FastQCFastQC Report
Thu 26 May 2016
SRR936406_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936406_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2795269
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98120.3510216726905353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77480.27718262535734484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46700.16706799953779047No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31910.11415717056211763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA164250.029.0231911
GGTATCA115000.027.2356111
GTACATG130650.025.864841
ATCAACG190250.024.7280643
TCAACGC190200.024.7104644
TACATGG133650.024.4983962
TATCAAC195000.024.3211042
CAACGCA193700.024.1933985
AACGCAG196200.023.9080646
ACATGGG133600.023.2266373
GAGTACT91300.023.13683112-13
ACGCAGA213600.021.892577
GTACTTT97300.021.73179814-15
CGCAGAG217350.021.4496428
AGTACTT94900.020.85699512-13
GCAGAGT225350.020.288219
CATGGGG61500.019.7815364
GTGTAGC17450.019.6104091
AGAGTAC177650.018.5525510-11
ATTCGCG4200.018.09667280-81
Produced by FastQC (version 0.11.5)