43 0.3869681344620872 1103 44-45 0.2319879756271206 1103 46-47 0.4852120068467016 1103 48-49 0.47357164342638924 1103 50-51 0.3443878860248262 1103 52-53 0.30375366671035664 1103 54-55 0.21508003544649057 1103 56-57 0.14738294554756948 1103 58-59 0.17117698929012448 1103 60-61 0.3907235698105467 1103 62-63 0.4447226557603301 1103 64-65 0.4939021929610661 1103 66-67 0.4617612235375219 1103 68-69 0.3359085417825547 1103 70-71 0.3154026869541191 1103 72-73 0.4919403758352523 1103 74-75 0.31610083814923584 1103 76-77 0.4636028690017433 1103 78-79 0.23568306643678127 1103 80-81 0.541954157473473 1103 82-83 0.622008603555777 1103 84-85 0.44006498320796084 1103 86-87 0.4908834179650974 1103 88-89 0.4100940153070596 1103 90-91 0.49412567788637674 1103 92-93 0.24687717226920824 1103 94-95 0.2979946997012526 1103 96-97 0.5195693467654827 1103 98-99 0.5880412609059107 1103 100-101 0.7714372508697451 1104 1 0.12821788050253247 1104 2 0.15283383708260345 1104 3 0.2746414032666955 1104 4 0.2118793709747706 1104 5 0.28926976617854194 1104 6 0.07465505849814491 1104 7 0.2735535910971336 1104 8 0.3255381079637516 1104 9 0.3487911268552608 1104 10-11 0.37941733026497104 1104 12-13 0.38161831552893233 1104 14-15 0.2864152236996631 1104 16-17 0.30775984989963234 1104 18-19 0.4426083876680664 1104 20-21 0.5016123159176189 1104 22-23 0.26401272502779705 1104 24-25 0.2313706452051818 1104 26-27 -0.12054643592787784 1104 28-29 -0.054637904518401115 1104 30-31 -0.034322210997878244 1104 32-33 0.10894688285979726 1104 34-35 0.26139977812601245 1104 36-37 0.15640604349798792 1104 38-39 0.2032491119259916 1104 40-41 0.1688665943972083 1104 42-43 0.17285152975805573 1104 44-45 0.23426664434344247 1104 46-47 0.5065790577568734 1104 48-49 0.5470222349120277 1104 50-51 0.4720634096124101 1104 52-53 0.24986341683933944 1104 54-55 0.4566564992833477 1104 56-57 0.3454995732416606 1104 58-59 0.2042660974393513 1104 60-61 0.3411169872256039 1104 62-63 0.3045736978511542 1104 64-65 0.5665090050044981 1104 66-67 0.4812873011192522 1104 68-69 0.31723420174098393 1104 70-71 0.10048784081288886 1104 72-73 0.3469199566822354 1104 74-75 0.5994911382004808 1104 76-77 0.44520838873848945 1104 78-79 0.2983853623922563 1104 80-81 0.48618424907560254 1104 82-83 0.42036079094953394 1104 84-85 0.4770893707991597 1104 86-87 0.39526333402485037 1104 88-89 0.2763400522848052 1104 90-91 0.5124394718786327 1104 92-93 0.6980975686974382 1104 94-95 0.5110716673299187 1104 96-97 0.6441319277004425 1104 98-99 0.47806941046686546 1104 100-101 0.9377219288342538 1105 1 0.2420951741149544 1105 2 0.2516055719695345 1105 3 0.17290661643626137 1105 4 0.25971864542746204 1105 5 0.3797429535615464 1105 6 0.21430890195691177 1105 7 0.2950257073769009 1105 8 0.31505731274467763 1105 9 0.32399558744967294 1105 10-11 0.4346592294211078 1105 12-13 0.28753140601214255 1105 14-15 0.6248588206044801 1105 16-17 0.5434626237567031 1105 18-19 0.30868544703921685 1105 20-21 0.40701988571451153 1105 22-23 0.36265933103197767 1105 24-25 0.3082047843685132 1105 26-27 0.41235745507986366 1105 28-29 0.3492731238408098 1105 30-31 0.3428813086273408 1105 32-33 0.4110080058164485 1105 34-35 0.33732798417824483 1105 36-37 0.22769947573319627 1105 38-39 0.1261697201524612 1105 40-41 0.42230263351751063 1105 42-43 