##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936407_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2781390 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82620236644268 31.0 31.0 34.0 30.0 34.0 2 31.9477124746979 31.0 31.0 34.0 30.0 34.0 3 32.06748568161962 33.0 31.0 34.0 30.0 34.0 4 35.81735714876375 37.0 35.0 37.0 35.0 37.0 5 35.67252237190757 37.0 35.0 37.0 33.0 37.0 6 35.72215906435272 37.0 35.0 37.0 33.0 37.0 7 35.641735607016635 37.0 35.0 37.0 33.0 37.0 8 35.60545302888124 37.0 35.0 37.0 33.0 37.0 9 37.19622311146585 39.0 37.0 39.0 33.0 39.0 10-11 37.09077026235084 39.0 37.0 39.0 33.0 39.0 12-13 36.9605476038959 39.0 37.0 39.0 33.0 39.0 14-15 38.0252837609972 40.0 37.0 41.0 33.0 41.0 16-17 38.00293522303596 40.0 37.0 41.0 33.0 41.0 18-19 38.14784010872262 40.0 37.5 41.0 33.0 41.0 20-21 38.15878481622498 40.0 37.0 41.0 33.0 41.0 22-23 38.055844559734524 40.0 37.0 41.0 33.0 41.0 24-25 37.94159413099206 40.0 37.0 41.0 33.0 41.0 26-27 37.736457850211586 40.0 37.0 41.0 32.0 41.0 28-29 37.74786114137176 40.0 37.0 41.0 32.5 41.0 30-31 37.62273647348988 39.5 37.0 41.0 32.0 41.0 32-33 37.43498196944693 39.0 36.0 41.0 32.0 41.0 34-35 37.255811302981606 39.0 36.0 41.0 31.0 41.0 36-37 37.076426714700204 39.0 36.0 41.0 31.0 41.0 38-39 36.877925425776326 39.0 35.5 41.0 31.0 41.0 40-41 36.6425664865409 39.0 35.0 40.0 30.0 41.0 42-43 36.37865761363922 39.0 35.0 40.0 30.0 41.0 44-45 36.098398822171646 38.5 35.0 40.0 30.0 41.0 46-47 35.72638375057076 38.0 35.0 40.0 28.5 41.0 48-49 35.33493702788893 38.0 34.5 40.0 27.0 41.0 50-51 35.019970949776905 38.0 34.0 40.0 26.5 41.0 52-53 34.74020130222658 38.0 34.0 40.0 26.0 41.0 54-55 34.389821815710846 38.0 33.0 40.0 25.0 41.0 56-57 34.04197074124808 37.5 33.0 40.0 24.0 41.0 58-59 33.660595062181144 37.0 33.0 39.5 23.0 41.0 60-61 33.275636282578134 36.0 32.0 39.0 22.5 40.0 62-63 32.930477566971916 36.0 32.0 39.0 22.0 40.0 64-65 32.58744368822783 35.5 31.0 39.0 21.5 40.0 66-67 32.11614354693157 35.0 31.0 38.0 20.0 40.0 68-69 31.656726852401135 35.0 30.5 38.0 18.5 40.0 70-71 31.13976698701009 34.5 30.0 37.0 17.0 39.0 72-73 30.560079492627786 34.0 29.0 36.5 13.0 39.0 74-75 29.996483772502238 34.0 29.0 36.0 9.0 39.0 76-77 28.653624267003188 32.0 27.0 34.5 8.0 36.5 78-79 29.428898680156323 33.5 29.0 35.0 7.0 37.0 80-81 29.26985374219365 34.0 29.0 35.0 6.5 37.0 82-83 28.84860753076699 33.5 28.5 35.0 2.0 36.0 84-85 28.37562945146132 33.0 27.5 35.0 2.0 36.0 86-87 27.903800617676772 33.0 26.5 35.0 2.0 35.5 88-89 27.36414382736689 32.5 26.0 34.5 2.0 35.0 90-91 26.745495957057443 32.0 25.0 34.0 2.0 35.0 92-93 26.113515903918547 32.0 24.5 34.0 2.0 35.0 94-95 25.3090950927414 31.0 21.5 34.0 2.0 35.0 96-97 24.410807545867357 31.0 17.5 34.0 2.0 35.0 98-99 23.17871675672955 31.0 2.0 34.0 2.0 35.0 100-101 19.329540805137 25.0 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34075304044574395 1101 2 0.4621141873383898 1101 3 0.36778929483135414 1101 4 0.25017396451885787 1101 5 0.2770272392904616 1101 6 0.2461581701826958 1101 7 0.31277363277912684 1101 8 0.2724831005275661 1101 9 0.3229871139009646 1101 10-11 0.35815189452696927 1101 12-13 0.45153619729393313 1101 14-15 0.5432952963684343 1101 16-17 0.4398303201045195 1101 18-19 0.4596624441689485 1101 20-21 0.3170652837734309 1101 22-23 0.4656197962343427 1101 24-25 0.4299994349572387 1101 26-27 0.32559933863997514 1101 28-29 