##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936406_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2795269 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.264330195054573 31.0 31.0 34.0 28.0 34.0 2 31.32784894763259 31.0 31.0 34.0 28.0 34.0 3 31.365471802534927 31.0 31.0 34.0 28.0 34.0 4 35.00938550100187 37.0 35.0 37.0 32.0 37.0 5 34.92774004934767 37.0 35.0 37.0 32.0 37.0 6 35.04252470871319 37.0 35.0 37.0 32.0 37.0 7 34.874978401005414 37.0 35.0 37.0 32.0 37.0 8 34.85829235039633 37.0 35.0 37.0 32.0 37.0 9 36.35226055166783 39.0 37.0 39.0 32.0 39.0 10-11 36.21831852319043 39.0 35.0 39.0 32.0 39.0 12-13 36.11563591911906 39.0 35.0 39.0 31.0 39.0 14-15 37.18011969509911 39.5 36.5 41.0 31.0 41.0 16-17 37.108328035691734 39.0 36.0 41.0 31.0 41.0 18-19 37.07008788778468 39.0 36.0 41.0 31.0 41.0 20-21 36.96989287971927 39.0 36.0 41.0 31.0 41.0 22-23 36.85862773851103 39.0 36.0 41.0 30.5 41.0 24-25 36.715883158293536 39.0 36.0 41.0 30.0 41.0 26-27 36.516588922211064 39.0 36.0 40.5 30.0 41.0 28-29 36.62280589095361 39.0 36.0 41.0 30.0 41.0 30-31 36.55641854147132 39.0 36.0 41.0 30.0 41.0 32-33 36.41272593084959 39.0 35.0 41.0 30.0 41.0 34-35 36.245598008635305 39.0 35.0 40.0 30.0 41.0 36-37 36.01706740925471 38.5 35.0 40.0 29.0 41.0 38-39 35.83875040291292 38.0 35.0 40.0 28.0 41.0 40-41 35.689642392199104 38.0 35.0 40.0 27.5 41.0 42-43 35.43593389401879 38.0 35.0 40.0 27.0 41.0 44-45 35.17098264961261 38.0 34.0 40.0 26.0 41.0 46-47 34.83965514589114 38.0 34.0 40.0 24.5 41.0 48-49 34.59021546763478 38.0 33.5 40.0 24.0 41.0 50-51 33.8995515279567 37.5 33.0 39.5 23.5 40.5 52-53 34.07883015910097 38.0 33.0 39.5 24.0 40.5 54-55 34.431272446408556 38.0 34.0 40.0 23.5 41.0 56-57 34.360426492047814 38.0 34.0 40.0 23.0 41.0 58-59 34.12460231913279 38.0 33.5 40.0 23.0 41.0 60-61 33.81602861835479 37.5 33.0 40.0 22.0 41.0 62-63 33.4931886698561 37.0 33.0 40.0 22.0 41.0 64-65 33.18422878084363 36.0 32.5 39.5 21.0 41.0 66-67 32.82060277561837 36.0 32.0 39.0 20.0 41.0 68-69 32.4462236013779 35.5 32.0 39.0 19.0 40.5 70-71 32.05182327711572 35.0 31.0 38.5 18.0 40.0 72-73 31.638086352333175 35.0 31.0 37.5 16.0 40.0 74-75 31.20671212681141 35.0 31.0 37.0 13.0 39.0 76-77 30.791056782012753 35.0 30.0 36.5 9.5 39.0 78-79 30.342521775185144 34.0 30.0 36.0 7.0 38.5 80-81 29.94184853049921 34.0 29.5 35.5 6.5 37.0 82-83 29.544064453188582 34.0 29.0 35.0 2.0 37.0 84-85 29.163248689124373 34.0 29.0 35.0 2.0 36.5 86-87 28.815040877997788 34.0 29.0 35.0 2.0 36.0 88-89 28.445134260781344 34.0 28.5 35.0 2.0 36.0 90-91 28.101449270177575 34.0 27.5 35.0 2.0 35.0 92-93 27.745172110448046 34.0 27.0 35.0 2.0 35.0 94-95 27.323636115164586 33.5 26.5 35.0 2.0 35.0 96-97 26.752789981930185 33.0 25.0 35.0 2.0 35.0 98-99 26.0924038080056 33.0 24.5 35.0 2.0 35.0 100-101 23.99916752198089 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29949407914451953 1101 2 0.3272748079156962 1101 3 0.34241262859989874 1101 4 0.29101595066772745 1101 5 0.4131765743269469 1101 6 0.3323667573849747 1101 7 0.31431193809495994 1101 8 0.3523706051486002 1101 9 0.4789941251201739 1101 10-11 0.45684645142357283 1101 12-13 0.5210082548104538 1101 14-15 0.6472298609460196 1101 16-17 0.6370457432884962 1101 18-19 0.6379725319460192 1101 20-21 0.7595786511784297 1101 22-23 0.6663257819542636 1101 24-25 