0.2318573057072797 1105 44-45 0.31920913510892746 1105 46-47 0.5340723758745298 1105 48-49 0.6764755718490747 1105 50-51 0.6507424490229923 1105 52-53 0.7856152412184514 1105 54-55 0.7017389341913045 1105 56-57 0.39370922918728724 1105 58-59 0.5156618350490874 1105 60-61 0.37916117273668704 1105 62-63 0.4865511346110196 1105 64-65 0.48710221601184145 1105 66-67 0.48514627586323655 1105 68-69 0.3669954558326971 1105 70-71 0.5994717942849661 1105 72-73 0.5294779914580552 1105 74-75 0.5317763920119027 1105 76-77 0.712553754465187 1105 78-79 0.6289858809263471 1105 80-81 0.44171844930183823 1105 82-83 0.2869734019388481 1105 84-85 0.5216452633685087 1105 86-87 0.6186334051981603 1105 88-89 0.5787565412684579 1105 90-91 0.30262836644003244 1105 92-93 0.4320135021088518 1105 94-95 0.46085494410499805 1105 96-97 0.8027553097839863 1105 98-99 1.1438751904202178 1105 100-101 1.3110564634048636 1106 1 0.13018347436376132 1106 2 0.03646348381546005 1106 3 0.13956417386944864 1106 4 0.14836515103016978 1106 5 0.3289499736029171 1106 6 0.22134843285257233 1106 7 0.28879806339540437 1106 8 0.4029259321767924 1106 9 0.5166002668417278 1106 10-11 0.46936461456690637 1106 12-13 0.3821890524192284 1106 14-15 0.501604271548608 1106 16-17 0.36523835231002266 1106 18-19 0.4663247044460306 1106 20-21 0.2442482107700883 1106 22-23 0.20439196020038253 1106 24-25 -0.24170401737537617 1106 26-27 -0.1423948638026289 1106 28-29 0.05306044654175679 1106 30-31 -0.0154664035251173 1106 32-33 0.24174441319831175 1106 34-35 0.22732498660490563 1106 36-37 0.04199053904554262 1106 38-39 0.31712361577971393 1106 40-41 0.09151750232865652 1106 42-43 0.037966211889589374 1106 44-45 -0.16055009513966212 1106 46-47 0.1259662702519151 1106 48-49 0.034505255239658084 1106 50-51 -0.17967963684318278 1106 52-53 0.14120026055438473 1106 54-55 0.3657546550335482 1106 56-57 0.26199606835741207 1106 58-59 0.4716835679854299 1106 60-61 0.34197034382821556 1106 62-63 0.06074160444622123 1106 64-65 -0.12470859368250586 1106 66-67 -0.1562073138632485 1106 68-69 -0.024836103848137725 1106 70-71 -0.19291315132973352 1106 72-73 -0.15988390673672725 1106 74-75 -0.059053522710843964 1106 76-77 -0.19589156418321352 1106 78-79 -0.1811575683166886 1106 80-81 -0.06342138272813358 1106 82-83 0.039455138455330996 1106 84-85 0.1330917037236823 1106 86-87 0.07085388678003213 1106 88-89 0.3603475748169167 1106 90-91 0.2756666241614276 1106 92-93 0.19260060279871993 1106 94-95 0.422539230783169 1106 96-97 0.4126391458728662 1106 98-99 0.41955263669957077 1106 100-101 1.0429129357800555 1107 1 0.08923808095935115 1107 2 0.1316906610098485 1107 3 0.2748196287970366 1107 4 0.29005315337391124 1107 5 0.18382278749212588 1107 6 0.2203294154401121 1107 7 0.2568582363736098 1107 8 0.1661763429681855 1107 9 0.28606668384912837 1107 10-11 0.26956395984822024 1107 12-13 0.19630753866886863 1107 14-15 -0.02885479579570216 1107 16-17 0.1925722206274898 1107 18-19 0.35505118743480324 1107 20-21 0.16016972095389548 1107 22-23 0.19025627803191014 1107 24-25 0.11485335967380905 1107 26-27 0.136643948023071 1107 28-29 0.09755966317212739 1107 30-31 -0.006479943718986192 1107 32-33 0.2230549969679103 1107 34-35 0.21934480649357369 1107 36-37 0.07795866927596506 1107 38-39 0.3311032609068931 1107 40-41 0.29440068472003844 