##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936409_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1121477 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.56895861439869 31.0 31.0 34.0 30.0 34.0 2 31.573446446070673 33.0 31.0 34.0 30.0 34.0 3 31.595478997785957 33.0 31.0 34.0 28.0 34.0 4 35.26744462882431 37.0 35.0 37.0 33.0 37.0 5 35.259672735151945 37.0 35.0 37.0 33.0 37.0 6 35.422125464900304 37.0 35.0 37.0 33.0 37.0 7 35.29906186216927 37.0 35.0 37.0 33.0 37.0 8 35.2494612016118 37.0 35.0 37.0 32.0 37.0 9 36.723851670609385 39.0 37.0 39.0 32.0 39.0 10-11 36.64907840285623 39.0 37.0 39.0 32.0 39.0 12-13 36.53389637058986 39.0 37.0 39.0 32.0 39.0 14-15 37.52475931294177 40.0 37.0 41.0 32.0 41.0 16-17 37.44897666202695 40.0 37.0 41.0 32.0 41.0 18-19 37.50384805038355 40.0 37.0 41.0 32.0 41.0 20-21 37.47201770522267 40.0 37.0 41.0 32.0 41.0 22-23 37.34550953786837 39.5 37.0 41.0 32.0 41.0 24-25 37.426458144036836 40.0 37.0 41.0 32.0 41.0 26-27 37.367598711342275 40.0 37.0 41.0 32.0 41.0 28-29 37.264814169171544 39.5 36.0 41.0 31.5 41.0 30-31 36.99708687739472 39.0 36.0 41.0 31.0 41.0 32-33 36.70454989268616 39.0 35.0 41.0 30.5 41.0 34-35 36.43448372102148 39.0 35.0 41.0 30.0 41.0 36-37 36.20330287647451 38.5 35.0 40.0 30.0 41.0 38-39 35.951758707490214 38.0 35.0 40.0 30.0 41.0 40-41 35.695508244930565 38.0 35.0 40.0 29.0 41.0 42-43 35.35298138080407 38.0 35.0 40.0 27.5 41.0 44-45 34.90868738279964 38.0 34.5 40.0 25.5 41.0 46-47 34.340967313640846 38.0 34.0 40.0 23.5 41.0 48-49 34.01082010598523 38.0 33.5 40.0 20.5 41.0 50-51 33.02116450002987 37.0 32.0 39.5 17.5 40.5 52-53 33.12595220410227 37.5 33.0 39.5 18.0 40.5 54-55 33.50536613769164 38.0 33.0 40.0 17.5 41.0 56-57 33.39413871171678 38.0 33.0 40.0 14.5 41.0 58-59 33.12624155466408 37.0 33.0 40.0 12.5 41.0 60-61 32.8016521961663 37.0 33.0 40.0 9.0 41.0 62-63 32.46758827867178 36.0 32.0 39.5 7.5 41.0 64-65 32.11699303686121 36.0 32.0 39.0 6.0 41.0 66-67 31.725592678227017 35.0 31.0 39.0 2.0 41.0 68-69 31.332851676851153 35.0 31.0 38.5 2.0 40.0 70-71 30.905574523597007 35.0 31.0 37.5 2.0 40.0 72-73 30.52742276480035 35.0 30.5 37.0 2.0 39.5 74-75 30.123742617993948 35.0 30.0 36.5 2.0 39.0 76-77 29.713136337169644 34.5 29.5 36.0 2.0 39.0 78-79 29.291476329875692 34.0 29.0 35.5 2.0 37.5 80-81 28.862080987840145 34.0 29.0 35.0 2.0 37.0 82-83 28.39616371980879 34.0 28.0 35.0 2.0 37.0 84-85 28.08963224390692 34.0 27.5 35.0 2.0 36.0 86-87 27.778372628239367 34.0 27.0 35.0 2.0 36.0 88-89 27.38833386685594 34.0 26.0 35.0 2.0 35.5 90-91 26.98797746186502 33.0 25.5 35.0 2.0 35.0 92-93 26.654711153238097 33.0 25.0 35.0 2.0 35.0 94-95 26.21778779234884 33.0 24.0 35.0 2.0 35.0 96-97 25.668072550752267 33.0 21.5 35.0 2.0 35.0 98-99 25.02840628920611 33.0 10.0 35.0 2.0 35.0 100-101 22.745812441984988 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39609122046963563 1101 2 0.5629614565293792 1101 3 0.5787161866561981 1101 4 0.6074259503558253 1101 5 0.5200501375101894 1101 6 0.3865232480796408 1101 7 0.4734394209291395 1101 8 0.7087234086340573 1101 9 0.9211018524940755 1101 10-11 0.6642064505804726 1101 12-13 0.6225216585155451 1101 14-15 0.9397669732676945 1101 16-17 0.9608538218512948 1101 18-19 0.9037041968505619 1101 20-21 0.9589649222455208 1101 22-23 0.8366560787780912 1101 24-25 0.8751922259677158 1101 26-27 0.8543499967746087 1101 28-29 0.9200119138321483 1101 30-31 1.0592372706130817 1101 32-33 