##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936408_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1144214 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.844314962061294 31.0 31.0 34.0 30.0 34.0 2 31.97274460896301 31.0 31.0 34.0 30.0 34.0 3 32.021574635513986 33.0 31.0 34.0 30.0 34.0 4 35.77489088579584 37.0 35.0 37.0 35.0 37.0 5 35.622244615080746 37.0 35.0 37.0 33.0 37.0 6 35.73452518497414 37.0 35.0 37.0 33.0 37.0 7 35.67420430094371 37.0 35.0 37.0 33.0 37.0 8 35.65125579655554 37.0 35.0 37.0 33.0 37.0 9 37.23336019311073 39.0 37.0 39.0 33.0 39.0 10-11 37.123457237894314 39.0 37.0 39.0 33.0 39.0 12-13 37.01635445816954 39.0 37.0 39.0 33.0 39.0 14-15 38.015368191614506 40.0 37.0 41.0 33.0 41.0 16-17 37.94634307917924 40.0 37.0 41.0 33.0 41.0 18-19 38.18050207391275 40.0 37.5 41.0 33.5 41.0 20-21 38.22642355363595 40.0 37.5 41.0 33.0 41.0 22-23 38.136219273667336 40.0 37.0 41.0 33.0 41.0 24-25 37.97230850173132 40.0 37.0 41.0 33.0 41.0 26-27 37.73113858072004 40.0 37.0 41.0 33.0 41.0 28-29 37.60288416327715 39.0 36.5 41.0 32.0 41.0 30-31 37.436693223470435 39.0 36.0 41.0 32.0 41.0 32-33 37.140353989725696 39.0 35.0 41.0 32.0 41.0 34-35 36.892722427797594 39.0 35.0 41.0 31.0 41.0 36-37 36.60822145158161 39.0 35.0 41.0 31.0 41.0 38-39 36.35816726591354 39.0 35.0 40.5 30.0 41.0 40-41 36.06456440840612 38.5 35.0 40.0 30.0 41.0 42-43 35.650266908113345 38.0 35.0 40.0 29.0 41.0 44-45 35.16266231666454 38.0 34.5 40.0 26.5 41.0 46-47 34.50412903530284 38.0 34.0 40.0 23.5 41.0 48-49 33.897356613360785 38.0 33.0 40.0 20.5 41.0 50-51 33.51727037075233 38.0 33.0 40.0 18.0 41.0 52-53 33.129144111154034 37.5 33.0 40.0 14.0 41.0 54-55 32.91123295117871 37.0 33.0 40.0 12.5 41.0 56-57 32.59501063612226 37.0 32.0 40.0 8.5 41.0 58-59 32.22158398691154 36.0 31.5 39.5 7.5 41.0 60-61 31.783883521788756 36.0 31.0 39.0 6.0 40.5 62-63 31.440830998397153 35.0 31.0 39.0 2.0 40.0 64-65 31.00966165420105 35.0 30.0 38.5 2.0 40.0 66-67 30.587751504526253 35.0 29.5 38.0 2.0 40.0 68-69 30.232418061656297 35.0 29.0 37.0 2.0 40.0 70-71 29.873276764661156 34.0 29.0 37.0 2.0 39.0 72-73 29.435186075332062 34.0 29.0 36.0 2.0 39.0 74-75 28.947293513276364 34.0 28.0 36.0 2.0 38.5 76-77 27.48933197810899 32.0 26.0 34.5 2.0 36.5 78-79 28.240889816065874 33.5 27.0 35.0 2.0 37.0 80-81 28.036050511530185 34.0 27.0 35.0 2.0 37.0 82-83 27.739393592457354 33.0 26.5 35.0 2.0 36.0 84-85 27.363351610800077 33.0 26.0 35.0 2.0 36.0 86-87 26.991996689430472 33.0 25.5 35.0 2.0 35.5 88-89 26.52747300767164 33.0 25.0 35.0 2.0 35.0 90-91 26.04604601936351 32.5 24.0 35.0 2.0 35.0 92-93 25.44918389392194 32.0 21.5 34.0 2.0 35.0 94-95 24.926057538187788 32.0 18.5 34.0 2.0 35.0 96-97 24.235953239516384 31.0 8.5 34.0 2.0 35.0 98-99 23.411001788127045 31.0 2.0 34.0 2.0 35.0 100-101 20.4643047541806 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25707490667759814 1101 2 0.2597466847961556 1101 3 0.2717353815674812 1101 4 0.30893296999580144 1101 5 0.2426564988142843 1101 6 0.25304219659997074 1101 7 0.15696409754383467 1101 8 0.17807779249218925 1101 9 0.20003293109777331 1101 10-11 0.37796734204594173 1101 12-13 0.33700703677066457 1101 14-15 0.5939500375028715 1101 16-17 0.5455750014130274 1101 18-19 0.4919902246188741 1101 20-21 0.4832591923659635 1101 22-23 0.4226039522366989 1101 24-25 0.2890875803369397 1101 26-27 0.34633631622119054 1101 28-29 