##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936411_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1076388 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.915078020193462 31.0 31.0 34.0 30.0 34.0 2 32.005224881734094 31.0 31.0 34.0 30.0 34.0 3 32.08905246063687 33.0 31.0 34.0 30.0 34.0 4 35.86116437567123 37.0 35.0 37.0 35.0 37.0 5 35.7435469366065 37.0 35.0 37.0 35.0 37.0 6 35.82232429198393 37.0 35.0 37.0 35.0 37.0 7 35.7438851046277 37.0 35.0 37.0 33.0 37.0 8 35.725734586413076 37.0 35.0 37.0 33.0 37.0 9 37.30088778395894 39.0 37.0 39.0 34.0 39.0 10-11 37.20778706191447 39.0 37.0 39.0 33.0 39.0 12-13 37.07048666465995 39.0 37.0 39.0 33.0 39.0 14-15 38.05832051267758 40.0 37.0 41.0 33.0 41.0 16-17 38.06102539233065 40.0 37.0 41.0 33.0 41.0 18-19 38.291052111320454 40.0 38.0 41.0 33.5 41.0 20-21 38.33986490001746 40.0 38.0 41.0 34.0 41.0 22-23 38.234932942396235 40.0 37.5 41.0 33.5 41.0 24-25 38.09566160157861 40.0 37.0 41.0 33.0 41.0 26-27 37.85386589222473 40.0 37.0 41.0 33.0 41.0 28-29 37.80635189169705 40.0 37.0 41.0 33.0 41.0 30-31 37.561688721910684 39.0 36.5 41.0 33.0 41.0 32-33 37.23900024898085 39.0 35.5 41.0 32.0 41.0 34-35 36.98653505984831 39.0 35.0 41.0 31.5 41.0 36-37 36.7291181246911 39.0 35.0 41.0 31.0 41.0 38-39 36.45823113970056 39.0 35.0 40.5 31.0 41.0 40-41 36.13201791547286 38.5 35.0 40.0 30.0 41.0 42-43 35.735547033225934 38.0 35.0 40.0 29.5 41.0 44-45 35.227266097355226 38.0 35.0 40.0 27.0 41.0 46-47 34.55780071869995 38.0 34.0 40.0 23.5 41.0 48-49 33.935519998364896 38.0 33.5 40.0 21.0 41.0 50-51 33.53431104768912 38.0 33.0 40.0 18.5 41.0 52-53 33.236174130517995 37.5 33.0 40.0 16.0 41.0 54-55 32.886047131703435 37.0 33.0 40.0 12.5 41.0 56-57 32.53433148641568 36.5 32.0 40.0 8.5 41.0 58-59 32.157600233373095 36.0 31.5 39.0 7.5 40.5 60-61 31.76042467957651 35.5 31.0 39.0 6.0 40.0 62-63 31.428258676239423 35.0 31.0 39.0 2.0 40.0 64-65 31.109873484282616 35.0 30.5 38.5 2.0 40.0 66-67 30.659960441773784 35.0 30.0 38.0 2.0 40.0 68-69 30.22231388681405 35.0 29.0 37.5 2.0 40.0 70-71 29.723472390996555 34.0 29.0 37.0 2.0 39.0 72-73 29.18699623184205 34.0 28.5 36.0 2.0 39.0 74-75 28.673095110684994 34.0 27.5 35.5 2.0 38.5 76-77 27.318840882655696 31.5 25.5 34.5 2.0 36.0 78-79 28.07368068020082 33.0 27.0 35.0 2.0 37.0 80-81 27.93405026811893 33.0 27.0 35.0 2.0 36.5 82-83 27.540305633284653 33.0 26.0 35.0 2.0 36.0 84-85 27.100002508389167 33.0 26.0 35.0 2.0 36.0 86-87 26.6942858894748 33.0 25.0 35.0 2.0 35.0 88-89 26.199579055136255 32.0 24.5 34.5 2.0 35.0 90-91 25.61913733709406 32.0 22.5 34.0 2.0 35.0 92-93 25.068243978936962 31.5 19.5 34.0 2.0 35.0 94-95 24.362072969969937 31.0 15.0 34.0 2.0 35.0 96-97 23.578817303797514 31.0 2.0 34.0 2.0 35.0 98-99 22.534305473490974 31.0 2.0 34.0 2.0 35.0 100-101 19.047988271887093 24.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2817382357083211 1101 2 0.3905281454749101 1101 3 0.37974513867931137 1101 4 0.1799131488014325 1101 5 0.2953029898798647 1101 6 0.24996330431984148 1101 7 0.21240528889727273 1101 8 0.1846668109596692 1101 9 0.19932533744797354 1101 10-11 0.2408785008312151 1101 12-13 0.3210546525634044 1101 14-15 0.29369263868317574 1101 16-17 0.37378278161470746 1101 18-19 0.3221298450731851 1101 20-21 0.30516463574902986 1101 22-23 0.3721952520106555 1101 24-25 0.33529377930759097 1101 26-27 0.212840604979597 1101 28-29 0.2353648783727067 1101 30-31 0.3600383812549879 1101 32-33 