##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936410_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1113322 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.48097675245796 31.0 31.0 34.0 30.0 34.0 2 31.664978326126672 31.0 31.0 34.0 30.0 34.0 3 31.90607119952718 31.0 31.0 34.0 30.0 34.0 4 35.75262323029636 37.0 35.0 37.0 35.0 37.0 5 35.58012147429046 37.0 35.0 37.0 33.0 37.0 6 35.710300344374765 37.0 35.0 37.0 33.0 37.0 7 35.642109829860544 37.0 35.0 37.0 33.0 37.0 8 35.63041599824669 37.0 35.0 37.0 33.0 37.0 9 37.22587984428584 39.0 37.0 39.0 33.0 39.0 10-11 37.11906438568536 39.0 37.0 39.0 33.0 39.0 12-13 36.984691311228914 39.0 37.0 39.0 33.0 39.0 14-15 37.98130370189397 40.0 37.0 41.0 33.0 41.0 16-17 37.92668069076152 40.0 37.0 41.0 33.0 41.0 18-19 38.08757664000173 40.0 37.0 41.0 33.0 41.0 20-21 38.18756568180635 40.0 37.0 41.0 33.0 41.0 22-23 38.093423555808656 40.0 37.0 41.0 33.0 41.0 24-25 37.962697225061575 40.0 37.0 41.0 33.0 41.0 26-27 37.71552030769176 40.0 37.0 41.0 32.5 41.0 28-29 37.58970091312307 39.0 36.5 41.0 32.0 41.0 30-31 37.43550113983196 39.0 36.0 41.0 32.0 41.0 32-33 37.12656446203344 39.0 35.0 41.0 31.5 41.0 34-35 36.89196836135458 39.0 35.0 41.0 31.0 41.0 36-37 36.58046728619394 39.0 35.0 41.0 31.0 41.0 38-39 36.353269763823945 39.0 35.0 40.5 30.0 41.0 40-41 36.025943078462475 38.5 35.0 40.0 30.0 41.0 42-43 35.638048560973374 38.0 35.0 40.0 28.5 41.0 44-45 35.1477137791223 38.0 34.5 40.0 26.5 41.0 46-47 34.460561275174655 38.0 34.0 40.0 23.5 41.0 48-49 33.797673090085354 38.0 33.0 40.0 19.5 41.0 50-51 33.51783670851739 38.0 33.0 40.0 17.5 41.0 52-53 33.21801599177955 37.5 33.0 40.0 14.5 41.0 54-55 32.92202525414929 37.0 33.0 40.0 12.0 41.0 56-57 32.632095206957196 37.0 32.0 40.0 8.5 41.0 58-59 32.25034176994616 36.0 31.5 39.5 7.5 41.0 60-61 31.859224465159226 36.0 31.0 39.0 6.0 40.5 62-63 31.55724085215239 35.0 31.0 39.0 2.0 40.0 64-65 31.107181480290517 35.0 30.5 38.5 2.0 40.0 66-67 30.692671122999457 35.0 30.0 38.0 2.0 40.0 68-69 30.245677351206567 35.0 29.0 37.5 2.0 40.0 70-71 29.784610382261377 34.0 29.0 37.0 2.0 39.0 72-73 29.38096884818588 34.0 29.0 36.0 2.0 39.0 74-75 29.037094838689974 34.0 28.5 36.0 2.0 39.0 76-77 27.64604984002831 32.5 26.5 34.5 2.0 36.5 78-79 28.425075584601757 33.5 28.0 35.0 2.0 37.0 80-81 28.274601148634446 34.0 28.0 35.0 2.0 37.0 82-83 27.941847012813902 34.0 27.0 35.0 2.0 36.0 84-85 27.50047964560118 33.0 26.0 35.0 2.0 36.0 86-87 27.123108139424176 33.0 26.0 35.0 2.0 35.5 88-89 26.704778581578374 33.0 25.0 35.0 2.0 35.0 90-91 26.242709207219477 33.0 24.5 35.0 2.0 35.0 92-93 25.801824180246147 32.0 23.5 34.5 2.0 35.0 94-95 25.287654425224687 32.0 20.0 34.0 2.0 35.0 96-97 24.63059968275126 32.0 14.5 34.0 2.0 35.0 98-99 23.81971882348503 31.0 2.0 34.0 2.0 35.0 100-101 21.218574680101533 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.40161855597159146 1101 2 0.4550468563688739 1101 3 0.3103060969092901 1101 4 0.24386918622413845 1101 5 0.28765579756022674 1101 6 0.23005350721776097 1101 7 0.2950858039369493 1101 8 0.2905781576315647 1101 9 0.3765650746504505 1101 10-11 0.3285019903112385 1101 12-13 0.3286787213445521 1101 14-15 0.512846562178467 1101 16-17 0.37896356143084375 1101 18-19 0.3597562040121929 1101 20-21 