1107 42-43 0.06161378471749401 1107 44-45 0.0240035115450965 1107 46-47 0.031067089287155625 1107 48-49 0.10217756471524098 1107 50-51 0.18139007668555251 1107 52-53 -0.056313973845092846 1107 54-55 0.07877069182007546 1107 56-57 0.23351453167915537 1107 58-59 0.17399355308230469 1107 60-61 0.26635606739426976 1107 62-63 0.13032775599958057 1107 64-65 0.04133074038961837 1107 66-67 0.007516940024942187 1107 68-69 0.12106808303965622 1107 70-71 0.2784429060918967 1107 72-73 0.18320651832091173 1107 74-75 0.13954341768937795 1107 76-77 0.14335843900121503 1107 78-79 0.21648213260170124 1107 80-81 0.17914934344915068 1107 82-83 0.1517413040201845 1107 84-85 0.220704910189351 1107 86-87 0.2047081927654837 1107 88-89 -0.007043231098482039 1107 90-91 0.06744315364288056 1107 92-93 0.22291000196057809 1107 94-95 0.36964267378825255 1107 96-97 0.43835022896099574 1107 98-99 0.6911239615495859 1107 100-101 1.1865052269133294 1108 1 -4.249441875430193E-4 1108 2 -0.08753742273436416 1108 3 0.15942104888232578 1108 4 0.2667159171334461 1108 5 0.07968105109232937 1108 6 0.2527342865546558 1108 7 0.18985570485494918 1108 8 0.41944780269125914 1108 9 0.14284145030131157 1108 10-11 0.1351063028099233 1108 12-13 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2316 76-77 -3.268557080791016 2316 78-79 -3.5829844776525768 2316 80-81 -4.153757570793381 2316 82-83 -3.7418860921757044 2316 84-85 -3.1676644259539586 2316 86-87 -3.908970746255921 2316 88-89 -5.575584279091583 2316 90-91 -4.825067303936073 2316 92-93 -3.942760134599972 2316 94-95 -3.2628911736428563 2316 96-97 -4.129411157293543 2316 98-99 -5.829020847978786 2316 100-101 -7.028245680474564 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2089.0 3 794.0 4 768.0 5 841.0 6 895.0 7 1052.0 8 1256.0 9 1659.0 10 2074.0 11 2756.0 12 3849.0 13 5090.0 14 5901.0 15 7838.0 16 10751.0 17 14729.0 18 10863.0 19 6749.0 20 6286.0 21 6573.0 22 6988.0 23 7730.0 24 8785.0 25 10030.0 26 11690.0 27 13727.0 28 16639.0 29 20132.0 30 24865.0 31 30950.0 32 39310.0 33 51242.0 34 69619.0 35 96778.0 36 132455.0 37 170589.0 38 132567.0 39 20566.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.12742741278037 18.345492171659107 14.684566985095843 20.842513430464685 2 15.511144309272213 20.730347936461506 39.85301107901569 23.905496675250596 3 20.041113404474007 26.25899355722869 29.921805145860663 23.778087892436638 4 12.111487862815025 16.422648557514524 38.00864091523504 33.457222664435406 5 12.770709519844806 38.687055564202986 33.22893738645896 15.313297529493248 6 30.097431225631748 36.18160695704501 17.66862936016749 16.052332457155753 7 27.624603330559953 34.49550336777058 20.361528395051405 17.518364906618057 8 27.14200620735059 32.008819871881954 19.82193054873397 21.02724337203348 9 27.524097877672766 16.634668545097494 20.97683280258605 34.86440077464369 10-11 25.193695567208756 26.44312864060929 26.21886606319899 22.14430972898296 12-13 27.395527721357105 24.600353569547885 26.935333550657404 21.06878515843761 14-15 23.67407195354034 25.033132855830154 27.611096280918556 23.681698909710942 16-17 21.223749626450054 28.76261449436488 27.593324793058553 22.420311086126514 18-19 21.060773076581498 26.695382332812656 31.179048882805603 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21.43354355519512 