0.3277940466674565 1101 30-31 0.4107379686162034 1101 32-33 0.45614015135247143 1101 34-35 0.542274917556476 1101 36-37 0.39463272173296104 1101 38-39 0.3410697367282367 1101 40-41 0.3337622313317681 1101 42-43 0.4686961419912379 1101 44-45 0.41565853160756916 1101 46-47 0.27440639552354895 1101 48-49 0.3662219267651139 1101 50-51 0.25360993166092527 1101 52-53 0.35698989367381273 1101 54-55 0.336800415443129 1101 56-57 0.30589731960820643 1101 58-59 0.2514265936290414 1101 60-61 0.3300823144504683 1101 62-63 0.06184866805638478 1101 64-65 0.16438987066688782 1101 66-67 0.15470129282663692 1101 68-69 0.24931223444199446 1101 70-71 0.251432493414395 1101 72-73 0.5690637000243157 1101 74-75 0.2841362939893841 1101 76-77 0.21609967760490534 1101 78-79 0.1570266362984647 1101 80-81 0.1496222224152426 1101 82-83 0.10592238736202475 1101 84-85 0.08307698711563916 1101 86-87 0.10456835578580836 1101 88-89 -0.031399250025511805 1101 90-91 0.04324984853155911 1101 92-93 0.25551332486440614 1101 94-95 0.21195869951307955 1101 96-97 0.41033030784210567 1101 98-99 0.3012098818726514 1101 100-101 1.0667301611874223 1102 1 0.25960755815015446 1102 2 0.18097635871390594 1102 3 0.223450800198286 1102 4 0.17823247698019173 1102 5 0.2145594742067516 1102 6 0.17241833183329192 1102 7 0.1864132661205815 1102 8 0.26427173358979417 1102 9 0.41336962508211883 1102 10-11 0.28420619243299683 1102 12-13 0.28424031099979175 1102 14-15 0.43736453551351673 1102 16-17 0.37860008736640793 1102 18-19 0.1919470452087637 1102 20-21 0.14583473944030345 1102 22-23 -0.0136290462929054 1102 24-25 -0.028145937156466516 1102 26-27 0.08282028240204653 1102 28-29 0.2286145230329808 1102 30-31 0.0906683950314644 1102 32-33 0.046223280163609104 1102 34-35 -0.015393583503076513 1102 36-37 0.13925522962553316 1102 38-39 0.06733998172240518 1102 40-41 0.07829959672552889 1102 42-43 0.12542422387939922 1102 44-45 0.1549981501691704 1102 46-47 0.08420379692042701 1102 48-49 0.02098159516886966 1102 50-51 -0.09285449228925557 1102 52-53 0.020996983476173625 1102 54-55 0.08982601495443987 1102 56-57 -0.20616554799115505 1102 58-59 -0.09528109460345746 1102 60-61 0.1011131873908333 1102 62-63 0.2628697698814051 1102 64-65 0.12542737931628523 1102 66-67 0.004770420426861932 1102 68-69 0.06916996538088682 1102 70-71 0.0015105407049702535 1102 72-73 -0.048559986348557516 1102 74-75 -0.09110967322940411 1102 76-77 -0.06254131296725518 1102 78-79 -0.16955566271117917 1102 80-81 -0.0500697104005674 1102 82-83 -0.010596473728661238 1102 84-85 0.011458968800539537 1102 86-87 0.1469497239041324 1102 88-89 0.13548646711759105 1102 90-91 -0.033603757726275774 1102 92-93 -0.19049720847901952 1102 94-95 -0.116099064942226 1102 96-97 0.0580065933586944 1102 98-99 -0.13537213332866216 1102 100-101 0.40465330589182713 1103 1 0.1813579568742405 1103 2 0.18002938901693 1103 3 0.15484533767834563 1103 4 0.0868249809674353 1103 5 0.1454805268383268 1103 6 0.10426143390665743 1103 7 0.04454225336140638 1103 8 0.23705881492951164 1103 9 0.2570947048269332 1103 10-11 0.09288093326234304 1103 12-13 0.21506168261063152 1103 14-15 0.19091567187715697 1103 16-17 0.2904197643998998 1103 18-19 0.29744694552773865 1103 20-21 0.23796492285337933 1103 22-23 0.1351199568968866 1103 24-25 0.3404993977717652 1103 26-27 0.2830296125455902 1103 28-29 0.27140260118289206 1103 30-31 0.28080994208090004 1103 32-33 0.356044352731395 