0.40880740000789473 1101 30-31 0.5059785776264434 1101 32-33 0.4406338653074684 1101 34-35 0.5012696281825129 1101 36-37 0.6335304298913726 1101 38-39 0.5559317918610276 1101 40-41 0.5629632498928459 1101 42-43 0.7909081103993358 1101 44-45 0.7624436382457347 1101 46-47 0.6895213660994699 1101 48-49 0.5566985788542524 1101 50-51 0.46580980431109964 1101 52-53 0.46070426336888204 1101 54-55 0.5609419572761141 1101 56-57 0.6282450436505158 1101 58-59 0.5027830188696356 1101 60-61 0.3598024718263275 1101 62-63 0.3847296998555976 1101 64-65 0.5213478162634217 1101 66-67 0.6180752607351963 1101 68-69 0.5874828215555503 1101 70-71 0.5245193626414455 1101 72-73 0.5382490289473871 1101 74-75 0.6792473638329142 1101 76-77 0.7005587782129474 1101 78-79 0.5987822771230533 1101 80-81 0.6547669611037854 1101 82-83 0.696073644650955 1101 84-85 0.5656981640458589 1101 86-87 0.6412892182847507 1101 88-89 0.49600652600905093 1101 90-91 0.5608459323393298 1101 92-93 0.551984057147525 1101 94-95 0.5672576510979894 1101 96-97 0.6188035435133052 1101 98-99 0.35904811267916514 1101 100-101 0.6357412227092212 1102 1 0.35418302121545153 1102 2 0.4061664360201114 1102 3 0.41687444692650644 1102 4 0.25832204002424675 1102 5 0.1817916400290187 1102 6 0.15822534770331487 1102 7 0.2292457420919547 1102 8 0.18192059756862733 1102 9 0.29366948493063205 1102 10-11 0.31356190780556403 1102 12-13 0.3467927063984533 1102 14-15 0.49673324655002915 1102 16-17 0.4468755845604946 1102 18-19 0.34887860122159253 1102 20-21 0.3063650801983542 1102 22-23 0.34818397423515535 1102 24-25 0.30937996253906874 1102 26-27 0.3716766295359122 1102 28-29 0.45280886764216177 1102 30-31 0.46938736674926673 1102 32-33 0.4129989996377148 1102 34-35 0.5359119965970436 1102 36-37 0.5127790169270483 1102 38-39 0.5402206655700894 1102 40-41 0.5633488702332201 1102 42-43 0.6448733718794628 1102 44-45 0.703030601775815 1102 46-47 0.6235013828273495 1102 48-49 0.6979706638192269 1102 50-51 0.7388448426702396 1102 52-53 0.83250235206269 1102 54-55 0.7766652011311024 1102 56-57 0.8036237316170443 1102 58-59 0.6892851233805217 1102 60-61 0.6456873132609502 1102 62-63 0.5820869436790232 1102 64-65 0.5971746286564823 1102 66-67 0.5525904229076559 1102 68-69 0.5430236481650432 1102 70-71 0.2770845100473842 1102 72-73 0.23802894016791143 1102 74-75 0.4059501915651147 1102 76-77 0.42804084985423785 1102 78-79 0.3995604501776491 1102 80-81 0.4493153535821044 1102 82-83 0.5251431735426095 1102 84-85 0.16875410097591015 1102 86-87 0.5581375356905305 1102 88-89 0.29573345811236607 1102 90-91 0.2944881226538456 1102 92-93 0.620762223438831 1102 94-95 0.5669127795892557 1102 96-97 0.5689393165913472 1102 98-99 0.6676828633729635 1102 100-101 0.9125522067456018 1103 1 0.2365950815169633 1103 2 0.2969537057018563 1103 3 0.24334010856804866 1103 4 0.1192935643123576 1103 5 0.13355043399886313 1103 6 0.15387023882559703 1103 7 0.1984249715726918 1103 8 0.28041305988018905 1103 9 0.11108992044152188 1103 10-11 0.3013341021104168 1103 12-13 0.26036054559443045 1103 14-15 0.3079560271195376 1103 16-17 0.22024241873134542 1103 18-19 0.16680154929529323 1103 20-21 0.1467335894110846 1103 22-23 0.2058054147711701 1103 24-25 0.21976522217056527 1103 26-27 0.23080560776035952 1103 28-29 0.1554889346438344 1103 30-31 0.3739099798145915 1103 32-33 0.3794981621167679 1103 34-35 0.4166490150225002 1103 36-37 0.29133848091364456 