##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936407_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2781390 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.35696180686635 31.0 31.0 34.0 30.0 34.0 2 31.44944218538213 31.0 31.0 34.0 28.0 34.0 3 31.45981182070835 33.0 31.0 34.0 28.0 34.0 4 35.08675482402683 37.0 35.0 37.0 32.0 37.0 5 35.09494029963435 37.0 35.0 37.0 32.0 37.0 6 35.085767547880735 37.0 35.0 37.0 32.0 37.0 7 34.99010854285088 37.0 35.0 37.0 32.0 37.0 8 34.9426114280989 37.0 35.0 37.0 32.0 37.0 9 36.440766667026196 39.0 37.0 39.0 32.0 39.0 10-11 36.29761540093263 39.0 37.0 39.0 32.0 39.0 12-13 36.19514289617781 39.0 35.0 39.0 32.0 39.0 14-15 37.29340437694822 40.0 37.0 41.0 32.0 41.0 16-17 37.18382751070508 39.0 37.0 41.0 31.0 41.0 18-19 37.145245003397584 39.5 36.5 41.0 31.0 41.0 20-21 37.00634466939192 39.0 36.0 41.0 31.0 41.0 22-23 37.11750869169732 39.0 37.0 41.0 31.0 41.0 24-25 37.16044837293583 40.0 37.0 41.0 31.0 41.0 26-27 37.06872337212688 39.0 36.0 41.0 31.0 41.0 28-29 36.92538011569755 39.0 36.0 41.0 30.5 41.0 30-31 36.711349001758116 39.0 36.0 41.0 30.0 41.0 32-33 36.529968109470445 39.0 35.5 41.0 30.0 41.0 34-35 36.34657940813766 39.0 35.0 40.5 30.0 41.0 36-37 36.14538414246114 39.0 35.0 40.0 29.5 41.0 38-39 35.9411510072302 39.0 35.0 40.0 29.0 41.0 40-41 35.726350853350304 38.0 35.0 40.0 27.5 41.0 42-43 35.484729937189684 38.0 35.0 40.0 27.0 41.0 44-45 35.21731112860836 38.0 34.0 40.0 26.5 41.0 46-47 35.02737713876874 38.0 34.0 40.0 25.5 41.0 48-49 34.86515465288939 38.0 34.0 40.0 24.0 41.0 50-51 33.903345629343605 37.5 33.0 39.5 23.5 40.5 52-53 34.00884431884777 37.5 33.0 39.5 23.5 40.5 54-55 34.43837829286795 38.0 34.0 40.0 24.0 41.0 56-57 34.337222755528714 38.0 34.0 40.0 23.0 41.0 58-59 34.101594166945304 38.0 33.5 40.0 23.0 41.0 60-61 33.80804058402454 37.5 33.0 40.0 22.0 41.0 62-63 33.43627934234321 37.0 33.0 40.0 21.5 41.0 64-65 33.07545364008643 36.0 32.0 39.5 20.5 41.0 66-67 32.621220325089254 36.0 32.0 39.0 18.5 41.0 68-69 32.232837358299264 35.0 31.0 39.0 18.0 40.0 70-71 31.841147771438024 35.0 31.0 38.0 15.5 40.0 72-73 31.443849118606163 35.0 31.0 37.5 13.0 39.5 74-75 31.00524503935083 35.0 30.5 37.0 9.5 39.0 76-77 30.54234968846512 34.5 30.0 36.0 7.0 39.0 78-79 30.04516087280101 34.0 29.5 36.0 4.5 38.5 80-81 29.591027867361284 34.0 29.0 35.5 2.0 37.0 82-83 29.18232502453809 34.0 29.0 35.0 2.0 37.0 84-85 28.810763503140517 34.0 29.0 35.0 2.0 36.0 86-87 28.436263882447264 34.0 28.5 35.0 2.0 36.0 88-89 28.025197652972075 34.0 27.0 35.0 2.0 36.0 90-91 27.615777902415697 33.5 26.5 35.0 2.0 35.0 92-93 27.161466928406302 33.0 25.5 35.0 2.0 35.0 94-95 26.658452248695795 33.0 25.0 35.0 2.0 35.0 96-97 26.055912511370217 33.0 24.5 35.0 2.0 35.0 98-99 25.272318517000492 32.5 20.0 35.0 2.0 35.0 100-101 22.34109527969828 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4781016002577587 1101 2 0.4237907131064915 1101 3 0.44658759473020027 1101 4 0.5431382754074647 1101 5 0.3671129587227995 1101 6 0.4774777554460101 1101 7 0.6032095961269519 1101 8 0.5202937694002685 1101 9 0.606638915709091 1101 10-11 0.6519236691394568 1101 12-13 0.7308256427650051 1101 