FastQCFastQC Report
Thu 26 May 2016
SRR936411_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936411_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076388
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115581.0737763706024221No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97980.9102665581556093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62820.58361854647209No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34120.3169860682207531No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24090.2238040557865751No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22380.2079175910545268No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18410.17103497995146733No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16430.15264012605120086No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15790.1466943146894986No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14180.13173688298271627No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12810.1190091305365723No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG12230.11362073899002963No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11350.10544524836768898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG3550.029.4471021
GTATCAA158550.026.463221
GGTATCA114900.025.309271
GTACATG111750.025.044711
AGGTAAG4400.024.833772
TACATGG112850.024.501292
GTAAGGT5150.023.9802074
TATAGGG2602.3646862E-1123.7540422
ATGGGAG25800.023.7496285
ACATGGG113400.023.7088453
AGTACTT99500.023.51093912-13
GAGTACT97250.023.24899312-13
GTATTAC1050.002191591822.6271021
TAAGGTG5500.022.4541955
ACCGTGC1703.1503769E-622.3515518
CATGGGA74700.022.1281684
GTACTTT102400.021.9869614-15
ATGGGAT23400.021.9227335
ATCAACG195650.021.5352923
TCAACGC197000.021.4590574
Produced by FastQC (version 0.11.5)