FastQCFastQC Report
Thu 26 May 2016
SRR936407_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936407_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2781390
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96920.3484588640931333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74570.26810335839274607No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45600.16394680357662894No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32090.11537396769241279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA159850.028.7052651
GGTATCA113350.027.6160131
GTACATG128600.024.8952221
ATCAACG190200.024.0761493
TCAACGC190500.023.9883614
TATCAAC190950.023.9073832
TACATGG131900.023.6978762
CAACGCA192700.023.5665865
GAGTACT92600.023.3692512-13
AACGCAG197350.023.0839336
ACATGGG130300.022.4572623
GTACTTT100550.021.64084414-15
ACGCAGA216000.020.9823517
CGCAGAG217250.020.9276058
AATTCGC4450.020.29164578-79
AGTACTT101000.019.7323112-13
GCAGAGT224800.019.549889
ATTCGCG4500.019.5361980-81
CATGGGA75700.018.5744654
AGAGTAC176900.018.41020810-11
Produced by FastQC (version 0.11.5)