0.2095811062972075 1101 22-23 0.24651834024133734 1101 24-25 -0.04491042398092304 1101 26-27 0.1564645551417243 1101 28-29 0.1874776626859287 1101 30-31 0.25837472992897403 1101 32-33 0.45973285816080534 1101 34-35 0.6123281914463448 1101 36-37 0.6175052288636635 1101 38-39 0.8041901423546349 1101 40-41 0.9139864823474468 1101 42-43 0.8174629869059586 1101 44-45 1.0446745952507257 1101 46-47 0.8676733877054374 1101 48-49 0.8886310380199234 1101 50-51 0.745621131499064 1101 52-53 0.7069361702275501 1101 54-55 0.8163373634045641 1101 56-57 0.9265487260904237 1101 58-59 0.9050744441459599 1101 60-61 0.8502242647364149 1101 62-63 0.6708944534571657 1101 64-65 0.8203822069470981 1101 66-67 0.4291411818945434 1101 68-69 0.2740616981072215 1101 70-71 0.2890072861661288 1101 72-73 0.4374362193463348 1101 74-75 0.3013851995146837 1101 76-77 0.8000255868126445 1101 78-79 0.5599254315104218 1101 80-81 0.44611314177246797 1101 82-83 0.6270892829560708 1101 84-85 0.7496500044236036 1101 86-87 0.6739111648730685 1101 88-89 0.5840311832164353 1101 90-91 0.4384135176255235 1101 92-93 0.5962844944766807 1101 94-95 0.3391941617618208 1101 96-97 0.5161932271855925 1101 98-99 0.6459769270898157 1101 100-101 0.566426932593636 1102 1 0.5203158000647683 1102 2 0.5320473670310335 1102 3 0.17092536656226898 1102 4 0.05544018995897915 1102 5 0.17355317782997304 1102 6 0.0898363589822111 1102 7 0.0884572088586637 1102 8 -0.0769784407576779 1102 9 0.10708765225941619 1102 10-11 0.2058489502025651 1102 12-13 0.2900896842264231 1102 14-15 0.26807517528012426 1102 16-17 0.2728439596805643 1102 18-19 0.11713193454277615 1102 20-21 0.1541912841611719 1102 22-23 0.1793332235400129 1102 24-25 0.12572182917399743 1102 26-27 -0.03452442723875748 1102 28-29 -0.1306555166699752 1102 30-31 -0.04672521633467852 1102 32-33 0.13991249108483572 1102 34-35 0.20071947214031383 1102 36-37 0.11644652274139133 1102 38-39 0.004307294261607808 1102 40-41 0.2221393872808477 1102 42-43 -0.09843128933889744 1102 44-45 -0.21941717708072872 1102 46-47 -0.07539545823146199 1102 48-49 -0.1075253113797956 1102 50-51 -0.07251838941305522 1102 52-53 -0.062163428919333796 1102 54-55 0.17439831577279818 1102 56-57 0.030453455890068426 1102 58-59 -0.021630850385953693 1102 60-61 0.10741475507221665 1102 62-63 -0.11508703587400504 1102 64-65 -0.14930882575864857 1102 66-67 -0.004957365889083576 1102 68-69 0.02688519257170796 1102 70-71 -0.22155274000113323 1102 72-73 -0.4115890167097511 1102 74-75 -0.6868221760489632 1102 76-77 -0.41363145515176925 1102 78-79 -0.07691767505108871 1102 80-81 -0.19654216794580037 1102 82-83 -0.3162862940421043 1102 84-85 -0.20816763244553727 1102 86-87 -0.4141586990504855 1102 88-89 -0.3948160553695992 1102 90-91 -0.40361130789824173 1102 92-93 -0.4318843644770958 1102 94-95 -0.6159433331899642 1102 96-97 -0.6341510525638192 1102 98-99 -0.8872758573540125 1102 100-101 -0.9290361845816335 1103 1 0.45329642815967475 1103 2 0.5549387972615278 1103 3 0.24266109571045646 1103 4 0.1781751610059672 1103 5 0.08995558439803375 1103 6 0.13005120808781356 1103 7 0.049239182179952934 1103 8 0.08894249090030115 1103 9 0.005692423458022233 1103 10-11 0.20253266070468356 1103 12-13 0.33524832093464596 1103 14-15 0.2330460609681353 1103 16-17 0.07492793148565369 