1103 38-39 0.09398950979119292 1103 40-41 0.2931646156268499 1103 42-43 0.5115400385461228 1103 44-45 0.38912775420462964 1103 46-47 0.4107710645693885 1103 48-49 0.48071773249594685 1103 50-51 0.45291519442903194 1103 52-53 0.4738758864010464 1103 54-55 0.3684574959384648 1103 56-57 0.4329369644478618 1103 58-59 0.5172583227105108 1103 60-61 0.20381126635977864 1103 62-63 0.46701156679460354 1103 64-65 0.5212952316715587 1103 66-67 0.24991035590597477 1103 68-69 0.3460387235419269 1103 70-71 0.2365484966470497 1103 72-73 0.44673915792335706 1103 74-75 0.3567039604093374 1103 76-77 0.22670081635340367 1103 78-79 0.1586894284021021 1103 80-81 0.42686979244307466 1103 82-83 0.24189359230307872 1103 84-85 0.2948847542422435 1103 86-87 0.3804155926419561 1103 88-89 0.3355994547622849 1103 90-91 0.45780470556841735 1103 92-93 0.34421280970348533 1103 94-95 0.31682902749545505 1103 96-97 0.3965775075461231 1103 98-99 0.486610836572293 1103 100-101 0.20836460205548235 1104 1 0.2551741113577357 1104 2 0.2866958516324125 1104 3 0.26197715344154204 1104 4 0.16236688223189333 1104 5 0.12643335454386317 1104 6 0.18161450628627307 1104 7 0.1937957132266348 1104 8 0.2366697252102341 1104 9 0.25729178431664224 1104 10-11 0.23850268412633113 1104 12-13 0.2349710059323371 1104 14-15 0.2784440074854402 1104 16-17 0.3428389895878965 1104 18-19 0.2970040615737233 1104 20-21 0.33908129284051114 1104 22-23 0.3143788421798561 1104 24-25 0.30298693344339256 1104 26-27 0.26259557059092486 1104 28-29 0.3310276355052437 1104 30-31 0.5275553998974658 1104 32-33 0.4453284453950772 1104 34-35 0.44572563752380745 1104 36-37 0.49382715518483167 1104 38-39 0.47349058050981085 1104 40-41 0.5410204767092921 1104 42-43 0.4161965980840492 1104 44-45 0.5558325287849399 1104 46-47 0.3929268918097222 1104 48-49 0.4159430953031915 1104 50-51 0.46612954006780427 1104 52-53 0.5330415506020714 1104 54-55 0.5280252839447144 1104 56-57 0.5123342337599013 1104 58-59 0.6361345374002596 1104 60-61 0.4373553307678435 1104 62-63 0.4165043618394364 1104 64-65 0.3029786914150989 1104 66-67 0.4690678297494415 1104 68-69 0.48227678993501755 1104 70-71 0.3650446844337516 1104 72-73 0.4589291400922626 1104 74-75 0.6480964065108097 1104 76-77 0.46533825936927187 1104 78-79 0.49262537866843203 1104 80-81 0.4846317203622412 1104 82-83 0.5025158888452097 1104 84-85 0.4868084360354743 1104 86-87 0.5422846436127138 1104 88-89 0.4796948610523657 1104 90-91 0.47830989137293756 1104 92-93 0.4596325797478187 1104 94-95 0.4297049521100398 1104 96-97 0.7193731495933697 1104 98-99 0.6367961149733574 1104 100-101 0.4202500053026199 1105 1 0.17740966166407546 1105 2 0.341794189825368 1105 3 0.18769583193124362 1105 4 0.17662107596481746 1105 5 0.20924377843845576 1105 6 0.24815506877243365 1105 7 0.17233835643177287 1105 8 0.21691281408600105 1105 9 0.3124668230706078 1105 10-11 0.29210703379784064 1105 12-13 0.249128718433262 1105 14-15 0.39249722268068155 1105 16-17 0.4604761394181125 1105 18-19 0.41161984366569726 1105 20-21 0.2826882722455437 1105 22-23 0.2226167131106891 1105 24-25 0.31291319709955445 1105 26-27 0.3471649747948078 1105 28-29 0.30539909459212566 1105 30-31 0.4209371218654283 1105 32-33 0.44323947966466193 1105 34-35 0.20546828119029925 1105 36-37 0.4559698920453954 1105 38-39 0.3239772542198551 1105 40-41 0.3601980390847217 1105 42-43 0.45772937517492096 1105 44-45 