0.2766314880227867 1101 34-35 0.33741127412642413 1101 36-37 0.23089957428260277 1101 38-39 0.18754868730285779 1101 40-41 0.11142825643703702 1101 42-43 0.15193714540784242 1101 44-45 0.43245736760330544 1101 46-47 0.24310429133953448 1101 48-49 0.2692098935248026 1101 50-51 0.24835779404016733 1101 52-53 0.09833288210466407 1101 54-55 0.07669876528250086 1101 56-57 0.09183240052264097 1101 58-59 -0.00310246534003511 1101 60-61 -0.13123172158201513 1101 62-63 -0.1685049798219751 1101 64-65 0.02799424034088105 1101 66-67 -0.08051934808296224 1101 68-69 0.010096162673029596 1101 70-71 -0.07597220776824187 1101 72-73 0.0792944124811008 1101 74-75 0.17830056493662383 1101 76-77 -0.0011848352855956534 1101 78-79 0.06609800616180195 1101 80-81 0.21998603314196785 1101 82-83 0.039061654459931816 1101 84-85 -0.017131738117054596 1101 86-87 -0.10211306950073151 1101 88-89 -0.4484211909805502 1101 90-91 -0.21898389675065744 1101 92-93 -0.15902813800386895 1101 94-95 -0.46675526089413566 1101 96-97 -0.18319361470795315 1101 98-99 -0.3541259072289442 1101 100-101 0.04627743639837334 1102 1 0.1913971805014434 1102 2 0.41547167362742954 1102 3 0.33673478956166747 1102 4 0.19715704462912242 1102 5 0.10171263144869869 1102 6 0.23624186169871564 1102 7 0.28430211743832245 1102 8 0.2478478614402917 1102 9 0.31533762572198043 1102 10-11 0.39799812384254096 1102 12-13 0.2853102319819314 1102 14-15 0.3837640190983862 1102 16-17 0.4581218351117897 1102 18-19 0.34625010056920047 1102 20-21 0.26674605598992684 1102 22-23 0.3951356802931443 1102 24-25 0.31498824054879293 1102 26-27 0.2609487112092239 1102 28-29 0.4692253981222194 1102 30-31 0.503774697691945 1102 32-33 0.493477474525136 1102 34-35 0.5620983332116083 1102 36-37 0.5016089301907769 1102 38-39 0.5698808182202484 1102 40-41 0.5396850313211345 1102 42-43 0.773065956823153 1102 44-45 0.8674407214402677 1102 46-47 0.748381020768143 1102 48-49 0.45469923511519283 1102 50-51 0.40907354429906206 1102 52-53 0.4843848570554954 1102 54-55 0.7122339793889907 1102 56-57 0.6933739950791065 1102 58-59 0.46937054980484305 1102 60-61 0.4323458481476834 1102 62-63 0.2965665743527701 1102 64-65 0.4325015625675057 1102 66-67 0.5191136717881797 1102 68-69 0.5466228486617588 1102 70-71 0.4351550050593822 1102 72-73 0.2324474529253706 1102 74-75 0.3706339469226272 1102 76-77 0.5778264034808487 1102 78-79 0.7341022736958749 1102 80-81 0.7746320154740971 1102 82-83 0.8715320979637582 1102 84-85 0.6477076733246641 1102 86-87 1.043905057093511 1102 88-89 0.958054131690897 1102 90-91 0.869282469659538 1102 92-93 0.9519680601375988 1102 94-95 0.9392349251675682 1102 96-97 0.9282366347412285 1102 98-99 0.8786757219404144 1102 100-101 0.6792201583066664 1103 1 0.22053242815448826 1103 2 0.31363064579489475 1103 3 0.2938742003776156 1103 4 0.12243723100647941 1103 5 0.08413085011125077 1103 6 0.15653659157561606 1103 7 0.15806798777371256 1103 8 0.1963197600907094 1103 9 0.11898315032186702 1103 10-11 0.23652116598422168 1103 12-13 0.2461361786879408 1103 14-15 0.32554556332854645 1103 16-17 0.3729737809079623 1103 18-19 0.27781914428241095 1103 20-21 0.2252836416311581 1103 22-23 0.1643224160701564 1103 24-25 0.2384815064260195 1103 26-27 0.13436753642530874 1103 28-29 0.29044787920187787 1103 30-31 0.40870363443116986 1103 32-33 0.43092733046867693 1103 34-35 0.6356202036021514 1103 36-37 0.5462157732174759 1103 38-39 0.29537881245798303 1103 40-41 