0.776405655843206 1101 34-35 0.6914670273547969 1101 36-37 0.7591395841371664 1101 38-39 0.5521954649192935 1101 40-41 0.7030512757871463 1101 42-43 0.9314722727002476 1101 44-45 0.74403504514855 1101 46-47 0.638551859070823 1101 48-49 0.6119727641289003 1101 50-51 0.6114241542925072 1101 52-53 0.7771887446637677 1101 54-55 0.8085616550174066 1101 56-57 0.7147304063339064 1101 58-59 0.7306180548255341 1101 60-61 0.8795865356401436 1101 62-63 0.8703655515413189 1101 64-65 0.9027636447065319 1101 66-67 0.7965984059544979 1101 68-69 0.7665527761249784 1101 70-71 0.5692972886020016 1101 72-73 0.4973538648411555 1101 74-75 0.514107288740572 1101 76-77 0.5311333683669233 1101 78-79 0.6945010603093564 1101 80-81 0.6432357035069032 1101 82-83 0.6978579425670404 1101 84-85 0.7196606025359742 1101 86-87 0.4851144932074156 1101 88-89 0.6094376772955314 1101 90-91 0.42247234450431037 1101 92-93 0.45390006322831056 1101 94-95 0.4852749900352613 1101 96-97 0.38557019103044254 1101 98-99 0.3681053344220615 1101 100-101 0.9169366507096157 1102 1 0.23157893431221055 1102 2 0.3270993368819717 1102 3 0.3938062619370335 1102 4 0.34169710318791147 1102 5 0.2973914952985126 1102 6 0.2492714176297781 1102 7 0.19393981480301647 1102 8 0.2656920108910654 1102 9 0.3701380540801438 1102 10-11 0.3657519059463752 1102 12-13 0.3068875086835945 1102 14-15 0.2796897971951111 1102 16-17 0.4344284947941546 1102 18-19 0.4253127880713734 1102 20-21 0.402366664573357 1102 22-23 0.32069258334406214 1102 24-25 0.18764218593569382 1102 26-27 0.13362881435363505 1102 28-29 0.22872168358031075 1102 30-31 0.0936242004468042 1102 32-33 0.05849960797753795 1102 34-35 -0.04083733465333239 1102 36-37 0.08886939052656118 1102 38-39 0.025439438520081126 1102 40-41 0.13367435718301834 1102 42-43 0.1076644341588775 1102 44-45 -0.005315159652973023 1102 46-47 0.20725932101461808 1102 48-49 0.27762283652175057 1102 50-51 0.3393617378537286 1102 52-53 0.19814773407752995 1102 54-55 0.18720079821603264 1102 56-57 0.18383225786343615 1102 58-59 0.43087101081351875 1102 60-61 0.2658050112257655 1102 62-63 0.33017494494976063 1102 64-65 0.23462236840045847 1102 66-67 0.15381042355569718 1102 68-69 -0.022871033454084255 1102 70-71 0.02196828207955548 1102 72-73 0.031349499351986054 1102 74-75 -0.036580282250195495 1102 76-77 0.0719982664045169 1102 78-79 -0.105839373067937 1102 80-81 0.006832539107428204 1102 82-83 0.2855719140662991 1102 84-85 0.24294847599093927 1102 86-87 0.19561590560947906 1102 88-89 0.2585576173699735 1102 90-91 0.30431727294303457 1102 92-93 0.052375300745215014 1102 94-95 0.18576066037507744 1102 96-97 0.0797435651564733 1102 98-99 0.05603635556748188 1102 100-101 0.3479674656997709 1103 1 0.2464481545291477 1103 2 0.41313376952362546 1103 3 0.2380274987991342 1103 4 0.3743123673737827 1103 5 0.34552681884397174 1103 6 0.30224373178683095 1103 7 0.2909632934585531 1103 8 0.39447784210380377 1103 9 0.35875506233642795 1103 10-11 0.4675189884021549 1103 12-13 0.3845008732606132 1103 14-15 0.37975679346728697 1103 16-17 0.5830588640577119 1103 18-19 0.3881614109186913 1103 20-21 0.5227527823848064 1103 22-23 0.4474229485279295 1103 24-25 0.3803673852033782 1103 26-27 0.3114666260042256 1103 28-29 0.09768468837906141 1103 30-31 0.08980936513906812 1103 32-33 0.3115734045944478 1103 34-35 0.1840655175647541 1103 36-37 0.19478055257722104 1103 38-39 0.21673642515396807 1103 40-41 0.20081915582447607 1103 42-43 