##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936412_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 957481 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.790890889740893 31.0 31.0 34.0 30.0 34.0 2 31.939185216207946 31.0 31.0 34.0 30.0 34.0 3 31.98954757326777 31.0 31.0 34.0 30.0 34.0 4 35.735502845487275 37.0 35.0 37.0 35.0 37.0 5 35.58496304365308 37.0 35.0 37.0 33.0 37.0 6 35.69515113093628 37.0 35.0 37.0 33.0 37.0 7 35.63239061662843 37.0 35.0 37.0 33.0 37.0 8 35.60413209243839 37.0 35.0 37.0 33.0 37.0 9 37.19009358932449 39.0 37.0 39.0 33.0 39.0 10-11 37.0736562918742 39.0 37.0 39.0 33.0 39.0 12-13 36.96837274055569 39.0 37.0 39.0 33.0 39.0 14-15 37.96498207275131 40.0 37.0 41.0 33.0 41.0 16-17 37.899254397737394 39.5 37.0 41.0 33.0 41.0 18-19 38.11558401681078 40.0 37.0 41.0 33.0 41.0 20-21 38.154212459568384 40.0 37.0 41.0 33.0 41.0 22-23 38.062537533381864 40.0 37.0 41.0 33.0 41.0 24-25 37.89640682164972 40.0 37.0 41.0 33.0 41.0 26-27 37.651419192652384 39.5 37.0 41.0 32.0 41.0 28-29 37.54631997919541 39.0 36.5 41.0 32.0 41.0 30-31 37.44730652618694 39.0 36.0 41.0 32.0 41.0 32-33 37.20770908247788 39.0 35.5 41.0 32.0 41.0 34-35 36.96355959021641 39.0 35.0 41.0 31.0 41.0 36-37 36.69278084891502 39.0 35.0 40.5 31.0 41.0 38-39 36.48104348806922 39.0 35.0 40.0 30.0 41.0 40-41 36.25368284070389 39.0 35.0 40.0 30.0 41.0 42-43 35.88346661709214 38.0 35.0 40.0 29.5 41.0 44-45 35.45327949066352 38.0 35.0 40.0 27.5 41.0 46-47 34.87127368584859 38.0 34.0 40.0 25.0 41.0 48-49 34.31401093076521 38.0 33.5 40.0 23.0 41.0 50-51 33.94496653197296 38.0 33.0 40.0 22.0 41.0 52-53 33.57314505457549 38.0 33.0 40.0 20.0 41.0 54-55 33.36386205052633 37.0 33.0 40.0 19.5 41.0 56-57 33.01681861049984 37.0 32.5 40.0 18.0 41.0 58-59 32.63583768241877 36.5 32.0 39.5 16.0 41.0 60-61 32.163978188601135 36.0 31.0 39.0 13.0 40.0 62-63 31.844435555379164 35.5 31.0 39.0 9.5 40.0 64-65 31.404004883647822 35.0 30.5 38.5 8.0 40.0 66-67 30.95605709147231 35.0 30.0 38.0 7.0 40.0 68-69 30.569936635818358 35.0 29.0 37.5 3.5 39.5 70-71 30.1748097351279 34.0 29.0 37.0 2.0 39.0 72-73 29.708390558141623 34.0 29.0 36.0 2.0 39.0 74-75 29.203060426264337 34.0 28.5 36.0 2.0 38.5 76-77 27.787497088715078 31.5 26.5 34.5 2.0 36.5 78-79 28.551852726059316 33.5 28.0 35.0 2.0 37.0 80-81 28.30903171968948 34.0 27.0 35.0 2.0 37.0 82-83 27.988403947441256 33.0 27.0 35.0 2.0 36.0 84-85 27.608859079187994 33.0 26.0 35.0 2.0 36.0 86-87 27.2194926061196 33.0 26.0 35.0 2.0 35.5 88-89 26.719199649914724 32.5 25.0 35.0 2.0 35.0 90-91 26.183724794539003 32.0 24.5 34.0 2.0 35.0 92-93 25.48493547130439 32.0 21.5 34.0 2.0 35.0 94-95 24.898619398191713 31.5 19.0 34.0 2.0 35.0 96-97 24.136015753837412 31.0 11.5 34.0 2.0 35.0 98-99 23.249964751258773 31.0 2.0 34.0 2.0 35.0 100-101 20.155403083716543 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2928204598490183 1101 2 0.32358711074733293 1101 3 0.2839927363652528 1101 4 0.2779034122393611 1101 5 0.31939826352526524 1101 6 0.26699403312665737 1101 7 0.3524935749254112 1101 8 0.1953708645471437 1101 9 0.2939642176427384 1101 10-11 0.2663116081444201 1101 12-13 0.28234785589163636 1101 14-15 0.4169271241077155 1101 16-17 0.4899451705429314 1101 18-19 0.3469041812700766 1101 20-21 0.2675340979531242 1101 22-23 0.26558138610528914 1101 24-25 0.331838404618658 1101 26-27 0.33197060794339706 1101 28-29 0.30204037791543925 1101 30-31 0.34702378962578706 1101 32-33 0.43260000404050203 1101 34-35 0.4710279559991051 1101 36-37 0.5017788139590706 1101 38-39 0.5300561926456169 1101 40-41 0.7224780728047975 1101 42-43 