1103 34-35 0.36334445477443467 1103 36-37 0.23948449597322252 1103 38-39 0.32643587486435166 1103 40-41 0.38940406243525416 1103 42-43 0.3973166680580249 1103 44-45 0.5597030943478032 1103 46-47 0.5978476366333467 1103 48-49 0.3654667426967748 1103 50-51 0.29318518075699984 1103 52-53 0.3960592841141306 1103 54-55 0.40169054844726304 1103 56-57 0.06405723909018235 1103 58-59 0.3240520393678352 1103 60-61 0.2884387456045481 1103 62-63 0.3466641279356324 1103 64-65 0.1807378856958728 1103 66-67 0.021678813568808408 1103 68-69 0.055501205412785026 1103 70-71 0.039302107690616594 1103 72-73 0.014139375692909795 1103 74-75 0.043360023740291354 1103 76-77 -0.06690235762435037 1103 78-79 -0.2775102081657259 1103 80-81 0.1010093366488718 1103 82-83 0.08073626151854896 1103 84-85 0.46006094248475193 1103 86-87 0.4292712948770223 1103 88-89 0.26443632756416235 1103 90-91 0.3740293164364026 1103 92-93 0.3483410092243311 1103 94-95 0.40412372213911496 1103 96-97 0.42129407342248726 1103 98-99 0.5988159647574633 1103 100-101 0.7303361208838552 1104 1 0.14356568459835373 1104 2 0.10106697440406975 1104 3 0.029811483746776446 1104 4 0.03554379773490979 1104 5 0.1122547737764279 1104 6 -0.03616843579332141 1104 7 0.08734114596168752 1104 8 0.010899505850638036 1104 9 0.10022310902783005 1104 10-11 0.03932401351872983 1104 12-13 0.1145531213274893 1104 14-15 0.30066259493092673 1104 16-17 0.29854846952463276 1104 18-19 0.21910330555301982 1104 20-21 0.2261423776395759 1104 22-23 0.32021632775542486 1104 24-25 0.27853541835494866 1104 26-27 0.2904812390389253 1104 28-29 0.29959164153678586 1104 30-31 0.34489919609071507 1104 32-33 0.2578228126032016 1104 34-35 0.11332000471274739 1104 36-37 0.35465369040011296 1104 38-39 0.21885748420502438 1104 40-41 0.19448628987348826 1104 42-43 0.14599636472679123 1104 44-45 0.14519179465774812 1104 46-47 0.09732290069401728 1104 48-49 0.12264753773755643 1104 50-51 0.07125197080244305 1104 52-53 0.05644416570060429 1104 54-55 0.026382101515181944 1104 56-57 -0.062212283343690444 1104 58-59 -0.1197918112535703 1104 60-61 -0.06775163386041072 1104 62-63 -0.12941806941022094 1104 64-65 -0.12231498662214335 1104 66-67 -0.20860315206568814 1104 68-69 -0.1269338702695606 1104 70-71 -0.008960433310257088 1104 72-73 0.21896318478682986 1104 74-75 0.1127135641092245 1104 76-77 0.06477062844287573 1104 78-79 -0.011743679924020256 1104 80-81 0.11652196040379792 1104 82-83 0.10457131011774479 1104 84-85 0.3832444909786261 1104 86-87 0.46780271132551476 1104 88-89 0.28556870395969725 1104 90-91 0.4367004668682277 1104 92-93 0.238755832732501 1104 94-95 0.2799644281064353 1104 96-97 0.4189073712468101 1104 98-99 0.5501133226461796 1104 100-101 1.38410443962238 1105 1 0.11986272882444737 1105 2 0.08519802510031838 1105 3 0.09441252459713922 1105 4 0.1512756079706037 1105 5 0.29202500304482015 1105 6 0.24693331838113153 1105 7 0.14310098682008743 1105 8 0.1338173027843439 1105 9 0.1659996276043998 1105 10-11 0.16987990396822994 1105 12-13 0.16548295434937188 1105 14-15 0.3473677409111957 1105 16-17 0.25719827763040826 1105 18-19 0.3560778569625853 1105 20-21 0.31096264006247054 1105 22-23 0.26212185752414996 1105 24-25 0.3182077916143413 1105 26-27 0.22871842432234502 1105 28-29 0.05509478619087105 1105 30-31 0.15128193967581893 1105 32-33 0.284065101670258 1105 34-35 0.19832236571501483 1105 36-37 0.12296024896301816 