##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936411_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1076388 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.681053672095935 31.0 31.0 34.0 30.0 34.0 2 31.729800963964667 33.0 31.0 34.0 30.0 34.0 3 31.713138756656523 33.0 31.0 34.0 30.0 34.0 4 35.38046875290323 37.0 35.0 37.0 33.0 37.0 5 35.409788106147595 37.0 35.0 37.0 33.0 37.0 6 35.4469958788095 37.0 35.0 37.0 33.0 37.0 7 35.38001445575387 37.0 35.0 37.0 33.0 37.0 8 35.3449146590263 37.0 35.0 37.0 33.0 37.0 9 36.85465092513109 39.0 37.0 39.0 33.0 39.0 10-11 36.74426972430016 39.0 37.0 39.0 32.0 39.0 12-13 36.64979589144435 39.0 37.0 39.0 32.0 39.0 14-15 37.69498545134283 40.0 37.0 41.0 32.0 41.0 16-17 37.64233761431751 40.0 37.0 41.0 32.0 41.0 18-19 37.71094716774992 40.0 37.0 41.0 32.0 41.0 20-21 37.62788975722509 40.0 37.0 41.0 32.0 41.0 22-23 37.69732196940137 40.0 37.0 41.0 32.0 41.0 24-25 37.719259226226974 40.0 37.0 41.0 32.0 41.0 26-27 37.60802192146326 40.0 37.0 41.0 32.0 41.0 28-29 37.463909854067495 40.0 37.0 41.0 32.0 41.0 30-31 37.16508405890812 39.0 36.0 41.0 31.5 41.0 32-33 36.867056767633976 39.0 35.0 41.0 31.0 41.0 34-35 36.63617580277744 39.0 35.0 41.0 30.5 41.0 36-37 36.39844925807422 39.0 35.0 41.0 30.0 41.0 38-39 36.14990365927528 38.5 35.0 40.0 30.0 41.0 40-41 35.86945971155383 38.0 35.0 40.0 29.5 41.0 42-43 35.52819382973426 38.0 35.0 40.0 28.5 41.0 44-45 35.08001761446616 38.0 34.5 40.0 26.5 41.0 46-47 34.5605529790373 38.0 34.0 40.0 23.5 41.0 48-49 34.202296941251674 38.0 34.0 40.0 21.5 41.0 50-51 33.16565541421866 37.0 32.5 39.5 19.0 40.5 52-53 33.21837989646856 37.5 33.0 39.5 18.0 40.5 54-55 33.62209305566394 38.0 33.5 40.0 18.0 41.0 56-57 33.50193517579163 38.0 33.5 40.0 16.0 41.0 58-59 33.261946900188406 37.0 33.0 40.0 13.5 41.0 60-61 32.948005273191455 37.0 33.0 40.0 9.5 41.0 62-63 32.58352424962003 36.0 32.5 40.0 7.5 41.0 64-65 32.215521261849815 36.0 32.0 39.0 6.5 41.0 66-67 31.776086318316445 35.0 31.5 39.0 2.0 41.0 68-69 31.39638030152696 35.0 31.0 38.5 2.0 40.0 70-71 30.998612489176764 35.0 31.0 37.5 2.0 40.0 72-73 30.636695596755075 35.0 31.0 37.0 2.0 39.5 74-75 30.228922098722762 35.0 30.0 36.5 2.0 39.0 76-77 29.795245301879994 34.5 30.0 36.0 2.0 39.0 78-79 29.331611835137515 34.0 29.0 36.0 2.0 37.5 80-81 28.9202936115973 34.0 29.0 35.0 2.0 37.0 82-83 28.529362088763534 34.0 29.0 35.0 2.0 37.0 84-85 28.193913347231668 34.0 28.0 35.0 2.0 36.0 86-87 27.855095467433678 34.0 27.0 35.0 2.0 36.0 88-89 27.456772093334372 34.0 26.5 35.0 2.0 35.5 90-91 27.056297543265067 33.5 25.5 35.0 2.0 35.0 92-93 26.631790302381667 33.0 25.0 35.0 2.0 35.0 94-95 26.168970204052812 33.0 24.0 35.0 2.0 35.0 96-97 25.61483777225313 33.0 21.5 35.0 2.0 35.0 98-99 24.945253477370613 33.0 10.0 35.0 2.0 35.0 100-101 22.27185875353497 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4835831656770324 1101 2 0.44083753317302765 1101 3 0.5392192752429317 1101 4 0.54406944379609 1101 5 0.33744017065698273 1101 6 0.4118814257145047 1101 7 0.5066855292901522 1101 8 0.5498936855115275 1101 9 0.7045826326575337 1101 10-11 0.7671344500874113 1101 12-13 0.7367656260136286 1101 14-15 0.804505407509815 1101 16-17 0.943235768738802 1101 18-19 0.7534694771789319 1101 20-21 0.8163382355142588 1101 22-23 0.7643298735936384 1101 24-25 0.7649528395551712 1101 26-27 