FastQCFastQC Report
Thu 26 May 2016
SRR936405_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936405_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2817673
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96580.342765111494485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76360.2710037680028875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46190.16392959722437628No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32750.11623066267803255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA159850.027.7648371
GGTATCA114500.027.348961
GTACATG133050.024.2145121
ATCAACG189050.023.2752933
TCAACGC190650.023.1547364
TACATGG134950.023.0285052
TATCAAC191600.022.9407232
CAACGCA193750.022.7838555
AACGCAG196700.022.4168036
ACATGGG134900.022.2973183
GAGTACT91800.022.15450312-13
AGTACTT97100.020.33352712-13
GTACTTT99400.020.19766814-15
ACGCAGA218350.020.1077867
CGCAGAG221000.019.8441168
CATGGGG61200.018.6346264
GCAGAGT230050.018.6294049
GTATAGG7700.018.5136241
AGAGTAC182700.018.15374410-11
TATGACG3951.8189894E-1118.0449244
Produced by FastQC (version 0.11.5)