0.7320791513123197 1101 26-27 0.60254968578991 1101 28-29 0.747491375663067 1101 30-31 0.4516174565216886 1101 32-33 0.42797077491002966 1101 34-35 0.4620971274784651 1101 36-37 0.5630454194979251 1101 38-39 0.4714928658281252 1101 40-41 0.2718689108958898 1101 42-43 0.09676777547396398 1101 44-45 0.18366035540910275 1101 46-47 0.16699582797697587 1101 48-49 0.3182425998702243 1101 50-51 0.3595423291328075 1101 52-53 0.3889773949934252 1101 54-55 0.500013503814273 1101 56-57 0.34634127647627366 1101 58-59 0.2705386335266837 1101 60-61 0.3497676987462768 1101 62-63 0.42452295938445417 1101 64-65 0.4073170722826589 1101 66-67 0.45979566658107984 1101 68-69 0.31450357323858924 1101 70-71 0.27258678329349095 1101 72-73 0.25644966358003174 1101 74-75 0.26560378828816766 1101 76-77 0.2377796375184218 1101 78-79 0.22629045156654826 1101 80-81 0.16533153284771984 1101 82-83 0.1441827924263812 1101 84-85 0.13182460197160495 1101 86-87 0.1684758759221019 1101 88-89 0.07524855990969925 1101 90-91 -0.015136829362557336 1101 92-93 -0.16450297834192185 1101 94-95 -0.16332934425545886 1101 96-97 -0.283483249589807 1101 98-99 -0.22788428884852863 1101 100-101 0.11163420645416977 1102 1 0.14728792955030912 1102 2 0.2611288708948436 1102 3 0.1261055189338336 1102 4 0.24236061682045573 1102 5 0.16842772931016015 1102 6 0.14462974157201103 1102 7 0.03539745524400928 1102 8 0.10085065989182596 1102 9 0.32686648291662834 1102 10-11 0.37336779110570006 1102 12-13 0.3768926919313813 1102 14-15 0.40960864353429116 1102 16-17 0.3185560200248787 1102 18-19 0.2919865142636908 1102 20-21 0.1960500056590888 1102 22-23 -0.14159561376440166 1102 24-25 0.13077633954741685 1102 26-27 0.2498349034646239 1102 28-29 0.3245158849999896 1102 30-31 0.17863038980328128 1102 32-33 0.1338612731043085 1102 34-35 0.020339013939690176 1102 36-37 -0.04745665256731968 1102 38-39 0.09084978216162654 1102 40-41 -0.12860183066480602 1102 42-43 -0.002303144707262561 1102 44-45 0.012860768448817339 1102 46-47 0.09125830997623297 1102 48-49 0.20482288605885657 1102 50-51 0.020203324595705396 1102 52-53 0.03083570818535719 1102 54-55 -0.11120005499603991 1102 56-57 0.1236978850521595 1102 58-59 0.04472888548242082 1102 60-61 -0.38324196642186337 1102 62-63 -0.29390244404999066 1102 64-65 0.03775439845934869 1102 66-67 -0.06304214417870213 1102 68-69 0.03364203140318267 1102 70-71 0.2028233965668491 1102 72-73 -0.01816453909901483 1102 74-75 -0.1063532528136939 1102 76-77 -0.08961597063107618 1102 78-79 -0.09350226729469924 1102 80-81 -0.004189389317826198 1102 82-83 -0.02348493358542214 1102 84-85 -0.10614076112994297 1102 86-87 -0.13671846933977605 1102 88-89 -0.4626541811120468 1102 90-91 -0.6493050408549799 1102 92-93 -0.5765893533410136 1102 94-95 -0.4366664501248394 1102 96-97 -0.5535628934993149 1102 98-99 -0.5154410188188976 1102 100-101 0.023173030251253834 1103 1 0.20120778431269315 1103 2 0.394977366810469 1103 3 0.32689650193972497 1103 4 0.2764151878717769 1103 5 0.3381277567392118 1103 6 0.18828881246086127 1103 7 0.3508092082595269 1103 8 0.1344215962618236 1103 9 0.5289960125808193 1103 10-11 0.47844511095556896 1103 12-13 0.4191404761203401 1103 14-15 0.47135649580879857 1103 16-17 0.6759109583051739 1103 18-19 0.5323791459572647 1103 20-21 0.3731135551263236 1103 22-23 0.23884660239085775 