0.36539187296930464 1103 42-43 0.736096174217451 1103 44-45 0.774465024937598 1103 46-47 0.7822604820383177 1103 48-49 0.9761826031415453 1103 50-51 0.8907335710425599 1103 52-53 0.7490748254871349 1103 54-55 0.7820520822549568 1103 56-57 0.6582455849386264 1103 58-59 0.5063961629064053 1103 60-61 0.3660205975997144 1103 62-63 0.5330287130053932 1103 64-65 0.454512907875678 1103 66-67 0.33941253725735976 1103 68-69 0.5717200350804745 1103 70-71 0.1819244182188342 1103 72-73 0.34280952402998466 1103 74-75 0.3178820548891572 1103 76-77 0.397454958367053 1103 78-79 0.2646370747049609 1103 80-81 0.5160434241946845 1103 82-83 0.23603906643675643 1103 84-85 0.4904803811726346 1103 86-87 0.42238998285962737 1103 88-89 0.34601611181248515 1103 90-91 0.10717207691612529 1103 92-93 0.5918782488584604 1103 94-95 0.6313028591635153 1103 96-97 0.7849228535692028 1103 98-99 0.782219579896605 1103 100-101 0.8363302753439292 1104 1 0.2039211324734289 1104 2 0.26047449961549063 1104 3 0.20085094456366193 1104 4 0.1838990250264132 1104 5 0.16884845808467475 1104 6 0.13826416632644367 1104 7 0.12733709076374566 1104 8 0.11741610560566329 1104 9 0.1422389642753501 1104 10-11 0.18627199654901005 1104 12-13 0.2025315275251458 1104 14-15 0.2507947327637581 1104 16-17 0.21890401346610133 1104 18-19 0.02075934361795362 1104 20-21 0.10983513664776723 1104 22-23 0.04762806391068608 1104 24-25 0.17868084197419165 1104 26-27 0.19582933044524253 1104 28-29 0.36229173302248086 1104 30-31 0.39873685702253425 1104 32-33 0.5065087258175112 1104 34-35 0.44915840958221764 1104 36-37 0.2850031486327609 1104 38-39 0.5719568855476851 1104 40-41 0.5261061586835893 1104 42-43 0.5172423536194515 1104 44-45 0.7071892774292863 1104 46-47 0.6427255983173907 1104 48-49 0.8225281180917179 1104 50-51 0.850451179015991 1104 52-53 0.9720814700054135 1104 54-55 0.9813876304277187 1104 56-57 1.10384359158315 1104 58-59 1.1679410134047359 1104 60-61 0.851070431486761 1104 62-63 1.067081869151572 1104 64-65 1.1276856653507572 1104 66-67 0.9955587166593567 1104 68-69 0.820058905512365 1104 70-71 0.8156453484513975 1104 72-73 0.9894042084153654 1104 74-75 1.0329983339589255 1104 76-77 0.7325878487325035 1104 78-79 0.7077433869973184 1104 80-81 1.0152128594106316 1104 82-83 1.0537300963370946 1104 84-85 1.0959621087473899 1104 86-87 1.1474730393380348 1104 88-89 0.8663649490217864 1104 90-91 0.7616571267500127 1104 92-93 0.7899679498551357 1104 94-95 0.931552028598734 1104 96-97 1.0021155445991035 1104 98-99 0.6057245632853103 1104 100-101 0.7362472188655254 1105 1 0.18105215691251075 1105 2 0.23926096482279036 1105 3 0.2080074548262587 1105 4 0.182929084645032 1105 5 0.0929513343418975 1105 6 0.10121585703777214 1105 7 0.21026272677157465 1105 8 0.07278462606258529 1105 9 0.1653383362318479 1105 10-11 0.1848341523766166 1105 12-13 0.0870005349433356 1105 14-15 0.17998339612334036 1105 16-17 0.33725238419381043 1105 18-19 0.1330974972610619 1105 20-21 0.03809225902522684 1105 22-23 0.040453065048446035 1105 24-25 0.10246941203745052 1105 26-27 0.2678509592624252 1105 28-29 0.08722119190383637 1105 30-31 0.2304650006533535 1105 32-33 0.05342283631676281 1105 34-35 0.17361569807263777 1105 36-37 0.4156891041235866 1105 38-39 0.3583950710480721 1105 40-41 0.14720933075841458 1105 42-43 0.4238109264133314 1105 44-45 0.43560739284366434 1105 46-47 0.38858841859682514 1105 48-49 0.25910084370951836 1105 50-51 0.30803833143031056 1105 52-53 