FastQCFastQC Report
Thu 26 May 2016
SRR936408_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936408_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144214
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112460.9828581017187344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84340.7370998781696431No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52450.4583932725871209No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29580.25851807441614943No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20210.17662779864605746No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19780.17286976037699242No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18520.16185783428624367No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16380.14315503917973388No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14260.124627036550855No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14000.12235473434165287No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13730.11999503589363528No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG12320.10767216622065454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA157750.030.0368981
GTACATG121450.026.7662721
GGTATCA107900.026.6038681
TACATGG122550.026.1724152
GAGTACT86100.024.97998812-13
ACATGGG123100.024.9404223
ATCAACG189250.024.8615743
TCAACGC189800.024.814574
TATCAAC192700.024.486172
CAACGCA192000.024.4828725
AACGCAG194750.024.162626
AGTACTT88550.023.10794812-13
ATGGGAG30750.023.0307715
GTACTTT91550.022.99668114-15
CATGGGA79700.022.8982454
ACGCAGA211950.022.1345167
CGCAGAG213050.021.9561848
CATGGGG39850.021.824894
TAGCGCG2205.607035E-821.6026294
GCAGAGT217600.021.235859
Produced by FastQC (version 0.11.5)