1105 38-39 0.10859239890753969 1105 40-41 0.17241217449583246 1105 42-43 0.23239648930734091 1105 44-45 0.3331552041459602 1105 46-47 0.3766608656105248 1105 48-49 0.281500291570417 1105 50-51 0.30395009405680895 1105 52-53 0.5377872243539272 1105 54-55 0.425642655697672 1105 56-57 0.30062227570146405 1105 58-59 0.21259209776244603 1105 60-61 0.22219577104645438 1105 62-63 0.22881504691184062 1105 64-65 0.29357807504522526 1105 66-67 0.34684021402026843 1105 68-69 0.32707179613588977 1105 70-71 0.397341684559013 1105 72-73 0.33588805468053096 1105 74-75 0.32604393363119755 1105 76-77 0.25161130600029225 1105 78-79 0.3138498358595321 1105 80-81 0.35520421973226846 1105 82-83 0.32720252156308405 1105 84-85 0.6314498780825311 1105 86-87 0.7070036376421491 1105 88-89 0.7859680649820611 1105 90-91 0.7512286682868385 1105 92-93 0.6035231484236228 1105 94-95 0.6984910119873184 1105 96-97 0.9445933177575476 1105 98-99 0.6589819002176647 1105 100-101 1.0543427501494378 1106 1 0.04922606195684409 1106 2 0.09006068509589227 1106 3 -0.0024212206557407967 1106 4 0.03475763421289457 1106 5 0.0470032014493853 1106 6 0.07625294785447778 1106 7 0.029951208854775757 1106 8 0.013257996416676576 1106 9 -0.04207248845644784 1106 10-11 -0.026320640569316822 1106 12-13 0.16408142321428443 1106 14-15 0.25074121128312754 1106 16-17 0.21639438147432344 1106 18-19 0.18608443762848736 1106 20-21 0.02291910719932133 1106 22-23 -0.09605725715023539 1106 24-25 -0.19316269485259596 1106 26-27 -0.14190869806799356 1106 28-29 -0.42760961632485106 1106 30-31 -0.17318256491557804 1106 32-33 -0.26340360249113814 1106 34-35 -0.03723030975266539 1106 36-37 -0.34817649827913755 1106 38-39 0.00109018860754162 1106 40-41 0.04236364836405215 1106 42-43 0.17665674208529225 1106 44-45 -0.07257550093973464 1106 46-47 0.04150529063111463 1106 48-49 -0.19024554402344762 1106 50-51 0.0590664362112463 1106 52-53 -0.12909042549436833 1106 54-55 -0.027863181503683165 1106 56-57 -0.07243240912985272 1106 58-59 -0.014838981064890788 1106 60-61 0.008113146013801753 1106 62-63 0.1858335029168252 1106 64-65 0.1850749504847755 1106 66-67 0.023315086928018758 1106 68-69 -0.22756280108717064 1106 70-71 0.01344522706710194 1106 72-73 0.033821675352864844 1106 74-75 0.08008777794570321 1106 76-77 0.07656308127306133 1106 78-79 -0.15679084973534074 1106 80-81 0.12634900442895614 1106 82-83 -0.12538152007093117 1106 84-85 0.3034488934943482 1106 86-87 0.29759830880979266 1106 88-89 0.3295938611924001 1106 90-91 0.34354008950973736 1106 92-93 0.3633627509714934 1106 94-95 0.25402103188001846 1106 96-97 0.5360457360266828 1106 98-99 0.47660703333800214 1106 100-101 0.8884283389934495 1107 1 -0.007386789728776932 1107 2 0.16301593369060896 1107 3 0.14436985230314292 1107 4 0.19146380877388225 1107 5 0.042202701790721164 1107 6 -0.06707573749129381 1107 7 -0.027358840965845843 1107 8 -0.05484773077550642 1107 9 -0.12115908627479399 1107 10-11 0.03201795806243268 1107 12-13 -0.11528260136194746 1107 14-15 0.1589643364737654 1107 16-17 0.21755547055590796 1107 18-19 0.06928468600532511 1107 20-21 0.013487868365388067 1107 22-23 -0.14308908134010778 1107 24-25 0.09777772375678495 1107 26-27 0.08398894453080885 1107 28-29 0.13948662606770057 1107 30-31 0.15998917822501113 1107 32-33 0.05939731839139739 1107 34-35 0.1856479565479745 1107 36-37 0.3986361805594498 1107 38-39 0.22993912174914755 