FastQCFastQC Report
Thu 26 May 2016
SRR936409_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936409_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121477
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112491.0030522248784415No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86820.7741576510262805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53230.47464192310676007No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29440.2625109565332147No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20380.18172463635009903No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19810.17664205329222088No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19010.169508603386427No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17090.1523883236125217No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13520.12055530340791654No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13490.12028779903644925No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13190.11761275532177655No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG12440.11092514603509478No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC11420.10182999740520761No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGAG650.005829009729.246153
GTACATG120350.028.7861841
GTATCAA152800.028.675451
TACATGG121550.027.9949762
GGTATCA106450.027.2324581
TCGACCG350.00838176327.15047318-19
ACATGGG120700.026.499023
ATGGGAG26350.024.889755
CATGGGA79400.024.7811374
GAGTACT88100.024.35805712-13
CATGCGT1000.001648137323.7635564
ATCAACG184250.023.7302553
TCAACGC185450.023.5777534
ATGGGAT25950.023.4425
CAACGCA186650.023.4251235
TATCAAC187200.023.3816872
TATAGGG2653.092282E-1123.3141462
AGTACTT89750.023.22180412-13
GTACTTT92050.023.07840314-15
AACGCAG190750.022.9465376
Produced by FastQC (version 0.11.5)