0.8026339702226153 1101 44-45 0.6015421009772695 1101 46-47 0.7652685986703247 1101 48-49 0.6190669901399346 1101 50-51 0.5780087003216963 1101 52-53 0.6200436663051434 1101 54-55 0.5878647914290269 1101 56-57 0.47419995190200126 1101 58-59 0.502034639893715 1101 60-61 0.6910521541823691 1101 62-63 0.5207765414652741 1101 64-65 0.5374831936237889 1101 66-67 0.4057865229967845 1101 68-69 0.26018884540713216 1101 70-71 0.16317255431904698 1101 72-73 0.5916694786374528 1101 74-75 0.4960877253738687 1101 76-77 0.4418991530924039 1101 78-79 0.5438329984493855 1101 80-81 0.6148687880786063 1101 82-83 0.6177854294165677 1101 84-85 0.657496768642087 1101 86-87 0.7077649942020834 1101 88-89 0.682160482782173 1101 90-91 0.9110478649296816 1101 92-93 0.6888397875425234 1101 94-95 0.40004407982247514 1101 96-97 0.5695857830083746 1101 98-99 0.6544357048325402 1101 100-101 1.3797017979068222 1102 1 0.26587702204718155 1102 2 0.19375738774078854 1102 3 0.22401165877596085 1102 4 0.24756579789587363 1102 5 0.3092811965483264 1102 6 0.12591708610693786 1102 7 0.24075748079753367 1102 8 0.21539872940259386 1102 9 0.390214072513281 1102 10-11 0.20816348469916335 1102 12-13 0.2513167080226282 1102 14-15 0.2387130933624988 1102 16-17 0.30592843186735763 1102 18-19 0.1812342760550436 1102 20-21 0.13661991824951514 1102 22-23 0.12346506515550715 1102 24-25 0.22573580365178714 1102 26-27 0.1501159893683095 1102 28-29 0.16577925852885755 1102 30-31 0.22078030809984028 1102 32-33 0.365270435427 1102 34-35 0.3732656669308696 1102 36-37 0.36007132577322665 1102 38-39 0.4044531334402137 1102 40-41 0.4288151041601722 1102 42-43 0.43674408580741897 1102 44-45 0.2868730331900764 1102 46-47 0.21207216873141732 1102 48-49 0.41291090742478787 1102 50-51 0.44253498542758507 1102 52-53 0.521019677332518 1102 54-55 0.5448249530847136 1102 56-57 0.3176207087490255 1102 58-59 0.34847964756396266 1102 60-61 0.643592351953675 1102 62-63 0.6466025442171741 1102 64-65 0.4098785830771412 1102 66-67 0.3818370749827764 1102 68-69 0.3875878044275147 1102 70-71 0.3474523787309316 1102 72-73 0.5694961681295858 1102 74-75 0.5223481283767484 1102 76-77 0.36048893943714333 1102 78-79 0.49881569407771664 1102 80-81 0.5257111317896204 1102 82-83 0.6468489924126324 1102 84-85 0.36539687511152863 1102 86-87 0.6716351706547989 1102 88-89 0.5481541193187311 1102 90-91 0.3708371246088902 1102 92-93 0.4569556490186173 1102 94-95 0.2983070963907011 1102 96-97 0.5431345502108478 1102 98-99 0.5338164528853362 1102 100-101 1.2007738537551056 1103 1 0.14891035967314892 1103 2 0.22774315914223564 1103 3 0.1816195473646438 1103 4 0.1945534516816636 1103 5 0.2081309827878428 1103 6 0.12664215896485587 1103 7 0.044480768174167906 1103 8 0.10779001203778193 1103 9 0.1809293998139765 1103 10-11 0.142022474603813 1103 12-13 0.2185668755841661 1103 14-15 0.17392171331425033 1103 16-17 0.19914806561630627 1103 18-19 0.1403233363376728 1103 20-21 0.0356137840311348 1103 22-23 0.15957399098939362 1103 24-25 0.14187173483701798 1103 26-27 0.13448569662332943 1103 28-29 0.2054370462406112 1103 30-31 0.19561186466466296 1103 32-33 0.09491686253061715 1103 34-35 0.13444978826029796 1103 36-37 0.30597009523193464 1103 38-39 0.2898361576400461 1103 40-41 0.39645777159526574 1103 42-43 0.43613142899340573 1103 44-45 0.4825118007022269 1103 46-47 0.5977284765465214 1103 48-49 0.6738612713850145 1103 50-51 0.6099416004211307 1103 52-53 0.5836022627485207 1103 54-55 0.6509724743853269 1103 56-57 0.3286334434254954 1103 58-59 0.5726992910882274 1103 60-61 0.5851238962356113 1103 62-63 0.7503810408342844 1103 