##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936409_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1121477 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.827351786973786 31.0 31.0 34.0 30.0 34.0 2 31.939027728611464 31.0 31.0 34.0 30.0 34.0 3 32.014938335783974 33.0 31.0 34.0 30.0 34.0 4 35.810695181443755 37.0 35.0 37.0 35.0 37.0 5 35.605464044291594 37.0 35.0 37.0 33.0 37.0 6 35.73017012386344 37.0 35.0 37.0 33.0 37.0 7 35.669932597815205 37.0 35.0 37.0 33.0 37.0 8 35.656383501400384 37.0 35.0 37.0 33.0 37.0 9 37.24689850973315 39.0 37.0 39.0 34.0 39.0 10-11 37.119071991668136 39.0 37.0 39.0 33.0 39.0 12-13 37.00852982272485 39.0 37.0 39.0 33.0 39.0 14-15 37.974600905769805 40.0 37.0 41.0 33.0 41.0 16-17 37.941007706800946 40.0 37.0 41.0 33.0 41.0 18-19 38.178618464756745 40.0 37.5 41.0 33.0 41.0 20-21 38.2470674833278 40.0 38.0 41.0 33.0 41.0 22-23 38.14770699711184 40.0 37.0 41.0 33.0 41.0 24-25 37.998025817738565 40.0 37.0 41.0 33.0 41.0 26-27 37.73220716965216 40.0 37.0 41.0 32.5 41.0 28-29 37.60778152382973 39.0 36.5 41.0 32.5 41.0 30-31 37.43483548926996 39.0 36.0 41.0 32.0 41.0 32-33 37.14875249336366 39.0 35.0 41.0 32.0 41.0 34-35 36.90458431158196 39.0 35.0 41.0 31.0 41.0 36-37 36.56060088615281 39.0 35.0 41.0 30.5 41.0 38-39 36.322682944010445 39.0 35.0 40.0 30.0 41.0 40-41 36.0056545965722 38.0 35.0 40.0 30.0 41.0 42-43 35.61068483794139 38.0 35.0 40.0 28.5 41.0 44-45 35.13548561406074 38.0 34.5 40.0 26.5 41.0 46-47 34.484622511206204 38.0 34.0 40.0 23.5 41.0 48-49 33.89648784593888 38.0 33.0 40.0 20.5 41.0 50-51 33.49261643350688 38.0 33.0 40.0 17.5 41.0 52-53 33.179072330507 37.5 33.0 40.0 14.5 41.0 54-55 32.888929064082454 37.0 33.0 40.0 12.0 41.0 56-57 32.54908348543929 37.0 32.0 40.0 8.0 41.0 58-59 32.1969670354363 36.0 31.5 39.5 7.0 41.0 60-61 31.816105011516065 36.0 31.0 39.0 4.0 40.0 62-63 31.407662395216306 35.0 31.0 39.0 2.0 40.0 64-65 30.981100816155838 35.0 30.0 38.5 2.0 40.0 66-67 30.517922347047687 35.0 29.5 38.0 2.0 40.0 68-69 30.2409581293241 35.0 29.0 37.5 2.0 40.0 70-71 29.814582465801795 34.0 29.0 37.0 2.0 39.0 72-73 29.375561424799617 34.0 29.0 36.0 2.0 39.0 74-75 28.87300809557396 34.0 28.0 36.0 2.0 38.5 76-77 27.49852828011631 32.0 26.0 34.5 2.0 36.5 78-79 28.24779063681199 33.5 27.0 35.0 2.0 37.0 80-81 28.053605646838946 34.0 27.0 35.0 2.0 37.0 82-83 27.721350950576785 33.0 26.5 35.0 2.0 36.0 84-85 27.303243401335916 33.0 26.0 35.0 2.0 36.0 86-87 26.93847354872191 33.0 25.5 35.0 2.0 35.5 88-89 26.522625965579323 33.0 25.0 35.0 2.0 35.0 90-91 26.02609014718982 32.5 24.0 35.0 2.0 35.0 92-93 25.538558971784532 32.0 22.0 34.0 2.0 35.0 94-95 24.95005381296273 32.0 19.0 34.0 2.0 35.0 96-97 24.206030975222852 31.5 7.5 34.0 2.0 35.0 98-99 23.295219607713754 31.0 2.0 34.0 2.0 35.0 100-101 20.31063811384451 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3030547278614222 1101 2 0.39725275417833217 1101 3 0.3782738398762149 1101 4 0.25808861321976906 1101 5 0.20421892705475386 1101 6 0.27238066816958906 1101 7 0.2075368378565443 1101 8 0.23510976161160357 1101 9 0.31820595188504086 1101 10-11 0.3537919638836442 1101 12-13 0.36825752395625955 1101 14-15 0.5646623384667606 1101 16-17 0.44166465615616346 1101 18-19 0.5588865977549631 1101 20-21 0.32307811986373025 1101 22-23 0.40805278297798253 1101 24-25 0.31733449046250684 1101 