1103 18-19 0.2689586358347711 1103 20-21 0.24699254873849696 1103 22-23 0.1685794773910061 1103 24-25 0.20190938663645852 1103 26-27 0.19867395332803994 1103 28-29 0.24265448220094044 1103 30-31 0.16219457010659255 1103 32-33 0.05509387082901185 1103 34-35 0.23727769269937227 1103 36-37 0.4372475292417022 1103 38-39 0.4046637631230112 1103 40-41 0.3605960867985658 1103 42-43 0.19687493515582588 1103 44-45 0.21524901644475847 1103 46-47 0.2507961316841829 1103 48-49 0.3196114855619854 1103 50-51 -0.1047554331409728 1103 52-53 -0.09046318535916953 1103 54-55 -0.03152132550015807 1103 56-57 -0.18954912487996722 1103 58-59 -0.13484600595798923 1103 60-61 -0.048418591702624525 1103 62-63 -0.3051236505489072 1103 64-65 -0.15835281175796823 1103 66-67 -0.17323808914988348 1103 68-69 -0.29526265329180035 1103 70-71 -0.20935376263126315 1103 72-73 -0.3406130017412856 1103 74-75 -0.2254643684131068 1103 76-77 -0.44725404979344674 1103 78-79 -0.3672043018233495 1103 80-81 -0.3088137294729698 1103 82-83 0.06374661077586907 1103 84-85 0.22429684164191954 1103 86-87 0.06278534662914481 1103 88-89 0.14122762416328527 1103 90-91 -0.18508621797453273 1103 92-93 -0.11990959150421077 1103 94-95 -0.17713984269849092 1103 96-97 -0.3950475475555102 1103 98-99 -0.2311263662496046 1103 100-101 0.2749140065566493 1104 1 0.2374165847016485 1104 2 0.30653771133972896 1104 3 0.18831179941214415 1104 4 0.08167748317345058 1104 5 0.011070481206616023 1104 6 0.07357558203209891 1104 7 0.010671650216096396 1104 8 -0.11584223183385944 1104 9 -0.045366414400518806 1104 10-11 -0.19999560114734294 1104 12-13 -0.23600071349881802 1104 14-15 0.117403722838759 1104 16-17 0.024369425365328823 1104 18-19 0.052061141339706296 1104 20-21 -0.04622204261452367 1104 22-23 -0.029836833231350113 1104 24-25 0.06936314361176699 1104 26-27 0.06898771036745899 1104 28-29 -0.04585936266096269 1104 30-31 0.023972879235330424 1104 32-33 -0.005283765848730582 1104 34-35 -0.03511944441588355 1104 36-37 -0.06823400399782287 1104 38-39 -0.18782549144360416 1104 40-41 -0.015507223211592702 1104 42-43 0.16837782143853275 1104 44-45 0.0646555736431651 1104 46-47 0.07283316456632605 1104 48-49 -0.03920457761891072 1104 50-51 0.0519522494031861 1104 52-53 0.03469378931654887 1104 54-55 0.265330206425233 1104 56-57 0.07153045062332808 1104 58-59 0.11108515275390118 1104 60-61 0.0789929207351534 1104 62-63 0.05538892505374804 1104 64-65 -0.1525590920270794 1104 66-67 0.09562435327670826 1104 68-69 0.044891452729810766 1104 70-71 0.14170058794711693 1104 72-73 0.13163701668861805 1104 74-75 -0.1488482263842812 1104 76-77 0.03900044950621506 1104 78-79 0.12121274599503096 1104 80-81 0.09182442587025719 1104 82-83 0.06261677773474617 1104 84-85 0.18981882450691856 1104 86-87 0.17829979727386913 1104 88-89 -0.049125044005553065 1104 90-91 -0.18438395862897394 1104 92-93 -0.05802166453149127 1104 94-95 0.05330795580981018 1104 96-97 -0.20476250910913407 1104 98-99 -0.15011813850090405 1104 100-101 0.10972728762385131 1105 1 0.3920795123832903 1105 2 0.3676884356506456 1105 3 0.2680430075535334 1105 4 0.09623159767965461 1105 5 -0.014074790915998392 1105 6 -0.024261477475661763 1105 7 0.07736679910080824 1105 8 0.14411416090817397 1105 9 -0.026322827639496893 1105 10-11 