FastQCFastQC Report
Thu 26 May 2016
SRR936407_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936407_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2781390
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99460.35759098867832273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91640.3294755499947868No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54280.19515422145042588No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38240.13748521422741866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121100.028.6796721
GTATCAA169300.027.0252861
GTACATG129000.025.3027651
TACATGG134550.023.9752752
ACATGGG130500.023.5509723
ATCAACG202800.022.5565873
GAGTACT109800.022.43025212-13
TCAACGC204650.022.3050544
TATCAAC207050.022.1655962
CAACGCA207350.021.9458855
GTAAGGT10450.021.8180854
AACGCAG209650.021.7266086
TAAGGTG11800.021.334655
GTACTTT117050.020.93967614-15
AGTACTT115000.020.36276812-13
GTGTAGC15250.020.2509081
ACGCAGA233400.019.4954327
CGCAGAG237150.019.2071848
CATGGGG60150.019.0315024
CATGGGA75550.018.8615954
Produced by FastQC (version 0.11.5)