14-15 0.8465887515544708 1101 16-17 0.9068364692146957 1101 18-19 0.7975649401264491 1101 20-21 0.7409264242042894 1101 22-23 0.8231254839758719 1101 24-25 0.7969486653577036 1101 26-27 0.7011529010845905 1101 28-29 0.8541324432485169 1101 30-31 0.8302206060991679 1101 32-33 0.6991782173662529 1101 34-35 0.5520949266307511 1101 36-37 0.5609305119535435 1101 38-39 0.6013195117308783 1101 40-41 0.538093895720742 1101 42-43 0.7067200796884023 1101 44-45 0.5271201974529234 1101 46-47 0.7406851643189469 1101 48-49 0.7969121856719639 1101 50-51 0.6015676975237412 1101 52-53 0.7250355338981933 1101 54-55 0.6192318964925576 1101 56-57 0.7041528722591579 1101 58-59 0.7120492929458635 1101 60-61 0.6893317931563416 1101 62-63 0.6933942875274965 1101 64-65 0.5751987704144241 1101 66-67 0.8404273859643823 1101 68-69 0.7196857053764489 1101 70-71 0.712443636784144 1101 72-73 0.5990471025683775 1101 74-75 0.6190256246512789 1101 76-77 0.608660520951986 1101 78-79 0.567180520477077 1101 80-81 0.6220034449444647 1101 82-83 0.4154211048680594 1101 84-85 0.4528746079593198 1101 86-87 0.5018761856922218 1101 88-89 0.599203837493274 1101 90-91 0.46734498742361197 1101 92-93 0.41775632105503036 1101 94-95 0.5378714370411828 1101 96-97 0.488288046984934 1101 98-99 0.5340893142975567 1101 100-101 0.9510487847361162 1102 1 0.4639054512759735 1102 2 0.492831407831801 1102 3 0.4440912638158707 1102 4 0.4675288464772578 1102 5 0.4158777562411231 1102 6 0.4403699418152698 1102 7 0.35530824549762485 1102 8 0.38820093646032916 1102 9 0.4542672548024953 1102 10-11 0.5145930614126257 1102 12-13 0.5655841143512959 1102 14-15 0.6003900032142937 1102 16-17 0.47905320565598686 1102 18-19 0.470543392279545 1102 20-21 0.4766305166720102 1102 22-23 0.38229853631698063 1102 24-25 0.41676747677684034 1102 26-27 0.2006999859932037 1102 28-29 0.021648128448852333 1102 30-31 0.3936390329124677 1102 32-33 0.3198457655152609 1102 34-35 0.3304777559861449 1102 36-37 0.14708295429084473 1102 38-39 0.08582588162431648 1102 40-41 0.027307741982106393 1102 42-43 0.12859047924111877 1102 44-45 0.08998707783960214 1102 46-47 0.1559915639708649 1102 48-49 0.1895983058195796 1102 50-51 0.2940976983578807 1102 52-53 0.516180581703388 1102 54-55 0.39804650565320543 1102 56-57 0.2780346747214466 1102 58-59 0.2624462981603699 1102 60-61 0.15353087289780376 1102 62-63 0.22433583916183863 1102 64-65 0.232357883611634 1102 66-67 0.27226473556569175 1102 68-69 0.30327818549800867 1102 70-71 0.19094008943771357 1102 72-73 0.0320556851853695 1102 74-75 0.05036519599385869 1102 76-77 0.09978122090757324 1102 78-79 0.3214828193198791 1102 80-81 0.4591420879125607 1102 82-83 0.30133288674739944 1102 84-85 -0.05956021081827956 1102 86-87 0.09655060083658284 1102 88-89 0.18305061737743955 1102 90-91 0.2870188953211077 1102 92-93 0.20942653844976178 1102 94-95 0.07539372772294684 1102 96-97 0.18139428025988025 1102 98-99 0.28740011459784753 1102 100-101 0.44785966732311877 1103 1 0.21465922012019334 1103 2 0.15983308287367493 1103 3 0.22298573617767659 1103 4 0.4008621798105878 1103 5 0.3522261649513396 1103 6 0.36298300714190646 1103 7 0.4142697195344738 1103 8 