26-27 0.21039699065104145 1101 28-29 0.20607009087486006 1101 30-31 0.48402113365265365 1101 32-33 0.4633909887702501 1101 34-35 0.4023657386043098 1101 36-37 0.3734692076635113 1101 38-39 0.6287439360110767 1101 40-41 0.6480549788125174 1101 42-43 0.8558274957255421 1101 44-45 0.8790446494305755 1101 46-47 0.8510340644498768 1101 48-49 0.8743934832408371 1101 50-51 0.7602086823319851 1101 52-53 0.7089855303233534 1101 54-55 0.8298064152673348 1101 56-57 0.6932763792913761 1101 58-59 0.6920397974791825 1101 60-61 0.693694000892723 1101 62-63 0.8632804750336298 1101 64-65 0.809097116567731 1101 66-67 0.6004118235498659 1101 68-69 0.48312077206392345 1101 70-71 0.3867355701714672 1101 72-73 0.43532388225814955 1101 74-75 0.4135935851884973 1101 76-77 0.3855652184838618 1101 78-79 0.11694708934686915 1101 80-81 0.31801830077887416 1101 82-83 0.733919914805341 1101 84-85 0.7334324045469778 1101 86-87 0.5294713381128524 1101 88-89 0.29948437392019045 1101 90-91 0.2862533128670641 1101 92-93 0.17451475782113235 1101 94-95 0.5431348328859116 1101 96-97 0.6369018995581364 1101 98-99 0.35996899912499813 1101 100-101 1.1880175123175611 1102 1 0.33362088771362153 1102 2 0.3561802826745577 1102 3 0.3585388892748327 1102 4 0.2641372524532173 1102 5 0.214127014555352 1102 6 0.18348700895880654 1102 7 0.2323231725603847 1102 8 0.2116952167712185 1102 9 0.22138131020712848 1102 10-11 0.24117916833319697 1102 12-13 0.2833470835266212 1102 14-15 0.37922155427741444 1102 16-17 0.43236961948270647 1102 18-19 0.3666703448814914 1102 20-21 0.23309953474120704 1102 22-23 0.24530841679260362 1102 24-25 0.26714264616581573 1102 26-27 -0.017775241176288148 1102 28-29 -0.11233406884583275 1102 30-31 0.1286170035853189 1102 32-33 0.3018101943440499 1102 34-35 0.1912609449465137 1102 36-37 0.4121391129919658 1102 38-39 0.27066262394048124 1102 40-41 0.29879038194984275 1102 42-43 0.15967508265176633 1102 44-45 0.5178204043340244 1102 46-47 0.2654245140075915 1102 48-49 0.20497840206157036 1102 50-51 0.022924168730774852 1102 52-53 0.013302413781808298 1102 54-55 0.24752480898391838 1102 56-57 0.3198468788472226 1102 58-59 0.20042299356907023 1102 60-61 0.3688063935443644 1102 62-63 0.1582188411854908 1102 64-65 0.13639599270567615 1102 66-67 0.26811861378076074 1102 68-69 0.09949503089127276 1102 70-71 0.24789728537293598 1102 72-73 0.1287195005240953 1102 74-75 0.24405215494285315 1102 76-77 0.057520496071468585 1102 78-79 0.1665577924020667 1102 80-81 0.46331682623370085 1102 82-83 0.46471646831223623 1102 84-85 0.2712236681536737 1102 86-87 0.48798017416720185 1102 88-89 0.3793176447236313 1102 90-91 0.4529425418670101 1102 92-93 0.6616464921941336 1102 94-95 0.9038797895597845 1102 96-97 1.134131567350341 1102 98-99 0.8279317751429147 1102 100-101 1.5096190769831423 1103 1 0.09712659673943946 1103 2 0.16906881005697727 1103 3 0.08902391423747957 1103 4 0.14193375717292156 1103 5 -0.050756867072244916 1103 6 0.07886445741438308 1103 7 0.10396698442516339 1103 8 0.16624866844976083 1103 9 0.14792891771988792 1103 10-11 -0.004732529177672973 1103 12-13 0.16873023080877658 1103 14-15 0.1241917342430412 1103 16-17 0.2309011198422226 1103 18-19 0.04841191648272769 1103 20-21 0.21179905486685868 1103 22-23 0.04218062793731292 1103 24-25 0.13789243070682033 1103 26-27 0.26057673977152973 1103 28-29 0.3132567222583873 