0.041860064465438995 1105 12-13 0.05078458352759441 1105 14-15 0.1588517553879143 1105 16-17 0.1688998146536207 1105 18-19 0.1178243195965436 1105 20-21 0.05000248352164505 1105 22-23 0.16719488835859408 1105 24-25 -0.02471913113957669 1105 26-27 -0.12688337151272577 1105 28-29 0.019069205473449813 1105 30-31 -0.018616897399176935 1105 32-33 -0.08396866882129927 1105 34-35 -0.057799958479350266 1105 36-37 -0.055997325733301295 1105 38-39 -0.14171600443098242 1105 40-41 -0.13532373709058731 1105 42-43 0.026620284966774932 1105 44-45 -0.028667673483425915 1105 46-47 0.18174145865654623 1105 48-49 0.40749022537978874 1105 50-51 0.4171867344942157 1105 52-53 0.19403322390822098 1105 54-55 0.15365738640381466 1105 56-57 0.07441571639188993 1105 58-59 0.14410221390142652 1105 60-61 0.11056014303992967 1105 62-63 0.12500392830764184 1105 64-65 0.24058551138259077 1105 66-67 0.2160801157375687 1105 68-69 0.1808976735536838 1105 70-71 -0.11148092278047272 1105 72-73 -0.07711159336206208 1105 74-75 0.08439936138109161 1105 76-77 -0.11142697592331885 1105 78-79 0.08936401091296631 1105 80-81 0.20475492584207444 1105 82-83 0.2580077826488889 1105 84-85 0.5437870337397719 1105 86-87 0.21511519919340927 1105 88-89 0.13818279710210035 1105 90-91 0.11703872175698748 1105 92-93 0.4253447414823164 1105 94-95 0.5942941664876962 1105 96-97 0.3297053516755888 1105 98-99 0.5685887858807313 1105 100-101 0.9079306660333906 1106 1 0.20869699378632944 1106 2 0.2943199848177436 1106 3 0.08811254176000105 1106 4 -0.02411397097466761 1106 5 -0.076678287611891 1106 6 -0.10884974880182341 1106 7 0.0050728970352835745 1106 8 0.042261732272223185 1106 9 0.12298040275517508 1106 10-11 -3.737251617863535E-4 1106 12-13 -0.04452289574711443 1106 14-15 0.034810803140445046 1106 16-17 -0.121733182818339 1106 18-19 -0.07815494732241035 1106 20-21 -0.007900186757986205 1106 22-23 -0.06761186157261534 1106 24-25 -0.1081381169108937 1106 26-27 -0.03832686166788335 1106 28-29 -0.08452806714527838 1106 30-31 0.08540377079366124 1106 32-33 0.1329678679132087 1106 34-35 0.06432808931989342 1106 36-37 -0.016350629062330313 1106 38-39 -0.08385810701665264 1106 40-41 0.019934608831057687 1106 42-43 0.006178551984362457 1106 44-45 0.09427277471242235 1106 46-47 0.1391537038405417 1106 48-49 0.07754178555192226 1106 50-51 0.04588666026210575 1106 52-53 0.2737016711073892 1106 54-55 0.1108055365707088 1106 56-57 0.1750673886908487 1106 58-59 0.03707657267612774 1106 60-61 0.3501436385937886 1106 62-63 0.08222215774197394 1106 64-65 0.09237013183846798 1106 66-67 -0.00212368774977989 1106 68-69 0.1940557048972842 1106 70-71 0.08079128066742314 1106 72-73 0.09963351017356459 1106 74-75 -0.0993744331257318 1106 76-77 -0.025792382201384356 1106 78-79 0.03899421300080874 1106 80-81 0.03170585478169485 1106 82-83 0.3194296650299222 1106 84-85 0.22484201222284028 1106 86-87 0.17595873295345044 1106 88-89 0.12743828275142377 1106 90-91 0.0780516137466627 1106 92-93 0.0076336990661864945 1106 94-95 -0.07265022172029845 1106 96-97 0.21773977097801733 1106 98-99 -0.054583962109035156 1106 100-101 0.3633982115459844 1107 1 0.5927312539124223 1107 2 0.5348414913180193 1107 3 0.3518962739565801 1107 4 0.131594939142488 1107 5 -0.0015638397640529433 1107 6 0.1850653522546395 