1103 24-25 0.396726271451044 1103 26-27 0.4525925736963501 1103 28-29 0.5098775361469734 1103 30-31 0.140291841629562 1103 32-33 0.2699823540992625 1103 34-35 0.37188676398845644 1103 36-37 0.4254612665738975 1103 38-39 0.40519673787181887 1103 40-41 0.34815101491046363 1103 42-43 0.5569653762387361 1103 44-45 0.6456543713951319 1103 46-47 0.6907783231213926 1103 48-49 0.8406017533015699 1103 50-51 0.29204406330546107 1103 52-53 0.47188001271587154 1103 54-55 0.6755767471655503 1103 56-57 0.6484203525675554 1103 58-59 0.5201006444643568 1103 60-61 0.696920644374103 1103 62-63 0.6037138366434363 1103 64-65 0.584191446174863 1103 66-67 0.5173891826191479 1103 68-69 0.382000693497325 1103 70-71 0.46539443446981466 1103 72-73 0.4367783632156552 1103 74-75 0.3612368371349959 1103 76-77 0.40617598548935874 1103 78-79 0.40760766780429947 1103 80-81 0.5654698084331251 1103 82-83 0.5159768095907076 1103 84-85 0.3133764961201919 1103 86-87 0.29007858312982293 1103 88-89 0.29520547780210293 1103 90-91 0.47718453339712497 1103 92-93 0.4529003471237232 1103 94-95 0.33218859131208944 1103 96-97 0.5783414840873142 1103 98-99 0.3899449699336728 1103 100-101 0.3526318919691853 1104 1 0.3447611993827415 1104 2 0.3208717257511644 1104 3 0.3236053931821914 1104 4 0.3737938634865827 1104 5 0.28999041453801766 1104 6 0.32477696631674746 1104 7 0.39298245500651063 1104 8 0.4392021748840591 1104 9 0.6232812524389146 1104 10-11 0.5808417901360912 1104 12-13 0.5363017744635599 1104 14-15 0.46201815809202884 1104 16-17 0.28685680459664553 1104 18-19 0.5526724148818687 1104 20-21 0.5256995070201711 1104 22-23 0.6433754331990684 1104 24-25 0.5642433268601224 1104 26-27 0.555716698213601 1104 28-29 0.5327850006228871 1104 30-31 0.5814383189532393 1104 32-33 0.42971094113612907 1104 34-35 0.4852494588441516 1104 36-37 0.3793290719123945 1104 38-39 0.4520743629616675 1104 40-41 0.3524941700236752 1104 42-43 0.497382126129466 1104 44-45 0.3280287968774047 1104 46-47 0.6090006549325366 1104 48-49 0.4946203725985825 1104 50-51 0.216168113274243 1104 52-53 0.4149710533582649 1104 54-55 0.7670251784200133 1104 56-57 0.7385263678906355 1104 58-59 0.6922096034742111 1104 60-61 0.6358777763920642 1104 62-63 0.5422716018235576 1104 64-65 0.4958118366393549 1104 66-67 0.49297162247558646 1104 68-69 0.6418678798443906 1104 70-71 0.450408614981022 1104 72-73 0.3029151090290405 1104 74-75 0.07338332684719973 1104 76-77 0.227003775683432 1104 78-79 0.3316296211352139 1104 80-81 0.5498189752397984 1104 82-83 0.5505639881656066 1104 84-85 0.5491201867218471 1104 86-87 0.4435895990256036 1104 88-89 0.4700665361774732 1104 90-91 0.5479812979497787 1104 92-93 0.4351189054344289 1104 94-95 0.4255397474546321 1104 96-97 0.7447881820353608 1104 98-99 0.7445901115790363 1104 100-101 0.8490442737104154 1105 1 0.17270208228642403 1105 2 0.21721288174510534 1105 3 0.2562792493049919 1105 4 0.2719963632879754 1105 5 0.459589526338938 1105 6 0.34080043290108364 1105 7 0.40767336497304285 1105 8 0.08043108534273102 1105 9 0.5070892167974321 1105 10-11 0.45061061048271256 1105 12-13 0.3840415935364234 1105 14-15 0.45956331274481244 1105 16-17 0.44104659114571376 1105 18-19 0.34090024035393185 1105 20-21 0.0859708729949773 1105 22-23 0.021677195353618117 1105 24-25 -0.14622155329632136 1105 26-27 -0.0016666555140716355 