0.3584505365871209 1105 54-55 0.23257374520493812 1105 56-57 0.3458565165365002 1105 58-59 0.42092621114742457 1105 60-61 0.05335846680283041 1105 62-63 0.08159684207288365 1105 64-65 -0.03876435285340207 1105 66-67 0.031248824974102973 1105 68-69 -0.058232065224444796 1105 70-71 -0.166228712896622 1105 72-73 0.008911103240833995 1105 74-75 0.20625784563234006 1105 76-77 -0.07858381649300483 1105 78-79 0.07636624231976086 1105 80-81 0.17094660082054247 1105 82-83 0.2512893167439536 1105 84-85 0.3222270134031042 1105 86-87 0.1793900511252282 1105 88-89 0.26878212009537705 1105 90-91 0.17236999026432542 1105 92-93 0.3096042648781214 1105 94-95 0.2806239008965541 1105 96-97 0.2537140973684089 1105 98-99 0.15741868789278257 1105 100-101 0.028057053662774223 1106 1 0.0152795338450602 1106 2 0.1169826217585026 1106 3 0.051354076470772725 1106 4 0.01636664464006543 1106 5 0.1110677162321494 1106 6 0.16346458373146788 1106 7 0.1250994273554369 1106 8 0.1417626777627703 1106 9 0.20427934629407218 1106 10-11 0.11881103240914115 1106 12-13 0.10956784230987893 1106 14-15 0.17664131998470367 1106 16-17 0.31273523831977457 1106 18-19 0.21692361330980248 1106 20-21 0.17018049548187975 1106 22-23 -0.008249842381339079 1106 24-25 0.03791722906530737 1106 26-27 -0.032761201800937556 1106 28-29 0.3319500990625812 1106 30-31 0.04559207718724423 1106 32-33 0.05776682947309553 1106 34-35 -0.08271836555314849 1106 36-37 0.11277701590125133 1106 38-39 0.16262881451213929 1106 40-41 -0.2061585785347475 1106 42-43 -0.11226549737462932 1106 44-45 -0.027001233453638918 1106 46-47 -0.0960764985676903 1106 48-49 -0.1890994465252689 1106 50-51 -0.284124623543768 1106 52-53 -0.25262567545366466 1106 54-55 -0.29723672696301406 1106 56-57 -0.11136067394560456 1106 58-59 -0.15522195258113314 1106 60-61 -0.13043666357695827 1106 62-63 -0.07820945161761372 1106 64-65 0.019877260829421317 1106 66-67 -0.3161037001814613 1106 68-69 -0.29982076187828 1106 70-71 -0.35411373983086136 1106 72-73 -0.37060899297707905 1106 74-75 -0.46769562927985575 1106 76-77 -0.5517385105042294 1106 78-79 -0.3983059730638132 1106 80-81 -0.03309649960972649 1106 82-83 -0.09502282399168394 1106 84-85 -0.11740916261243584 1106 86-87 -0.13875512837990556 1106 88-89 -0.318821212522721 1106 90-91 -0.028370207253434643 1106 92-93 -0.18374087453621613 1106 94-95 -0.3422970948233335 1106 96-97 -0.3900664648062069 1106 98-99 -0.09983577009093025 1106 100-101 -0.13222637698014594 1107 1 0.018064347230044575 1107 2 0.10684321851196898 1107 3 0.030448670030949643 1107 4 -0.037393598575548026 1107 5 -0.11766158649420078 1107 6 -0.02457839899266645 1107 7 -0.07573838757878093 1107 8 -0.14699354505210493 1107 9 -0.08570636722714653 1107 10-11 -0.005026455338295932 1107 12-13 0.019892583659590457 1107 14-15 0.12093760082612448 1107 16-17 0.14646948271814608 1107 18-19 -0.027874421672763106 1107 20-21 -0.14570344059544738 1107 22-23 -0.15022993885740732 1107 24-25 -0.10952343191973313 1107 26-27 -0.04854610121640235 1107 28-29 0.05343048716165555 1107 30-31 0.06054275191684866 1107 32-33 -0.07618194919893284 1107 34-35 -0.0108219489597019 1107 36-37 0.20663796219587738 1107 38-39 0.15657348810776028 1107 40-41 0.10516370993018853 1107 42-43 0.25962258713304465 1107 44-45 0.06572573628869094 1107 46-47 0.0647405471185678 1107 48-49 0.07270112398551731 1107 50-51 -0.07081476004771758 1107 52-53 0.0712865301036274 1107 54-55 0.05304585267784745 1107 