0.7211994237926262 1101 28-29 0.6808589161783942 1101 30-31 0.6348449078458174 1101 32-33 0.6268518019290568 1101 34-35 0.47094674396225145 1101 36-37 0.480253692694113 1101 38-39 0.3569087351223601 1101 40-41 0.4897935514360512 1101 42-43 0.6574217860876033 1101 44-45 0.5196066615151054 1101 46-47 0.5506607850222665 1101 48-49 0.5333254293398397 1101 50-51 0.36489510378223855 1101 52-53 0.41040338168904356 1101 54-55 0.4922759883100625 1101 56-57 0.3445469905710894 1101 58-59 0.43157997613575105 1101 60-61 0.5440620895849051 1101 62-63 0.6592818682220312 1101 64-65 0.3573333400724721 1101 66-67 0.417696038129975 1101 68-69 0.2987462331077211 1101 70-71 0.16603671236542894 1101 72-73 0.29752699035470087 1101 74-75 0.33267916309009493 1101 76-77 0.30273028656053214 1101 78-79 0.184446558830075 1101 80-81 0.3078478161785192 1101 82-83 0.21941661488369846 1101 84-85 0.1278219307814652 1101 86-87 0.34600610593256675 1101 88-89 0.21035488045373896 1101 90-91 0.140080051551827 1101 92-93 0.15542789351524888 1101 94-95 0.29774865333271805 1101 96-97 0.3242876667071535 1101 98-99 -0.11108232403318752 1101 100-101 -0.10413465669737221 1102 1 0.3896508484894383 1102 2 0.4331281703144718 1102 3 0.32406813920311706 1102 4 0.3370168919636214 1102 5 0.23474481281660786 1102 6 0.365705872985437 1102 7 0.5415854785523635 1102 8 0.44123281546280424 1102 9 0.6595684886317343 1102 10-11 0.623181281756132 1102 12-13 0.4797443508966879 1102 14-15 0.47414197207537967 1102 16-17 0.38793054274715644 1102 18-19 0.3910084861935772 1102 20-21 0.444033419595101 1102 22-23 0.2338267354965069 1102 24-25 0.36697748560700916 1102 26-27 0.24593409298923063 1102 28-29 0.1204962452858922 1102 30-31 0.5452708272074887 1102 32-33 0.3316586855866248 1102 34-35 0.34854601278214403 1102 36-37 0.21471959064862034 1102 38-39 0.2664349276340232 1102 40-41 0.40290440306626607 1102 42-43 0.2484943689187702 1102 44-45 0.4573468840663182 1102 46-47 0.4884069153975119 1102 48-49 0.6448182322200822 1102 50-51 0.8247034817717704 1102 52-53 0.9063346285179321 1102 54-55 0.818673536215563 1102 56-57 0.5877686312780881 1102 58-59 0.5244252536992207 1102 60-61 0.708612365167518 1102 62-63 0.5128592294402878 1102 64-65 0.7752177135303526 1102 66-67 0.7808836914727664 1102 68-69 0.6520671252586538 1102 70-71 0.4965780775593096 1102 72-73 0.4151451642115376 1102 74-75 0.3687825708620096 1102 76-77 0.5748818465736001 1102 78-79 0.45633782706613957 1102 80-81 0.7296049420568735 1102 82-83 0.6927963072598224 1102 84-85 0.14832312277184556 1102 86-87 0.4504199455316673 1102 88-89 0.40362830150088413 1102 90-91 0.3435357810985913 1102 92-93 0.021669544369796512 1102 94-95 0.11281603727872636 1102 96-97 0.09484376670923567 1102 98-99 -0.005557118475660872 1102 100-101 0.1583089583349171 1103 1 0.18036024041951748 1103 2 0.09351815666701668 1103 3 0.2144253165235277 1103 4 0.27443917729191725 1103 5 0.3249455268955401 1103 6 0.26249941043322167 1103 7 0.2607284459776835 1103 8 0.3305216314427355 1103 9 -0.002033226260529375 1103 10-11 0.1960731635884656 1103 12-13 -0.2527848394921932 1103 14-15 0.1810246315369355 1103 16-17 0.31355984203996456 1103 18-19 0.2539863194189209 1103 20-21 0.3692850014591542 1103 22-23 0.16809287464541 1103 24-25 0.20004774931994262 1103 26-27 0.15355013081611446 1103 28-29 0.2878605633956539 1103 30-31 0.1940515167485799 