0.4546633708878858 1103 44-45 0.3524133798665403 1103 46-47 0.5319684513762368 1103 48-49 0.5158691660694714 1103 50-51 0.45846880694848835 1103 52-53 0.5244463525901679 1103 54-55 0.4927500146592436 1103 56-57 0.6043135473536339 1103 58-59 0.3987963064640496 1103 60-61 0.4371293318287428 1103 62-63 0.5444132145463314 1103 64-65 0.5508393547283745 1103 66-67 0.2594293135721699 1103 68-69 0.4512815370099297 1103 70-71 0.457650904610702 1103 72-73 0.5148069659622152 1103 74-75 0.32850414197708133 1103 76-77 0.3568344155786356 1103 78-79 0.44430169520068574 1103 80-81 0.09886062017100983 1103 82-83 0.1469897107240108 1103 84-85 0.40968696232140545 1103 86-87 0.6171467608669339 1103 88-89 0.7276714434626932 1103 90-91 0.5935301719246375 1103 92-93 0.455485368417186 1103 94-95 0.4067027006314845 1103 96-97 0.2976283825645396 1103 98-99 0.6252324767956736 1103 100-101 1.1977606760642416 1104 1 0.16679709278762544 1104 2 0.28018604380770284 1104 3 0.2644751887317618 1104 4 0.28690527614682537 1104 5 0.3435536256448444 1104 6 0.21469222939957433 1104 7 0.31219428997223275 1104 8 0.2314951509954426 1104 9 0.4978485692017145 1104 10-11 0.4089807104988452 1104 12-13 0.24859271875856592 1104 14-15 0.45086012029146616 1104 16-17 0.36299542538517215 1104 18-19 0.45191063135401777 1104 20-21 0.5070886444044191 1104 22-23 0.33301544323710885 1104 24-25 0.4229450349564772 1104 26-27 0.07652116997167724 1104 28-29 0.45169203417861326 1104 30-31 0.48979185688335036 1104 32-33 0.28699283594674796 1104 34-35 0.17832868837376026 1104 36-37 0.24283943654732099 1104 38-39 0.41258607396648017 1104 40-41 0.5461369114961343 1104 42-43 0.32584925488865935 1104 44-45 0.4615019537488294 1104 46-47 0.5584397838439443 1104 48-49 0.6522258897508522 1104 50-51 0.5063193810147979 1104 52-53 0.3373367739129236 1104 54-55 0.4336110885670834 1104 56-57 0.4307511412847802 1104 58-59 0.38562421947997194 1104 60-61 0.45893158310926907 1104 62-63 0.7766608656285214 1104 64-65 0.6025992539217171 1104 66-67 0.7264460601548706 1104 68-69 0.7201802293860418 1104 70-71 0.7464406368494032 1104 72-73 0.6831303140518976 1104 74-75 0.46197072549018614 1104 76-77 0.6488427275271853 1104 78-79 0.7998093834975037 1104 80-81 0.6107924325438461 1104 82-83 0.6314418684567791 1104 84-85 0.6393288226438649 1104 86-87 0.910150615239143 1104 88-89 0.808188333178574 1104 90-91 0.6022702420599018 1104 92-93 0.549174345117148 1104 94-95 0.5626226636213296 1104 96-97 0.4947298077621163 1104 98-99 1.0075118216546386 1104 100-101 1.371296883648256 1105 1 0.23868049694617 1105 2 0.28319798845561905 1105 3 0.23424335625828974 1105 4 0.30312720278653416 1105 5 0.29303413791342336 1105 6 0.20259420765751202 1105 7 0.30077058419612257 1105 8 0.4479201657378411 1105 9 0.41748993888288055 1105 10-11 0.37843948599394395 1105 12-13 0.31602100988323656 1105 14-15 0.08412082142140775 1105 16-17 0.28788918756583115 1105 18-19 0.17927543100284282 1105 20-21 0.23101178825055513 1105 22-23 0.1869473664126886 1105 24-25 0.39454972184909565 1105 26-27 0.3549455712187637 1105 28-29 0.2039377707828649 1105 30-31 0.2533912232907767 1105 32-33 0.38197288412729336 1105 34-35 0.18330606573419317 1105 36-37 0.21356288502561682 1105 38-39 0.1660118635111445 1105 40-41 0.18131081890594203 1105 42-43 0.6186584023152832 1105 44-45 0.4802406655673366 1105 46-47 0.696819997562443 1105 48-49 0.9055578039355581 1105 50-51 0.8554306061279178 1105 52-53 0.8421713549511551 1105 54-55 