0.38418083595782093 1103 9 0.18894062163666803 1103 10-11 0.41023795253490647 1103 12-13 0.09707490162097798 1103 14-15 0.37710692113724065 1103 16-17 0.4643128371467711 1103 18-19 0.5975115664838953 1103 20-21 0.4407846205246102 1103 22-23 0.2052466100188255 1103 24-25 0.3544559189879024 1103 26-27 0.21493784193960863 1103 28-29 0.40690775993964223 1103 30-31 0.3407077096293776 1103 32-33 0.30460288444322714 1103 34-35 0.5401929988672194 1103 36-37 0.50051679013675 1103 38-39 0.3968811580062237 1103 40-41 0.2700213098213027 1103 42-43 0.2322755713684188 1103 44-45 0.3432534094978976 1103 46-47 0.5844672758636662 1103 48-49 0.5956284565733441 1103 50-51 0.6510491221434762 1103 52-53 0.8193631612678729 1103 54-55 0.8255171924203779 1103 56-57 0.7991402023596379 1103 58-59 0.5974546733697466 1103 60-61 0.6175174725627954 1103 62-63 0.5760946331316887 1103 64-65 0.6837816692062759 1103 66-67 0.68365502032281 1103 68-69 0.6560420045934805 1103 70-71 0.5410238415315121 1103 72-73 0.36220643895421034 1103 74-75 0.3121742412199886 1103 76-77 0.4857108691487788 1103 78-79 0.33974077576879225 1103 80-81 0.5139159572592931 1103 82-83 0.30568799562512083 1103 84-85 0.44529741062225625 1103 86-87 0.4447918068667356 1103 88-89 0.33933202441261656 1103 90-91 0.2742885770631496 1103 92-93 0.2719056004263152 1103 94-95 0.5221273960646542 1103 96-97 0.6343256035429619 1103 98-99 0.5813699638440752 1103 100-101 0.5180439219294826 1104 1 0.3397481622843266 1104 2 0.46102893195496364 1104 3 0.3769047369540566 1104 4 0.35399740887043407 1104 5 0.3546485769547658 1104 6 0.40483061530154885 1104 7 0.4546555763795368 1104 8 0.39363899261547175 1104 9 0.43843711371789595 1104 10-11 0.6594765052304723 1104 12-13 0.5666005149735369 1104 14-15 0.45197958667204574 1104 16-17 0.34109772366197433 1104 18-19 0.2757365908701175 1104 20-21 0.18253354625115037 1104 22-23 0.25881695182405906 1104 24-25 0.4158255856574087 1104 26-27 0.23627116389743463 1104 28-29 0.3066483986169004 1104 30-31 0.43951385126000275 1104 32-33 0.3846764270288219 1104 34-35 0.390771585063618 1104 36-37 0.2596779041391315 1104 38-39 0.161923119369348 1104 40-41 0.16053898023334767 1104 42-43 0.4111566775217099 1104 44-45 0.28887398549615995 1104 46-47 0.13927157466383022 1104 48-49 0.5985306301463638 1104 50-51 0.4958094262104993 1104 52-53 0.49737887651644996 1104 54-55 0.43637794781022876 1104 56-57 0.4637191810106742 1104 58-59 0.43391825898315517 1104 60-61 0.3907520665855344 1104 62-63 0.50659177077236 1104 64-65 0.3950175315968991 1104 66-67 0.4619389129039959 1104 68-69 0.484328883242533 1104 70-71 0.5395711906142253 1104 72-73 0.25742958353961853 1104 74-75 0.3606133808911842 1104 76-77 0.32483205515805835 1104 78-79 0.4671415032317974 1104 80-81 0.5065355350577363 1104 82-83 0.3872884142433968 1104 84-85 0.3447038940865461 1104 86-87 0.45320739925994147 1104 88-89 0.28358071551953756 1104 90-91 0.31864135396406823 1104 92-93 -0.07707615043324978 1104 94-95 -0.17839104219341806 1104 96-97 0.059637221053264966 1104 98-99 0.1658227882376906 1104 100-101 0.04841137890877789 1105 1 0.3936407445903711 1105 2 0.27382673774222255 1105 3 0.41997919532122197 1105 4 