1103 32-33 -0.005025051885972687 1103 34-35 0.18942841032284008 1103 36-37 0.3177509797940701 1103 38-39 0.3610079966570581 1103 40-41 0.2851337630144357 1103 42-43 0.4359573209045422 1103 44-45 0.4244896033560934 1103 46-47 0.21929117395474407 1103 48-49 0.54706661589276 1103 50-51 0.6448476071629301 1103 52-53 0.5517482566730436 1103 54-55 0.43735228340680266 1103 56-57 0.46364399831190184 1103 58-59 0.20527263655857553 1103 60-61 0.2319457214763716 1103 62-63 0.31770131874150564 1103 64-65 0.4819563165272811 1103 66-67 0.43078960674826305 1103 68-69 0.30071065098893257 1103 70-71 0.27891978776457194 1103 72-73 0.30634600235824294 1103 74-75 0.4738786544561151 1103 76-77 0.4979074458898509 1103 78-79 0.4872848087730368 1103 80-81 0.49462472397330615 1103 82-83 0.003562287877681314 1103 84-85 0.15062768157307715 1103 86-87 0.3505505613213593 1103 88-89 0.08576598915338352 1103 90-91 -0.030363806720714592 1103 92-93 -0.07503783490536975 1103 94-95 0.012907364941703747 1103 96-97 -0.028800448286421698 1103 98-99 -0.13440769952637766 1103 100-101 -0.002130310492368892 1104 1 0.27172236666536165 1104 2 0.22308626297931156 1104 3 0.18452498429761377 1104 4 0.2146385128400894 1104 5 0.19953024450351364 1104 6 0.37545622106445364 1104 7 0.29561216690791525 1104 8 0.37204987064539097 1104 9 0.41075747141388774 1104 10-11 0.5332824659140556 1104 12-13 0.5499560242951844 1104 14-15 0.44763592721799483 1104 16-17 0.3683771177874746 1104 18-19 0.2664447911797154 1104 20-21 0.026677028037227046 1104 22-23 0.3113652998945753 1104 24-25 0.38775539051596297 1104 26-27 0.013599964703153944 1104 28-29 0.22889046372787902 1104 30-31 0.204433576549242 1104 32-33 0.25618757269874237 1104 34-35 0.22555797842915837 1104 36-37 0.08602340504324246 1104 38-39 0.004280421906230458 1104 40-41 -0.13347088814835217 1104 42-43 -0.22807922394594726 1104 44-45 -0.15026122720869495 1104 46-47 -0.42107427455024293 1104 48-49 -0.19047491234643843 1104 50-51 -0.4544048845314208 1104 52-53 -0.33446436791167855 1104 54-55 -0.5501892448323957 1104 56-57 -0.38062510467813127 1104 58-59 -0.6896609182587028 1104 60-61 -0.27926690310834346 1104 62-63 -0.13329535899935507 1104 64-65 -0.27717989609730154 1104 66-67 -0.3532635493979157 1104 68-69 -0.21216310316389553 1104 70-71 -0.3332064580826035 1104 72-73 -0.45445133983445274 1104 74-75 -0.4704735050123219 1104 76-77 -0.5432055109207816 1104 78-79 -0.5401238291007218 1104 80-81 -0.49665434579413414 1104 82-83 -0.5109725958432456 1104 84-85 -0.6276115210847308 1104 86-87 -0.6212102360208327 1104 88-89 -0.664649293238643 1104 90-91 -0.4788687901094164 1104 92-93 -0.5438916555033764 1104 94-95 -0.6635876516695909 1104 96-97 -0.6828702157282827 1104 98-99 -0.5334940782639173 1104 100-101 -0.3497216726186174 1105 1 0.2548237865898173 1105 2 0.33300241006891795 1105 3 0.36056774131759184 1105 4 0.4057565895706219 1105 5 0.2623658836970719 1105 6 0.4713210324238517 1105 7 0.5342061231269142 1105 8 0.5525668687794649 1105 9 0.5020058216619177 1105 10-11 0.4743692542451896 1105 12-13 0.3442902640439627 1105 14-15 0.4923001173604433 1105 16-17 0.5282850161172377 1105 18-19 0.4354746801343481 1105 20-21 0.5365911840468769 1105 22-23 0.1282513646103638 1105 24-25 0.4021779204390441 1105 26-27 0.17100194668103086 1105 28-29 0.11514148771185262 1105 30-31 0.10766993480710596 