1107 40-41 0.4147400772571146 1107 42-43 0.32884202248965977 1107 44-45 0.15312423906345884 1107 46-47 0.3298194147033371 1107 48-49 0.5002511370229215 1107 50-51 0.3511161987281284 1107 52-53 0.5683255839178045 1107 54-55 0.49100474302461095 1107 56-57 0.5505567915615401 1107 58-59 0.5223403804178162 1107 60-61 0.39510894830003096 1107 62-63 0.3884721436709597 1107 64-65 0.37149374868948826 1107 66-67 0.5249146482741942 1107 68-69 0.6645131063597773 1107 70-71 0.45329373172235066 1107 72-73 0.6090891161999608 1107 74-75 0.6609845490468409 1107 76-77 0.5123663842492299 1107 78-79 0.7522338717485262 1107 80-81 0.6208939737086645 1107 82-83 0.5345818770269801 1107 84-85 0.8226272890671424 1107 86-87 0.6138593659364666 1107 88-89 0.6545063483014957 1107 90-91 0.8883051119507996 1107 92-93 1.0030901537631927 1107 94-95 0.9612241416742009 1107 96-97 1.0392003760255228 1107 98-99 0.9351328224065192 1107 100-101 1.1675826594431413 1108 1 0.09642222871864803 1108 2 0.13195298005056344 1108 3 0.12233579503410397 1108 4 0.12135389971118116 1108 5 0.15189271429797913 1108 6 0.07089315108706984 1108 7 -0.01463126953376559 1108 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-2.267628160303012 2316 60-61 -1.674552575107672 2316 62-63 -1.55919611700266 2316 64-65 -1.5181545877739886 2316 66-67 -1.4496312175661856 2316 68-69 -1.9812876760889608 2316 70-71 -1.9761609390336687 2316 72-73 -1.7296908459577196 2316 74-75 -1.661642283228204 2316 76-77 -1.5595309574942888 2316 78-79 -1.6995355811861828 2316 80-81 -1.4520945350618817 2316 82-83 -1.8045229363830195 2316 84-85 -1.4672259535708747 2316 86-87 -1.6316308569614684 2316 88-89 -1.7263902937623996 2316 90-91 -1.5511704089071898 2316 92-93 -1.5203933914906465 2316 94-95 -1.7006653264816087 2316 96-97 -2.269385955496361 2316 98-99 -2.9568591777477984 2316 100-101 -4.131412888209599 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 29.0 5 60.0 6 151.0 7 344.0 8 804.0 9 1382.0 10 2364.0 11 3892.0 12 5522.0 13 7448.0 14 9791.0 15 13346.0 16 17868.0 17 22877.0 18 18859.0 19 10448.0 20 8793.0 21 8896.0 22 9325.0 23 10346.0 24 12043.0 25 13690.0 26 16527.0 27 20021.0 28 24071.0 29 29795.0 30 35568.0 31 43716.0 32 53904.0 33 68300.0 34 89420.0 35 118107.0 36 157431.0 37 193143.0 38 109766.0 39 6161.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.290579410528046 19.466106280480396 15.390573029258558 19.852741279733 2 16.04923487872651 22.001945690243666 38.8558537218537 23.092965709176124 3 19.604953680517497 27.84395679120791 30.442287908862625 22.108801619411967 4 12.648071077613105 17.508001125663554 36.86076206024397 32.983165736479364 5 12.545906158286007 39.88884635486312 32.089912269201925 15.475335217648942 6 29.424390891913575 37.142003156752146 17.15728001929709 16.27632593203719 7 26.83947233646853 36.051298096335124 19.898375653505376 17.210853913690972 8 28.139578785087405 32.274819220880005 19.239670201553206 20.34593179247938 9 28.935146747024593 15.713668946543216 20.059971299075173 35.291213007357015 10-11 25.3345091040662 27.407242001933206 25.181172403064462 22.077076490936136 12-13 27.90214144786434 25.39082898350598 26.66228224462016 20.044747324009517 14-15 24.239366690234583 25.44167989994852 27.594822982823974 22.72413042699292 16-17 20.928873850902825 28.97409443255127 27.98861401303166 22.10841770351425 18-19 20.939732458892603 25.818914208904403 32.97410063642831 