##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936405_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2817673 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.267425993009123 31.0 31.0 34.0 28.0 34.0 2 31.316751447027386 31.0 31.0 34.0 28.0 34.0 3 31.369957408116555 31.0 31.0 34.0 28.0 34.0 4 34.99416717269889 37.0 35.0 37.0 32.0 37.0 5 34.96069345165319 37.0 35.0 37.0 32.0 37.0 6 35.08422269014183 37.0 35.0 37.0 32.0 37.0 7 34.94149037166485 37.0 35.0 37.0 32.0 37.0 8 34.88336545795059 37.0 35.0 37.0 32.0 37.0 9 36.34733732409687 39.0 37.0 39.0 32.0 39.0 10-11 36.254414547039346 39.0 35.0 39.0 32.0 39.0 12-13 36.13652968247203 39.0 35.0 39.0 31.0 39.0 14-15 37.18105898022943 39.5 36.5 41.0 31.0 41.0 16-17 37.050159475567256 39.0 36.0 41.0 31.0 41.0 18-19 36.992611811235726 39.0 36.0 41.0 31.0 41.0 20-21 36.89762154799368 39.0 36.0 41.0 30.5 41.0 22-23 36.75890708396609 39.0 36.0 41.0 30.0 41.0 24-25 36.90296407709482 39.0 36.0 41.0 30.0 41.0 26-27 36.883440519889994 39.0 36.0 41.0 30.0 41.0 28-29 36.785793809288734 39.0 36.0 41.0 30.0 41.0 30-31 36.598992147066035 39.0 36.0 41.0 30.0 41.0 32-33 36.424502772323116 39.0 35.0 41.0 30.0 41.0 34-35 36.210197208831545 39.0 35.0 40.0 30.0 41.0 36-37 36.02697119218589 38.5 35.0 40.0 29.0 41.0 38-39 35.82798660454921 38.0 35.0 40.0 28.5 41.0 40-41 35.64027355197001 38.0 35.0 40.0 27.0 41.0 42-43 35.40190398247064 38.0 34.5 40.0 27.0 41.0 44-45 35.14515027116347 38.0 34.0 40.0 26.0 41.0 46-47 34.87056766345846 38.0 34.0 40.0 24.5 41.0 48-49 34.76914265778889 38.0 34.0 40.0 24.0 41.0 50-51 33.85750067520255 37.5 33.0 39.5 23.5 40.5 52-53 33.99722678962392 37.5 33.0 39.5 23.5 40.5 54-55 34.41636680338705 38.0 34.0 40.0 24.0 41.0 56-57 34.33215050149538 38.0 34.0 40.0 23.0 41.0 58-59 34.0719682518163 38.0 33.5 40.0 23.0 41.0 60-61 33.75941832143049 37.0 33.0 40.0 22.0 41.0 62-63 33.42087602074478 37.0 33.0 40.0 21.5 41.0 64-65 33.08080036256868 36.0 32.0 39.5 20.5 41.0 66-67 32.6960282474226 36.0 32.0 39.0 19.5 41.0 68-69 32.294093388409514 35.0 31.5 39.0 18.0 40.0 70-71 31.86085344182948 35.0 31.0 38.0 16.5 40.0 72-73 31.46233310252822 35.0 31.0 37.5 13.5 39.5 74-75 31.033445683725542 35.0 30.5 37.0 11.0 39.0 76-77 30.601029643965074 34.5 30.0 36.0 7.5 39.0 78-79 30.161389912882015 34.0 30.0 36.0 7.0 38.5 80-81 29.694854229003862 34.0 29.0 35.5 2.0 37.0 82-83 29.21610864709993 34.0 29.0 35.0 2.0 37.0 84-85 28.901464257917794 34.0 29.0 35.0 2.0 36.0 86-87 28.566621286430326 34.0 29.0 35.0 2.0 36.0 88-89 28.16391682072405 34.0 27.5 35.0 2.0 36.0 90-91 27.76508860325524 33.5 27.0 35.0 2.0 35.0 92-93 27.392976225417215 33.0 26.0 35.0 2.0 35.0 94-95 26.92188199269397 33.0 25.0 35.0 2.0 35.0 96-97 26.33425383286137 33.0 25.0 35.0 2.0 35.0 98-99 25.611630057852704 33.0 22.0 35.0 2.0 35.0 100-101 23.119350435625424 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3311076835289022 1101 2 0.4209982242143191 1101 3 0.45618095605226827 1101 4 0.4578383094714127 1101 5 0.30230213704498965 1101 6 0.22321338797993917 1101 7 0.33792796715047757 1101 8 0.447319271282268 1101 9 0.5755391005647681 1101 10-11 0.507648539608553 1101 12-13 0.6172824365827481 1101 14-15 0.7539363093225546 1101 16-17 