0.44904850833761145 1105 46-47 0.36238795791292233 1105 48-49 0.1768903414646985 1105 50-51 0.32464214894800136 1105 52-53 0.48370059873081317 1105 54-55 0.5081187778917169 1105 56-57 0.5288211635229487 1105 58-59 0.5400989183517595 1105 60-61 0.44557653518579343 1105 62-63 0.4150882272708927 1105 64-65 0.22905567109695113 1105 66-67 0.3463027572241657 1105 68-69 0.3741388027422765 1105 70-71 0.320208246938364 1105 72-73 0.45417699247655463 1105 74-75 0.7383291379273622 1105 76-77 0.3396957047446314 1105 78-79 0.36623525308712246 1105 80-81 0.47027556678580495 1105 82-83 0.5070416833015479 1105 84-85 0.5419493343893542 1105 86-87 0.6575757366591404 1105 88-89 0.6115955470898626 1105 90-91 0.730922900710798 1105 92-93 0.5581509901892474 1105 94-95 0.5804219581814927 1105 96-97 0.7734114052957359 1105 98-99 0.8568352034246729 1105 100-101 0.44851917928948026 1106 1 0.157735529328761 1106 2 0.31155270383080946 1106 3 0.2770551982509417 1106 4 0.10185375373740158 1106 5 0.19972765500639156 1106 6 0.15169955577573546 1106 7 0.17965425180032923 1106 8 0.17438527468656417 1106 9 0.21908596432378857 1106 10-11 0.17376665266863256 1106 12-13 0.18647441464828063 1106 14-15 0.18449400731417143 1106 16-17 0.29210284167097456 1106 18-19 0.2363325713637252 1106 20-21 0.18192276100317883 1106 22-23 0.18316953868596642 1106 24-25 0.23322745600280115 1106 26-27 0.09844040807892185 1106 28-29 0.2711965356391701 1106 30-31 0.13705173656968128 1106 32-33 0.2524392517838905 1106 34-35 0.25329525901989314 1106 36-37 0.281857315841485 1106 38-39 0.3244824249385516 1106 40-41 0.11882694430678953 1106 42-43 0.22440023732076497 1106 44-45 0.42832497320706153 1106 46-47 0.3652846659770077 1106 48-49 0.38563640791743126 1106 50-51 0.33116905057368484 1106 52-53 0.4104904769507485 1106 54-55 0.33570445703852414 1106 56-57 0.4344633139717686 1106 58-59 0.5099196792578695 1106 60-61 0.4916950925744743 1106 62-63 0.5746764885790441 1106 64-65 0.5977842673002769 1106 66-67 0.1898784659022681 1106 68-69 0.43785473027363153 1106 70-71 0.2609611900175146 1106 72-73 0.30298406774482345 1106 74-75 0.3607024329333548 1106 76-77 0.3439054226622389 1106 78-79 0.18703807592125088 1106 80-81 0.24141415739719108 1106 82-83 0.4373194438202468 1106 84-85 0.5265833728677585 1106 86-87 0.5747014694593524 1106 88-89 0.5694215015575708 1106 90-91 0.7304658142951972 1106 92-93 0.4871686221663829 1106 94-95 0.6142661120439534 1106 96-97 0.7185089536130391 1106 98-99 0.9622644352088336 1106 100-101 0.4194063609508376 1107 1 0.09481291058906294 1107 2 0.17975231412860282 1107 3 0.12101285458250288 1107 4 0.13441746888349826 1107 5 0.049828126155951225 1107 6 0.13768039851515113 1107 7 0.05023480328202368 1107 8 0.08732976126925251 1107 9 0.12201099266912507 1107 10-11 0.11694094083283346 1107 12-13 0.20568105423152616 1107 14-15 0.24387371077592235 1107 16-17 0.18590305625127712 1107 18-19 0.26407897873352937 1107 20-21 0.19030375458929427 1107 22-23 0.15439451411403837 1107 24-25 0.07673672637824325 1107 26-27 0.165560969651402 1107 28-29 0.22684002911051238 1107 30-31 0.26391377836110763 1107 32-33 0.25824721875527956 1107 34-35 0.3241552441367759 1107 36-37 0.3493727414604706 1107 38-39 0.2945693319790621 1107 40-41 0.222603183758892 1107 42-43 0.22245542132080232 1107 44-45 0.21020650794670104 1107 46-47 0.2209602054117923 1107 48-49 0.2954755040376398 1107 50-51 