FastQCFastQC Report
Thu 26 May 2016
SRR936410_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936410_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113322
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119201.0706695816664002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98250.8824940134121125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66130.5939880825134148No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33900.30449411760479No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24390.21907408638291528No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23130.20775660590556913No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18050.16212739890166547No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16260.14604939092194352No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15960.14335475271305156No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13770.12368389378814035No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13360.12000122156932136No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG11900.10688731561938056No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11810.10607892415671297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA169250.027.6574861
GGTATCA119650.026.1219161
GTACATG113800.024.3427851
TACATGG115950.023.764232
GAGTACT105250.023.6890512-13
ATCAACG206050.022.7527053
ACATGGG117600.022.7399543
TCAACGC207800.022.5600784
TATCAAC208300.022.5337872
ATGGGAT24000.022.3603025
CAACGCA209900.022.3539855
GTACTTT110950.022.34362614-15
AGTACTT107500.022.17714912-13
AACGCAG213150.022.0131446
ATGGGCG3301.8189894E-1221.5867755
CATGGGG36150.021.153714
ATGGGAG25950.021.0460645
ACTTTTT122200.020.30787816-17
TACTTTT117200.020.19984214-15
ACGCAGA233700.020.0571357
Produced by FastQC (version 0.11.5)