1105 32-33 0.17276558890043958 1105 34-35 0.1945469080245701 1105 36-37 0.35157089514472517 1105 38-39 0.3268296869132783 1105 40-41 0.30320708026742693 1105 42-43 0.08590839722548793 1105 44-45 0.35141696875908934 1105 46-47 0.40444454399979435 1105 48-49 0.364305386201778 1105 50-51 0.22434431903658947 1105 52-53 0.31591194620109775 1105 54-55 0.29504410973912343 1105 56-57 0.05908357187946223 1105 58-59 0.16773793487924138 1105 60-61 0.24196369808402807 1105 62-63 0.06817206693246902 1105 64-65 0.21938833536858482 1105 66-67 0.3134253984016624 1105 68-69 0.3427963925808868 1105 70-71 0.3812172096004609 1105 72-73 0.16447302188220192 1105 74-75 0.35236162218923894 1105 76-77 0.25762533830326717 1105 78-79 0.5176615779898022 1105 80-81 0.6830306653559113 1105 82-83 0.6940830406196845 1105 84-85 0.5975213123927183 1105 86-87 0.46113798679808227 1105 88-89 0.3748352446032541 1105 90-91 0.16889636485349513 1105 92-93 0.18380967112475943 1105 94-95 0.2979773940683579 1105 96-97 0.6662457447686911 1105 98-99 0.6998917816550723 1105 100-101 0.6097228729600523 1106 1 0.3203080100141342 1106 2 0.24361757794207506 1106 3 0.39091040470171023 1106 4 0.37390769799646506 1106 5 0.2992021269070477 1106 6 0.3418679894758796 1106 7 0.4222243390299738 1106 8 0.4827580623559058 1106 9 0.4976127737449474 1106 10-11 0.6597891912292297 1106 12-13 0.6724246162105629 1106 14-15 0.6252600463263249 1106 16-17 0.52176285042367 1106 18-19 0.5050212729812458 1106 20-21 0.3236515263432338 1106 22-23 0.42108852256809826 1106 24-25 0.34790676830859013 1106 26-27 0.33896156508440356 1106 28-29 0.30223316858911176 1106 30-31 0.5509958052291353 1106 32-33 0.33779039155208324 1106 34-35 0.506106397685663 1106 36-37 0.36402021698912534 1106 38-39 0.6007895848229126 1106 40-41 0.46050275836655885 1106 42-43 0.5657712415263845 1106 44-45 0.5171006580158846 1106 46-47 0.6814183099373068 1106 48-49 0.7310041913830929 1106 50-51 0.38848895929226757 1106 52-53 0.5933716718896989 1106 54-55 0.6322648858066131 1106 56-57 0.6331672007177289 1106 58-59 0.7450171813981115 1106 60-61 0.8262983602484795 1106 62-63 0.9553662334391575 1106 64-65 0.9790048330139527 1106 66-67 1.0748706857566788 1106 68-69 1.2775952045381018 1106 70-71 1.0151144726977321 1106 72-73 0.7930953101067999 1106 74-75 1.2201548073183766 1106 76-77 1.159315010123386 1106 78-79 1.0160399475930717 1106 80-81 1.216273912032893 1106 82-83 1.0243726260469614 1106 84-85 1.2553325948465002 1106 86-87 1.3848320714291908 1106 88-89 1.2079562425817763 1106 90-91 1.0948967290586218 1106 92-93 1.201789476091502 1106 94-95 1.3216539036845028 1106 96-97 1.1590985720168838 1106 98-99 1.0884979488619315 1106 100-101 1.0031985192124075 1107 1 0.15943778800808062 1107 2 0.11323127399435862 1107 3 0.06615482948634366 1107 4 0.014192587589555217 1107 5 0.07186895660260006 1107 6 0.1686039592256634 1107 7 0.2620640378284591 1107 8 0.38499221293363206 1107 9 0.33238819016294485 1107 10-11 0.3568362458369023 1107 12-13 0.16263814115025355 1107 14-15 -0.16557919793316955 1107 16-17 -0.05947464721387519 1107 18-19 8.657298748957487E-4 1107 20-21 -0.019429895747364867 1107 22-23 -0.021125752936711706 1107 24-25 0.12071686923520986 1107 26-27 0.20424427004525114 1107 28-29 0.14425987055311396 1107 30-31 0.28636909960714263 1107 32-33 0.046454609172620565 1107 34-35 0.06705966958735843 