1105 28-29 0.028365773752973666 1105 30-31 0.10304377448044733 1105 32-33 0.06779738940296909 1105 34-35 0.031487646997149454 1105 36-37 -0.04097665716732024 1105 38-39 -0.08812765330958428 1105 40-41 -0.1930858022394517 1105 42-43 -0.1252261438269926 1105 44-45 0.07939787625342376 1105 46-47 -0.0072958696596145955 1105 48-49 0.19088464536462624 1105 50-51 0.11616133009236052 1105 52-53 0.1883781101678963 1105 54-55 0.10313748426218439 1105 56-57 0.3143331553509725 1105 58-59 0.20736555377058608 1105 60-61 0.2700527151902463 1105 62-63 0.053710293200495585 1105 64-65 0.07167844724892092 1105 66-67 -0.11924316810738134 1105 68-69 0.05838626070855213 1105 70-71 0.19973306489023201 1105 72-73 0.15339426934207268 1105 74-75 0.09925659701488243 1105 76-77 0.028340772006643533 1105 78-79 0.17916427851841377 1105 80-81 0.20760540691955498 1105 82-83 0.0876326259038116 1105 84-85 -0.18029057192060804 1105 86-87 0.024082963170993565 1105 88-89 0.10274240253008671 1105 90-91 0.10397254065772543 1105 92-93 -0.0378683358411962 1105 94-95 0.1271666576163888 1105 96-97 0.05900892397356117 1105 98-99 -0.1270387088385334 1105 100-101 0.0373975396727424 1106 1 0.05932131219785575 1106 2 0.056095694221237835 1106 3 -0.009212802645283347 1106 4 0.1271304398446631 1106 5 0.2369165802598019 1106 6 0.167554182370246 1106 7 0.3559835451369864 1106 8 0.29507731082109245 1106 9 0.3259109562333862 1106 10-11 0.4144342288541196 1106 12-13 0.4364719125277219 1106 14-15 0.35062744707494886 1106 16-17 0.1870015333569981 1106 18-19 0.22733119482259667 1106 20-21 0.3180308583912179 1106 22-23 0.2841255246286636 1106 24-25 0.09370933854115293 1106 26-27 0.1890074851402872 1106 28-29 -0.018100834661602505 1106 30-31 -0.04964570012031544 1106 32-33 -0.07630244775595685 1106 34-35 -0.013284100535031484 1106 36-37 0.09988583530929418 1106 38-39 0.025153441544574662 1106 40-41 -0.0458460042479274 1106 42-43 0.15121949077963848 1106 44-45 -0.05434604775814478 1106 46-47 0.010161426567542264 1106 48-49 0.12439973642425173 1106 50-51 0.20625552127938818 1106 52-53 0.1368066785412907 1106 54-55 0.30134798919171146 1106 56-57 0.33249085150114155 1106 58-59 0.278522978009768 1106 60-61 0.29664117944311386 1106 62-63 0.3152264108004772 1106 64-65 0.24241066686744972 1106 66-67 0.1438937222697234 1106 68-69 0.173718908663254 1106 70-71 0.31173747656956863 1106 72-73 0.41680383449835645 1106 74-75 0.2815389231064209 1106 76-77 0.13572384264545434 1106 78-79 0.290984997524383 1106 80-81 0.3393978349624369 1106 82-83 0.2762040391993388 1106 84-85 0.28006770899093425 1106 86-87 0.39116202816382994 1106 88-89 0.5091557591642548 1106 90-91 0.5147059212445022 1106 92-93 0.36900406152768284 1106 94-95 0.4177153831482201 1106 96-97 0.5004613716306956 1106 98-99 0.546670019504063 1106 100-101 0.8599149007308142 1107 1 0.43087700878184165 1107 2 0.37540434890980023 1107 3 0.5038494567499043 1107 4 0.37706308546483314 1107 5 0.42146059609756037 1107 6 0.374981225103852 1107 7 0.5515542213350457 1107 8 0.5968478828443793 1107 9 0.7552666891388427 1107 10-11 0.7545187161924929 1107 12-13 0.7523958848994567 1107 14-15 0.790346524429701 1107 16-17 0.7522634371210586 1107 18-19 0.8337209302146746 1107 20-21 0.9210119632425275 1107 22-23 0.8325745278836223 1107 24-25 0.878869322655504 1107 26-27 0.9074963621881125 