64-65 0.7021800603812132 1103 66-67 0.6719858594232022 1103 68-69 0.5762597523106692 1103 70-71 0.414703311316579 1103 72-73 0.6218321055200136 1103 74-75 0.5710718768929226 1103 76-77 0.6343142342795609 1103 78-79 0.49780926234995704 1103 80-81 0.6030958713435339 1103 82-83 0.4400355601191812 1103 84-85 0.8576374054942484 1103 86-87 0.9218792345989151 1103 88-89 0.6892864060140695 1103 90-91 0.6646195611001993 1103 92-93 0.7160442417865021 1103 94-95 0.8205051211834338 1103 96-97 0.9278908921545295 1103 98-99 0.5784109837279239 1103 100-101 1.1355644730293797 1104 1 0.24926153285153063 1104 2 0.23718234755772016 1104 3 0.18529839920563873 1104 4 0.24150622965470347 1104 5 0.12526154024818226 1104 6 0.09928663933864357 1104 7 0.19051275000140322 1104 8 0.18485181153172903 1104 9 0.212420035564314 1104 10-11 0.2901622470994809 1104 12-13 0.23463051737778784 1104 14-15 0.2740365751948275 1104 16-17 0.4386039174046914 1104 18-19 0.20017707533946805 1104 20-21 0.13692412907214901 1104 22-23 0.10219300987086655 1104 24-25 0.1240411742376537 1104 26-27 0.2848327360119285 1104 28-29 0.23516916339973193 1104 30-31 0.38624699604180535 1104 32-33 0.34598645300247455 1104 34-35 0.2831362378742668 1104 36-37 0.29524523187910745 1104 38-39 0.4361247881265484 1104 40-41 0.30406337002875716 1104 42-43 0.26810945403224196 1104 44-45 0.3756811883752107 1104 46-47 0.2642368654870708 1104 48-49 0.5857065465075095 1104 50-51 0.47599752728090294 1104 52-53 0.44671634606185506 1104 54-55 0.5014972888400564 1104 56-57 0.3962173693055391 1104 58-59 0.26909100104915495 1104 60-61 0.4921582800820907 1104 62-63 0.31908683746862465 1104 64-65 0.26278583964037594 1104 66-67 0.2564049204825949 1104 68-69 0.19377632173380022 1104 70-71 0.3060900850461934 1104 72-73 0.45119058997983785 1104 74-75 0.18767017023460397 1104 76-77 0.07044629803099767 1104 78-79 0.18829363198238624 1104 80-81 0.5102962699558198 1104 82-83 0.5583541289702119 1104 84-85 0.6533152298563714 1104 86-87 0.551368762405172 1104 88-89 0.41306635222616706 1104 90-91 0.4410047454208019 1104 92-93 0.49684683365059 1104 94-95 0.5348728480898082 1104 96-97 0.809271020872341 1104 98-99 0.7470565640876892 1104 100-101 1.0204890501559483 1105 1 0.07337036494859106 1105 2 0.1892029287563517 1105 3 0.1400517952035223 1105 4 0.29626262874577236 1105 5 0.26003035212441716 1105 6 0.18370358879658255 1105 7 0.08965887050552368 1105 8 0.1571833129782192 1105 9 0.04990264264961297 1105 10-11 0.06013531936390848 1105 12-13 0.19573045030870873 1105 14-15 0.24624795291387613 1105 16-17 0.30291916210673264 1105 18-19 0.3147701061612196 1105 20-21 0.15949737952011134 1105 22-23 0.19303157359150447 1105 24-25 0.17619489256752985 1105 26-27 0.15037924892023113 1105 28-29 0.11851901374559048 1105 30-31 0.2063567090461973 1105 32-33 0.22525209661539947 1105 34-35 0.3026763642043804 1105 36-37 0.3179436112098202 1105 38-39 0.20653831405825684 1105 40-41 0.17010038815742945 1105 42-43 0.19748170745326377 1105 44-45 0.31906287150884793 1105 46-47 0.5595960854836548 1105 48-49 0.36247161452757126 1105 50-51 0.5898998466698728 1105 52-53 0.6506664146324752 1105 54-55 0.5489161302467096 1105 56-57 0.5440246107642324 1105 58-59 0.5494147344946043 1105 60-61 0.49370487307286837 1105 62-63 0.4166222989939996 1105 64-65 0.4374857805795429 1105 66-67 0.40916726569816575 1105 68-69 0.49189838746150016 1105 70-71 0.5651419084242733 1105 72-73 0.5320088327699111 1105 74-75 0.5731572218978158 1105 76-77 0.29283535876355415 1105 78-79 0.31070471539830535 1105 80-81 0.2668905620767035 1105 82-83 0.6280359011956058 1105 84-85 