1103 30-31 0.06499285585949366 1103 32-33 0.11575657437801823 1103 34-35 0.05058247860656451 1103 36-37 0.16375378052216405 1103 38-39 0.15064909719552588 1103 40-41 0.09235198282289758 1103 42-43 0.1245466614434676 1103 44-45 0.12837732120510026 1103 46-47 0.0434284458154508 1103 48-49 -0.016688421843412016 1103 50-51 0.05221766722383592 1103 52-53 -0.012603980123124359 1103 54-55 -0.008600792133911739 1103 56-57 0.08155114672012331 1103 58-59 0.031961154033744776 1103 60-61 0.07301785188301224 1103 62-63 -0.05013032222166913 1103 64-65 0.022546632963106106 1103 66-67 0.14693376781481504 1103 68-69 0.22054346199284325 1103 70-71 0.2789982808189748 1103 72-73 0.07194988100682664 1103 74-75 0.10227631388784175 1103 76-77 -0.10803009726944168 1103 78-79 0.05757410863976986 1103 80-81 0.1639225337296324 1103 82-83 0.27056783730552425 1103 84-85 0.28152362786666885 1103 86-87 0.13644193720820752 1103 88-89 0.055792901362064384 1103 90-91 -0.18756062499769044 1103 92-93 -0.15800508894338705 1103 94-95 0.0018457419395225827 1103 96-97 0.023111859864606288 1103 98-99 0.19394273677846385 1103 100-101 0.20422826752028556 1104 1 0.10043016177813513 1104 2 0.21642289088632793 1104 3 0.15153733297361072 1104 4 0.034770452432141497 1104 5 -0.003818128075607774 1104 6 0.06048973473492936 1104 7 0.029806102724734274 1104 8 -0.10643794144074548 1104 9 0.04914657217592833 1104 10-11 0.0726507661572029 1104 12-13 0.01636431593212251 1104 14-15 0.07062861250826558 1104 16-17 0.16237802428037895 1104 18-19 0.1568787555908031 1104 20-21 0.06487667394855379 1104 22-23 -0.009405466673719332 1104 24-25 0.006940262750255499 1104 26-27 0.07018276567863069 1104 28-29 0.10595002873721171 1104 30-31 0.0769930398878742 1104 32-33 0.15964670432250472 1104 34-35 0.1874861755322641 1104 36-37 0.3511342898718226 1104 38-39 0.3307074495280844 1104 40-41 0.40391075433121415 1104 42-43 0.36465833088760036 1104 44-45 0.7569767374261787 1104 46-47 0.6532921114563308 1104 48-49 0.5580213500636262 1104 50-51 0.5105556864650183 1104 52-53 0.32522803774808295 1104 54-55 0.400824655924076 1104 56-57 0.5539558353543228 1104 58-59 0.44747231842221424 1104 60-61 0.4150722987243647 1104 62-63 0.392002336681724 1104 64-65 0.7177975505490615 1104 66-67 0.3082039725975072 1104 68-69 0.6175974743227464 1104 70-71 0.5630556721144337 1104 72-73 0.6629327459197718 1104 74-75 0.6577429791913367 1104 76-77 0.4902531990878174 1104 78-79 0.4789460657816029 1104 80-81 0.4761714116676927 1104 82-83 0.42264492986157265 1104 84-85 0.5743869050031876 1104 86-87 0.5319604627319201 1104 88-89 0.7839887331192266 1104 90-91 0.8120821810261738 1104 92-93 0.8940985854899601 1104 94-95 0.8686085549155678 1104 96-97 0.8958057542118674 1104 98-99 0.9353464388160688 1104 100-101 1.2109734188836292 1105 1 0.0627676317975201 1105 2 0.16018361713784657 1105 3 0.05751256574483676 1105 4 0.05691974863137972 1105 5 0.016821229235162605 1105 6 0.046773354674584766 1105 7 0.15578022485790655 1105 8 0.05716830034516107 1105 9 0.02309549869135452 1105 10-11 0.07011282596771196 1105 12-13 -0.17481285463807694 1105 14-15 -0.005797169103267663 1105 16-17 0.11541918928681838 1105 18-19 0.11268593824580364 1105 20-21 0.21972804677653102 1105 22-23 0.19559055353989407 1105 24-25 0.17833815941331466 1105 26-27 0.34091467616120497 1105 28-29 0.09177617157055806 1105 30-31 