20.267252695774683 20-21 22.655814384372157 24.240614080932414 33.10425322535819 19.999318309337237 22-23 21.967544504388606 24.599921518108953 33.00329571504606 20.42923826245638 24-25 21.62283453969275 24.90652972258686 33.211488410384774 20.259147327335622 26-27 21.001855887515823 25.018692156460887 33.51963583178239 20.4598161242409 28-29 20.778253714241764 25.46607736390436 33.35882472729307 20.396844194560806 30-31 21.051281513449645 25.139749028573828 33.107759698093155 20.701209759883376 32-33 20.679240566515983 25.4272731086708 32.8702683993581 21.02321792545511 34-35 21.382696584335832 25.216081846939897 32.821979322390746 20.579242246333525 36-37 21.571746321681868 25.08789945857604 32.62824406465625 20.712110155085846 38-39 21.44862292070334 25.253518406358346 32.61855257442556 20.679306098512757 40-41 21.310867216914346 25.45411165063817 32.37157974221561 20.86344139023188 42-43 21.066027004432566 25.89727442882046 31.61446686632076 21.422231700426213 44-45 21.322956824365246 25.74247041786002 31.3351314077157 21.59944135005904 46-47 21.975133093837982 26.269444066940423 30.292695013116017 21.462727826105578 48-49 22.028216035989974 26.6860250435345 29.34773631530994 21.938022605165582 50-51 22.016904180511688 27.078501049628827 29.196767387918694 21.707827381940792 52-53 22.391120530495865 27.111222784229327 28.599189398822332 21.898467286452473 54-55 21.57182860279183 27.0756496264626 28.817527766221872 22.5349940045237 56-57 22.28100492638395 27.235490942656053 28.016973123411336 22.46653100754866 58-59 22.460867497600386 26.742583051120082 28.36356817788439 22.432981273395143 60-61 22.241462956245055 26.96774870299873 28.946795631061594 21.84399270969462 62-63 22.435511665217884 26.927701128477572 28.900668538066288 21.73611866823826 64-65 22.137185049599676 27.1896780577434 28.8901010000354 21.783035892621523 66-67 22.1714240766313 27.25746823052317 28.364811480711076 22.206296212134454 68-69 22.399225661541557 27.160708004046484 28.527293027239498 21.91277330717246 70-71 22.397534375155676 27.25563334798678 28.219396213816832 22.127436063040708 72-73 22.1723820893644 27.46833197286522 28.142725049684763 22.21656088808562 74-75 21.970138922573312 28.18588732636863 27.735484708291438 22.108489042766617 76-77 21.883757586722094 27.473842769513535 28.22664836545441 22.415751278309962 78-79 21.815098036987735 27.45507639569307 28.3420111195699 22.387814447749296 80-81 21.985686657998535 28.009964708324688 27.7505763578554 22.25377227582138 82-83 22.001377601906267 27.695042993242236 27.589319039839445 22.71426036501206 84-85 21.703452574634728 27.683060415029313 27.509002326311272 23.104484684024683 86-87 21.634029802998832 27.777746214314654 27.301293310473618 23.286930672212904 88-89 21.679314295806325 27.54821057067554 27.25986298681059 23.512612146707543 90-91 21.68230558607366 27.32337741201714 27.547567074372996 23.4467499275362 92-93 21.71446030863485 27.428322650357206 27.5724379215929 23.28477911941504 94-95 21.277629936321325 27.292878199232778 27.756305797522092 23.673186066923808 96-97 21.18261921896136 26.992884942783117 27.886835876257287 23.937659961998232 98-99 21.12742438345755 27.155674262896927 27.456780709950152 24.26012064369537 100-101 21.100760185284777 27.253993530598486 27.40737728643673 24.237868997680003 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1256.0 1 932.5 2 872.5 