0.796162362402896 1105 86-87 0.9893598932699597 1105 88-89 0.8161014784061322 1105 90-91 0.9087815555352172 1105 92-93 0.991246064858732 1105 94-95 0.9804868304916781 1105 96-97 1.1403220033843304 1105 98-99 1.1396859723782278 1105 100-101 1.208072260862977 1106 1 -0.07517740080005453 1106 2 0.07868091671733879 1106 3 -0.05554357264533394 1106 4 0.014315964367007439 1106 5 0.08527447428559043 1106 6 -0.016191125411808116 1106 7 0.04291579219895425 1106 8 -0.014606238307294461 1106 9 -0.13238900746684834 1106 10-11 0.02259309647791241 1106 12-13 -0.09477412708110933 1106 14-15 0.045524669183500066 1106 16-17 -0.015585451296338704 1106 18-19 0.15302931045907542 1106 20-21 -0.24256167797080508 1106 22-23 -0.14021199142353424 1106 24-25 -0.1160796300715603 1106 26-27 -0.26619923824792835 1106 28-29 -0.5443522272391803 1106 30-31 -0.34689152709902515 1106 32-33 -0.19178688348940653 1106 34-35 -0.22700808559262242 1106 36-37 -0.3606693914795258 1106 38-39 -0.008717639514308928 1106 40-41 0.12269847924340382 1106 42-43 0.009095609414600858 1106 44-45 0.17294864807629295 1106 46-47 0.37623648366308515 1106 48-49 0.3676228330993965 1106 50-51 0.5112267865923528 1106 52-53 0.25991895965697864 1106 54-55 0.3601661924754822 1106 56-57 0.042288030730617265 1106 58-59 0.3553287454489933 1106 60-61 0.14685571456478996 1106 62-63 0.4038849955177639 1106 64-65 0.18540151381457903 1106 66-67 0.5210069389520804 1106 68-69 0.42730041712582434 1106 70-71 0.4922452675070801 1106 72-73 0.48215431570227807 1106 74-75 0.1562464065896414 1106 76-77 -0.11775619062172993 1106 78-79 -0.13630073001673182 1106 80-81 0.1644212183546081 1106 82-83 0.1522553405116902 1106 84-85 0.4445552182217334 1106 86-87 0.5857149288224512 1106 88-89 -0.018188589245813347 1106 90-91 0.018018741968393925 1106 92-93 0.22621609442137114 1106 94-95 0.1540743961761386 1106 96-97 0.6102567664436336 1106 98-99 0.400013301031958 1106 100-101 1.0115516702880676 1107 1 -0.011049361262180923 1107 2 0.04230836395134219 1107 3 0.025430098492268627 1107 4 0.019474740017585646 1107 5 7.788242161694825E-4 1107 6 -0.04199774138236023 1107 7 -0.04706344532127105 1107 8 -0.10938759538372977 1107 9 -0.061223378924168514 1107 10-11 0.05761545117240274 1107 12-13 0.021737776316037127 1107 14-15 0.007505766410496051 1107 16-17 -0.12188488956299182 1107 18-19 -0.15258674925534166 1107 20-21 -0.1403064650598438 1107 22-23 -0.11550400360079038 1107 24-25 -0.14019849845272603 1107 26-27 -0.3175640846947445 1107 28-29 -0.10817137712390235 1107 30-31 -0.35682057801168554 1107 32-33 -0.16115585695673218 1107 34-35 -0.05259688659125317 1107 36-37 -0.13416729612497846 1107 38-39 -0.2284305313583488 1107 40-41 -0.15977951634933873 1107 42-43 -0.16621678311089738 1107 44-45 -0.22158304973409315 1107 46-47 -0.2490612604871174 1107 48-49 -0.09461938733954867 1107 50-51 -0.05425781987585765 1107 52-53 -0.11002894307780053 1107 54-55 -0.019236281189279225 1107 56-57 -0.21817918875299114 1107 58-59 -0.2478849807155612 1107 60-61 -0.37425386802020455 1107 62-63 -0.2109996644488632 1107 64-65 -0.10044560809811642 1107 66-67 -0.040528975042850135 1107 68-69 -0.08691169134857901 1107 70-71 0.02650972800104512 1107 72-73 -0.057568256807176255 1107 74-75 0.06895164306537183 1107 76-77 0.0018286871598682808 1107 78-79 0.11602708788485572 1107 80-81 0.4323718469609723 1107 82-83 0.28642808943853737 1107 84-85 0.4028169018932566 1107 86-87 0.3246299300325326 1107 88-89 0.298869125352887 1107 90-91 0.20932015458128106 1107 92-93 0.4442921895466476 1107 94-95 0.2495626965824691 1107 96-97 0.5438040934335824 1107 98-99 