1107 7 0.15726581172126686 1107 8 0.1261957085131229 1107 9 0.30290135723962663 1107 10-11 0.33687817826270816 1107 12-13 0.2513963681509921 1107 14-15 0.33263765740765905 1107 16-17 0.39636411981782516 1107 18-19 0.3062462881992971 1107 20-21 0.23360446538417534 1107 22-23 0.35193342220653534 1107 24-25 0.25351708224805947 1107 26-27 0.1746033213577789 1107 28-29 0.43859895708948926 1107 30-31 0.4212627583202817 1107 32-33 0.3432875644559914 1107 34-35 0.32746156686813066 1107 36-37 0.4082338442049007 1107 38-39 0.3756534104815259 1107 40-41 0.41234189168311985 1107 42-43 0.4643763051405756 1107 44-45 0.44185300939489736 1107 46-47 0.3085543182686976 1107 48-49 0.5140819309181168 1107 50-51 0.36585246981967146 1107 52-53 0.26490789537366766 1107 54-55 0.43862855823626745 1107 56-57 0.5514996910088925 1107 58-59 0.4820177657210891 1107 60-61 0.47599120405856254 1107 62-63 0.45416743788545944 1107 64-65 0.1577163687689911 1107 66-67 0.42613356919813583 1107 68-69 0.4447286824803527 1107 70-71 0.04281784832269153 1107 72-73 0.15340127572135742 1107 74-75 0.4420047884168241 1107 76-77 0.42961764558118887 1107 78-79 0.5356634094875403 1107 80-81 0.49596381462572836 1107 82-83 0.37641045354605396 1107 84-85 0.3125283024159238 1107 86-87 0.3430764498741574 1107 88-89 0.3843040174747969 1107 90-91 0.3958269323979522 1107 92-93 0.6038263198688831 1107 94-95 0.5980649144809718 1107 96-97 0.3783299461869909 1107 98-99 0.7399151183695416 1107 100-101 0.6175371941038605 1108 1 0.2879141860842047 1108 2 0.32808057359055454 1108 3 0.12150061976910465 1108 4 0.05503942113715965 1108 5 -0.01495675278521702 1108 6 0.012813089853679571 1108 7 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2316 90-91 -0.9331018058960723 2316 92-93 -0.8908498740897137 2316 94-95 -1.2849860534837632 2316 96-97 -1.4748050203963885 2316 98-99 -2.1703160555797147 2316 100-101 -3.170369340927728 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 9.0 4 35.0 5 62.0 6 144.0 7 342.0 8 715.0 9 1352.0 10 2265.0 11 3815.0 12 5441.0 13 7540.0 14 9564.0 15 13162.0 16 17764.0 17 22457.0 18 17628.0 19 9880.0 20 8345.0 21 8281.0 22 8820.0 23 9758.0 24 10989.0 25 12957.0 26 15511.0 27 19005.0 28 23322.0 29 28302.0 30 34039.0 31 41552.0 32 51734.0 33 66043.0 34 86432.0 35 113774.0 36 153502.0 37 188930.0 38 112837.0 39 7014.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.32631275166689 19.48529044752578 15.38538328691364 19.803013513893692 2 15.86889068953932 22.065563638519624 39.04200406404007 23.02354160790098 3 19.54002132458552 28.021439562226202 30.322859973645183 22.115679139543094 4 12.70191192548135 17.5543559794497 36.86738156132346 32.876350533745494 5 12.45066566545887 39.87489693008851 32.17856109912496 15.495876305327657 6 29.397065718633066 37.12241382097902 17.179576079516977 16.300944380870945 7 26.974855432660092 36.0320733803877 19.82508205173346 17.167989135218743 8 28.055315533152136 32.35766471874265 19.2188782760064 20.36814147209882 9 28.936795160268936 15.668764063110524 20.028833587086865 35.36560718953368 10-11 25.27773922939462 27.44081999821255 25.17386069317927 22.10758007921356 12-13 27.961065983304884 25.405779742472852 26.589402233590626 20.04375204063164 14-15 24.26900046392709 25.51325021568343 27.59086211844467 