56-57 0.287075942252109 1107 58-59 0.07582407643484856 1107 60-61 0.06732302145665159 1107 62-63 -0.001629793151842307 1107 64-65 -0.03875555905323935 1107 66-67 0.2354834266782717 1107 68-69 0.18745756568920768 1107 70-71 -0.0909949385131732 1107 72-73 0.10261080270392142 1107 74-75 0.16410916146624643 1107 76-77 0.049894307346217914 1107 78-79 0.2594376845592663 1107 80-81 0.30716115828837687 1107 82-83 0.5080179808742535 1107 84-85 0.368841541042368 1107 86-87 0.5810043114329702 1107 88-89 0.531369917396173 1107 90-91 0.4674508791557166 1107 92-93 0.48366698648188233 1107 94-95 0.44293458796671104 1107 96-97 0.40900698015901327 1107 98-99 0.6388222628187741 1107 100-101 0.46605091347975147 1108 1 0.002089096911834787 1108 2 0.1895300248912264 1108 3 0.029919616454286313 1108 4 0.0485183346647986 1108 5 0.1465170154901898 1108 6 0.10658082445694106 1108 7 0.1770367727799993 1108 8 0.24646177092023436 1108 9 0.05258932156200302 1108 10-11 0.039256209655633256 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2316 52-53 -1.9362374991657987 2316 54-55 -1.9380692831410684 2316 56-57 -1.8522389078214303 2316 58-59 -2.0207677459756965 2316 60-61 -1.6033040109348136 2316 62-63 -1.573350186006813 2316 64-65 -1.456156973403008 2316 66-67 -1.5016438038978812 2316 68-69 -1.8101222847687168 2316 70-71 -1.8459353283529367 2316 72-73 -1.6957340306661877 2316 74-75 -2.043791568472418 2316 76-77 -1.9688054413536058 2316 78-79 -2.0577446783014217 2316 80-81 -1.9497101594451074 2316 82-83 -1.6552467649151943 2316 84-85 -1.8489314820778482 2316 86-87 -1.9599650063823866 2316 88-89 -2.44525377431032 2316 90-91 -2.4513842541472 2316 92-93 -2.274504263060095 2316 94-95 -2.2795734018215335 2316 96-97 -1.978228428306121 2316 98-99 -1.6643139474706565 2316 100-101 -1.114410572296375 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 23.0 5 40.0 6 125.0 7 311.0 8 607.0 9 1247.0 10 2055.0 11 3397.0 12 4949.0 13 7001.0 14 9135.0 15 12708.0 16 16623.0 17 22140.0 18 17949.0 19 10137.0 20 8324.0 21 8488.0 22 8952.0 23 10032.0 24 11507.0 25 13347.0 26 15534.0 27 19031.0 28 23008.0 29 28011.0 30 34294.0 31 41932.0 32 51921.0 33 65885.0 34 87702.0 35 115522.0 36 155343.0 37 178247.0 38 87489.0 39 3361.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.484336731470364 19.466103490182814 15.283626133073671 19.765933645273144 2 16.02989601064423 21.998877598804754 39.01970148588079 22.95152490467023 3 19.606050555080177 27.97039200766307 30.37039721052353 22.053160226733226 4 12.697849379451814 17.476242280889682 36.94795645060745 32.877951889051054 5 12.511671508697539 39.930356514072244 32.05189071472835 15.50608126250187 6 29.32947970434453 37.174977796110696 17.178749670193277 16.3167928293515 7 26.930487761825216 36.05806838810612 19.827924183332 17.183519666736654 8 28.146507514158724 32.247428388803826 19.187675590504362 20.41838850653308 9 29.00914911723282 15.795884012084862 20.02605008602846 35.16891678465386 10-11 25.366364173513638 27.469416232808243 25.136939467924208 22.027280125753908 12-13 27.95432502034582 25.434601649219424 26.602814226840138 20.008259103594614 14-15 24.27477022583969 25.527623531772615 27.576568935035205 22.621037307352495 16-17 20.928498662842635 29.064184947189602 27.922363579521726 22.08495281044604 18-19 20.878754362485942 25.928762979455378 33.029099343205765 20.163383314852915 20-21 22.597125092613453 24.30488232575316 33.13236697921046 19.965625602422932 