FastQCFastQC Report
Thu 26 May 2016
SRR936412_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936412_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957481
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT91600.9566769471143552No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81230.8483719259181122No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72520.7574040633704481No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47410.4951534286320042No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31750.3315992693327596No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT29350.3065334977926455No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC22390.23384276032631457No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20180.21076136236645948No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT19120.19969064660290908No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC18230.19039542299011677No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC17750.18538226868209395No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17590.18371121724608636No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA17420.18193572509532827No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG16760.1750426379217969No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA14180.1480969335161742No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12810.13378855559535907No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12760.1332663520216067No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC10800.1127959719305135No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT700.00839808627.1380836
TAAGGGT2600.025.5724244
GTACATG77650.024.347231
GAGTACT73350.023.50547812-13
TACATGG79550.023.2855762
TAAGGTG4300.023.1935945
AGTACTT74250.023.18857812-13
ACATGGG77800.022.4028723
GTACTTT77600.022.0945614-15
TAGCGCG1302.9445125E-421.919224
GGTGTGC7550.021.3880838
GTAAGGT3800.021.2462624
TACTTTT82700.020.64587414-15
CATGGGA51250.020.2013234
CATGGGG21700.020.1347064
AGAGTAC127050.019.98080310-11
ACTTTTT87600.019.89765216-17
GTACTAG1707.9500685E-519.5594671
GGGATAC1951.0334537E-519.484777
ACTATAC1951.0339199E-519.4837533
Produced by FastQC (version 0.11.5)