25.793266808044656 30.189596550530272 22.583593086229957 46-47 22.36057150018799 26.421439612315663 29.601767138739188 21.61622174875715 48-49 22.202215929059356 26.47464454741187 29.018414551166533 22.304724972362248 50-51 21.460664769315127 26.97031254079741 29.496801483067443 22.07222120682002 52-53 22.138309866362814 27.35904282123761 28.321830052935802 22.18081725946377 54-55 21.390189465900626 26.764708646960095 28.83989342869467 23.005208458444606 56-57 22.207385489814964 26.78664401642473 28.25793493921669 22.748035554543616 58-59 22.923154130923056 26.29756683208776 28.282515479831698 22.496763557157486 60-61 22.096836102429126 26.645464349034015 29.51352169973654 21.74417784880032 62-63 21.760023478677276 26.93034572038963 29.219568976186583 22.090061824746503 64-65 21.35352315821026 27.548698195991765 28.848256724168365 22.24952192162961 66-67 21.74343067218114 26.98792664076534 28.203513389313617 23.0651292977399 68-69 21.63030735045681 26.86234114830162 28.737045769655573 22.770305731585996 70-71 22.202070671887387 26.837060702875398 28.37821042396239 22.58265820127482 72-73 21.976736351761485 26.633696513377565 28.97949036409056 22.410076770770388 74-75 21.405207850788845 28.07684293043056 28.161657604622608 22.356291614157993 76-77 21.636359078069898 27.140456034497166 28.222096404873724 23.001088482559208 78-79 21.777282187745637 27.26393389966391 28.376191135263873 22.58259277732658 80-81 22.10354357767022 27.420842411797025 27.96408793036096 22.5115260801718 82-83 21.94728503077442 27.088226969317493 28.44782657284811 22.516661427059976 84-85 21.688766017046206 27.381766169011335 28.029941830255474 22.89952598368699 86-87 21.735895010974218 27.56818349162351 27.896966123795853 22.798955373606418 88-89 21.53903355819654 27.439943350764672 27.42516483527961 23.595858255759182 90-91 21.614070489270045 27.099708440194203 27.825448476217485 23.460772594318264 92-93 22.134705241218025 27.349079774786233 27.941255575638852 22.57495940835689 94-95 21.729936430808184 27.052628940172262 27.929081599612278 23.28835302940728 96-97 21.163256843409258 27.05693971365632 28.51978470431312 23.2600187386213 98-99 21.523031626538273 27.579661781826974 27.35449290516948 23.542813686465266 100-101 21.31819425844718 27.078654384927642 27.70875857357448 23.894392783050698 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 383.0 1 294.5 2 361.5 3 516.0 4 438.0 5 423.5 6 696.5 7 1455.0 8 2419.5 9 2837.0 10 2433.5 11 1955.0 12 1892.0 13 1998.5 14 2147.5 15 2201.0 16 2311.5 17 2404.5 18 2411.5 19 2502.0 20 2673.0 21 2956.0 22 3380.5 23 3954.5 24 4454.5 25 5054.5 26 6039.5 27 7262.5 28 8354.0 29 9689.0 30 11441.5 31 13408.5 32 15958.5 33 17566.5 34 18691.0 35 20004.5 36 21724.5 37 23675.5 38 24111.5 39 23990.5 40 24457.0 41 25669.0 42 26633.0 43 27630.0 44 29031.0 45 30654.0 46 31898.0 47 36706.5 48 48259.0 49 47688.5 50 36419.5 51 30971.5 52 28504.0 53 28421.5 54 28522.0 55 26864.0 56 24643.0 57 22681.0 58 21583.0 59 19681.5 60 16992.0 61 14520.0 62 12375.5 63 9882.0 64 7274.5 65 5602.5 66 4152.0 67 3203.5 68 2402.0 69 1580.5 70 1153.5 71 940.0 72 844.0 73 828.5 74 692.0 75 548.5 76 420.5 77 268.5 78 175.0 79 126.0 80 94.5 81 68.0 82 46.0 83 31.0 84 17.0 85 11.5 86 12.0 87 7.0 88 5.5 89 3.5 90 0.5 91 1.0 92 2.