22.62688720194481 16-17 20.955797154821337 28.99394784258283 27.97730575700471 22.072949245591122 18-19 20.910617054185582 25.82698446630894 33.05584547866655 20.20655300083893 20-21 22.614481704304772 24.2356658720478 33.13794212276412 20.0119103008833 22-23 21.923721745738252 24.58098877621641 33.10852232383654 20.386767154208798 24-25 21.544351050280152 24.908606854081746 33.283093300949766 20.26394879468833 26-27 21.00651069794305 24.954876017394795 33.58661446850707 20.451998816155083 28-29 20.762367042059708 25.44739976394969 33.44948721034885 20.34074598364175 30-31 21.02478933533575 25.161060843610763 33.1795891842563 20.634560636797186 32-33 20.685493831319025 25.40674670008129 32.98809379449108 20.919665674108607 34-35 21.38821909453584 25.246548943541313 32.87136610053637 20.493865861386475 36-37 21.60080194594051 25.08928475188899 32.64475986533632 20.66515343683418 38-39 21.383644691712764 25.29653410675542 32.70563148706954 20.614189714462277 40-41 21.259733598734552 25.48242450482043 32.46187177923313 20.795970117211887 42-43 21.075930860799485 25.88727038677115 31.59408859206785 21.442710160361514 44-45 21.29895980278425 25.731643400220154 31.42553412225023 21.543862674745363 46-47 21.943158484729267 26.298205524806296 30.372826518495366 21.385809471969072 48-49 22.044154873528814 26.649811627192516 29.42738978349254 21.878643715786126 50-51 21.96403990775318 27.10201716940585 29.32092597125259 21.613016951588385 52-53 22.26840617748202 27.131733856462066 28.699188854052565 21.90067111200335 54-55 21.57193354383475 27.014413208589538 28.91305812444137 22.500595123134346 56-57 22.242687265248964 27.28041136083853 28.056990129621422 22.419911244291082 58-59 22.430534877736267 26.74285385793092 28.410313205525323 22.41629805880749 60-61 22.184016301592028 26.977796401666986 29.07320850237432 21.764978794366662 62-63 22.41281432876577 26.94795133321656 29.017457344705083 21.621776993312583 64-65 22.184630560104786 27.181077519177887 28.903246787664706 21.731045133052625 66-67 22.15434018616036 27.21736299024286 28.438745607042204 22.189551216554573 68-69 22.383935953637707 27.177083646773138 28.588853953293693 21.850126446295466 70-71 22.37890187117358 27.318891739242908 28.21814549739875 22.08406089218476 72-73 22.15514566097933 27.507687581709327 28.156287289879877 22.180879467431467 74-75 22.00432579253801 28.18780191175599 27.748081866701636 22.059790429004366 76-77 21.876966260596195 27.533913434008873 28.285184977263793 22.303935328131143 78-79 21.76713025262171 27.412356742597506 28.380326731979526 22.44018627280126 80-81 21.85910338189075 28.04753539394304 27.87927407774068 22.214087146425527 82-83 21.97382309478573 27.706691100840374 27.682436590175126 22.637049214198772 84-85 21.634361447168434 27.74330869644891 27.565628492071763 23.056701364310893 86-87 21.64606868747844 27.88241455386915 27.32281965045418 23.14869710819823 88-89 21.6986001555233 27.537462382633144 27.341867798006586 23.422069663836968 90-91 21.637517794565476 27.41394033783732 27.56833584819377 23.38020601940343 92-93 21.659484549278158 27.464895950992435 27.701265834154842 23.174353665574564 94-95 21.288811436938293 27.300839445400115 27.791346840514358 23.61900227714723 96-97 21.177890434917746 27.01169275712006 