0.7898786571423742 1105 56-57 0.8410624901547052 1105 58-59 1.142921375982091 1105 60-61 0.9882788716980002 1105 62-63 1.0176408979427904 1105 64-65 1.2228079745460008 1105 66-67 1.3574458195166201 1105 68-69 1.1631436761719378 1105 70-71 1.1948786037470995 1105 72-73 1.2396584859152036 1105 74-75 1.2531806863269708 1105 76-77 0.9132083019782797 1105 78-79 1.0585873907483858 1105 80-81 0.852848726027311 1105 82-83 1.054761565622801 1105 84-85 0.9953226982682679 1105 86-87 1.1163623874737176 1105 88-89 0.8466673143532581 1105 90-91 0.8428205632194086 1105 92-93 0.8670118534799869 1105 94-95 0.8039517536121039 1105 96-97 0.9909324833420925 1105 98-99 0.8665374747494852 1105 100-101 1.3593337250993827 1106 1 0.0089772042795353 1106 2 0.016427150484204844 1106 3 0.09636154941025765 1106 4 0.08618188019870843 1106 5 0.11824479956354139 1106 6 0.06217444275257833 1106 7 0.20335139812372915 1106 8 0.2641143974489566 1106 9 0.31503518800349894 1106 10-11 0.21924314488347107 1106 12-13 0.4641805728646702 1106 14-15 0.5587113252283515 1106 16-17 0.4927437280618463 1106 18-19 0.3988936798117919 1106 20-21 0.36293342879555013 1106 22-23 0.24525013479063063 1106 24-25 0.281864523265007 1106 26-27 -0.044481579366745905 1106 28-29 0.36564980716025985 1106 30-31 0.14447400190960025 1106 32-33 -0.14148166082128455 1106 34-35 -0.05104110577833865 1106 36-37 -0.10171787658442355 1106 38-39 -0.200487924992359 1106 40-41 -0.1998441468093688 1106 42-43 -0.25852682966298346 1106 44-45 -0.3140094123524264 1106 46-47 -0.41433254707444434 1106 48-49 -0.26091846949603337 1106 50-51 -0.2549796136058262 1106 52-53 -0.0065481171980934505 1106 54-55 -0.03541479425795302 1106 56-57 -0.16415187268424347 1106 58-59 -0.04703935802209003 1106 60-61 0.004352606058382946 1106 62-63 -0.06779951569983922 1106 64-65 -0.21964340908639457 1106 66-67 -0.09766474306408668 1106 68-69 -0.11261715467538735 1106 70-71 -0.10739094854819342 1106 72-73 0.16654887506877358 1106 74-75 0.016388782190542628 1106 76-77 0.23518320624828348 1106 78-79 0.177097787471304 1106 80-81 0.08741017689333574 1106 82-83 0.15747720857217473 1106 84-85 -0.013570590613696254 1106 86-87 0.1570928574412136 1106 88-89 0.1562467145936992 1106 90-91 0.33661202766572984 1106 92-93 0.20531860133162994 1106 94-95 0.20491990669104965 1106 96-97 0.24014215429805574 1106 98-99 0.7957598911527057 1106 100-101 1.3475309678528475 1107 1 0.13545489924127097 1107 2 0.18300304511861043 1107 3 0.029388377383892106 1107 4 0.10975311919548147 1107 5 0.12362891731468295 1107 6 0.13734750861713252 1107 7 0.23739782179780633 1107 8 0.41095912474581553 1107 9 0.22050279759344704 1107 10-11 0.2865929978051369 1107 12-13 0.22774039393779333 1107 14-15 0.17903840865383813 1107 16-17 0.27522191512780125 1107 18-19 0.30032769361282163 1107 20-21 0.28440617506882404 1107 22-23 0.5356586642284142 1107 24-25 0.4856668577189467 1107 26-27 0.32478665060626355 1107 28-29 0.25399951632113726 1107 30-31 0.3498632342270156 1107 32-33 0.05876397659190502 1107 34-35 0.16434088320737317 1107 36-37 0.24076799615411204 1107 38-39 0.3449667461272128 1107 40-41 0.3054051952085928 1107 42-43 0.3005650721815698 1107 44-45 0.37757118215369445 1107 46-47 0.1541805240982015 1107 48-49 0.2848230320300402 1107 50-51 0.22010069890800565 1107 52-53 0.32119674611858073 1107 54-55 0.3015584054147169 1107 56-57 0.3568669212733866 1107 58-59 0.21573392569533922 1107 60-61 0.31181268479505775 1107 