1107 28-29 0.7683713125561056 1107 30-31 0.7797598750262225 1107 32-33 0.9911552087905875 1107 34-35 0.8507710622031297 1107 36-37 0.9404911965183658 1107 38-39 0.9458868159890272 1107 40-41 0.8594829246078888 1107 42-43 1.058956712905406 1107 44-45 1.1081551247069825 1107 46-47 1.3265546344520942 1107 48-49 1.2585833813838505 1107 50-51 1.1174941733136947 1107 52-53 1.3340138167085698 1107 54-55 1.4245643454295305 1107 56-57 1.3007786632521388 1107 58-59 1.3489080554722932 1107 60-61 1.2741123866930337 1107 62-63 1.210927155789804 1107 64-65 1.0661754374334862 1107 66-67 1.0145573475119463 1107 68-69 1.2296055816762035 1107 70-71 1.2231213147115128 1107 72-73 1.2143033904583724 1107 74-75 1.10455730735967 1107 76-77 1.2106588331958825 1107 78-79 1.2313429436448686 1107 80-81 1.4340040463334525 1107 82-83 1.389161575006927 1107 84-85 1.4408977444542614 1107 86-87 1.5524241295007393 1107 88-89 1.6096457498044892 1107 90-91 1.444921545065803 1107 92-93 1.4356028250010517 1107 94-95 1.3912119336061295 1107 96-97 1.586499357505236 1107 98-99 1.8235758491004752 1107 100-101 2.103030035808775 1108 1 0.1732873007240947 1108 2 0.026092676024838113 1108 3 0.05926425883131614 1108 4 0.1410286804761398 1108 5 0.2593235734300947 1108 6 0.2297902920904491 1108 7 0.38758542287972375 1108 8 0.1024727688508591 1108 9 0.3944886242567023 1108 10-11 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-2.2286763072202334 2316 64-65 -2.2025929431420934 2316 66-67 -2.9383416111948506 2316 68-69 -2.219915284724898 2316 70-71 -1.9630614920297802 2316 72-73 -1.9360227681071756 2316 74-75 -2.104862645665335 2316 76-77 -2.814400831421107 2316 78-79 -2.7723590701785845 2316 80-81 -2.8554334547550404 2316 82-83 -2.6393586824367397 2316 84-85 -2.736923608211967 2316 86-87 -2.8294901498305585 2316 88-89 -3.0030423048407293 2316 90-91 -3.0490460829008406 2316 92-93 -3.3373662508910193 2316 94-95 -3.3713610256808053 2316 96-97 -3.572476443943412 2316 98-99 -4.046389442127115 2316 100-101 -4.621945807077804 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8099.0 3 3242.0 4 3230.0 5 3445.0 6 3839.0 7 4337.0 8 5355.0 9 6463.0 10 7971.0 11 9606.0 12 11659.0 13 13861.0 14 15503.0 15 18259.0 16 22087.0 17 26109.0 18 23796.0 19 17689.0 20 18096.0 21 19509.0 22 21361.0 23 24272.0 24 27610.0 25 32121.0 26 38317.0 27 45050.0 28 53653.0 29 64246.0 30 78295.0 31 96741.0 32 120648.0 33 154568.0 34 202586.0 35 274728.0 36 379970.0 37 492902.0 38 386886.0 39 59138.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.15340975364361 18.46402761607417 14.399615870941796 22.982946759340425 2 15.581228196272987 19.73634640820548 40.24021262871153 24.44221276681 3 19.433202291491057 24.289347257065742 29.37529248281596 26.90215796862724 4 12.885618786547093 16.32335363627905 37.03268034670083 33.75834723047303 5 14.08350542398723 37.13797435282581 33.69600618452652 15.082514038660449 6 32.15948232816308 35.572650129450174 17.47801065167505 14.789856890711695 7 29.232753302482962 32.405305442995754 20.301807366014877 18.0601338885064 8 26.731655056996345 34.203995988052895 19.52882880271719 19.53552015223357 9 26.670857660538083 16.875166822785364 20.221068367108163 36.23290714956839 10-11 25.28267791807038 26.353387750748812 26.941327040984138 21.42260729019667 12-13 26.418134078326254 23.69788632952659 