0.239441231206321 1107 52-53 0.31078587758239706 1107 54-55 0.21619568739524908 1107 56-57 0.31963485617087883 1107 58-59 0.3013939525747844 1107 60-61 0.21698000716671118 1107 62-63 0.048974494988684114 1107 64-65 0.0074420831450439096 1107 66-67 0.16716362190295797 1107 68-69 0.08609883093871673 1107 70-71 -0.13881433147959754 1107 72-73 0.05547882696453499 1107 74-75 0.016088629341741267 1107 76-77 -0.1101803928896139 1107 78-79 0.07383307872163414 1107 80-81 0.12610422635087915 1107 82-83 0.30245313326462764 1107 84-85 0.40175393196568976 1107 86-87 0.5861791757924699 1107 88-89 0.41299291557282913 1107 90-91 0.09363570873610527 1107 92-93 0.24627406503107352 1107 94-95 0.46892232782996146 1107 96-97 0.5521602832294015 1107 98-99 0.7156790882536122 1107 100-101 0.6525117446950901 1108 1 -0.04815113643402569 1108 2 0.09597385396638103 1108 3 -0.09171906294645993 1108 4 -0.05088922933833118 1108 5 0.013524945941334465 1108 6 -0.018397226197159 1108 7 -0.03057983103814621 1108 8 -0.028034579187341535 1108 9 -0.02773716814914451 1108 10-11 -0.19648671392323536 1108 12-13 -0.18015512680761248 1108 14-15 -0.11733457663162739 1108 16-17 -0.02772648763656349 1108 18-19 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-1.2430242704673837 2316 76-77 -1.4424007350560863 2316 78-79 -1.5341984118212544 2316 80-81 -1.5481998505640568 2316 82-83 -1.6133832061108748 2316 84-85 -1.3272752237107923 2316 86-87 -1.7151408607119798 2316 88-89 -1.8152037392711513 2316 90-91 -2.109333652335202 2316 92-93 -2.135343557572874 2316 94-95 -2.187087155158384 2316 96-97 -2.055534273905689 2316 98-99 -1.650199059016991 2316 100-101 -1.2045297495200487 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 11.0 4 37.0 5 92.0 6 198.0 7 509.0 8 1177.0 9 2165.0 10 3575.0 11 5546.0 12 7869.0 13 10865.0 14 14307.0 15 18937.0 16 23221.0 17 29737.0 18 26505.0 19 19978.0 20 19588.0 21 20544.0 22 22516.0 23 25344.0 24 29770.0 25 35569.0 26 42735.0 27 52843.0 28 64786.0 29 79470.0 30 96488.0 31 117986.0 32 145267.0 33 181781.0 34 234969.0 35 312121.0 36 420778.0 37 486504.0 38 220717.0 39 6883.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.65875605656086 19.526010625387666 15.115162302347112 22.70007101570436 2 15.943731981167286 20.705414153588592 38.96339529642396 24.387458568820165 3 19.63661702117715 24.395551213787456 29.793981345817873 26.173850419217526 4 13.470051790744538 17.133514418176556 36.40211621188724 32.99431757919166 5 13.984719101123597 37.46286741573034 33.66579775280899 14.88661573033708 6 32.30481881361478 36.10921158125973 16.887383646306343 14.698585958819152 7 29.033568624461914 32.78005822295935 20.02493728825686 18.16143586432188 8 26.89348125286733 34.58587468162737 19.124757490296016 19.395886575209285 9 26.500842924770986 16.69357770062203 20.08264222130901 36.72293715329798 10-11 25.35277325366094 26.626866422903657 26.671610238046444 21.34875008538896 12-13 26.434516554672307 23.86013827618565 27.42324880725105 22.282096361890996 14-15 23.00272077414878 25.3447617232828 26.0100795379268 25.642437964641623 16-17 22.236995598608484 28.784305928260718 26.56699910958248 22.411699363548323 18-19 22.15035430801805 26.987991746415485 29.13604140742384 21.725612538142627 20-21 23.035842145067377 25.998835468696885 29.25531656717456 21.710005819061173 22-23 22.61134932202768 26.03220269635742 29.212096720263524 22.14435126135137 24-25 