3 1098.5 4 927.5 5 859.0 6 1311.0 7 2539.0 8 3777.0 9 4275.5 10 3857.5 11 3115.0 12 2897.0 13 2959.0 14 3074.0 15 3219.0 16 3325.5 17 3380.0 18 3490.0 19 3688.5 20 3989.5 21 4243.5 22 4670.0 23 5296.0 24 5836.0 25 6618.5 26 7705.5 27 9048.0 28 10441.5 29 12186.0 30 14303.0 31 16337.0 32 19355.5 33 21452.5 34 22820.5 35 24287.5 36 26557.0 37 29043.0 38 30196.5 39 30571.0 40 30016.5 41 30303.0 42 30485.0 43 31505.5 44 33086.0 45 34723.5 46 35695.5 47 38376.0 48 47583.5 49 47487.5 50 39457.5 51 35299.5 52 32620.0 53 33222.0 54 33821.0 55 32365.5 56 30177.0 57 28157.5 58 27506.0 59 25681.0 60 22353.0 61 20045.0 62 17508.5 63 12947.0 64 8934.0 65 6913.0 66 5034.5 67 3882.0 68 3023.0 69 2177.0 70 1583.5 71 1227.5 72 1113.0 73 1139.5 74 965.0 75 757.0 76 609.0 77 402.0 78 247.0 79 174.5 80 144.0 81 99.0 82 68.0 83 46.5 84 25.5 85 16.5 86 9.0 87 8.0 88 5.0 89 1.0 90 1.0 91 1.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021062493554527387 2 0.012847247105873552 3 0.008302642687469303 4 0.0 5 0.002796679642094923 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.001005056746377863 14-15 2.62188716446399E-4 16-17 4.369811940773317E-5 18-19 0.0013983398210474615 20-21 0.0 22-23 8.739623881546635E-5 24-25 0.0 26-27 0.0015294341792706607 28-29 0.003233660836172255 30-31 0.0017479247763093268 32-33 0.008564831403915701 34-35 0.0025781890450562567 36-37 7.865661493391971E-4 38-39 0.0014857360598629276 40-41 1.747924776309327E-4 42-43 0.005986642358859444 44-45 0.0023596984480175913 46-47 2.1849059703866585E-4 48-49 5.680755523005312E-4 50-51 0.0 52-53 1.310943582231995E-4 54-55 0.0015731322986783942 56-57 0.014857360598629276 58-59 0.02447094686833057 60-61 0.02001373868874179 62-63 0.013153133941727683 64-65 0.017872530837762867 66-67 0.0029714721197258553 68-69 0.001005056746377863 70-71 8.739623881546635E-5 72-73 0.0 74-75 0.0013546417016397283 76-77 0.0024033965674253245 78-79 0.009176605075623965 80-81 0.015425436150929808 82-83 0.01721705904664687 84-85 0.029976909913704955 86-87 0.011754794120680222 88-89 0.03980898678044492 90-91 0.04715027084094409 92-93 0.02953992871962762 94-95 0.030239098630151354 96-97 0.050952007229416875 98-99 0.052306648931056604 100-101 0.059167253678070714 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1144214.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.50986196973608 #Duplication Level Percentage of deduplicated Percentage of total 1 70.4646043373601 31.363698128079637 2 14.730530589841711 13.113077665896588 3 5.88677853141646 7.860590996392568 4 2.789415182420982 4.966259389833763 5 1.628544006568438 3.624313447200106 6 1.042788217206837 2.784861576692609 7 0.6740249424755591 2.10005300076225 8 0.47416740065064783 1.6884100442807102 9 0.34387566652795054 1.3775272606719062 >10 1.7554083997755991 14.137960459911659 >50 0.12761883079626954 3.929878812230508 >100 0.07184073030610927 6.314864283206593 >500 0.007458872770306462 2.3329525505978777 >1k 0.002355433506412567 1.8413402223121322 >5k 1.962861255343806E-4 0.6111299513699643 >10k+ 3.925722510687612E-4 1.9530822105611936 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12056 1.053649055159262 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10299 0.9000938635604878 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6995 0.611336690514187 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3411 0.2981085705995557 