0.3631407928479753 1107 100-101 0.7995769419198986 1108 1 -0.06777185024273535 1108 2 0.019869870545107915 1108 3 -0.09000070726111531 1108 4 -0.016830889836434437 1108 5 -0.0449424795696487 1108 6 0.09643944866773069 1108 7 -0.0832416040800723 1108 8 -0.010379793150121941 1108 9 -0.049361405771875866 1108 10-11 0.043036481754718636 1108 12-13 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-0.9674681943056207 2316 60-61 -0.46912712642086163 2316 62-63 -0.022680088286819 2316 64-65 -0.2793872904644523 2316 66-67 -0.39752091765123154 2316 68-69 -0.7364936542867078 2316 70-71 -0.39204622137681966 2316 72-73 -0.28530199567105186 2316 74-75 -0.5594776711516154 2316 76-77 -0.6403418325791428 2316 78-79 -0.6094827523510133 2316 80-81 -0.3161153963892822 2316 82-83 -0.4046815049244792 2316 84-85 -0.2587806329919502 2316 86-87 -0.15835189027805185 2316 88-89 -0.5136934072438919 2316 90-91 -0.3265382982292273 2316 92-93 -0.4986667731040946 2316 94-95 -0.8659682716882209 2316 96-97 -0.8618521795172107 2316 98-99 -1.519595287662078 2316 100-101 -2.203395352148288 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 7.0 4 18.0 5 47.0 6 94.0 7 251.0 8 580.0 9 1087.0 10 1723.0 11 2759.0 12 3863.0 13 5405.0 14 7011.0 15 9697.0 16 12584.0 17 16341.0 18 14036.0 19 8482.0 20 7418.0 21 7631.0 22 7968.0 23 8706.0 24 10246.0 25 11841.0 26 14282.0 27 17490.0 28 21323.0 29 26016.0 30 31824.0 31 38310.0 32 47661.0 33 60017.0 34 77524.0 35 103394.0 36 137383.0 37 158994.0 38 81699.0 39 3765.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.80981743769305 19.58421296185067 15.429998631592252 20.175970968864025 2 16.070121049998278 22.393779644924184 38.13685992815758 23.399239376919965 3 20.63174563051832 26.323848757910923 30.208621663938732 22.835783947632024 4 12.78845219905147 17.593038399717592 37.25786725794037 32.360642143290576 5 12.802703837468954 39.476465710102 32.700996601403304 15.01983385102574 6 30.343787500744142 37.170137057549965 16.562939630133652 15.923135811572239 7 27.762430794971387 35.110043959096835 19.71913802989302 17.40838721603875 8 27.60545640070142 32.56064611203773 18.89196756906926 20.941929918191587 9 27.43104040706813 17.017674502157224 20.297321826751656 35.25396326402299 10-11 25.632780180494443 26.99881250907329 25.640769895172856 21.727637415259416 12-13 27.92517150676862 24.948523164445447 26.543665359575424 20.582639969210508 14-15 23.666118873687502 25.716295457287032 27.48333252391568 23.13425314510979 16-17 21.14595485445664 29.117810170645686 27.600338805678646 22.135896169219023 18-19 20.922001000562947 26.898745745257507 31.537159215884515 20.642094038295035 20-21 22.764002627728384 24.726965861463572 31.541461397145216 20.96757011366283 22-23 21.899726365041566 25.25420896520032 31.694343484981406 21.151721184776704 24-25 22.297204853151133 25.463325120811795 31.790186959323474 20.449283066713594 26-27 21.599748085970365 25.330258561760978 32.51132281498262 20.558670537286044 28-29 20.713437490534883 26.751216496094365 31.966091640094707 20.569254373276046 30-31 21.221033612106936 25.42467498896829 31.80604041390439 21.548250985020378 32-33 21.10223069876845 25.972856019293662 31.70554400243155 21.219369279506335 34-35 22.136678268452975 25.49439014901289 31.548485979941876 20.820445602592255 36-37 22.18323079807264 25.008681684283307 31.91099928927685 20.8970882283672 38-39 21.504951839347868 25.81933162571249 31.844569948484157 20.831146586455485 40-41 21.47562582345191 26.018652115948292 31.43543170943264 21.070290351167152 42-43 21.396656096857882 26.379245156896904 30.40365807295257 