0.27652445640094925 1105 32-33 0.32768888741383506 1105 34-35 0.2105861250246761 1105 36-37 0.152550882275591 1105 38-39 0.4133190884860909 1105 40-41 0.360926416972255 1105 42-43 0.3261548892317947 1105 44-45 0.35736122974558526 1105 46-47 0.1798687547378961 1105 48-49 0.003221939230677151 1105 50-51 0.13704017323124162 1105 52-53 0.06823828999513637 1105 54-55 0.22113994491474642 1105 56-57 0.1452152460286129 1105 58-59 0.24114816019330476 1105 60-61 0.07766309999683685 1105 62-63 0.19976146572993514 1105 64-65 0.16808790307087307 1105 66-67 0.5424520838517104 1105 68-69 0.5841044647315918 1105 70-71 0.5482974077772873 1105 72-73 0.47016366079465044 1105 74-75 0.2789387132834307 1105 76-77 0.34183493279383015 1105 78-79 0.25174521746567535 1105 80-81 0.518255999977967 1105 82-83 0.5089943947720208 1105 84-85 0.3443836788640553 1105 86-87 0.21587154822196553 1105 88-89 0.38486574053141354 1105 90-91 0.41823075266629317 1105 92-93 0.4935344077984496 1105 94-95 0.3534463620929351 1105 96-97 0.5601643210656668 1105 98-99 0.7179244941945981 1105 100-101 0.9188894916674428 1106 1 0.11455644697183587 1106 2 0.14983141980047776 1106 3 0.08431139917464492 1106 4 0.004447744089773664 1106 5 -0.11621563794503231 1106 6 -0.07201671834736345 1106 7 0.04880473618966619 1106 8 0.011919179725367712 1106 9 0.0577294427672328 1106 10-11 0.050690628852969155 1106 12-13 0.08671579200691326 1106 14-15 0.23903508077428626 1106 16-17 0.21752747549724205 1106 18-19 0.08597754740026176 1106 20-21 -0.0010979583884349609 1106 22-23 -0.1580636051492732 1106 24-25 -0.11624446893167573 1106 26-27 0.011284154989290585 1106 28-29 -0.11561980554329665 1106 30-31 0.04612127727454407 1106 32-33 0.021455167078997306 1106 34-35 0.031993328150370814 1106 36-37 -0.13833403579734238 1106 38-39 0.18776015331533813 1106 40-41 0.19299053937307775 1106 42-43 0.2041833243802884 1106 44-45 0.11544749249103603 1106 46-47 0.16657786912411154 1106 48-49 0.011973998538749697 1106 50-51 -0.07088871941758867 1106 52-53 -0.2090071906584967 1106 54-55 -0.1679440525918281 1106 56-57 -0.06318116520964878 1106 58-59 -0.059986685525558414 1106 60-61 0.0014290457201582285 1106 62-63 0.004397135172030886 1106 64-65 0.03355595040156345 1106 66-67 0.07597706270206217 1106 68-69 -0.049261474312888964 1106 70-71 0.2407682713509054 1106 72-73 0.09139707501612193 1106 74-75 0.15743049274823306 1106 76-77 -0.1772707152759878 1106 78-79 0.012229600118509154 1106 80-81 -0.09929779248830783 1106 82-83 0.44997156428090435 1106 84-85 0.15570862098079985 1106 86-87 0.20854987316964824 1106 88-89 0.29818348063560407 1106 90-91 0.44934439307770546 1106 92-93 0.2784243739590089 1106 94-95 0.40539519107018407 1106 96-97 0.38258100257419514 1106 98-99 1.0119164628931152 1106 100-101 1.2724817319719968 1107 1 -0.01744359726225042 1107 2 0.013103857541000963 1107 3 0.008853576376395722 1107 4 -0.014192776545428387 1107 5 -0.1287836478534672 1107 6 0.015490746154782187 1107 7 0.00971562662183345 1107 8 -0.0980799355928994 1107 9 -0.01874991097276535 1107 10-11 -0.018131389769585837 1107 12-13 -0.06971736475868795 1107 14-15 -0.023038060288222084 1107 16-17 -0.007225919468915265 1107 18-19 0.04170890273268668 1107 20-21 -0.064732839177573 1107 22-23 -0.0070332273901740905 1107 24-25 -0.05315974959695069 1107 26-27 0.044713374921173 1107 28-29 0.06797515398251619 