62-63 0.36363903209508663 1107 64-65 0.39500927039340894 1107 66-67 0.46235054534216147 1107 68-69 0.6662262345403427 1107 70-71 0.6596384837073046 1107 72-73 0.32600584636085017 1107 74-75 0.576554742954908 1107 76-77 0.4551683048902966 1107 78-79 0.3667016712241207 1107 80-81 0.5827787297751854 1107 82-83 0.5143654235056019 1107 84-85 0.6091589566504254 1107 86-87 0.6377535764662952 1107 88-89 0.569980569152019 1107 90-91 0.6453521302888099 1107 92-93 0.5504149763482182 1107 94-95 0.6077168832912143 1107 96-97 0.8073669076930194 1107 98-99 0.8402801171888896 1107 100-101 1.388569833027347 1108 1 0.07248051463687233 1108 2 0.17640697090865487 1108 3 0.15153795064370357 1108 4 0.07731647116551699 1108 5 0.1689034699789289 1108 6 0.08850992252765622 1108 7 0.009842872819895376 1108 8 0.08400034883658947 1108 9 0.11210008007677885 1108 10-11 -0.018561249275784064 1108 12-13 0.27159316582676496 1108 14-15 0.17128898258368963 1108 16-17 0.29759419513965923 1108 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-1.844087551181481 2316 68-69 -2.4203340210262745 2316 70-71 -2.609226708276992 2316 72-73 -2.0077094683261656 2316 74-75 -1.7228525532170273 2316 76-77 -1.5095891111722288 2316 78-79 -2.218164743100509 2316 80-81 -1.9598363286328109 2316 82-83 -1.5553253168914303 2316 84-85 -1.2432650079691925 2316 86-87 -2.0217749601186163 2316 88-89 -2.2671041491566477 2316 90-91 -2.362435602438598 2316 92-93 -2.6708529955229565 2316 94-95 -2.7682900945473037 2316 96-97 -3.9634428740292407 2316 98-99 -5.526647890340126 2316 100-101 -6.135904626246408 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2008.0 3 836.0 4 831.0 5 927.0 6 1023.0 7 1220.0 8 1531.0 9 1952.0 10 2688.0 11 3652.0 12 5345.0 13 7110.0 14 8383.0 15 11285.0 16 15152.0 17 20280.0 18 15723.0 19 8649.0 20 7915.0 21 8393.0 22 9123.0 23 10134.0 24 11222.0 25 12678.0 26 14457.0 27 17333.0 28 20480.0 29 24501.0 30 30095.0 31 36975.0 32 47130.0 33 60926.0 34 82262.0 35 112513.0 36 150121.0 37 188634.0 38 144940.0 39 23046.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.689602698804464 18.605391721799073 14.430165669273359 20.274839910123106 2 15.819577385900258 21.126691473155834 39.99323945872859 23.060491682215318 3 19.45621210688823 28.094280826865827 29.787397234861928 22.662109831384015 4 12.35246257032283 16.79566991583564 37.49417879529165 33.35768871854988 5 12.578670782737483 39.637672754525745 32.15683300470196 15.626823458034814 6 29.412688330133534 36.52908723673035 17.870843533433728 16.187380899702383 7 27.342274466560855 35.64721510732126 20.014952443912723 16.995557982205163 8 27.831267155579305 32.046109201590376 19.663494176135224 20.459129466695096 9 29.352715010838633 15.785600228369567 20.088939241206432 34.77274551958537 10-11 25.235092727860735 27.205986327798286 25.426771196931004 22.132149747409976 12-13 27.888028068810694 25.21741418846979 26.53463394529419 20.35992379742533 14-15 24.449323979256178 25.252886128874135 27.32655461577678 22.971235276092912 16-17 21.175216887252528 29.029370162719527 27.536261621628373 22.259151328399575 18-19 21.180457510479577 26.224349735317325 32.10799106587246 20.487201688330632 20-21 23.141266053974082 24.595247894361584 31.866806916223485 20.396679135440852 22-23 22.320089186176144 24.991170568561873 31.805128205128202 20.883612040133777 24-25 21.8226453607227 25.287484444785285 32.132224786953195 20.75764540753882 26-27 21.24488339947385 25.374548535247694 