0.423767353323079 1105 5 0.4064168420688077 1105 6 0.45831155204030694 1105 7 0.5162686180123686 1105 8 0.5411528563860486 1105 9 0.6336880225907109 1105 10-11 0.63803864075863 1105 12-13 0.3711864011475967 1105 14-15 0.5247292037459346 1105 16-17 0.34584214355198384 1105 18-19 0.4677797438744733 1105 20-21 0.47282838782123804 1105 22-23 0.3244793793910503 1105 24-25 0.35707209087996716 1105 26-27 0.1828589693156104 1105 28-29 0.10137687863092282 1105 30-31 0.021234608161897484 1105 32-33 0.0013078767962966253 1105 34-35 0.09956469885265307 1105 36-37 0.23788439127650207 1105 38-39 0.049674904820001586 1105 40-41 0.10307630399507417 1105 42-43 -5.836409067185855E-4 1105 44-45 0.23365725162519624 1105 46-47 0.30965702670118134 1105 48-49 0.35121543812967815 1105 50-51 0.3556396699914117 1105 52-53 0.49159063696156124 1105 54-55 0.371444348581619 1105 56-57 0.24454686656836344 1105 58-59 0.3693788891042189 1105 60-61 0.4009941289425143 1105 62-63 0.3786708634681588 1105 64-65 0.3783483659144551 1105 66-67 0.5407575051199771 1105 68-69 0.42919502057920766 1105 70-71 0.3319766240127606 1105 72-73 0.3147176362450139 1105 74-75 0.29720127858070455 1105 76-77 0.34209833182447724 1105 78-79 0.4138024336961088 1105 80-81 0.5395006288054311 1105 82-83 0.3735365226806877 1105 84-85 0.29174642425928 1105 86-87 0.19669517561263916 1105 88-89 0.16494605470902357 1105 90-91 0.22790505164621067 1105 92-93 0.01904977920062123 1105 94-95 0.18502567368463474 1105 96-97 0.46963684043393883 1105 98-99 0.5528310396033085 1105 100-101 0.45119370617680943 1106 1 0.3417208902732476 1106 2 0.34319925243477556 1106 3 0.40631799937665747 1106 4 0.30265955493999286 1106 5 0.3288357315018686 1106 6 0.46535904334173495 1106 7 0.560144802188276 1106 8 0.5970204205654568 1106 9 0.6395999933664172 1106 10-11 0.6407404988914323 1106 12-13 0.5886836965483155 1106 14-15 0.5664478760800762 1106 16-17 0.6532834692807654 1106 18-19 0.5133370512265998 1106 20-21 0.31931485294651907 1106 22-23 0.5200827837140238 1106 24-25 0.4841303503241363 1106 26-27 0.5273335460823745 1106 28-29 0.494362349633235 1106 30-31 0.7523950411536262 1106 32-33 0.5776735166898632 1106 34-35 0.5031048457050815 1106 36-37 0.32546235409191837 1106 38-39 0.42429822541004114 1106 40-41 0.4022785385788552 1106 42-43 0.5381712794537918 1106 44-45 0.49387864957408567 1106 46-47 0.4206003059547143 1106 48-49 0.37569331797968886 1106 50-51 0.20483199075687963 1106 52-53 0.20708860759838643 1106 54-55 0.3878812994383125 1106 56-57 0.4101063759759853 1106 58-59 0.483889087088194 1106 60-61 0.47298935112169715 1106 62-63 0.5507664116611934 1106 64-65 0.5070372657273268 1106 66-67 0.6207431734849393 1106 68-69 0.780935695193989 1106 70-71 0.5952516607441467 1106 72-73 0.21286845785841635 1106 74-75 0.5214646407630994 1106 76-77 0.4991341275614438 1106 78-79 0.38320619747494433 1106 80-81 0.6679585869060709 1106 82-83 0.48953504977053797 1106 84-85 0.5322617098137954 1106 86-87 0.48464237105283203 1106 88-89 0.49560250481282253 1106 90-91 0.5833362250768133 1106 92-93 0.5418386550741303 1106 94-95 0.5741087779798306 1106 96-97 0.5616118329414661 1106 98-99 0.754183087971775 1106 