1107 30-31 -0.12662941941233186 1107 32-33 -0.0907604906818662 1107 34-35 -0.09747085882711559 1107 36-37 0.050348534866614614 1107 38-39 0.2378006551510481 1107 40-41 0.36901939658545047 1107 42-43 0.13322839338545833 1107 44-45 0.3724841680782376 1107 46-47 0.5314206169455815 1107 48-49 0.40753455500356495 1107 50-51 0.4581122537323239 1107 52-53 0.3123481970532751 1107 54-55 0.47592455005337087 1107 56-57 0.2767190869246505 1107 58-59 0.22285435928357344 1107 60-61 0.5327391764318854 1107 62-63 0.20989487923360528 1107 64-65 0.3457552968429276 1107 66-67 0.44284628462447273 1107 68-69 0.47019945040069544 1107 70-71 0.5086879146029979 1107 72-73 0.5048793482058436 1107 74-75 0.34685813131150667 1107 76-77 0.4058548100037953 1107 78-79 0.3053647463121685 1107 80-81 0.5661411478841458 1107 82-83 0.6097326449196423 1107 84-85 0.6780751722702227 1107 86-87 0.7017240006522876 1107 88-89 0.23732221531954778 1107 90-91 0.5334596560493559 1107 92-93 0.5157526904096663 1107 94-95 0.6047540732748082 1107 96-97 0.5788241462907422 1107 98-99 0.9151232959536735 1107 100-101 1.1536351081386726 1108 1 0.03533460768288421 1108 2 0.13250872798113988 1108 3 0.07619422159437761 1108 4 -0.010216458703624198 1108 5 -0.007041866858507717 1108 6 0.048556245556135025 1108 7 -0.023875746306252665 1108 8 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2316 52-53 -1.3691309097384021 2316 54-55 -1.0146496499417523 2316 56-57 -1.2662128050969557 2316 58-59 -1.1874653239578024 2316 60-61 -1.3303568316520575 2316 62-63 -1.4106499995916018 2316 64-65 -1.4805172400569369 2316 66-67 -1.8967037366540005 2316 68-69 -1.7289042291811576 2316 70-71 -1.1633499254541455 2316 72-73 -0.9202424640074369 2316 74-75 -0.7828678919767 2316 76-77 -0.7366603239568335 2316 78-79 -0.9750395633748816 2316 80-81 -1.325792811992347 2316 82-83 -0.9739423625325081 2316 84-85 -0.946697748017062 2316 86-87 -1.1152694358300757 2316 88-89 -1.029125948639031 2316 90-91 -1.275585756143677 2316 92-93 -1.0455410218491714 2316 94-95 -1.075666734257208 2316 96-97 -1.0009162188740568 2316 98-99 -1.172129212937353 2316 100-101 -1.6888521857085692 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 23.0 5 50.0 6 131.0 7 330.0 8 733.0 9 1358.0 10 2289.0 11 3835.0 12 5296.0 13 7334.0 14 9635.0 15 13470.0 16 17780.0 17 22952.0 18 18338.0 19 10354.0 20 8595.0 21 8719.0 22 9132.0 23 10201.0 24 11728.0 25 13289.0 26 16092.0 27 19270.0 28 23876.0 29 28927.0 30 35268.0 31 42689.0 32 53278.0 33 66343.0 34 88021.0 35 115983.0 36 155356.0 37 188397.0 38 106761.0 39 5636.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.38678370084921 19.46959965746093 15.319257118390068 19.82435952329979 2 16.048050608123233 21.99837524667804 38.94654989606751 23.00702424913122 3 19.562203002765212 27.962794032278342 30.383446893394435 22.09155607156201 4 12.6929833165399 17.504079467127966 36.79420760252169 33.008729613810445 5 12.475211151058609 39.996986103977626 32.04823660332135 15.479566141642412 6 29.417749465882466 37.13570330528696 17.125110122731115 16.321437106099463 7 26.86198646962889 36.11032593624301 19.813602953961606 17.214084640166494 8 28.186757285258636 32.31194219765541 19.128078418014816 20.373222099071135 9 28.917151674737994 15.712923967795522 20.06591413506869 35.304010222397785 10-11 25.381552102950405 27.446917979227305 25.07659575985847 