27.453934073351526 22.430045518795627 14-15 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28.85815805446222 22.43796137767842 40-41 22.59834392602941 26.2151965984119 28.828852679537704 22.35760679602099 42-43 22.610756759599422 26.280146262566422 28.438434957626285 22.670662020207875 44-45 22.57332624268374 26.542229701629257 28.230122703655386 22.654321352031612 46-47 22.751061153077046 26.673029977742125 27.880031790319837 22.695877078860992 48-49 22.935295706474008 26.738294001799478 27.636154840971578 22.69025545075494 50-51 22.71781664256598 26.87403987810611 27.632120285430332 22.776023193897576 52-53 22.97783306215844 26.906702956879613 27.43687134289529 22.678592638066657 54-55 22.671401916101726 26.700710663058345 27.62012395148623 23.007763469353694 56-57 22.840324262384286 27.080209308403262 27.41912893345409 22.660337495758363 58-59 22.956054944288322 26.735272494405578 27.404528127733485 22.90414443357261 60-61 22.860035524614123 26.635339657167794 27.948963328795866 22.555661489422217 62-63 22.77259424580549 26.80020484893822 27.75973215804564 22.667468747210652 64-65 22.57825336048734 26.874972608976027 27.652260553258433 22.894513477278196 66-67 22.54520879923669 27.1008447705117 27.385261217724548 22.96868521252706 68-69 22.485492365155917 26.87812155644919 27.642625613578144 22.99376046481675 70-71 22.600201431487356 26.79813380703722 27.574950939089337 23.026713822386085 72-73 22.695241618344905 26.72818866380075 27.665007126624957 22.911562591229387 74-75 22.481569602799965 27.193451151688524 27.329095440595296 22.995883804916215 76-77 22.65387468501677 27.042594801745928 27.309821859956852 22.99370865328045 78-79 22.771201678824294 27.00086406916275 27.314874210747348 22.91306004126561 80-81 22.549649839434075 27.304401469023006 27.298640454168353 22.847308237374566 82-83 22.639528585463026 27.232428746527038 27.284965951884004 22.843076716125935 84-85 22.618728175625392 27.114764010532944 27.1666952887973 23.099812525044364 86-87 22.773772611460068 26.987279927286274 27.192713028168697 23.046234433084962 88-89 22.755582634510844 26.95964410811205 27.057028172508605 23.227745084868495 90-91 22.643144459779656 27.05011453703513 27.291478641369828 23.015262361815385 92-93 22.762182103897135 26.86227659747275 27.3612570615253 23.014284237104814 94-95 22.544724952810004 26.82624207358348 27.327944348005285 23.301088625601235 96-97 22.585709208066003 26.87141557193345 27.25235929848111 23.29051592151944 98-99 22.544117378688615 26.89158952812287 26.978559367247456 23.585733725941058 100-101 22.389290155341207 26.835059595270742 27.282120309311686 23.493529940076364 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 213.0 1 187.0 2 262.0 3 377.5 4 351.5 5 381.0 6 635.0 7 1292.0 8 2224.5 9 2681.5 10 2494.0 11 2282.5 12 2312.0 13 2500.5 14 2739.5 15 2948.0 16 3180.5 17 3362.0 18 3495.5 19 3742.0 20 4180.5 21 4861.0 22 5734.0 23 6870.0 24 8476.5 25 10623.5 26 13489.5 27 16944.5 28 20203.5 29 23915.5 30 29419.5 31 35176.5 32 41322.5 33 46456.5 34 52530.0 35 57851.0 36 62590.0 37 68020.0 38 70681.0 39 72698.0 40 75628.0 41 82878.5 42 90174.5 43 95739.5 44 99733.0 45 103394.0 46 105171.0 47 107750.0 48 118529.5 49 118178.0 50 107320.5 51 101435.0 52 97984.0 53 96565.0 54 94056.5 55 88220.5 56 80596.5 57 72549.5 58 66322.5 59 59551.5 60 50575.0 61 41352.5 62 33799.5 63 26759.0 64 20468.5 65 16467.5 66 12728.0 67 10101.0 68 7635.0 69 5207.5 70 3985.5 71 3228.5 72 2700.5 73 2371.5 74 1910.5 75 1534.5 76 1113.5 77 652.0 78 408.0 79 275.5 80 188.0 81 132.0 82 96.0 83 59.0 84 36.0 85 27.5 86 22.0 87 16.5 88 11.5 89 6.5 90 6.0 91 7.5 92 4.0 93 1.5 94 0.5 95 2.0 96 2.