100-101 0.47411952523180645 1107 1 0.26673244003260876 1107 2 0.22431533429060124 1107 3 0.22703796535553522 1107 4 0.3328993218979832 1107 5 0.3687895011126656 1107 6 0.36820826764354564 1107 7 0.5004264438821551 1107 8 0.5137564529706182 1107 9 0.47723724963178427 1107 10-11 0.46652220429654534 1107 12-13 0.38273463552192055 1107 14-15 0.25845890248921677 1107 16-17 0.3165825105219753 1107 18-19 0.305789051143293 1107 20-21 0.38169279108102216 1107 22-23 0.38236312834137465 1107 24-25 0.44388976822074255 1107 26-27 0.3615588925807529 1107 28-29 0.30998592130926284 1107 30-31 0.3166424951119069 1107 32-33 0.12874791863851698 1107 34-35 0.29042467673708217 1107 36-37 0.34285669478820324 1107 38-39 0.21174234372168144 1107 40-41 0.34597584105221557 1107 42-43 0.42780098397926025 1107 44-45 0.37574303042972446 1107 46-47 0.41960567180607455 1107 48-49 0.4558611886653736 1107 50-51 0.26929134892841233 1107 52-53 0.42706701520288703 1107 54-55 0.49483347078187734 1107 56-57 0.3012519934161517 1107 58-59 0.2939670387263362 1107 60-61 0.3616636111812994 1107 62-63 0.5206612892561395 1107 64-65 0.3464971781529371 1107 66-67 0.3670563760859693 1107 68-69 0.47291273854964544 1107 70-71 0.3160238833657303 1107 72-73 0.25691357436486584 1107 74-75 0.4184699555559064 1107 76-77 0.18155823786047165 1107 78-79 0.2106489295915175 1107 80-81 0.13911772366708774 1107 82-83 0.3047586067560317 1107 84-85 0.037708851777413344 1107 86-87 0.1586603503835171 1107 88-89 0.034365132008538524 1107 90-91 0.13412756554535576 1107 92-93 0.26301884229332373 1107 94-95 0.26628725630491346 1107 96-97 0.21739446098367665 1107 98-99 0.28822274255614744 1107 100-101 0.055275199864279756 1108 1 0.1663998083346634 1108 2 0.13712331351924334 1108 3 0.2057529772808806 1108 4 0.20186799160465796 1108 5 0.11794686281615441 1108 6 0.19110247061154695 1108 7 0.1976959640299043 1108 8 0.1554272053424839 1108 9 0.2172119121156726 1108 10-11 0.22441130166902923 1108 12-13 0.03800925020612311 1108 14-15 0.2224272834317702 1108 16-17 0.28496468185617374 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-1.3921740419834236 2316 74-75 -1.361741337502469 2316 76-77 -1.440986160688901 2316 78-79 -2.213785732306885 2316 80-81 -2.363877416294482 2316 82-83 -2.3861565010674894 2316 84-85 -2.030933446661816 2316 86-87 -1.885166730119895 2316 88-89 -2.4141212483054453 2316 90-91 -2.661434311450421 2316 92-93 -3.3608722548675622 2316 94-95 -3.0629050322740277 2316 96-97 -3.5722799480442333 2316 98-99 -4.465996791190943 2316 100-101 -5.3828346941096825 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8080.0 3 3249.0 4 3104.0 5 3503.0 6 3805.0 7 4352.0 8 5376.0 9 6636.0 10 7982.0 11 9859.0 12 12002.0 13 14087.0 14 16133.0 15 18982.0 16 22890.0 17 28564.0 18 23518.0 19 18592.0 20 18938.0 21 20214.0 22 22295.0 23 25018.0 24 28398.0 25 32792.0 26 38443.0 27 45260.0 28 53520.0 29 63722.0 30 77651.0 31 95752.0 32 119889.0 33 154030.0 34 202595.0 35 275294.0 36 381093.0 37 492291.0 38 372440.0 39 51027.