22.09493415796382 12-13 28.00592439618017 25.360455012173816 26.594956862210083 20.038663729435928 14-15 24.30185469125559 25.489955669138308 27.564629295820115 22.64356034378599 16-17 20.949264232164232 29.00247225126455 27.966088470481232 22.082175046089986 18-19 20.936407969133562 25.852870812330526 33.039197415551094 20.171523802984815 20-21 22.653206441148594 24.189885303042328 33.14579790758081 20.01111034822827 22-23 21.990178891482724 24.503772753780336 33.073256160262225 20.432792194474715 24-25 21.616255828852974 24.901903174275514 33.308514684095 20.173326312776506 26-27 20.97351291895596 25.04437400880213 33.57764860778637 20.404464464455536 28-29 20.754231578815958 25.40984885673916 33.51368511070121 20.322234453743672 30-31 21.08027398078675 25.107147745516915 33.23049170928575 20.582086564410588 32-33 20.709093884073052 25.379601033818457 33.03555545449803 20.87574962761046 34-35 21.31937031775714 25.21216952873708 32.95108485800225 20.51737529550353 36-37 21.593217557871384 25.08025925522978 32.67488045829818 20.651642728600653 38-39 21.385280149956685 25.20986222016327 32.735518982905596 20.66933864697445 40-41 21.251579968121984 25.444809790786582 32.524092423955324 20.779517817136107 42-43 21.083727941369887 25.842698633001376 31.709945370881027 21.363628054747714 44-45 21.295824030017904 25.69609902769951 31.447242176298122 21.560834765984463 46-47 21.94718302166831 26.260068757570938 30.37098860297117 21.421759617789586 48-49 21.948187785171825 26.693063497739132 29.432496368615208 21.926252348473838 50-51 22.034432562691293 27.047373196280766 29.294295198032934 21.623899042995003 52-53 22.29210703835263 27.07534050828014 28.735113560720798 21.89743889264643 54-55 21.550623726253303 27.0824128323668 28.95098335139782 22.41598008998208 56-57 22.265910272403588 27.238397055269864 28.09790086324892 22.39779180907762 58-59 22.452466278544087 26.618839430852624 28.437305778948467 22.491388511654822 60-61 22.187395920507456 26.95631548230242 29.079615154999583 21.776673442190546 62-63 22.471146137911784 26.871343386595832 29.031849598770137 21.625660876722247 64-65 22.143732658094198 27.146717817903916 28.911908134660802 21.797641389341084 66-67 22.176562171185214 27.226310176449818 28.41147012979601 22.185657522568956 68-69 22.410729771218357 27.188335003060793 28.56384266485828 21.83709256086257 70-71 22.399488531413724 27.238109759767593 28.279370989940496 22.083030718878195 72-73 22.21214609844462 27.40986550603988 28.215063715878273 22.162924679637225 74-75 21.953261789888057 28.109449076235276 27.910281327232124 22.027007806644544 76-77 21.879373509467847 27.487051402487932 28.256766776330895 22.376808311713326 78-79 21.82032840797171 27.352543447467788 28.42206073349411 22.405067411066394 80-81 21.861971091232625 27.942139662545273 27.92345806605795 22.27243118016416 82-83 22.006584461953864 27.717963168388877 27.60926247605731 22.666189893599945 84-85 21.67587816918489 27.697151122144703 27.556704689751598 23.070266018918804 86-87 21.571569206185583 27.832147067268508 27.322046714688003 23.274237011857903 88-89 21.611028945229368 27.533749815516913 27.362932966760052 23.49228827249367 90-91 21.659966325882696 27.305285837607997 27.587895153638158 23.446852682871146 92-93 21.724510595952083 27.41741613209852 27.643949972625116 23.214123299324278 94-95 