FastQCFastQC Report
Thu 26 May 2016
SRR936410_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936410_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113322
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112231.0080641539464772No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86610.777942050907105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52320.4699449036307556No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29300.26317633173511346No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20050.1800916536276118No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19310.17344487937901165No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18550.16661846258315205No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16380.1471272462055003No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14380.12916299147955398No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13850.1244024639771782No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13180.11838443864398619No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG11780.10580946033582378No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC11530.10356392849508048No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG651.323811E-436.5588265
GTATCAA148400.029.7200581
GTACATG117200.028.8321861
TACATGG119350.027.715482
GGTATCA102150.026.7525421
ACATGGG119250.026.3438153
GAGTACT82700.024.961112-13
ATCAACG178550.024.4885793
TCAACGC178950.024.3276064
TATCAAC180250.024.2839872
CATGGGA76500.024.1670554
CAACGCA180650.024.0197455
ATGGGAG25950.023.992175
CCCTATA2801.8189894E-1223.7632352
AACGCAG183650.023.6284356
GTACTTT87900.023.29422814-15
AGTACTT85650.023.13066712-13
CATGGGG37600.022.7520374
GTGTAAG4650.022.4856451
TTCGCGA853.1568416E-622.34832448-49
Produced by FastQC (version 0.11.5)