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0732129410400833 2 0.02778123012362647 3 0.013472852202811334 4 0.018277125081333206 5 0.023812482963108405 6 0.030601129421889313 7 0.07989714678411373 8 0.05754683382751198 9 0.06830422744681094 10-11 0.05921788526351959 12-13 0.040209675178933056 14-15 0.03650202980529117 16-17 0.04668499949346253 18-19 0.04135852304118828 20-21 0.016397192215824648 22-23 0.011122936120925637 24-25 0.02000039687471605 26-27 0.02078370223534462 28-29 0.004490950734270444 30-31 8.355257180038037E-4 32-33 0.008041935035786612 34-35 0.009712986471794218 36-37 0.0030287807277637885 38-39 0.005900900383401864 40-41 0.0034465435867656906 42-43 0.0014621700065066564 44-45 5.222035737523773E-5 46-47 1.0444071475047546E-4 48-49 4.6998321637713956E-4 50-51 6.266442885028529E-4 52-53 5.222035737523773E-5 54-55 0.0 56-57 0.002349916081885698 58-59 0.0026632382261371243 60-61 0.004020967517893306 62-63 0.0017232717933828453 64-65 2.0888142950095093E-4 66-67 1.0444071475047546E-4 68-69 0.0024543567966361737 70-71 0.0014621700065066564 72-73 0.015718327569946556 74-75 0.01039185111767231 76-77 0.01932153222883796 78-79 0.015509446140445606 80-81 0.009086342183291365 82-83 0.003133221442514264 84-85 0.002558797511386649 86-87 9.921867901295169E-4 88-89 0.0010444071475047547 90-91 0.0046476118063961585 92-93 0.008303036822662799 94-95 0.01033963076029707 96-97 0.010444071475047546 98-99 0.020835922592719856 100-101 0.01869488794033511 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 957481.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.80919742787128 #Duplication Level Percentage of deduplicated Percentage of total 1 78.79778571503951 42.40045608419656 2 12.019158224230871 12.934825156089232 3 3.873861494089051 6.253481338309983 4 1.6859518970882807 3.6287887393726974 5 0.8839247408639053 2.378164044626292 6 0.5570165569000237 1.7983568328495882 7 0.3618579939225878 1.362990176508378 8 0.2766352427903091 1.1908416315848678 9 0.2028479534252321 0.9823577016328158 >10 1.182463976050252 12.06381291552629 >50 0.10164315921955956 3.7526174391099287 >100 0.050038468983652926 4.919157729428045 >500 0.0036993420154155876 1.3499317796475439 >1k 0.0025311287473896125 2.9194566528256463 >5k 5.841066340129874E-4 2.0647617782921066 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7742 0.808580013598181 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6061 0.6330151721026318 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5905 0.6167224206015576 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4490 0.4689388092296349 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3699 0.3863262038620088 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3476 0.36303592447265276 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2919 0.3048624463566379 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2085 0.21775889025474135 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1768 0.18465118367884062 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1611 0.16825399146301598 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1548 0.16167422643373602 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1460 0.15248344353569418 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1447 0.151125714243938 