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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26.244860757309098 28.452968850841064 22.701115847114384 44-45 22.53578880626918 26.540442658901615 28.312458889140764 22.611309645688436 46-47 22.746353453493782 26.68965178781853 27.88205213664013 22.68194262204756 48-49 22.8784935239846 26.72313387048904 27.706405295072212 22.69196731045415 50-51 22.73362032174198 26.825002867367274 27.674606487548797 22.76677032334195 52-53 22.99626074137993 26.949376190989828 27.428091180383273 22.626271887246972 54-55 22.664896480991928 26.686969270139983 27.588541493445852 23.059592755422244 56-57 22.78205444872651 27.126533732129754 27.383637338303547 22.70777448084019 58-59 22.92723266974744 26.69227885888515 27.46382667365399 22.91666179771342 60-61 22.847747003399107 26.590109437324315 28.012942065596242 22.549201493680336 62-63 22.74376148601508 26.79266325983422 27.7801077633822 22.683467490768493 64-65 22.539028414404264 26.878327270975355 27.663659396750738 22.91898491786964 66-67 22.53585904107035 27.087152882846294 27.405698143080887 22.971289933002474 68-69 22.455409497785514 26.816167014331437 27.700702445295178 23.02772104258787 70-71 22.594228576680454 26.788144630372678 27.59391292414573 23.02371386880114 72-73 22.673121694853027 26.71521542101367 27.687389124247186 22.924273759886113 74-75 22.457841074607543 27.156749563935197 27.390732049414684 22.99467731204258 76-77 22.649021603077415 27.007308195165475 27.34242822395016 23.001241977806952 78-79 22.705065065432358 26.94584304831672 27.376692781333418 22.972399104917503 80-81 22.48426784725428 27.31347832329021 27.33458166272726 22.867672166728255 82-83 22.60405697425655 27.194497176736558 27.309082374185145 22.892363474821742 84-85 22.53941655054614 27.10596484919315 27.204135389041216 23.15048321121949 86-87 22.740897755924845 26.98838090967331 27.238200072173175 23.03252126222867 88-89 22.72493361565261 26.955695100935856 27.094882867228424 23.22448841618311 90-91 22.59217264246375 26.99924918511858 27.346116668539956 23.062461503877714 92-93 22.76103287178504 26.858183494664857 27.377546693959427 23.003236939590675 94-95 22.497034817899074 26.86851419790498 27.29632385739582 23.338127126800124 96-97 22.551344139913173 26.831896881099155 27.264693481440595 23.352065497547077 98-99 22.533374700425814 26.901577794864185 26.98929401660979 23.575753488100215 100-101 22.480598679594884 26.78767382036014 27.14231232797856 23.589415172066417 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 229.0 1 200.0 2 273.5 3 391.5 4 339.0 5 331.5 6 624.5 7 1274.0 8 2152.5 9 2674.0 10 2490.5 11 2203.0 12 2235.0 13 2416.5 14 2674.5 15 2915.5 16 3086.0 17 3300.5 18 3517.5 19 3769.5 20 4253.5 21 4841.5 22 5707.5 23 6992.0 24 8558.0 25 10664.5 26 13427.0 27 16790.5 28 20016.5 29 23960.5 30 29547.0 31 35166.0 32 41282.5 33 46618.0 34 52186.0 35 57362.0 36 62749.5 37 68076.5 38 70639.5 39 72804.5 40 75790.0 41 82569.0 42 89375.5 43 94977.0 44 99589.0 45 102858.5 46 103962.5 47 106924.5 48 117460.0 49 117418.0 50 107383.0 51 101193.5 52 97383.0 53 96296.0 54 93793.5 55 87496.0 56 80173.5 57 72182.5 58 65614.0 59 58795.0 60 49994.5 61 40983.0 62 33239.0 63 26191.0 64 20133.5 65 16101.5 66 12562.0 67 10172.5 68 7665.5 69 5251.5 70 4056.5 71 3370.5 72 2774.0 73 2379.5 74 1974.5 75 1616.5 76 1149.5 77 647.5 78 382.0 79 268.5 80 180.0 81 124.5 82 97.5 83 59.5 84 40.0 85 29.0 86 19.0 87 18.0 88 13.5 89 9.5 90 6.5 91 3.5 92 4.0 93 3.0 94 2.5 95 2.0 96 2.5 97 2.