21.82044067329264 44-45 21.894179888653838 25.85088011095973 30.374442081142433 21.880497919244004 46-47 22.674608359352195 26.76378634089434 29.6235894591889 20.938015840564567 48-49 22.619783681261985 26.92903007490527 28.90495427561631 21.546231968216436 50-51 21.710416034582426 27.313141103134736 29.437309689335713 21.539133172947125 52-53 22.565206425000667 27.74401722858529 28.129143369798904 21.56163297661514 54-55 21.69935616680862 27.164026806103802 28.64767874870035 22.488938278387227 56-57 22.639445976435198 27.047348959639006 28.22877496448567 22.084430099440127 58-59 23.348677547425947 26.459341314831832 28.18197440495249 22.01000673278973 60-61 22.260036613104987 26.955863189205143 29.49475482015967 21.289345377530207 62-63 21.946813361055273 27.040066766628595 29.25411376753577 21.75900610478036 64-65 21.62886592553711 27.93274993536661 28.697591459513383 21.7407926795829 66-67 22.162341972277883 27.072696538207865 28.178949341830762 22.586012147683494 68-69 22.00838454436456 27.18872326825947 28.484630424156848 22.31826176321913 70-71 22.736494372470247 27.205828212689447 28.22088804941719 21.836789365423108 72-73 22.458461316725867 26.808051543581545 28.829867120078625 21.903620019613967 74-75 21.861858317749785 28.488830239165885 27.75324852332728 21.896062919757046 76-77 22.17901210069078 27.363164853895594 27.92966016547846 22.528162879935163 78-79 22.24240408061161 27.628314230490638 27.881864058645995 22.24741763025176 80-81 22.606856779351634 27.71337799506865 27.629186776546998 22.050578449032717 82-83 22.486177803636544 27.402262735066124 28.04200610652257 22.069553354774754 84-85 21.971061004230478 27.865105270938095 27.713990663593187 22.44984306123824 86-87 22.02210221753345 27.894962240304167 27.310966501979383 22.771969040183002 88-89 21.705180947903198 27.872219827867674 27.048231007326724 23.374368216902404 90-91 22.28517424912076 27.31099241650106 27.296416801974345 23.10741653240384 92-93 22.62228924441761 27.735383975717397 27.280215747859575 22.36211103200542 94-95 22.015879234244824 27.578155606048732 27.31651388127138 23.08945127843506 96-97 21.553607645263455 27.4873385250518 27.99949218264289 22.95956164704186 98-99 21.80682347175643 27.947854009198487 26.72837303442875 23.516949484616337 100-101 21.396224936320294 27.692142903794657 26.9429299196656 23.96870224021945 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 794.0 1 572.5 2 470.5 3 594.5 4 527.5 5 477.5 6 728.5 7 1536.0 8 2399.0 9 2811.0 10 2570.5 11 2094.5 12 2035.0 13 2128.5 14 2264.0 15 2401.0 16 2519.0 17 2687.0 18 2832.5 19 2951.5 20 3119.0 21 3346.5 22 3702.5 23 4167.0 24 4708.0 25 5400.0 26 6381.0 27 7570.0 28 8603.5 29 10075.5 30 11869.0 31 13635.0 32 15966.0 33 17626.0 34 18869.0 35 19976.0 36 21596.0 37 23290.5 38 23954.0 39 23824.5 40 24005.5 41 25701.0 42 26635.5 43 27560.5 44 28783.5 45 29891.0 46 30836.0 47 36181.0 48 48682.0 49 47755.5 50 35782.5 51 30443.0 52 28259.0 53 27800.0 54 27607.0 55 26175.5 56 24261.5 57 22545.0 58 21535.5 59 19965.5 60 17172.5 61 14390.0 62 12337.5 63 9915.5 64 7262.0 65 5479.0 66 3931.5 67 3105.0 68 2456.0 69 1636.5 70 1184.0 71 972.0 72 916.0 73 857.0 74 700.0 75 598.0 76 440.0 77 290.5 78 220.5 79 160.5 80 115.0 81 86.0 82 66.5 83 45.5 84 27.5 85 13.0 86 6.5 87 6.0 88 4.0 89 1.5 90 1.