22.683713594074312 26.21323723718541 29.30855211853392 21.794497050206356 26-27 22.40283355031141 26.393216765592133 29.233598201185234 21.970351482911223 28-29 22.02282134366391 26.583764398164583 29.41914781741127 21.974266440760232 30-31 22.24263179182958 26.193339625185384 29.378562761224213 22.18546582176082 32-33 22.06857646426235 26.45323329340581 29.298262550518384 22.179927691813457 34-35 22.821310990331 26.312477012169932 28.837264319687865 22.028947677811203 36-37 22.81839054507069 26.057269023400032 28.917592231725152 22.206748199804125 38-39 22.458926682501296 26.2477680866777 29.153048669553144 22.14025656126786 40-41 22.579104389606915 26.150235062493977 29.107589448146033 22.163071099753072 42-43 22.60126057422084 26.320154068984092 28.647332061409713 22.431253295385357 44-45 22.59759801599465 26.489761320704687 28.467007102041624 22.445633561259044 46-47 22.871513883346097 26.763380899478317 27.977018684902156 22.388086532273434 48-49 23.04944324508272 26.83339202805266 27.689795621931225 22.427369104933394 50-51 22.86730079335346 26.865437674689847 27.703355698906794 22.563905833049898 52-53 23.125034380526728 27.017269540812787 27.43237058270903 22.425325495951455 54-55 22.81020634852438 26.793201879709994 27.631560734187786 22.76503103757784 56-57 22.94624220403188 27.192480632558873 27.384779681311432 22.476497482097816 58-59 23.09163653726123 26.84190259645005 27.367171747678093 22.699289118610626 60-61 22.942912646213152 26.7919594405348 27.9065285681259 22.358599345126144 62-63 22.8370259487676 26.85676863446963 27.781811540259895 22.52439387650287 64-65 22.731557010506748 26.93479595478026 27.66716733047317 22.666479704239823 66-67 22.683273650300574 27.18432709178244 27.378207064170045 22.754192193746945 68-69 22.600647635257634 26.95394807448686 27.650120921516695 22.795283368738808 70-71 22.774533657453734 26.887617208103777 27.555890813948015 22.781958320494482 72-73 22.879071780035655 26.7937188895862 27.618452150567883 22.70875717981026 74-75 22.660721727290017 27.28807714973704 27.211059730208913 22.84014139276403 76-77 22.817466488073013 27.138121793273474 27.2400503278881 22.80436139076542 78-79 22.915176377970592 27.015366206068208 27.263496464925847 22.805960951035352 80-81 22.64222241118116 27.415331165419026 27.173557605620207 22.768888817779604 82-83 22.803558982569484 27.31076703146604 27.118105361143108 22.767568624821376 84-85 22.718283412716747 27.208846838700513 27.02575114625813 23.047118602324606 86-87 22.870818523506728 27.092473000754303 27.049670414694322 22.987038061044647 88-89 22.856049851140853 27.102072558700087 26.895713744604404 23.146163845554657 90-91 22.715992385982094 27.169434191024983 27.126341064567416 22.988232358425506 92-93 22.868350279723295 27.05977737189536 27.123667898695587 22.94820444968576 94-95 22.586032956349662 26.98471907429991 27.084633648956487 23.344614320393934 96-97 22.703726593961935 26.94375144487265 26.9804237957537 23.372098165411717 98-99 22.681624041097727 27.048446080808002 26.700883611155003 23.569046266939267 100-101 22.38963764921775 27.2097637899457 26.746048125039103 23.654550435797447 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 451.0 1 346.0 2 322.5 3 435.0 4 387.5 5 354.0 6 637.0 7 1299.5 8 2082.0 9 2602.0 10 2541.0 11 2307.5 12 2402.5 13 2592.5 14 2834.5 15 3194.5 16 3501.0 17 3749.0 18 4009.5 19 4326.5 20 4701.5 21 