32.5023409283453 20.878227136933162 28-29 21.077621376755054 25.836472995929217 32.295602391642625 20.790303235673107 30-31 21.23870524736428 25.624669768520274 32.002256163763604 21.134368820351845 32-33 20.9065418018925 25.92528355625625 31.748625522938863 21.419549118912386 34-35 21.68422967546556 25.679178805608394 31.74378963269725 20.8928018862288 36-37 21.726884118617775 25.472804832318424 31.63974490052984 21.160566148533956 38-39 21.53625195580821 25.732884643767566 31.553378793844328 21.1774846065799 40-41 21.419915573888897 25.926743317157904 31.435127967798948 21.21821314115425 42-43 21.16615676269982 26.334407237753165 30.65870794406899 21.840728055478024 44-45 21.31642363998315 26.09729835770288 30.563089221742434 22.023188780571537 46-47 21.89705944639748 26.575373002904662 29.641921669947322 21.88564588075053 48-49 21.87668167532871 26.973013388309113 28.90040558609353 22.249899350268645 50-51 21.92322062145054 27.31220457380606 28.718167980860887 22.04640682388252 52-53 22.243207165079866 27.36546526382621 28.14080837210049 22.250519198993434 54-55 21.482002988243657 27.279739645593054 28.44899810013336 22.78925926602993 56-57 22.091534103222457 27.492470705537475 27.642566087005378 22.773429104234687 58-59 22.377480815620387 26.931257273566356 28.014634081089397 22.676627829723863 60-61 22.118905402537273 27.19090712900919 28.675708647163006 22.01447882129054 62-63 22.29272923212605 27.165381890429074 28.534160155840706 22.007728721604174 64-65 21.989126838981093 27.46574383603052 28.519532723363923 22.025596601624464 66-67 21.97395912754784 27.417823042988577 28.07276855262794 22.535449276835646 68-69 22.18384864171646 27.41886217304855 28.324964226359633 22.072324958875363 70-71 22.20437284481029 27.506262144190273 27.88395259229139 22.40541241870805 72-73 21.951596852662966 27.661272488621936 27.952679373956713 22.434451284758385 74-75 21.74598496667013 28.290552828658473 27.71794159570517 22.245520608966228 76-77 21.693731516040277 27.669771533464743 27.997171421908906 22.63932552858607 78-79 21.63924164073974 27.676492592879924 28.18062975457032 22.503636011810016 80-81 21.791463104149845 28.24079626923802 27.576123872426773 22.39161675418536 82-83 21.82042169214574 27.99122176936991 27.455022045896666 22.733334492587684 84-85 21.520535636648212 27.98121793571201 27.385962056841162 23.112284370798616 86-87 21.53665476809375 28.167738667746246 27.230625731771152 23.064980832388848 88-89 21.611418487180643 27.688948595495223 27.21209335216083 23.487539565163306 90-91 21.57191806015966 27.59243200103439 27.515432270778668 23.320217668027276 92-93 21.70137890225593 27.64087451021836 27.553207665631696 23.10453892189401 94-95 21.33165738399409 27.464954213854764 27.74671167770763 23.45667672444352 96-97 21.162154124246626 27.267830684575046 27.94516907598666 23.62484611519167 98-99 21.18877314401912 27.485284149691413 27.44510541900039 23.880837287289072 100-101 21.362426694604 27.477063422296617 27.529780421655715 23.63072946144367 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 556.0 1 488.5 2 578.0 3 778.5 4 754.5 5 779.0 6 1156.0 7 2213.0 8 3620.0 9 4215.0 10 3576.5 11 2800.5 12 2695.0 13 2861.0 14 2963.0 15 2996.5 16 3048.5 17 3148.5 18 3300.0 19 3431.0 20 3572.0 21 3865.5 22 4361.5 23 4893.0 24 5428.0 25 6195.5 26 7317.0 27 8721.5 28 9976.5 29 11595.5 30 13772.5 31 15831.5 32 18625.0 33 20870.5 34 22435.5 35 23785.0 36 26124.5 37 28926.0 