FastQCFastQC Report
Thu 26 May 2016
SRR936408_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936408_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144214
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120561.053649055159262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102990.9000938635604878No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69950.611336690514187No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34110.2981085705995557No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24900.21761663465051118No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23810.20809044461962534No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20590.1799488557210452No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16830.14708786992642986No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16550.14464077523959679No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14850.12978341464096751No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13160.11501345028115369No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13010.1137025066989217No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG12370.10810914741473186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116400.027.9199871
GTATCAA163950.027.5311491
GCGAACT350.00838747927.14670456-57
GTCGATC700.00839828127.13848
GTACATG111550.026.152361
TACATGG113200.025.3436852
ACATGGG113950.023.9638633
GAGTACT109350.023.54082912-13
AGTACTT110750.023.41478512-13
CGACAGC2554.9112714E-1022.3492728
ATCAACG202450.022.3082773
TATCAAC204850.022.073962
TCAACGC204500.022.0614264
GTACTTT115800.022.00307814-15
TAGCGCT652.932545E-421.93433492-93
CAACGCA206450.021.8300445
TAAGGTG5250.021.709775
ATGGGAT25850.021.4945645
AACGCAG210250.021.4354956
CATGGGA73650.021.3431514
Produced by FastQC (version 0.11.5)