22-23 21.948806278811798 24.652632615558417 33.03316108965033 20.365400015979457 24-25 21.535241356597016 25.021263312505894 33.26859500485189 20.1749003260452 26-27 21.03754697436326 25.058180855363087 33.53920762552433 20.365064544749323 28-29 20.78878619977183 25.56629208055088 33.37063400929776 20.27428771037953 30-31 21.013660653897894 25.232167941314188 33.11259262562525 20.64157877916267 32-33 20.669284754700573 25.479812478747775 32.94136337538254 20.909539391169112 34-35 21.39282313320172 25.307351062594357 32.82406224596446 20.47576355823946 36-37 21.544608947059395 25.171362051922376 32.63399592707381 20.65003307394442 38-39 21.38858448612814 25.30939527572077 32.66445762396135 20.637562614189747 40-41 21.287464452840847 25.582852635715593 32.44966726651288 20.68001564493068 42-43 21.08175953384319 25.96286056173679 31.567361219499507 21.388018684920514 44-45 21.272571017118437 25.813346340556986 31.3843866861775 21.529695956147073 46-47 21.934005210017204 26.40042438228594 30.254610790904398 21.410959616792457 48-49 22.030523018149925 26.70796604878629 29.407321659506785 21.854189273556997 50-51 21.989119967892247 27.136673029068387 29.28666622382245 21.58754077921692 52-53 22.28751821498067 27.19256805562717 28.679356417177754 21.840557312214408 54-55 21.500959926494666 27.12311446327962 28.90240429883559 22.473521311390122 56-57 22.25932574414551 27.290925680220912 28.086034333455977 22.363714242177597 58-59 22.423222158582305 26.655369420416893 28.470454124214896 22.450954296785905 60-61 22.176532808348963 26.9382760896902 29.102768199362757 21.782422902598086 62-63 22.483637360238205 26.925170826423635 29.023165503049558 21.568026310288605 64-65 22.13035365141568 27.227781873384888 28.90877697119867 21.733087504000764 66-67 22.151615023539602 27.281646076771395 28.436306035754182 22.130432863934818 68-69 22.36520729299733 27.189780513296945 28.59342701196144 21.851585181744284 70-71 22.330113323593558 27.31793086308494 28.26386582337871 22.08808998994279 72-73 22.16091496971051 27.48165466406098 28.213749351404054 22.143681014824455 74-75 21.887124827186373 28.21261465162259 27.788385065929326 22.11187545526171 76-77 21.831087979887975 27.58403004168637 28.21819800050354 22.36668397792211 78-79 21.756651982296997 27.45693754009632 28.393699053575883 22.3927114240308 80-81 21.79084052229713 27.996521611486113 27.934228355648504 22.278409510568252 82-83 21.995418821110587 27.726816433713424 27.660899856507182 22.616864888668804 84-85 21.595608189400085 27.744997756341473 27.56406504472917 23.095329009529276 86-87 21.55963579759741 27.889512945945178 27.25349191432947 23.297359342127937 88-89 21.595529021780735 27.54444593093043 27.338799885540578 23.521225161748255 90-91 21.586886001362984 27.373525699481238 27.542667554262668 23.49692074489311 92-93 21.6388267324686 27.586921308500905 27.524442299357606 23.249809659672888 94-95 21.216528511007937 27.360441107134314 27.764015115444558 23.659015266413196 96-97 21.134873272515538 27.085674310620416 27.821006341135536 23.958446075728514 98-99 21.032229354158712 27.25266843741842 27.44302889595579 24.272073312467075 100-101 21.0538295631751 27.34121507530639 27.46106239539489 24.143892966123623 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1170.0 1 869.0 2 760.5 3 961.0 4 830.5 5 801.0 6 1249.5 7 2314.0 8 3495.0 9 4101.5 10 3662.5 11 2869.0 12 2708.5 13 2849.0 14 2964.0 15 3011.5 16 3088.0 17 3243.5 