27.98810287901524 23.82231392894695 98-99 21.04787061311808 27.19499613508387 27.546633884871763 24.21049936692629 100-101 21.03687800233112 27.360733382890277 27.572645464978134 24.02974314980046 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1196.0 1 897.0 2 814.0 3 1013.0 4 871.0 5 805.5 6 1285.5 7 2555.5 8 3848.5 9 4277.5 10 3709.0 11 2991.0 12 2820.5 13 2888.5 14 3007.5 15 3109.0 16 3219.0 17 3350.5 18 3499.0 19 3673.5 20 3893.5 21 4223.0 22 4659.0 23 5203.5 24 5791.5 25 6491.0 26 7482.5 27 8740.0 28 10212.5 29 12090.5 30 14304.5 31 16293.0 32 19056.5 33 21073.5 34 22229.0 35 23602.5 36 26066.0 37 28499.0 38 29445.5 39 29812.0 40 29243.0 41 29306.0 42 29553.0 43 30573.5 44 32064.5 45 33596.0 46 34572.0 47 37517.0 48 46807.5 49 46529.0 50 38180.5 51 34067.0 52 31667.0 53 32215.0 54 32733.0 55 31540.0 56 29361.5 57 27282.0 58 26673.0 59 24872.0 60 21559.0 61 19128.5 62 16744.5 63 12395.0 64 8373.5 65 6560.0 66 4810.0 67 3668.5 68 2949.5 69 2078.5 70 1504.0 71 1209.5 72 1061.0 73 1077.5 74 938.0 75 773.0 76 602.0 77 379.0 78 232.5 79 149.0 80 117.5 81 90.0 82 62.0 83 41.5 84 26.0 85 16.0 86 7.5 87 5.5 88 4.5 89 1.5 90 0.5 91 0.5 92 0.5 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0256691235779047 2 0.012934263402681346 3 0.004760527502375773 4 0.013563012318089466 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0033233871243000673 10-11 2.245531840743289E-4 12-13 2.245531840743289E-4 14-15 4.491063681486578E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014820510148905708 24-25 0.0 26-27 4.491063681486578E-5 28-29 0.0 30-31 4.491063681486578E-5 32-33 0.0033233871243000673 34-35 3.1437445770406044E-4 36-37 0.0026946382088919468 38-39 1.3473191044459734E-4 40-41 0.0035030296715595306 42-43 0.01064382092512319 44-45 0.002110799930298692 46-47 0.001437140378075705 48-49 0.009341412457492083 50-51 4.0419573133379207E-4 52-53 0.0018413361094094971 54-55 0.009610876278381277 56-57 0.0037275828556338595 58-59 0.001032944646741913 60-61 0.005074901960079833 62-63 0.0033233871243000673 64-65 0.01715586326327873 66-67 0.0030988339402257385 68-69 3.1437445770406044E-4 70-71 8.982127362973156E-4 72-73 4.491063681486578E-5 74-75 0.0 76-77 8.533020994824498E-4 78-79 0.01041926774104886 80-81 0.015494169701128695 82-83 0.011407301750975908 84-85 0.007724629532156914 86-87 0.009521055004751545 88-89 0.02744039909388299 90-91 0.02438647579047212 92-93 0.002200621203928423 94-95 0.007814450805786645 96-97 0.010015072009715069 98-99 0.009206680547047486 100-101 0.01118274856690158 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1113322.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.48649342942145 #Duplication Level Percentage of deduplicated Percentage of total 1 71.00730033430584 32.29873100097359 2 14.712108695891668 13.384044710572212 3 5.6947610598275995 7.771041345899198 4 2.769358467476498 5.0387362293832965 5 1.5818013323720688 3.5975297955796104 6 0.9443730897307813 2.577373220457697 7 0.6538659259320595 2.081944770054184 8 0.4373689251475988 1.5915502991967498 9 0.3156514202617823 1.29220886283526 >10 1.680371930597326 13.768328158795375 >50 0.12385817323920828 3.8745781537496 >100 0.06965515468886123 6.288354188054695 >500 0.00674722245781046 2.137791201626135 >1k 