38 29867.0 39 29926.0 40 29400.5 41 29398.5 42 29686.5 43 30962.5 44 32519.0 45 34454.0 46 35954.0 47 38358.0 48 46946.0 49 47062.0 50 39410.0 51 35441.0 52 32828.5 53 33013.5 54 33318.0 55 32216.5 56 29946.5 57 27589.0 58 26754.5 59 24757.5 60 21724.0 61 19742.0 62 17261.5 63 12754.0 64 8842.0 65 6902.5 66 5075.5 67 3866.0 68 2973.5 69 2162.5 70 1631.0 71 1289.0 72 1133.0 73 1120.0 74 929.5 75 715.5 76 554.0 77 334.0 78 220.0 79 163.0 80 104.0 81 63.5 82 41.5 83 21.5 84 11.0 85 12.5 86 10.0 87 7.0 88 5.5 89 5.5 90 6.0 91 2.5 92 1.5 93 1.5 94 1.5 95 1.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0345972320431003 2 0.02362955281294222 3 0.02059783660297982 4 0.051271671197893484 5 0.059475138589556456 6 0.05109333495024865 7 0.05260919305522984 8 0.0433357081776978 9 0.04342487630152023 10-11 0.05256460899331863 12-13 0.046144504078104144 14-15 0.04177526601080539 16-17 0.04997873340246835 18-19 0.054080467098299834 20-21 0.012706457644695343 22-23 0.020241164107690127 24-25 0.006108016481836007 26-27 0.01132435172544778 28-29 0.0064201049152144895 30-31 0.009318068939443251 32-33 0.004502990253032385 34-35 0.007668458648728418 36-37 0.009853077682377793 38-39 0.012260617025583226 40-41 0.00205086684791574 42-43 0.0021400349717381633 44-45 0.0026304596527614924 46-47 4.012565572009056E-4 48-49 0.0012037696716027167 50-51 3.5667249528969385E-4 52-53 0.0033883887052520915 54-55 0.007356370215349935 56-57 0.016941943526260456 58-59 0.010878511106335663 60-61 0.0102543342395787 62-63 0.006732193348592971 64-65 0.0 66-67 0.007668458648728418 68-69 0.01698652758817167 70-71 0.004993414934055714 72-73 0.0018725306002708927 74-75 0.0031654683956960326 76-77 0.005840512110368737 78-79 0.004993414934055714 80-81 0.008693892072686287 82-83 0.00592968023419116 84-85 0.017343200083461362 86-87 0.004815078686410867 88-89 0.006018848358013584 90-91 0.003967981510097844 92-93 0.016629855092881975 94-95 0.010923095168246875 96-97 0.009139732691798404 98-99 0.02113284534591436 100-101 0.035355161095590906 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1121477.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.003191770609995 #Duplication Level Percentage of deduplicated Percentage of total 1 72.13989619754308 33.90805375285017 2 14.120439153472367 13.274114188317832 3 5.467978591142302 7.710373389511546 4 2.7144318013860023 5.103478340351544 5 1.5162604470592689 3.563454028365883 6 0.9207320687787377 2.596640759889449 7 0.6197027827548635 2.0389606117025263 8 0.41524626842067086 1.561431998928559 9 0.3103985370801473 1.3130749767325467 >10 1.582122298309533 13.36470070330929 >50 0.12072251620217479 3.8697403163654296 >100 0.06329899584955534 5.9292704170833215 >500 0.0061011074792034 1.8916166708452444 >1k 0.0020972556959761687 1.6119248180070784 >5k 3.813192174502125E-4 1.25507350176178 >10k+ 1.9065960872510624E-4 1.008091525977741 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11249 1.0030522248784415 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8682 0.7741576510262805 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5323 0.47464192310676007 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2944 0.2625109565332147 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2038 0.18172463635009903 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1981 0.17664205329222088 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1901 0.169508603386427 