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01341552458815234 2 0.00579550662208181 3 0.008335512610771987 4 0.033234726246382726 5 0.04289390394985241 6 0.05473534031966154 7 0.0252211862257264 8 0.022037235056804907 9 0.013844821374973214 10-11 0.04250038189526661 12-13 0.04108727997198123 14-15 0.018978495450706175 16-17 0.02173314983280679 18-19 0.02928161833440717 20-21 0.023289350685032458 22-23 0.013665947713797848 24-25 0.003398599562331926 26-27 0.002450569158102494 28-29 0.0016277503166958172 30-31 0.00670776229407617 32-33 0.00166352504893089 34-35 0.001860286076223791 36-37 0.005956492917139638 38-39 0.0062784655072552955 40-41 0.003720572152447582 42-43 7.870441091716038E-4 44-45 0.0013773271910503067 46-47 0.002808316480453223 48-49 5.008462512910206E-4 50-51 0.0018245113439887182 52-53 8.943683058768226E-4 54-55 0.0022359207646920564 56-57 0.0022716954969271294 58-59 0.0026473301853953946 60-61 8.585935736417496E-4 62-63 0.0013773271910503067 64-65 0.0036311353218598996 66-67 0.0025757807209252494 68-69 0.006761424392428778 70-71 0.009802276632409975 72-73 0.00418564367150353 74-75 0.003237613267274098 76-77 0.002879865944923369 78-79 0.03335993780920548 80-81 0.02226977081633288 82-83 0.0038278963491528003 84-85 0.008192413681831696 86-87 0.007101284348661972 88-89 0.005384097201378472 90-91 0.011501576413575939 92-93 0.013236650926976975 94-95 0.006618325463488488 96-97 0.0037742342508001914 98-99 0.022251883450215348 100-101 0.04040756005951484 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2795269.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.84793321161313 #Duplication Level Percentage of deduplicated Percentage of total 1 76.23726775413328 39.5274476675217 2 13.242843449411504 13.732281253938721 3 4.425839250702891 6.884118536273381 4 1.9639856868184518 4.07314394874909 5 1.0313663262247434 2.6737106199403646 6 0.6326563253812373 1.968115374256258 7 0.4370216744139065 1.5861069410927697 8 0.30274007788113966 1.2557157870767908 9 0.21459394708582433 1.0013627372510037 >10 1.305590263618224 12.782033974900997 >50 0.12703592342652045 4.580926937249127 >100 0.0742122440587946 7.09533057049565 >500 0.003739173565033829 1.3132072431596264 >1k 9.694153686982865E-4 0.8960670233925627 >5k 1.384879098140409E-4 0.6304313847018471 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9812 0.3510216726905353 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7748 0.27718262535734484 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4670 0.16706799953779047 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3191 0.11415717056211763 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 3.57747322350729E-5 10-11 0.0 0.0 0.0 1.788736611753645E-5 3.57747322350729E-5 12-13 0.0 0.0 0.0 3.57747322350729E-5 3.57747322350729E-5 14-15 0.0 0.0 0.0 3.57747322350729E-5 3.57747322350729E-5 16-17 0.0 0.0 0.0 7.15494644701458E-5 5.366209835260936E-5 18-19 3.57747322350729E-5 0.0 0.0 1.430989289402916E-4 