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012331963514645555 2 0.011325272615490815 3 0.013518420645792213 4 0.01865973488076106 5 0.03084788540981308 6 0.04533704370836165 7 0.005752519423741367 8 0.025167272478868477 9 0.03318484642570801 10-11 0.039512617791823516 12-13 0.030416446453032472 14-15 0.027000888045186043 16-17 0.029409755553877735 18-19 0.02306400756456304 20-21 0.0524198332488432 22-23 0.012457799877039898 24-25 0.002912212958269067 26-27 0.0010606207687523144 28-29 1.797662319919177E-5 30-31 0.0023189843926957384 32-33 0.0018515921895167523 34-35 1.797662319919177E-5 36-37 2.1571947839030126E-4 38-39 1.797662319919177E-5 40-41 0.0012763402471426158 42-43 0.0034155584078464367 44-45 0.0013841999863377665 46-47 9.168077831587803E-4 48-49 4.134623335814108E-4 50-51 0.0033436519150496697 52-53 0.0032357921758545184 54-55 0.004943571379777737 56-57 7.550181743660544E-4 58-59 0.005842402539737326 60-61 0.002588633740683615 62-63 6.651350583700955E-4 64-65 6.831116815692874E-4 66-67 0.01677218944484592 68-69 0.04219113464850308 70-71 0.05157493195848119 72-73 0.014579041414544526 74-75 0.030200726974642173 76-77 0.02676719194359655 78-79 0.05517025659831955 80-81 0.07942072129402925 82-83 0.048986298217797576 84-85 0.018515921895167525 86-87 0.02079895304146488 88-89 0.022075293288607495 90-91 0.03873962299425827 92-93 0.02434034781170566 94-95 0.013069005065812416 96-97 0.0187855712431554 98-99 0.050424428073732916 100-101 0.06974929801286407 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2781390.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.733347698474184 #Duplication Level Percentage of deduplicated Percentage of total 1 76.14892812428069 39.39438975519533 2 13.311555920859275 13.77302701722991 3 4.4268335811075055 6.870447625641485 4 1.9451035597398907 4.025068750622545 5 1.0769403384600342 2.7856864490032716 6 0.6281798841310009 1.9498709017761777 7 0.4178539109742156 1.5131887164517461 8 0.2969614814895044 1.2290249259960428 9 0.22904193698408765 1.0664195547176838 >10 1.3102756212825017 12.79872778455049 >50 0.1285284508287654 4.612313322978807 >100 0.07489976569552341 7.1304839043177335 >500 0.0037080497264290893 1.2959371532177735 >1k 0.0010494480357690736 0.9347179718346282 >5k 1.399264047692098E-4 0.6206961664662939 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9692 0.3484588640931333 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7457 0.26810335839274607 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4560 0.16394680357662894 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3209 0.11537396769241279 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.595324639838354E-5 7 0.0 0.0 0.0 3.595324639838354E-5 3.595324639838354E-5 8 0.0 0.0 0.0 3.595324639838354E-5 3.595324639838354E-5 9 0.0 0.0 0.0 3.595324639838354E-5 3.595324639838354E-5 10-11 0.0 0.0 0.0 3.595324639838354E-5 3.595324639838354E-5 12-13 0.0 0.0 0.0 3.595324639838354E-5 3.595324639838354E-5 