0.0021829249128210316 1.7379990624544865 >5k 3.96895438694733E-4 1.4838074472123635 >10k+ 1.984477193473665E-4 1.075981553155577 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11920 1.0706695816664002 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9825 0.8824940134121125 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6613 0.5939880825134148 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3390 0.30449411760479 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2439 0.21907408638291528 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2313 0.20775660590556913 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1805 0.16212739890166547 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1626 0.14604939092194352 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1596 0.14335475271305156 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1377 0.12368389378814035 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1336 0.12000122156932136 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1190 0.10688731561938056 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1181 0.10607892415671297 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 1.7964254725946313E-4 0.0 28-29 0.0 0.0 0.0 5.838382785932552E-4 0.0 30-31 0.0 0.0 0.0 0.0017964254725946313 0.0 32-33 0.0 0.0 0.0 0.0030090126665960073 0.0 34-35 0.0 0.0 0.0 0.003907225402893323 0.0 36-37 0.0 0.0 0.0 0.005658740238673088 0.0 38-39 0.0 0.0 0.0 0.007589897621712317 0.0 40-41 0.0 0.0 0.0 0.01149712302460564 0.0 42-43 0.0 0.0 0.0 0.016886399442389535 0.0 44-45 0.0 0.0 0.0 0.030135037302774937 0.0 46-47 0.0 0.0 0.0 0.05393767481465381 0.0 48-49 0.0 0.0 0.0 0.07275523164008257 0.0 50-51 0.0 0.0 0.0 0.09884831162951958 0.0 52-53 0.0 0.0 0.0 0.1323067360565946 0.0 54-55 0.0 0.0 0.0 0.17227720282182513 0.0 56-57 0.0 0.0 0.0 0.213145882323353 0.0 58-59 0.0 0.0 0.0 0.27781719933675975 0.0 60-61 0.0 0.0 0.0 0.3560964393050708 0.0 62-63 0.0 0.0 0.0 0.45849269124296477 0.0 64-65 0.0 0.0 0.0 0.5355144333804596 0.0 66-67 0.0 0.0 0.0 0.6501712891688118 0.0 68-69 0.0 0.0 0.0 0.7949631822599392 0.0 70-71 0.0 0.0 0.0 0.9113266422472563 0.0 72-73 0.0 0.0 0.0 1.0830200067904885 0.0 74-75 0.0 0.0 0.0 1.2248028872150196 0.0 76-77 0.0 0.0 0.0 1.3575138190029479 0.0 78-79 0.0 0.0 0.0 1.53751565135693 0.0 80-81 1.7964254725946313E-4 0.0 0.0 1.7148228455020202 0.0 82-83 1.7964254725946313E-4 0.0 0.0 1.9405886167703503 0.0 84-85 1.7964254725946313E-4 0.0 0.0 2.244184521638843 0.0 86-87 1.7964254725946313E-4 0.0 0.0 2.619637445411121 0.0 88-89 1.7964254725946313E-4 0.0 0.0 3.0534741970427244 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16925 0.0 27.657486 1 GGTATCA 11965 0.0 26.121916 1 GTACATG 11380 0.0 24.342785 1 TACATGG 11595 0.0 23.76423 2 GAGTACT 10525 0.0 23.68905 12-13 ATCAACG 20605 0.0 22.752705 3 ACATGGG 11760 0.0 22.739954 3 TCAACGC 20780 0.0 22.560078 4 TATCAAC 20830 0.0 22.533787 2 ATGGGAT 2400 0.0 22.360302 5 CAACGCA 20990 0.0 22.353985 5 GTACTTT 11095 0.0 22.343626 14-15 AGTACTT 10750 0.0 22.177149 12-13 AACGCAG 21315 0.0 22.013144 6 ATGGGCG 330 1.8189894E-12 21.586775 5 CATGGGG 3615 0.0 21.15371 4 ATGGGAG 2595 0.0 21.046064 5 ACTTTTT 12220 0.0 20.307878 16-17 TACTTTT 11720 0.0 20.199842 14-15 ACGCAGA 23370 0.0 20.057135 7 >>END_MODULE