FastQCFastQC Report
Thu 26 May 2016
SRR936406_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936406_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2795269
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103060.36869439041466134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90030.3220799143123614No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55940.20012385212299783No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38120.1363732792800979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120400.027.179081
GTATCAA167200.027.1189211
GTACATG127800.024.0284611
ATCAACG201350.022.4613193
TACATGG132050.022.3786072
TCAACGC203900.022.2021264
TATCAAC206100.022.0604822
CAACGCA206500.021.8940945
TACACCG2657.9671736E-1021.5052995
AACGCAG211900.021.3585626
ACATGGG131300.021.3108393
GAGTACT111450.021.17655812-13
AGTACTT116400.020.398412-13
GTACTTT118550.019.94834314-15
GTACCGT2201.3486406E-619.4280826
ACGCAGA236100.019.149227
CGCAGAG238500.018.8967868
CATGGGG59600.018.7301164
AGAGTAC197550.018.71374110-11
CGTATAG1600.001113584817.9931891
Produced by FastQC (version 0.11.5)