21.221486767613086 27.242336504516906 27.82555309941656 23.710623628453444 96-97 21.1622958077473 27.02455679486099 27.924231014617977 23.88891638277373 98-99 21.072354837113224 27.19003315830516 27.47613890552179 24.261473099059824 100-101 21.129070875353847 27.244005737335435 27.49413358789007 24.13278979942065 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1241.0 1 909.5 2 815.0 3 1025.0 4 893.5 5 844.0 6 1295.0 7 2520.5 8 3775.5 9 4374.0 10 3912.5 11 3064.5 12 2899.5 13 2955.5 14 2990.5 15 3084.0 16 3257.5 17 3414.5 18 3518.5 19 3695.5 20 3918.5 21 4286.5 22 4696.5 23 5192.5 24 5831.0 25 6504.5 26 7508.5 27 8931.0 28 10306.5 29 11975.5 30 14135.5 31 16054.5 32 18884.5 33 21184.0 34 22426.5 35 23661.5 36 26097.5 37 28617.0 38 29582.5 39 29762.5 40 29415.5 41 29600.5 42 29688.0 43 30693.5 44 32274.0 45 34029.0 46 34933.5 47 37802.5 48 46978.5 49 46779.5 50 38683.5 51 34546.0 52 31977.5 53 32365.5 54 32904.5 55 31742.0 56 29743.5 57 27683.0 58 26764.5 59 24925.0 60 21836.5 61 19480.0 62 16953.0 63 12537.0 64 8676.5 65 6809.5 66 4857.5 67 3742.0 68 3020.5 69 2161.0 70 1573.0 71 1264.0 72 1142.0 73 1097.0 74 901.0 75 752.0 76 611.0 77 377.0 78 230.5 79 148.0 80 98.5 81 81.5 82 74.5 83 43.5 84 22.0 85 16.0 86 9.0 87 5.0 88 4.0 89 1.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.038966470110399054 2 0.006776777410504183 3 0.003923397448186632 4 6.241768667569642E-4 5 4.4584061911211734E-4 6 8.916812382242346E-5 7 0.0 8 0.0 9 0.0016050262288036224 10-11 4.4584061911211734E-4 12-13 0.0011146015477802934 14-15 0.0020954509098269516 16-17 0.0026304596527614924 18-19 0.0 20-21 0.0 22-23 0.001337521857336352 24-25 7.579290524905994E-4 26-27 0.003878813386275421 28-29 0.00512716711978935 30-31 0.00102543342395787 32-33 3.120884333784821E-4 34-35 0.007668458648728418 36-37 0.009897661744289006 38-39 0.00771304271063963 40-41 0.0012929377954251401 42-43 4.90424681023329E-4 44-45 0.001159185609691505 46-47 4.458406191121173E-5 48-49 3.5667249528969385E-4 50-51 8.470971763130229E-4 52-53 0.0025412915289390685 54-55 0.002808795900406339 56-57 0.0014266899811587754 58-59 8.916812382242347E-4 60-61 0.0034329727671633036 62-63 0.004458406191121173 64-65 0.008604723948863864 66-67 0.0022292030955605867 68-69 0.003254636519518456 70-71 4.458406191121173E-5 72-73 0.0012483537335139284 74-75 0.00539467149125662 76-77 0.0020954509098269516 78-79 0.003254636519518456 80-81 0.004681326500677232 82-83 0.00436923806729875 84-85 0.0047259105625884435 86-87 0.0027642118384951274 88-89 0.008961396444153558 90-91 0.01257270545896171 92-93 9.80849362046658E-4 94-95 0.0021400349717381633 96-97 0.0013821059192475637 98-99 0.0034329727671633036 100-101 0.004502990253032385 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1121477.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.521238887225344 #Duplication Level Percentage of deduplicated Percentage of total 1 70.45226891438749 31.366222944844857 2 14.626437202811019 13.023742095506988 3 5.903449272526779 7.8848662596234425 4 2.9025226097708723 5.16895609940727 5 1.6629544426411895 3.7018395999700533 6 0.9938460076271585 2.654835331361034 7 0.6854771010051383 2.1362802835900716 8 0.4763177488151238 1.6965005024978816 9 0.32833459372022555 1.3156076593762416 >10 1.7554965821898705 14.059623739599699 >50 0.13161100321301653 4.050061486158134 >100 