FastQCFastQC Report
Thu 26 May 2016
SRR936409_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936409_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121477
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122381.0912394993388184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101700.9068398192740466No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67610.602865685163405No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34220.30513331972033314No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24640.21971025709845143No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22660.20205496858161157No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19920.17762290265426756No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16740.1492674392787369No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16410.1463248911925969No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13920.12412202836081347No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13510.1204661352840941No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12310.10976596042540328No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG11940.10646673984397362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116000.027.5299551
GTATCAA162500.026.9632231
GTACATG113950.024.688891
TACATGG115850.023.9451392
ACATGGG113950.023.2584973
GAGTACT102250.023.18233312-13
TAAGGTG5150.022.1333145
ATCAACG199200.021.9838333
TATCAAC199100.021.9729772
TCAACGC200150.021.8785114
GTACTTT108300.021.79859514-15
ATCACGC1100.002878546221.5893273
CAACGCA203000.021.5713525
CATGGGA74400.021.4490874
AACGCAG205950.021.3334526
AGTACTT104050.021.20623412-13
GGGACCG1805.1738407E-621.1086255
GTAAGGT4400.020.5089454
ATGGGAG25050.020.476635
ATGGGAT24950.020.3683435
Produced by FastQC (version 0.11.5)