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1709 0.1523883236125217 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1352 0.12055530340791654 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1349 0.12028779903644925 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1319 0.11761275532177655 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1244 0.11092514603509478 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1142 0.10182999740520761 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 8.916812382242346E-5 0.0 0.0 0.0 0.0 14-15 8.916812382242346E-5 0.0 0.0 0.0 0.0 16-17 8.916812382242346E-5 0.0 0.0 0.0 0.0 18-19 8.916812382242346E-5 0.0 0.0 0.0 0.0 20-21 8.916812382242346E-5 0.0 0.0 0.0 0.0 22-23 1.7833624764484693E-4 0.0 0.0 4.458406191121173E-5 0.0 24-25 1.7833624764484693E-4 0.0 0.0 1.3375218573363518E-4 0.0 26-27 1.7833624764484693E-4 0.0 0.0 4.012565572009056E-4 0.0 28-29 2.675043714672704E-4 0.0 0.0 0.0010254334239578698 0.0 30-31 2.675043714672704E-4 0.0 0.0 0.0020508668479157395 0.0 32-33 2.675043714672704E-4 0.0 0.0 0.003700477138630574 0.0 34-35 2.675043714672704E-4 0.0 0.0 0.0047259105625884435 0.0 36-37 2.675043714672704E-4 0.0 0.0 0.006063432419924795 0.0 38-39 2.675043714672704E-4 0.0 0.0 0.008203467391662958 0.0 40-41 2.675043714672704E-4 0.0 0.0 0.012305201087494437 0.0 42-43 3.5667249528969385E-4 0.0 0.0 0.017610704454928634 0.0 44-45 3.5667249528969385E-4 0.0 0.0 0.03161009989504912 0.0 46-47 3.5667249528969385E-4 0.0 0.0 0.05885096172279949 0.0 48-49 3.5667249528969385E-4 0.0 0.0 0.08203467391662958 0.0 50-51 3.5667249528969385E-4 0.0 0.0 0.11217349976860871 0.0 52-53 3.5667249528969385E-4 0.0 0.0 0.14833117397860143 0.0 54-55 3.5667249528969385E-4 0.0 0.0 0.19068603279425256 0.0 56-57 3.5667249528969385E-4 0.0 0.0 0.2367859528104455 0.0 58-59 3.5667249528969385E-4 0.0 0.0 0.30713960250633765 0.0 60-61 3.5667249528969385E-4 0.0 0.0 0.3919830723233736 0.0 62-63 3.5667249528969385E-4 0.0 0.0 0.4931888928618242 0.0 64-65 3.5667249528969385E-4 0.0 0.0 0.5757139914594771 0.0 66-67 3.5667249528969385E-4 0.0 0.0 0.6965367992388609 0.0 68-69 3.5667249528969385E-4 0.0 0.0 0.8489697069132938 0.0 70-71 3.5667249528969385E-4 0.0 0.0 0.9712192046738364 0.0 72-73 3.5667249528969385E-4 0.0 0.0 1.1456320548704966 0.0 74-75 3.5667249528969385E-4 0.0 0.0 1.2934282201061635 0.0 76-77 3.5667249528969385E-4 0.0 0.0 1.432084652650032 0.0 78-79 3.5667249528969385E-4 0.0 0.0 1.6130959440095518 0.0 80-81 4.4584061911211734E-4 0.0 0.0 1.805609923342164 0.0 82-83 4.4584061911211734E-4 0.0 0.0 2.035396178432549 0.0 84-85 4.4584061911211734E-4 0.0 0.0 2.3460579218298725 0.0 86-87 4.4584061911211734E-4 0.0 0.0 2.73634679980062 0.0 88-89 4.4584061911211734E-4 0.0 0.0 3.1842828698225643 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTACGAG 65 0.0058290097 29.24615 3 GTACATG 12035 0.0 28.786184 1 GTATCAA 15280 0.0 28.67545 1 TACATGG 12155 0.0 27.994976 2 GGTATCA 10645 0.0 27.232458 1 TCGACCG 35 0.008381763 27.150473 18-19 ACATGGG 12070 0.0 26.49902 3 ATGGGAG 2635 0.0 24.88975 5 CATGGGA 7940 0.0 24.781137 4 GAGTACT 8810 0.0 24.358057 12-13 CATGCGT 100 0.0016481373 23.763556 4 ATCAACG 18425 0.0 23.730255 3 TCAACGC 18545 0.0 23.577753 4 ATGGGAT 2595 0.0 23.442 5 CAACGCA 18665 0.0 23.425123 5 TATCAAC 18720 0.0 23.381687 2 TATAGGG 265 3.092282E-11 23.314146 2 AGTACTT 8975 0.0 23.221804 12-13 GTACTTT 9205 0.0 23.078403 14-15 AACGCAG 19075 0.0 22.946537 6 >>END_MODULE