1107 36-37 0.047425005296567235 1107 38-39 -0.053525385632056555 1107 40-41 0.1383606129831776 1107 42-43 0.05994487812957772 1107 44-45 0.04586125686930842 1107 46-47 -0.12730037107319703 1107 48-49 0.14672074059896545 1107 50-51 0.06468513859062597 1107 52-53 0.32134194192379795 1107 54-55 0.3292154101522229 1107 56-57 0.17063855493908164 1107 58-59 0.34457070353334984 1107 60-61 0.34696870703259464 1107 62-63 0.29728116352075773 1107 64-65 0.3072971415504142 1107 66-67 0.30443285835821143 1107 68-69 0.3335776759973257 1107 70-71 0.46348602411178064 1107 72-73 0.5436827898947207 1107 74-75 0.6987511692452166 1107 76-77 0.4472122829571994 1107 78-79 0.39666486757456454 1107 80-81 0.3475130693135462 1107 82-83 0.32759754968644117 1107 84-85 0.0950805052203556 1107 86-87 0.14344612718371152 1107 88-89 -0.034765545009648235 1107 90-91 -0.04586336038597949 1107 92-93 0.13319642667681109 1107 94-95 0.23687560829377574 1107 96-97 0.13791001014166326 1107 98-99 0.29857862106480226 1107 100-101 0.3197933922022891 1108 1 0.23457016994818147 1108 2 0.27164725642517595 1108 3 0.21943472456980473 1108 4 0.14802146551501494 1108 5 0.07661350365889774 1108 6 0.22150525246021147 1108 7 0.17902565807366244 1108 8 0.15547034595029885 1108 9 0.16537864348938314 1108 10-11 0.24635143360350753 1108 12-13 0.2119959270102285 1108 14-15 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48-49 -2.632466572386079 2316 50-51 -2.4757250687694636 2316 52-53 -2.302900887734218 2316 54-55 -2.151947559562963 2316 56-57 -2.1605839700237013 2316 58-59 -1.7911578523342655 2316 60-61 -2.204918740039112 2316 62-63 -1.9447198129257153 2316 64-65 -2.0876350213783645 2316 66-67 -2.014465274712073 2316 68-69 -1.79225911766396 2316 70-71 -1.6943598431134674 2316 72-73 -1.8348923969829585 2316 74-75 -1.6571815418114895 2316 76-77 -1.9356675552202631 2316 78-79 -2.613062655935529 2316 80-81 -2.5717094476619486 2316 82-83 -2.342469873043992 2316 84-85 -2.0394883956583456 2316 86-87 -2.223001854078099 2316 88-89 -2.67447327001916 2316 90-91 -2.6496472402331435 2316 92-93 -3.099753949275051 2316 94-95 -3.0181631250814256 2316 96-97 -3.2173512691629647 2316 98-99 -4.353054131226298 2316 100-101 -5.269409186495 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1783.0 3 727.0 4 724.0 5 746.0 6 940.0 7 1079.0 8 1402.0 9 1789.0 10 2336.0 11 3334.0 12 4906.0 13 6661.0 14 7998.0 15 10719.0 16 14473.0 17 20736.0 18 14530.0 19 8304.0 20 7600.0 21 7599.0 22 8372.0 23 9038.0 24 10396.0 25 11507.0 26 13753.0 27 15906.0 28 19109.0 29 22905.0 30 27910.0 31 34679.0 32 43454.0 33 56548.0 34 78085.0 35 107650.0 36 147294.0 37 185580.0 38 143371.0 39 22438.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.750017653858016 18.476899462207736 14.517510768853391 20.255572115080856 2 15.728340987920216 21.026498219298105 40.16423525520297 23.080925537578707 3 19.30985204876639 28.14529939148865 29.81928634412808 22.72556221561688 4 12.268059725346662 16.774965085258874 37.62215513388398 33.33482005551049 5 12.578433413377542 39.505256153863314 32.25750693262161 15.65880350013754 6 29.31370052217428 36.55889192721162 17.94297908886257 16.184428461751523 7 27.289991405941517 35.71142545234943 20.080273151696744 16.918309990012308 8 27.735930060104597 32.07018893733436 19.631845400715903 20.562035601845142 9 29.326785911767793 