14-15 0.0 0.0 0.0 3.595324639838354E-5 3.595324639838354E-5 16-17 0.0 0.0 0.0 5.392986959757531E-5 3.595324639838354E-5 18-19 0.0 0.0 0.0 8.988311599595886E-5 7.190649279676708E-5 20-21 0.0 0.0 0.0 1.4381298559353417E-4 1.0785973919515063E-4 22-23 0.0 0.0 0.0 1.4381298559353417E-4 1.258363623943424E-4 24-25 0.0 0.0 0.0 2.1571947839030126E-4 1.4381298559353417E-4 26-27 0.0 0.0 0.0 3.95485710382219E-4 1.4381298559353417E-4 28-29 0.0 0.0 0.0 8.808545367603967E-4 1.4381298559353417E-4 30-31 0.0 0.0 0.0 0.0016178960879272594 1.4381298559353417E-4 32-33 0.0 0.0 0.0 0.0030560259438626013 1.7976623199191771E-4 34-35 0.0 0.0 0.0 0.004458202553399559 1.7976623199191771E-4 36-37 0.0 0.0 0.0 0.006219911626920352 2.1571947839030126E-4 38-39 0.0 0.0 0.0 0.008556872642815284 2.516727247886848E-4 40-41 0.0 0.0 0.0 0.011199436253096474 2.8762597118706833E-4 42-43 0.0 0.0 0.0 0.014291415443357457 2.8762597118706833E-4 44-45 0.0 0.0 0.0 0.018012576445590157 2.8762597118706833E-4 46-47 0.0 0.0 0.0 0.02331568028935173 3.0560259438626013E-4 48-49 0.0 0.0 0.0 0.030326563337036518 3.95485710382219E-4 50-51 0.0 0.0 0.0 0.036222895746371414 3.95485710382219E-4 52-53 0.0 0.0 0.0 0.04700886966588648 4.4941557997979427E-4 54-55 0.0 0.0 0.0 0.06629778635861924 5.033454495773696E-4 56-57 0.0 0.0 0.0 0.10138815484344159 5.033454495773696E-4 58-59 0.0 0.0 0.0 0.13904918044574835 5.033454495773696E-4 60-61 0.0 0.0 0.0 0.1766922294248559 5.033454495773696E-4 62-63 0.0 0.0 0.0 0.22271238481478683 5.752519423741367E-4 64-65 1.797662319919177E-5 0.0 0.0 0.27800847777550075 5.752519423741367E-4 66-67 3.595324639838354E-5 0.0 0.0 0.35973020683902657 5.752519423741367E-4 68-69 3.595324639838354E-5 0.0 0.0 0.46311376685757843 5.752519423741367E-4 70-71 3.595324639838354E-5 0.0 0.0 0.558317963320498 6.471584351709038E-4 72-73 3.595324639838354E-5 0.0 0.0 0.6574230870176423 6.831116815692873E-4 74-75 3.595324639838354E-5 0.0 0.0 0.7648334106328131 6.831116815692873E-4 76-77 3.595324639838354E-5 0.0 0.0 0.8883328120112606 6.831116815692873E-4 78-79 3.595324639838354E-5 0.0 0.0 1.00244841607973 6.831116815692873E-4 80-81 3.595324639838354E-5 0.0 0.0 1.1283926382132674 7.550181743660544E-4 82-83 3.595324639838354E-5 0.0 0.0 1.2737695900251311 8.269246671628214E-4 84-85 3.595324639838354E-5 0.0 0.0 1.4561783856273303 8.269246671628214E-4 86-87 3.595324639838354E-5 0.0 0.0 1.675888674367852 8.269246671628214E-4 88-89 3.595324639838354E-5 0.0 0.0 1.935830645828165 8.269246671628214E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15985 0.0 28.705265 1 GGTATCA 11335 0.0 27.616013 1 GTACATG 12860 0.0 24.895222 1 ATCAACG 19020 0.0 24.076149 3 TCAACGC 19050 0.0 23.988361 4 TATCAAC 19095 0.0 23.907383 2 TACATGG 13190 0.0 23.697876 2 CAACGCA 19270 0.0 23.566586 5 GAGTACT 9260 0.0 23.36925 12-13 AACGCAG 19735 0.0 23.083933 6 ACATGGG 13030 0.0 22.457262 3 GTACTTT 10055 0.0 21.640844 14-15 ACGCAGA 21600 0.0 20.982351 7 CGCAGAG 21725 0.0 20.927605 8 AATTCGC 445 0.0 20.291645 78-79 AGTACTT 10100 0.0 19.73231 12-13 GCAGAGT 22480 0.0 19.54988 9 ATTCGCG 450 0.0 19.53619 80-81 CATGGGA 7570 0.0 18.574465 4 AGAGTAC 17690 0.0 18.410208 10-11 >>END_MODULE