18 3423.0 19 3545.5 20 3680.5 21 3988.5 22 4510.5 23 5087.0 24 5623.0 25 6315.5 26 7310.5 27 8668.5 28 10032.0 29 11644.0 30 13761.5 31 15663.0 32 18330.5 33 20300.5 34 21345.5 35 22881.5 36 25551.5 37 27861.5 38 28566.0 39 28712.5 40 28165.0 41 28381.0 42 28726.0 43 29442.5 44 30981.5 45 32780.0 46 33661.5 47 36073.0 48 45057.0 49 45102.5 50 37123.5 51 33152.5 52 30593.5 53 31096.5 54 31600.5 55 30446.5 56 28368.0 57 26288.5 58 25540.0 59 23774.0 60 20718.5 61 18455.5 62 16037.5 63 11910.0 64 8220.0 65 6475.0 66 4727.0 67 3521.5 68 2806.5 69 2006.5 70 1447.0 71 1186.5 72 1088.5 73 1065.5 74 873.5 75 700.0 76 563.0 77 365.5 78 232.5 79 164.5 80 109.0 81 74.5 82 53.0 83 28.5 84 15.5 85 12.5 86 6.0 87 3.0 88 1.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010405169882979 2 0.011520009513298178 3 0.005295488244016098 4 9.290330252659822E-5 5 0.004552261823803312 6 0.0 7 1.8580660505319643E-4 8 0.0018580660505319643 9 0.0 10-11 0.0 12-13 0.0 14-15 4.6451651263299107E-4 16-17 0.003483873844747433 18-19 0.002183227609375058 20-21 5.109681638962902E-4 22-23 5.574198151595893E-4 24-25 3.2516155884309373E-4 26-27 2.7870990757979466E-4 28-29 0.0 30-31 0.0011148396303191786 32-33 0.0027870990757979464 34-35 0.0012077429328457767 36-37 0.0011148396303191786 38-39 0.005063229987699603 40-41 9.290330252659822E-5 42-43 0.0011148396303191786 44-45 0.005852908059175687 46-47 0.0 48-49 0.0018580660505319643 50-51 0.0011612912815824777 52-53 6.967747689494866E-4 54-55 3.2516155884309373E-4 56-57 0.011148396303191786 58-59 0.001811614399268665 60-61 0.003576777147274031 62-63 2.7870990757979466E-4 64-65 0.0029264540295878437 66-67 9.754846765292812E-4 68-69 0.0012077429328457767 70-71 0.004227100264960219 72-73 0.002276130911901656 74-75 0.007803877412234249 76-77 0.0015793561429521697 78-79 0.006828392735704968 80-81 0.0025548408194814506 82-83 0.0025548408194814506 84-85 0.0019509693530585623 86-87 7.896780714760849E-4 88-89 0.0022296792606383573 90-91 0.0067354894331783715 92-93 0.0024619375169548526 94-95 5.109681638962902E-4 96-97 3.2516155884309373E-4 98-99 8.361297227393839E-4 100-101 0.001718711096742067 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1076388.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.24620362070574 #Duplication Level Percentage of deduplicated Percentage of total 1 70.96999420753339 32.11122808874363 2 14.710654178914961 13.312025087459444 3 5.628655383980676 7.640258628431142 4 2.82585343945392 5.114365604952149 5 1.598747387901573 3.6168624925532993 6 0.9947766861729781 2.700592109982808 7 0.6172163940416712 1.9548689049993047 8 0.42988947736957117 1.5560693461969914 9 0.3353131937047366 1.3654484135166294 >10 1.680973496308499 13.782832654733458 >50 0.12872354802189098 4.005366925100268 >100 0.06930228105041046 6.2530387663157025 >500 0.007012730594909665 2.2174564438584192 >1k 0.00226882460423548 1.7903154083994746 >5k 4.125135644064509E-4 1.500639687607566 >10k+ 2.0625678220322546E-4 1.0786314371497667 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11558 1.0737763706024221 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9798 0.9102665581556093 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6282 0.58361854647209 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3412 0.3169860682207531 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2409 0.2238040557865751 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2238 