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2490 0.21761663465051118 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2381 0.20809044461962534 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2059 0.1799488557210452 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1683 0.14708786992642986 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1655 0.14464077523959679 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1485 0.12978341464096751 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1316 0.11501345028115369 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1301 0.1137025066989217 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1237 0.10810914741473186 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.310943582231995E-4 0.0 24-25 0.0 0.0 0.0 3.0588683585413217E-4 0.0 26-27 0.0 0.0 0.0 4.8067931348506486E-4 0.0 28-29 4.3698119407733166E-5 0.0 0.0 8.302642687469301E-4 0.0 30-31 8.739623881546633E-5 0.0 0.0 0.0013546417016397283 0.0 32-33 8.739623881546633E-5 0.0 0.0 0.002403396567425324 0.0 34-35 8.739623881546633E-5 0.0 0.0 0.0035395476720263865 0.0 36-37 8.739623881546633E-5 0.0 0.0 0.005462264925966646 0.0 38-39 8.739623881546633E-5 0.0 0.0 0.008040453971022904 0.0 40-41 8.739623881546633E-5 0.0 0.0 0.012453964031203954 0.0 42-43 1.310943582231995E-4 0.0 0.0 0.018921285703548462 0.0 44-45 1.7479247763093266E-4 0.0 0.0 0.03312317451106174 0.0 46-47 1.7479247763093266E-4 0.0 0.0 0.06108997093201097 0.0 48-49 1.7479247763093266E-4 0.0 0.0 0.08390038926284768 0.0 50-51 1.7479247763093266E-4 0.0 0.0 0.11370250669892171 0.0 52-53 1.7479247763093266E-4 0.0 0.0 0.15180726682246504 0.0 54-55 1.7479247763093266E-4 0.0 0.0 0.1964230467377606 0.0 56-57 1.7479247763093266E-4 0.0 0.0 0.24326743074285057 0.0 58-59 1.7479247763093266E-4 0.0 0.0 0.3115238932577298 0.0 60-61 1.7479247763093266E-4 0.0 0.0 0.39843945275971104 0.0 62-63 1.7479247763093266E-4 0.0 0.0 0.5061990152191811 0.0 64-65 1.7479247763093266E-4 0.0 0.0 0.5883077815863116 0.0 66-67 1.7479247763093266E-4 0.0 0.0 0.7089582892710629 0.0 68-69 1.7479247763093266E-4 0.0 0.0 0.85879914072018 0.0 70-71 1.7479247763093266E-4 0.0 0.0 0.9810227807036096 0.0 72-73 1.7479247763093266E-4 0.0 0.0 1.1534555598865248 0.0 74-75 1.7479247763093266E-4 0.0 0.0 1.2958240329169195 0.0 76-77 1.7479247763093266E-4 0.0 0.0 1.4353958263052191 0.0 78-79 1.7479247763093266E-4 0.0 0.0 1.618665739101252 0.0 80-81 2.62188716446399E-4 0.0 0.0 1.8118988231222481 0.0 82-83 2.62188716446399E-4 0.0 0.0 2.0462081393865135 0.0 84-85 2.62188716446399E-4 0.0 0.0 2.3472007858669794 0.0 86-87 2.62188716446399E-4 0.0 0.0 2.7258012924155794 0.0 88-89 2.62188716446399E-4 0.0 0.0 3.1548294287607037 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11640 0.0 27.919987 1 GTATCAA 16395 0.0 27.531149 1 GCGAACT 35 0.008387479 27.146704 56-57 GTCGATC 70 0.008398281 27.1384 8 GTACATG 11155 0.0 26.15236 1 TACATGG 11320 0.0 25.343685 2 ACATGGG 11395 0.0 23.963863 3 GAGTACT 10935 0.0 23.540829 12-13 AGTACTT 11075 0.0 23.414785 12-13 CGACAGC 255 4.9112714E-10 22.349272 8 ATCAACG 20245 0.0 22.308277 3 TATCAAC 20485 0.0 22.07396 2 TCAACGC 20450 0.0 22.061426 4 GTACTTT 11580 0.0 22.003078 14-15 TAGCGCT 65 2.932545E-4 21.934334 92-93 CAACGCA 20645 0.0 21.830044 5 TAAGGTG 525 0.0 21.70977 5 ATGGGAT 2585 0.0 21.494564 5 AACGCAG 21025 0.0 21.435495 6 CATGGGA 7365 0.0 21.343151 4 >>END_MODULE