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1205 0.12585106127432294 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1127 0.11770468552378585 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1031 0.10767837690774021 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 966 0.1008897304489593 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.0888142950095093E-4 14-15 0.0 0.0 0.0 0.0 2.611017868761887E-4 16-17 0.0 0.0 0.0 0.0 4.1776285900190185E-4 18-19 0.0 0.0 0.0 5.222035737523773E-5 5.222035737523774E-4 20-21 0.0 0.0 0.0 1.0444071475047546E-4 5.222035737523774E-4 22-23 0.0 0.0 0.0 2.0888142950095093E-4 5.222035737523774E-4 24-25 0.0 0.0 0.0 3.655425016266641E-4 5.222035737523774E-4 26-27 0.0 0.0 0.0 7.833053606285661E-4 5.222035737523774E-4 28-29 0.0 0.0 0.0 0.0015666107212571321 5.222035737523774E-4 30-31 0.0 0.0 0.0 0.0036032046588914037 5.222035737523774E-4 32-33 0.0 0.0 0.0 0.005953120740777102 5.222035737523774E-4 34-35 0.0 0.0 0.0 0.008773020039039939 5.222035737523774E-4 36-37 0.0 0.0 0.0 0.012271783983180868 5.222035737523774E-4 38-39 0.0 0.0 0.0 0.017389379005954164 5.222035737523774E-4 40-41 0.0 0.0 0.0 0.02349916081885698 5.222035737523774E-4 42-43 0.0 0.0 0.0 0.036032046588914034 6.266442885028528E-4 44-45 0.0 0.0 0.0 0.05363030702436915 6.788646458780905E-4 46-47 0.0 0.0 0.0 0.07545841640721854 7.833053606285661E-4 48-49 0.0 0.0 0.0 0.11295263300263922 9.921867901295169E-4 50-51 0.0 0.0 0.0 0.15060351067018563 0.0010444071475047547 52-53 0.0 0.0 0.0 0.1916487115671225 0.0010444071475047547 54-55 0.0 0.0 0.0 0.24569678145049353 0.0010444071475047547 56-57 0.0 0.0 0.0 0.31133777067116736 0.0010966275048799924 58-59 0.0 0.0 0.0 0.3889894420881459 0.00114884786225523 60-61 0.0 0.0 0.0 0.4793306603473072 0.001201068219630468 62-63 0.0 0.0 0.0 0.5954165147924606 0.0012532885770057056 64-65 0.0 0.0 0.0 0.7083169274377246 0.0012532885770057056 66-67 0.0 0.0 0.0 0.8474319594853579 0.001357729291756181 68-69 0.0 0.0 0.0 1.0237801063415357 0.0014621700065066566 70-71 0.0 0.0 0.0 1.1788223473886166 0.0014621700065066566 72-73 0.0 0.0 0.0 1.3679122614443524 0.0015666107212571321 74-75 0.0 0.0 0.0 1.5736082491454138 0.0016710514360076074 76-77 0.0 0.0 0.0 1.7668757917911688 0.001775492150758083 78-79 0.0 0.0 0.0 1.9765927470101237 0.0019843735802590337 80-81 0.0 0.0 0.0 2.244744282131969 0.0019843735802590337 82-83 0.0 0.0 0.0 2.568771599645319 0.0021932550097599848 84-85 0.0 0.0 0.0 2.927421014098452 0.0021932550097599848 86-87 0.0 0.0 0.0 3.29379904144312 0.0021932550097599848 88-89 0.0 0.0 0.0 3.7015878121863515 0.0021932550097599848 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7585 0.0 28.381367 1 TACATGG 7805 0.0 27.398718 2 ACATGGG 7685 0.0 26.5912 3 CATGGGA 4990 0.0 24.76073 4 GAGTACT 5965 0.0 24.538174 12-13 GTACTTT 6455 0.0 22.709915 14-15 CATGGGG 2330 0.0 21.823204 4 ATGGGAG 2055 0.0 21.737352 5 GGTAAGG 350 0.0 21.725277 3 GTAAGGT 330 1.8189894E-12 21.600708 4 GTATCAA 14140 0.0 21.50905 1 AGTACTT 6280 0.0 21.377691 12-13 GTATTAG 290 1.05501385E-10 21.302765 1 GTAATAC 135 3.7823594E-4 21.121796 3 GTAAGCG 90 5.165439E-6 21.114073 94-95 ACTTTTT 7235 0.0 20.391335 16-17 AGAGTAC 10710 0.0 20.05788 10-11 ATGGGAC 1045 0.0 20.009077 5 GTGCTAT 215 1.073724E-6 19.892744 1 TACTTTT 6810 0.0 19.676989 14-15 >>END_MODULE