15.824122923998674 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34-35 21.572843621445056 25.71595930092123 31.77576301482365 20.935434062810067 36-37 21.682065431978334 25.441036432131625 31.723175257923515 21.153722877966526 38-39 21.55075265680466 25.68318829565946 31.645170370879615 21.120888676656257 40-41 21.304547166945305 25.938858583939496 31.541653524543296 21.214940724571907 42-43 21.0828777018521 26.297562584487316 30.77582002053245 21.843739693128132 44-45 21.2754838493898 26.047364841764153 30.61513348594533 22.062017822900717 46-47 21.84050926442061 26.56845749069098 29.68762774346511 21.903405501423297 48-49 21.80369051136121 27.043419529497893 28.89327394921134 22.259616009929562 50-51 21.93622412836003 27.298014813988974 28.84101091887146 21.92475013877953 52-53 22.195908940146623 27.283255307071048 28.273409969089897 22.247425783692435 54-55 21.42302677024748 27.19093805730701 28.589585585401444 22.79644958704407 56-57 22.08173054367028 27.443366808237062 27.698249268263904 22.776653379828748 58-59 22.294876958460907 26.869709710881533 28.128253262146558 22.707160068511 60-61 22.0828424796257 27.110036084715468 28.755123769459175 22.051997666199657 62-63 22.283673052267787 27.142412530890574 28.65860569687285 21.915308719968785 64-65 21.972082220415746 27.40150969689931 28.6504703286494 21.975937754035535 66-67 21.96841968819239 27.41508731811726 28.17153925099086 22.444953742699486 68-69 22.046297470986822 27.389889802669032 28.407982463600213 22.155830262743933 70-71 22.192193378523775 27.42830452191008 28.052713401668967 22.326788697897182 72-73 21.913807088959032 27.63317930901837 27.999176761252404 22.4538368407702 74-75 21.673326766165456 28.338382618069623 27.74269092801517 22.245599687749753 76-77 21.658677200959573 27.558615357523365 28.12133951674768 22.661367924769387 78-79 21.563819364529905 27.652346807312128 28.255478238560627 22.528355589597343 80-81 21.757576208371994 28.137778488533183 27.731516692111295 22.373128610983525 82-83 21.69201641072162 27.889722107071226 27.689979012568294 22.728282469638863 84-85 21.521314430535003 27.84263257782537 27.48554530608642 23.150507685553205 86-87 21.53129943712817 27.977447958166753 27.340238947571383 23.1510136571337 88-89 21.51334022510541 27.647683319201327 27.304473069819846 23.534503385873414 90-91 21.54588662593473 27.463944430090574 27.629607668558283 23.360561275416412 92-93 21.70022288209737 27.637287148061638 27.6131264871003 23.04936348274069 94-95 21.29024255288902 27.37889661176718 27.829168135902837 23.50169269944097 96-97 21.1818730466545 27.226297007475853 28.00463854948692 23.58719139638273 98-99 21.131957095586866 27.554281146596278 27.513105550804923 23.800656207011933 100-101 21.334082015044597 27.44451473664981 27.56248788544054 23.658915362865045 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 529.0 1 440.5 2 540.0 3 767.5 4 698.0 5 697.5 6 1118.5 7 2079.0 8 3339.5 9 4007.5 10 3463.0 11 2733.5 12 2679.0 13 2790.5 14 2827.0 15 2900.0 16 2954.0 17 2959.0 18 3049.5 19 3185.0 20 3427.0 21 3715.0 22 4134.0 23 4757.0 24 5335.5 25 6035.0 26 7020.5 27 8283.5 28 9528.5 29 11131.5 30 13185.5 31 15332.5 32 18342.5 33 20435.5 34 21639.5 35 23021.0 36 25450.5 37 27877.5 38 28673.0 39 28911.0 40 28380.0 41 28449.0 42 28764.5 43 29733.0 44 31411.0 45 33267.0 46 34397.0 47 36856.0 48 45203.5 49 45339.5 50 37773.5 51 33910.5 52 31359.0 53 31538.0 54 31904.0 55 30608.5 56 28320.0 57 26148.0 58 25623.0 59 23880.5 60 20806.0 61 18785.5 62 16571.0 63 12100.5 64 8177.0 65 6434.5 66 4708.5 67 3585.0 68 2800.5 69 1983.5 70 1524.0 71 1246.0 72 1059.0 73 1045.5 74 879.0 75 684.0 76 512.0 77 302.0 78 185.5 79 118.5 80 84.5 81 66.5 82 49.0 83 24.0 84 10.0 85 11.5 86 11.5 87 9.5 88 6.0 89 4.5 90 3.5 91 2.5 92 1.