0.2079175910545268 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1841 0.17103497995146733 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1643 0.15264012605120086 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1579 0.1466943146894986 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1418 0.13173688298271627 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1281 0.1190091305365723 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1223 0.11362073899002963 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1135 0.10544524836768898 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 9.290330252659822E-5 0.0 0.0 0.0 0.0 16-17 9.290330252659822E-5 0.0 0.0 0.0 0.0 18-19 9.290330252659822E-5 0.0 0.0 0.0 0.0 20-21 9.290330252659822E-5 0.0 0.0 0.0 0.0 22-23 9.290330252659822E-5 0.0 0.0 0.0 0.0 24-25 9.290330252659822E-5 0.0 0.0 4.6451651263299107E-4 0.0 26-27 9.290330252659822E-5 0.0 0.0 6.038714664228883E-4 0.0 28-29 9.290330252659822E-5 0.0 0.0 0.001254194584109076 0.0 30-31 9.290330252659822E-5 0.0 0.0 0.002183227609375058 0.0 32-33 9.290330252659822E-5 0.0 0.0 0.00311226063464104 0.0 34-35 9.290330252659822E-5 0.0 0.0 0.004320003567486817 0.0 36-37 9.290330252659822E-5 0.0 0.0 0.005945811361702285 0.0 38-39 9.290330252659822E-5 0.0 0.0 0.00887226539129013 0.0 40-41 9.290330252659822E-5 0.0 0.0 0.012634849143617358 0.0 42-43 9.290330252659822E-5 0.0 0.0 0.01755872417752706 0.0 44-45 9.290330252659822E-5 0.0 0.0 0.030425831577460916 0.0 46-47 9.290330252659822E-5 0.0 0.0 0.0628026325079804 0.0 48-49 9.290330252659822E-5 0.0 0.0 0.08816523409774171 0.0 50-51 9.290330252659822E-5 0.0 0.0 0.12300397254521603 0.0 52-53 9.290330252659822E-5 0.0 0.0 0.16234852116523038 0.0 54-55 9.290330252659822E-5 0.0 0.0 0.2073137195881039 0.0 56-57 9.290330252659822E-5 0.0 0.0 0.2552053720405653 0.0 58-59 9.290330252659822E-5 0.0 0.0 0.32757704470878535 0.0 60-61 9.290330252659822E-5 0.0 0.0 0.422570671542232 0.0 62-63 9.290330252659822E-5 0.0 0.0 0.5385139930954266 0.0 64-65 9.290330252659822E-5 0.0 0.0 0.6315101989245513 0.0 66-67 9.290330252659822E-5 0.0 0.0 0.7553503011925068 0.0 68-69 9.290330252659822E-5 0.0 0.0 0.9142614001642531 0.0 70-71 9.290330252659822E-5 0.0 0.0 1.0433505390249613 0.0 72-73 9.290330252659822E-5 0.0 0.0 1.2318977915026923 0.0 74-75 9.290330252659822E-5 0.0 0.0 1.3896011475415928 0.0 76-77 9.290330252659822E-5 0.0 0.0 1.534855461041929 0.0 78-79 9.290330252659822E-5 0.0 0.0 1.7236349717759767 0.0 80-81 1.8580660505319643E-4 0.0 0.0 1.9283473988933357 0.0 82-83 1.8580660505319643E-4 0.0 0.0 2.1711966316978635 0.0 84-85 1.8580660505319643E-4 0.0 0.0 2.495243350910638 0.0 86-87 1.8580660505319643E-4 0.0 0.0 2.888270772249412 0.0 88-89 2.7870990757979466E-4 0.0 0.0 3.33852662794457 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGG 355 0.0 29.447102 1 GTATCAA 15855 0.0 26.46322 1 GGTATCA 11490 0.0 25.30927 1 GTACATG 11175 0.0 25.04471 1 AGGTAAG 440 0.0 24.83377 2 TACATGG 11285 0.0 24.50129 2 GTAAGGT 515 0.0 23.980207 4 TATAGGG 260 2.3646862E-11 23.754042 2 ATGGGAG 2580 0.0 23.749628 5 ACATGGG 11340 0.0 23.708845 3 AGTACTT 9950 0.0 23.510939 12-13 GAGTACT 9725 0.0 23.248993 12-13 GTATTAC 105 0.0021915918 22.627102 1 TAAGGTG 550 0.0 22.454195 5 ACCGTGC 170 3.1503769E-6 22.351551 8 CATGGGA 7470 0.0 22.128168 4 GTACTTT 10240 0.0 21.98696 14-15 ATGGGAT 2340 0.0 21.922733 5 ATCAACG 19565 0.0 21.535292 3 TCAACGC 19700 0.0 21.459057 4 >>END_MODULE