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01712827721907798 2 0.011279597193051352 3 0.010652952904548497 4 0.0 5 0.002819899298262838 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.001201068219630468 14-15 2.611017868761887E-4 16-17 0.0 18-19 0.0020888142950095095 20-21 0.0 22-23 1.0444071475047546E-4 24-25 0.0 26-27 0.001201068219630468 28-29 0.0018799328655085582 30-31 0.001409949649131419 32-33 0.007101968603032332 34-35 0.001409949649131419 36-37 5.74423931127615E-4 38-39 8.877460753790415E-4 40-41 2.611017868761887E-4 42-43 0.003864306445767592 44-45 0.0017232717933828453 46-47 2.611017868761887E-4 48-49 5.222035737523774E-4 50-51 5.222035737523773E-5 52-53 2.611017868761887E-4 54-55 0.0013055089343809432 56-57 0.012637326484807532 58-59 0.023551381176232216 60-61 0.01812046400920749 62-63 0.012167343268430391 64-65 0.016553853287950362 66-67 0.0015143903638818942 68-69 5.222035737523774E-4 70-71 5.222035737523773E-5 72-73 0.0 74-75 7.83305360628566E-4 76-77 0.001566610721257132 78-79 0.007885273963660898 80-81 0.0142561575634399 82-83 0.015039462924068466 84-85 0.02720680619249886 86-87 0.011592919337302776 88-89 0.038486403385550205 90-91 0.04208960804444161 92-93 0.026371280474495058 94-95 0.02673682297612172 96-97 0.046371677349211105 98-99 0.04966155986385109 100-101 0.056763528466883414 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 957481.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.76394240138512 #Duplication Level Percentage of deduplicated Percentage of total 1 77.88487292602679 40.3162807608205 2 12.497260498816837 12.938149452717203 3 3.9573366935270746 6.1454204599987 4 1.754697720307494 3.6332028690335556 5 0.9676334792862552 2.5044261843712796 6 0.5984719037234232 1.8587559091911947 7 0.420241209101825 1.5227339219844716 8 0.29422767912551756 1.218430770811721 9 0.20524129291400028 0.956168861830751 >10 1.242629889718002 12.21007949660508 >50 0.11382074278068746 4.061015184899441 >100 0.0552922994983181 5.226134901920779 >500 0.004641324365777761 1.623798576840541 >1k 0.0030269506733333223 3.2225272496320803 >5k 6.053901346666645E-4 2.5628753993427256 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9160 0.9566769471143552 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8123 0.8483719259181122 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7252 0.7574040633704481 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4741 0.4951534286320042 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3175 0.3315992693327596 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2935 0.3065334977926455 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2239 0.23384276032631457 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2018 0.21076136236645948 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1912 0.19969064660290908 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1823 0.19039542299011677 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1775 0.18538226868209395 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1759 0.18371121724608636 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1742 0.18193572509532827 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1676 0.1750426379217969 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1418 0.1480969335161742 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1281 0.13378855559535907 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1276 0.1332663520216067 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1080 0.1127959719305135 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.0888142950095093E-4 2 0.0 0.0 0.0 0.0 2.0888142950095093E-4 3 0.0 0.0 0.0 0.0 2.0888142950095093E-4 4 0.0 0.0 0.0 0.0 2.0888142950095093E-4 5 0.0 0.0 0.0 0.0 2.0888142950095093E-4 6 0.0 0.0 0.0 0.0 2.0888142950095093E-4 7 0.0 0.0 0.0 0.0 2.0888142950095093E-4 8 0.0 0.0 0.0 0.0 2.0888142950095093E-4 