5340.5 22 6251.5 23 7348.5 24 8920.0 25 11046.0 26 13906.5 27 17330.5 28 20436.5 29 24274.5 30 29899.0 31 35495.0 32 41205.5 33 46583.0 34 52607.5 35 57459.0 36 62293.0 37 67632.0 38 70390.5 39 72310.0 40 75377.0 41 82658.0 42 89748.5 43 95392.5 44 99867.0 45 102841.5 46 103759.5 47 106770.5 48 117617.5 49 117373.0 50 106988.5 51 100922.0 52 96680.5 53 95412.0 54 93248.5 55 87178.0 56 79773.5 57 71799.0 58 65186.0 59 58129.5 60 49441.5 61 40648.0 62 32908.5 63 26054.0 64 20173.5 65 16021.0 66 12295.5 67 9900.5 68 7545.5 69 5195.0 70 4012.0 71 3298.0 72 2743.5 73 2356.5 74 1943.5 75 1546.5 76 1092.5 77 682.5 78 425.0 79 294.5 80 177.0 81 116.5 82 85.5 83 48.5 84 30.5 85 20.0 86 12.5 87 6.0 88 2.5 89 2.5 90 3.0 91 2.5 92 1.0 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011325272615490815 2 0.009779283020360324 3 0.004925594756578546 4 1.7976623199191771E-4 5 0.005033454495773696 6 0.0 7 4.67392203178986E-4 8 0.0020852882911062453 9 1.0785973919515063E-4 10-11 0.0 12-13 0.0 14-15 4.4941557997979427E-4 16-17 0.003972833727021382 18-19 0.0019055220591143278 20-21 4.853688263781778E-4 22-23 2.8762597118706833E-4 24-25 3.235792175854519E-4 26-27 1.0785973919515063E-4 28-29 8.988311599595886E-5 30-31 5.392986959757531E-4 32-33 0.003613301263037546 34-35 9.707376527563556E-4 36-37 9.34784406357972E-4 38-39 0.00517726748136723 40-41 7.190649279676708E-5 42-43 0.0013841999863377665 44-45 0.005303103843761572 46-47 0.0 48-49 0.0017796856967199853 50-51 0.0011505038847482733 52-53 6.831116815692874E-4 54-55 1.258363623943424E-4 56-57 0.01096574015150698 58-59 0.0019414753055127113 60-61 0.003271745422252902 62-63 1.9774285519110946E-4 64-65 0.002966142827866642 66-67 0.0010785973919515061 68-69 0.0014561064791345335 70-71 0.004278436321407641 72-73 0.0023908908854925054 74-75 0.007226602526075092 76-77 0.0010606207687523144 78-79 0.006813140192493681 80-81 0.002462797378289273 82-83 0.0032178155526553266 84-85 0.0020673116679070535 86-87 5.752519423741367E-4 88-89 0.0024987506246876563 90-91 0.007334462265270242 92-93 0.002516727247886848 94-95 5.033454495773696E-4 96-97 1.0785973919515063E-4 98-99 7.010883047684791E-4 100-101 0.001869568812715944 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2781390.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.209490546925814 #Duplication Level Percentage of deduplicated Percentage of total 1 74.24635141170927 36.5362512793824 2 14.16908115219587 13.945065300352146 3 4.809016313788435 7.099477285001521 4 2.1652946495671612 4.262121863567371 5 1.1771600018006663 2.8963721990414526 6 0.7299755560688829 2.1553035135515164 7 0.4583067700573916 1.578712986811192 8 0.33270766168035265 1.3097899625879286 9 0.24463827185610693 1.0834672253687425 >10 1.4378470272151334 13.412125578222057 >50 0.13925701232453017 4.770131076215541 >100 0.08384492074518796 7.48691291113653 >500 0.005127500779545239 1.6737579815319843 >1k 0.0011720001781687422 0.906015136870445 >5k 2.1975003340663916E-4 0.8844957003591398 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9946 0.35759098867832273 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9164 0.3294755499947868 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5428 0.19515422145042588 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3824 0.13748521422741866 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.595324639838354E-5 0.0 5 0.0 