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012634849143617358 2 0.011798719420877974 3 0.014400011891622724 4 0.018116143992686653 5 0.029357443598405034 6 0.04663745786835231 7 0.005852908059175687 8 0.024526471867021928 9 0.031680026161569994 10-11 0.03734712761569248 12-13 0.030936799741357206 14-15 0.0261987313125007 16-17 0.027406474245346472 18-19 0.023597438841755948 20-21 0.054162625373006756 22-23 0.012867107399933853 24-25 0.0019509693530585623 26-27 0.001300646235372375 28-29 4.645165126329911E-5 30-31 0.0028335507270612456 32-33 0.0022296792606383573 34-35 0.0 36-37 2.7870990757979466E-4 38-39 9.290330252659822E-5 40-41 0.0013470978866356742 42-43 0.003994842008643723 44-45 0.0018580660505319643 46-47 0.0011148396303191786 48-49 5.574198151595893E-4 50-51 0.0037625837523272278 52-53 0.003809035403590527 54-55 0.0058064564079123885 56-57 8.825813740026831E-4 58-59 0.0059922630129655855 60-61 0.002972905680851143 62-63 7.432264202127857E-4 64-65 0.0012077429328457767 66-67 0.01667614280352438 68-69 0.04222455099833889 70-71 0.053001334091424275 72-73 0.01402839868151633 74-75 0.030472283228724216 76-77 0.02633808626629059 78-79 0.054441335280586554 80-81 0.07896780714760848 82-83 0.0470090710784587 84-85 0.016769046106050976 86-87 0.019556145181848925 88-89 0.020949694719747898 90-91 0.038090354035905265 92-93 0.024015503703125638 94-95 0.013796140425199836 96-97 0.017605175828790363 98-99 0.047194877683511896 100-101 0.06684392616788741 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1076388.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.068999324387356 #Duplication Level Percentage of deduplicated Percentage of total 1 71.93846316818954 33.86071474260978 2 14.417347253061985 13.57220216227665 3 5.521924995531816 7.797344516520141 4 2.6143609794611224 4.922214207038409 5 1.4960975449683205 3.5209907166665735 6 0.9207122266490944 2.600220190446084 7 0.6002546555463352 1.9777370183459573 8 0.4129057077177859 1.5548046782083318 9 0.3139157918720673 1.329813197598677 >10 1.574685698290338 13.389023054905122 >50 0.11590970872145663 3.720722680400321 >100 0.06429409626758462 5.939406325632788 >500 0.006350033893941035 1.9142019854251373 >1k 0.002182824151042231 1.6297897161941652 >5k 3.9687711837131466E-4 1.2607542890245196 >10k+ 1.9843855918565733E-4 1.0100605187073015 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10814 1.0046563135226332 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8404 0.7807593544335314 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5094 0.4732494230704913 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2852 0.26496021880585807 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2026 0.18822209091888797 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1832 0.17019885022872794 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1818 0.16889820399335556 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1685 