9 0.0 0.0 0.0 0.0 2.0888142950095093E-4 10-11 0.0 0.0 0.0 0.0 2.0888142950095093E-4 12-13 0.0 0.0 0.0 0.0 3.655425016266641E-4 14-15 0.0 0.0 0.0 0.0 4.699832163771396E-4 16-17 0.0 0.0 0.0 0.0 5.222035737523774E-4 18-19 0.0 0.0 0.0 5.222035737523773E-5 5.222035737523774E-4 20-21 0.0 0.0 0.0 1.0444071475047546E-4 5.222035737523774E-4 22-23 0.0 0.0 0.0 2.0888142950095093E-4 5.222035737523774E-4 24-25 0.0 0.0 0.0 3.655425016266641E-4 5.222035737523774E-4 26-27 0.0 0.0 0.0 7.833053606285661E-4 7.833053606285661E-4 28-29 0.0 5.222035737523773E-5 0.0 0.0015666107212571321 8.355257180038037E-4 30-31 0.0 1.0444071475047546E-4 0.0 0.0036032046588914037 8.355257180038037E-4 32-33 0.0 1.0444071475047546E-4 0.0 0.006057561455527577 8.355257180038037E-4 34-35 0.0 1.0444071475047546E-4 0.0 0.00898190146854089 8.355257180038037E-4 36-37 0.0 1.0444071475047546E-4 0.0 0.012480665412681818 8.355257180038037E-4 38-39 0.0 1.0444071475047546E-4 0.0 0.017546040078079878 8.877460753790415E-4 40-41 0.0 1.0444071475047546E-4 0.0 0.02344694046148174 9.399664327542792E-4 42-43 0.0 1.0444071475047546E-4 0.0 0.03577094480203785 9.399664327542792E-4 44-45 0.0 1.0444071475047546E-4 0.0 0.05321254416536725 9.399664327542792E-4 46-47 0.0 1.0444071475047546E-4 0.0 0.07519731462034233 9.399664327542792E-4 48-49 0.0 1.0444071475047546E-4 0.0 0.11222154799938588 9.921867901295169E-4 50-51 0.0 1.0444071475047546E-4 0.0 0.1496113238800561 0.00114884786225523 52-53 0.0 1.0444071475047546E-4 0.0 0.1907087451343682 0.00114884786225523 54-55 0.0 1.0444071475047546E-4 0.0 0.2443912725161126 0.0012532885770057056 56-57 0.0 1.0444071475047546E-4 0.0 0.30966671923515976 0.0013055089343809432 58-59 0.0 1.0444071475047546E-4 0.0 0.3874228313668887 0.001357729291756181 60-61 0.0 1.0444071475047546E-4 0.0 0.47708518498017194 0.001357729291756181 62-63 0.0 1.0444071475047546E-4 0.0 0.5933799208548264 0.001357729291756181 64-65 0.0 1.0444071475047546E-4 0.0 0.7062803335000903 0.001357729291756181 66-67 0.0 1.0444071475047546E-4 0.0 0.8449253823313465 0.001357729291756181 68-69 0.0 1.0444071475047546E-4 0.0 1.0189236131056387 0.001357729291756181 70-71 0.0 1.0444071475047546E-4 0.0 1.1727125655757136 0.001357729291756181 72-73 0.0 1.0444071475047546E-4 0.0 1.3601836485528171 0.001357729291756181 74-75 0.0 1.0444071475047546E-4 0.0 1.5633730590998671 0.001409949649131419 76-77 0.0 1.0444071475047546E-4 0.0 1.7544995670932373 0.0014621700065066566 78-79 0.0 1.0444071475047546E-4 0.0 1.9627543523056854 0.0014621700065066566 80-81 0.0 1.0444071475047546E-4 0.0 2.226728258837512 0.0014621700065066566 82-83 0.0 1.0444071475047546E-4 0.0 2.5463690663313425 0.001566610721257132 84-85 0.0 1.0444071475047546E-4 0.0 2.8988042582568214 0.0016710514360076074 86-87 0.0 1.0444071475047546E-4 0.0 3.2578714355689566 0.0016710514360076074 88-89 0.0 1.0444071475047546E-4 0.0 3.657096067702649 0.0016710514360076074 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGT 70 0.008398086 27.138083 6 TAAGGGT 260 0.0 25.572424 4 GTACATG 7765 0.0 24.34723 1 GAGTACT 7335 0.0 23.505478 12-13 TACATGG 7955 0.0 23.285576 2 TAAGGTG 430 0.0 23.193594 5 AGTACTT 7425 0.0 23.188578 12-13 ACATGGG 7780 0.0 22.402872 3 GTACTTT 7760 0.0 22.09456 14-15 TAGCGCG 130 2.9445125E-4 21.91922 4 GGTGTGC 755 0.0 21.388083 8 GTAAGGT 380 0.0 21.246262 4 TACTTTT 8270 0.0 20.645874 14-15 CATGGGA 5125 0.0 20.201323 4 CATGGGG 2170 0.0 20.134706 4 AGAGTAC 12705 0.0 19.980803 10-11 ACTTTTT 8760 0.0 19.897652 16-17 GTACTAG 170 7.9500685E-5 19.559467 1 GGGATAC 195 1.0334537E-5 19.48477 7 ACTATAC 195 1.0339199E-5 19.483753 3 >>END_MODULE