0.07142575564040649 6.346131305476625 >500 0.00704197546557392 2.2197037013408383 >1k 0.002213192289180375 1.7627743800922664 >5k 2.0119929901639774E-4 0.6056261793690642 >10k+ 4.023985980327955E-4 2.007228431785533 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12238 1.0912394993388184 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10170 0.9068398192740466 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6761 0.602865685163405 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3422 0.30513331972033314 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2464 0.21971025709845143 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2266 0.20205496858161157 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1992 0.17762290265426756 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1674 0.1492674392787369 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1641 0.1463248911925969 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1392 0.12412202836081347 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1351 0.1204661352840941 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1231 0.10976596042540328 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1194 0.10646673984397362 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.458406191121173E-5 0.0 24-25 0.0 0.0 0.0 1.3375218573363518E-4 0.0 26-27 0.0 0.0 0.0 4.012565572009056E-4 0.0 28-29 0.0 0.0 0.0 9.362653001354464E-4 0.0 30-31 0.0 0.0 0.0 0.0020508668479157395 0.0 32-33 0.0 0.0 0.0 0.003700477138630574 0.0 34-35 0.0 0.0 0.0 0.00463674243876602 0.0 36-37 0.0 0.0 0.0 0.005974264296102372 0.0 38-39 0.0 0.0 0.0 0.008292635515485383 0.0 40-41 0.0 0.0 0.0 0.012394369211316862 0.0 42-43 0.0 0.0 0.0 0.01783362476448469 0.0 44-45 0.0 0.0 0.0 0.031966772390338814 0.0 46-47 0.0 0.0 0.0 0.05916305015617797 0.0 48-49 0.0 0.0 0.0 0.08252509859765292 0.0 50-51 0.0 0.0 0.0 0.11319893319256659 0.0 52-53 0.0 0.0 0.0 0.149713279897849 0.0 54-55 0.0 0.0 0.0 0.19202355465158893 0.0 56-57 0.0 0.0 0.0 0.23821264279160426 0.0 58-59 0.0 0.0 0.0 0.3085217084255852 0.0 60-61 0.0 0.0 0.0 0.3931868419949763 0.0 62-63 0.0 0.0 0.0 0.4947493350287166 0.0 64-65 0.0 0.0 0.0 0.5786119554837059 0.0 66-67 0.0 0.0 0.0 0.699702267634557 0.0 68-69 0.0 0.0 0.0 0.8532497768567702 0.0 70-71 0.0 0.0 0.0 0.9769705486603828 0.0 72-73 0.0 0.0 0.0 1.1534342657049588 0.0 74-75 0.0 0.0 0.0 1.303370465912364 0.0 76-77 0.0 0.0 0.0 1.4432752521897463 0.0 78-79 0.0 0.0 0.0 1.626025321963803 0.0 80-81 0.0 0.0 0.0 1.8185393012964153 0.0 82-83 0.0 0.0 0.0 2.050242671048983 0.0 84-85 0.0 0.0 0.0 2.359611476650881 0.0 86-87 0.0 0.0 0.0 2.746868638411666 0.0 88-89 0.0 0.0 0.0 3.1912379834807134 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11600 0.0 27.529955 1 GTATCAA 16250 0.0 26.963223 1 GTACATG 11395 0.0 24.68889 1 TACATGG 11585 0.0 23.945139 2 ACATGGG 11395 0.0 23.258497 3 GAGTACT 10225 0.0 23.182333 12-13 TAAGGTG 515 0.0 22.133314 5 ATCAACG 19920 0.0 21.983833 3 TATCAAC 19910 0.0 21.972977 2 TCAACGC 20015 0.0 21.878511 4 GTACTTT 10830 0.0 21.798595 14-15 ATCACGC 110 0.0028785462 21.589327 3 CAACGCA 20300 0.0 21.571352 5 CATGGGA 7440 0.0 21.449087 4 AACGCAG 20595 0.0 21.333452 6 AGTACTT 10405 0.0 21.206234 12-13 GGGACCG 180 5.1738407E-6 21.108625 5 GTAAGGT 440 0.0 20.508945 4 ATGGGAG 2505 0.0 20.47663 5 ATGGGAT 2495 0.0 20.368343 5 >>END_MODULE