7.15494644701458E-5 20-21 3.57747322350729E-5 0.0 0.0 1.788736611753645E-4 7.15494644701458E-5 22-23 3.57747322350729E-5 0.0 0.0 2.1464839341043743E-4 7.15494644701458E-5 24-25 3.57747322350729E-5 0.0 0.0 2.861978578805832E-4 7.15494644701458E-5 26-27 3.57747322350729E-5 0.0 0.0 4.471841529384113E-4 7.15494644701458E-5 28-29 3.57747322350729E-5 0.0 0.0 7.333820108189946E-4 7.15494644701458E-5 30-31 3.57747322350729E-5 0.0 0.0 0.0014488766555204526 8.943683058768227E-5 32-33 3.57747322350729E-5 0.0 0.0 0.002665217551512931 1.0732419670521871E-4 34-35 3.57747322350729E-5 0.0 0.0 0.0040604321086807746 1.0732419670521871E-4 36-37 3.57747322350729E-5 0.0 0.0 0.005902830818787029 1.0732419670521871E-4 38-39 3.57747322350729E-5 0.0 0.0 0.007727342162775747 1.0732419670521871E-4 40-41 3.57747322350729E-5 0.0 0.0 0.0093729798455891 1.0732419670521871E-4 42-43 3.57747322350729E-5 0.0 0.0 0.01194876056651435 1.2521156282275516E-4 44-45 3.57747322350729E-5 0.0 0.0 0.014631865484144817 1.430989289402916E-4 46-47 3.57747322350729E-5 0.0 0.0 0.01935413013917444 1.6098629505782806E-4 48-49 3.57747322350729E-5 0.0 0.0 0.025775694575370028 2.1464839341043743E-4 50-51 3.57747322350729E-5 0.0 0.0 0.031535426465216766 2.1464839341043743E-4 52-53 3.57747322350729E-5 0.0 0.0 0.04130192836539166 2.1464839341043743E-4 54-55 3.57747322350729E-5 0.0 0.0 0.05890309662504753 2.3253575952797388E-4 56-57 3.57747322350729E-5 0.0 0.0 0.0889180969702737 2.504231256455103E-4 58-59 3.57747322350729E-5 0.0 0.0 0.12442451871358357 2.504231256455103E-4 60-61 3.57747322350729E-5 0.0 0.0 0.16132615501406125 2.504231256455103E-4 62-63 3.57747322350729E-5 0.0 0.0 0.2041842842316786 3.219725901156561E-4 64-65 3.57747322350729E-5 0.0 0.0 0.25675525325111825 3.3985995623319254E-4 66-67 3.57747322350729E-5 0.0 0.0 0.33519135367651554 3.57747322350729E-4 68-69 3.57747322350729E-5 0.0 0.0 0.42949354784816773 3.57747322350729E-4 70-71 3.57747322350729E-5 0.0 0.0 0.5186441805779695 3.57747322350729E-4 72-73 3.57747322350729E-5 0.0 0.0 0.6093867888922317 4.2929678682087486E-4 74-75 3.57747322350729E-5 0.0 0.0 0.7101820969645498 4.2929678682087486E-4 76-77 3.57747322350729E-5 0.0 0.0 0.8283102628047605 4.2929678682087486E-4 78-79 3.57747322350729E-5 0.0 0.0 0.9372443224605576 4.2929678682087486E-4 80-81 3.57747322350729E-5 0.0 0.0 1.0578409448249881 4.829588851734842E-4 82-83 3.57747322350729E-5 0.0 0.0 1.1939638009794407 5.366209835260936E-4 84-85 3.57747322350729E-5 0.0 0.0 1.3672029418277811 5.366209835260936E-4 86-87 3.57747322350729E-5 0.0 0.0 1.5847312011831418 5.366209835260936E-4 88-89 3.57747322350729E-5 0.0 0.0 1.8405563113961483 5.366209835260936E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16425 0.0 29.023191 1 GGTATCA 11500 0.0 27.235611 1 GTACATG 13065 0.0 25.86484 1 ATCAACG 19025 0.0 24.728064 3 TCAACGC 19020 0.0 24.710464 4 TACATGG 13365 0.0 24.498396 2 TATCAAC 19500 0.0 24.321104 2 CAACGCA 19370 0.0 24.193398 5 AACGCAG 19620 0.0 23.908064 6 ACATGGG 13360 0.0 23.226637 3 GAGTACT 9130 0.0 23.136831 12-13 ACGCAGA 21360 0.0 21.89257 7 GTACTTT 9730 0.0 21.731798 14-15 CGCAGAG 21735 0.0 21.449642 8 AGTACTT 9490 0.0 20.856995 12-13 GCAGAGT 22535 0.0 20.28821 9 CATGGGG 6150 0.0 19.781536 4 GTGTAGC 1745 0.0 19.610409 1 AGAGTAC 17765 0.0 18.55255 10-11 ATTCGCG 420 0.0 18.096672 80-81 >>END_MODULE