0.0 0.0 3.595324639838354E-5 0.0 6 0.0 0.0 0.0 3.595324639838354E-5 3.595324639838354E-5 7 0.0 0.0 0.0 7.190649279676708E-5 3.595324639838354E-5 8 0.0 0.0 0.0 7.190649279676708E-5 3.595324639838354E-5 9 0.0 0.0 0.0 1.0785973919515063E-4 3.595324639838354E-5 10-11 0.0 0.0 0.0 1.0785973919515063E-4 3.595324639838354E-5 12-13 0.0 0.0 0.0 1.0785973919515063E-4 3.595324639838354E-5 14-15 0.0 0.0 0.0 1.0785973919515063E-4 3.595324639838354E-5 16-17 0.0 0.0 0.0 1.0785973919515063E-4 3.595324639838354E-5 18-19 0.0 0.0 0.0 1.258363623943424E-4 3.595324639838354E-5 20-21 0.0 0.0 0.0 1.7976623199191771E-4 3.595324639838354E-5 22-23 0.0 0.0 0.0 1.7976623199191771E-4 5.392986959757531E-5 24-25 0.0 0.0 0.0 2.516727247886848E-4 1.0785973919515063E-4 26-27 0.0 0.0 0.0 4.134623335814107E-4 1.0785973919515063E-4 28-29 0.0 0.0 0.0 8.808545367603967E-4 1.0785973919515063E-4 30-31 0.0 0.0 0.0 0.0015999194647280677 1.4381298559353417E-4 32-33 0.0 0.0 0.0 0.0030200726974642174 2.1571947839030126E-4 34-35 0.0 0.0 0.0 0.004458202553399559 2.1571947839030126E-4 36-37 0.0 0.0 0.0 0.006148005134123585 2.1571947839030126E-4 38-39 0.0 0.0 0.0 0.008484966150018515 2.1571947839030126E-4 40-41 0.0 0.0 0.0 0.011199436253096474 2.1571947839030126E-4 42-43 0.0 0.0 0.0 0.014291415443357457 2.1571947839030126E-4 44-45 0.0 0.0 0.0 0.017922693329594196 2.1571947839030126E-4 46-47 0.0 0.0 0.0 0.023207820550156577 2.6964934798787653E-4 48-49 0.0 0.0 0.0 0.030200726974642177 3.235792175854519E-4 50-51 0.0 0.0 0.0 0.036079082760777884 3.5953246398383543E-4 52-53 0.0 0.0 0.0 0.04672124369469942 3.95485710382219E-4 54-55 0.0 0.0 0.0 0.06584837077863945 4.314389567806025E-4 56-57 0.0 0.0 0.0 0.10092076264026259 4.314389567806025E-4 58-59 0.0 0.0 0.0 0.13868964798176453 4.314389567806025E-4 60-61 0.0 0.0 0.0 0.1765304398160632 4.314389567806025E-4 62-63 0.0 0.0 0.0 0.22249666533639656 4.673922031789861E-4 64-65 0.0 0.0 0.0 0.2770377401227444 4.673922031789861E-4 66-67 0.0 0.0 0.0 0.35859767957747746 4.673922031789861E-4 68-69 0.0 0.0 0.0 0.46137003440725677 4.673922031789861E-4 70-71 0.0 0.0 0.0 0.5561427919133958 4.673922031789861E-4 72-73 0.0 0.0 0.0 0.6551400558713449 6.112051887725203E-4 74-75 0.0 0.0 0.0 0.761831314558548 6.112051887725203E-4 76-77 0.0 0.0 0.0 0.885097019835406 6.112051887725203E-4 78-79 0.0 0.0 0.0 0.9988710680630908 6.112051887725203E-4 80-81 0.0 0.0 1.797662319919177E-5 1.123934435659868 6.29181811971712E-4 82-83 0.0 0.0 3.595324639838354E-5 1.26823279007978 6.471584351709038E-4 84-85 0.0 0.0 3.595324639838354E-5 1.449131549333247 6.471584351709038E-4 86-87 0.0 0.0 3.595324639838354E-5 1.665947601738699 6.471584351709038E-4 88-89 0.0 0.0 3.595324639838354E-5 1.9220425758343849 6.831116815692873E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12110 0.0 28.679672 1 GTATCAA 16930 0.0 27.025286 1 GTACATG 12900 0.0 25.302765 1 TACATGG 13455 0.0 23.975275 2 ACATGGG 13050 0.0 23.550972 3 ATCAACG 20280 0.0 22.556587 3 GAGTACT 10980 0.0 22.430252 12-13 TCAACGC 20465 0.0 22.305054 4 TATCAAC 20705 0.0 22.165596 2 CAACGCA 20735 0.0 21.945885 5 GTAAGGT 1045 0.0 21.818085 4 AACGCAG 20965 0.0 21.726608 6 TAAGGTG 1180 0.0 21.33465 5 GTACTTT 11705 0.0 20.939676 14-15 AGTACTT 11500 0.0 20.362768 12-13 GTGTAGC 1525 0.0 20.250908 1 ACGCAGA 23340 0.0 19.495432 7 CGCAGAG 23715 0.0 19.207184 8 CATGGGG 6015 0.0 19.031502 4 CATGGGA 7555 0.0 18.861595 4 >>END_MODULE