FastQCFastQC Report
Thu 26 May 2016
SRR936411_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936411_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076388
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108141.0046563135226332No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84040.7807593544335314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50940.4732494230704913No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28520.26496021880585807No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20260.18822209091888797No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18320.17019885022872794No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18180.16889820399335556No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16850.156542064757318No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13360.12411881217553522No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13220.12281816594016284No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13010.12086719658710428No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG11850.11009041349401888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA145000.028.8659361
GGTATCA101550.026.6201251
GTACATG112100.025.9372271
TCGACCG751.0581825E-625.34059718-19
TACATGG114200.025.2522662
ACATGGG113400.024.5098573
TAAGGTG4900.024.2427715
ATGGGAG24450.024.098015
ATCAACG174600.023.9461883
TCAACGC175050.023.8597054
TATCAAC177200.023.674172
GAGTACT83700.023.64162812-13
CAACGCA176400.023.6232345
CATGGGC13300.023.5792854
AACGCAG180550.023.2129126
CATGGGG34000.022.7796484
AGTACTT85450.022.62925112-13
ATACCGT1050.002191289322.6276386
GTACTTT89000.022.05050714-15
CATGGGA73250.021.730794
Produced by FastQC (version 0.11.5)