0.156542064757318 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1336 0.12411881217553522 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1322 0.12281816594016284 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1301 0.12086719658710428 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1185 0.11009041349401888 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 9.290330252659822E-5 0.0 8 0.0 0.0 0.0 9.290330252659822E-5 0.0 9 0.0 0.0 0.0 9.290330252659822E-5 0.0 10-11 0.0 0.0 0.0 9.290330252659822E-5 0.0 12-13 0.0 0.0 0.0 9.290330252659822E-5 0.0 14-15 0.0 0.0 0.0 9.290330252659822E-5 0.0 16-17 0.0 0.0 0.0 9.290330252659822E-5 0.0 18-19 0.0 0.0 0.0 1.8580660505319643E-4 0.0 20-21 0.0 0.0 0.0 1.8580660505319643E-4 0.0 22-23 0.0 0.0 0.0 1.8580660505319643E-4 0.0 24-25 0.0 0.0 0.0 6.503231176861875E-4 0.0 26-27 0.0 0.0 0.0 6.967747689494866E-4 0.0 28-29 0.0 0.0 0.0 0.0013470978866356742 0.0 30-31 0.0 0.0 0.0 0.002183227609375058 0.0 32-33 0.0 0.0 0.0 0.00311226063464104 0.0 34-35 0.0 9.290330252659822E-5 0.0 0.004273551916223518 0.0 36-37 0.0 9.290330252659822E-5 0.0 0.0058064564079123885 0.0 38-39 0.0 9.290330252659822E-5 0.0 0.008547103832447035 0.0 40-41 0.0 9.290330252659822E-5 0.0 0.012123880979721068 0.0 42-43 0.0 9.290330252659822E-5 0.0 0.01700130436236747 0.0 44-45 0.0 9.290330252659822E-5 0.0 0.029589701854721533 0.0 46-47 0.0 9.290330252659822E-5 0.0 0.06150198627260802 0.0 48-49 0.0 9.290330252659822E-5 0.0 0.08616781309341984 0.0 50-51 0.0 9.290330252659822E-5 0.0 0.12077429328457769 0.0 52-53 0.0 9.290330252659822E-5 0.0 0.16039755181217183 0.0 54-55 0.0 9.290330252659822E-5 0.0 0.20508404032746558 0.0 56-57 0.0 9.290330252659822E-5 0.0 0.25246472461603064 0.0 58-59 0.0 9.290330252659822E-5 0.0 0.32497575223804054 0.0 60-61 0.0 9.290330252659822E-5 0.0 0.4194119592563276 0.0 62-63 0.0 9.290330252659822E-5 0.0 0.5345656027380461 0.0 64-65 0.0 9.290330252659822E-5 0.0 0.6272366470083279 0.0 66-67 0.0 9.290330252659822E-5 0.0 0.7502870712048072 0.0 68-69 0.0 9.290330252659822E-5 0.0 0.90929107347908 0.0 70-71 0.0 9.290330252659822E-5 0.0 1.0384266639910515 0.0 72-73 0.0 9.290330252659822E-5 0.0 1.2268345615149927 0.0 74-75 0.0 9.290330252659822E-5 0.0 1.384677272507683 0.0 76-77 0.0 9.290330252659822E-5 0.0 1.530953522335812 0.0 78-79 0.0 9.290330252659822E-5 0.0 1.719825936372386 0.0 80-81 0.0 1.8580660505319643E-4 0.0 1.9232841689056364 0.0 82-83 0.0 1.8580660505319643E-4 0.0 2.1674340479455365 0.0 84-85 0.0 1.8580660505319643E-4 0.0 2.492270445229787 0.0 86-87 0.0 1.8580660505319643E-4 0.0 2.8868307710602497 0.0 88-89 0.0 1.8580660505319643E-4 0.0 3.343636309583533 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14500 0.0 28.865936 1 GGTATCA 10155 0.0 26.620125 1 GTACATG 11210 0.0 25.937227 1 TCGACCG 75 1.0581825E-6 25.340597 18-19 TACATGG 11420 0.0 25.252266 2 ACATGGG 11340 0.0 24.509857 3 TAAGGTG 490 0.0 24.242771 5 ATGGGAG 2445 0.0 24.09801 5 ATCAACG 17460 0.0 23.946188 3 TCAACGC 17505 0.0 23.859705 4 TATCAAC 17720 0.0 23.67417 2 GAGTACT 8370 0.0 23.641628 12-13 CAACGCA 17640 0.0 23.623234 5 CATGGGC 1330 0.0 23.579285 4 AACGCAG 18055 0.0 23.212912 6 CATGGGG 3400 0.0 22.779648 4 AGTACTT 8545 0.0 22.629251 12-13 ATACCGT 105 0.0021912893 22.627638 6 GTACTTT 8900 0.0 22.050507 14-15 CATGGGA 7325 0.0 21.73079 4 >>END_MODULE