0.7923286925038582 1101 18-19 0.8061162093235694 1101 20-21 0.8002292287889006 1101 22-23 0.7524324181351076 1101 24-25 0.7023053928172374 1101 26-27 0.586950222506978 1101 28-29 0.7535266421378779 1101 30-31 0.7436154460516562 1101 32-33 0.6073071731181514 1101 34-35 0.7406058904167594 1101 36-37 0.6467501033123497 1101 38-39 0.6871098990958515 1101 40-41 0.7155078555971031 1101 42-43 0.6640267875264811 1101 44-45 0.580915644797777 1101 46-47 0.7818512992134217 1101 48-49 0.9167150328675149 1101 50-51 0.9354122871109141 1101 52-53 0.895035884632776 1101 54-55 1.002334601051139 1101 56-57 0.8803655411612681 1101 58-59 0.9093409383653679 1101 60-61 0.906612499862824 1101 62-63 0.850646237817152 1101 64-65 0.895161773647132 1101 66-67 0.8440473129342294 1101 68-69 0.8021513882536482 1101 70-71 0.8335680688606892 1101 72-73 0.7908252896702699 1101 74-75 0.627840900520436 1101 76-77 0.6845952045635464 1101 78-79 0.759786753652488 1101 80-81 0.9726879290513288 1101 82-83 0.9743256583094642 1101 84-85 1.013503380884302 1101 86-87 0.8316813174820936 1101 88-89 0.9088105535644928 1101 90-91 0.68622561651555 1101 92-93 0.8345882614205138 1101 94-95 0.8236987718424196 1101 96-97 0.7457965612291098 1101 98-99 0.6784374763214736 1101 100-101 1.3001429403879818 1102 1 0.2582252262960587 1102 2 0.2762886678520289 1102 3 0.2990826531503181 1102 4 0.298440829694691 1102 5 0.4415622374332884 1102 6 0.34707726874643896 1102 7 0.3288666008084675 1102 8 0.275900986832589 1102 9 0.40888772423932096 1102 10-11 0.45760482149378845 1102 12-13 0.39888443448219846 1102 14-15 0.46456339218076437 1102 16-17 0.5574203133824369 1102 18-19 0.5426715096554418 1102 20-21 0.59718795828271 1102 22-23 0.38650736186737333 1102 24-25 0.32634945799390636 1102 26-27 0.26916777925182345 1102 28-29 0.34435575872687707 1102 30-31 0.31067980223236447 1102 32-33 0.35603100662019216 1102 34-35 -0.018710795773941413 1102 36-37 0.17545903522287176 1102 38-39 0.2780100941342525 1102 40-41 0.22684124403336625 1102 42-43 0.239272305689461 1102 44-45 -0.0038192791060893683 1102 46-47 0.1691690213968613 1102 48-49 0.20659680463148078 1102 50-51 0.08350721156869412 1102 52-53 0.2593394975817347 1102 54-55 0.491634914315604 1102 56-57 0.4991961589444216 1102 58-59 0.43313858743213274 1102 60-61 0.407321391095266 1102 62-63 0.3717522639281512 1102 64-65 0.3792827723199039 1102 66-67 0.49920686187493857 1102 68-69 0.3424903015517913 1102 70-71 0.2804604043752086 1102 72-73 0.20896969208551042 1102 74-75 0.16296279627464116 1102 76-77 0.3258862337059263 1102 78-79 0.24373259858159813 1102 80-81 0.3385370384827162 1102 82-83 0.37611244286754797 1102 84-85 0.36235922340302196 1102 86-87 0.32036994497346427 1102 88-89 0.3696285312841603 1102 90-91 0.49472285581559206 1102 92-93 0.3871602410624355 1102 94-95 0.36187238890678586 1102 96-97 0.33272658671349475 1102 98-99 0.4474491223685355 1102 100-101 0.5455796931176344 1103 1 0.37423959540571516 1103 2 0.47458475313480974 1103 3 0.37117514865481027 1103 4 0.39791386421129005 1103 5 0.48947084747300806 1103 6 0.348181118634713 1103 7 0.3621011234807554 1103 8 0.3577157058829883 1103 9 0.5717312093594273 1103 10-11 0.5211485693288935 1103 12-13 0.5919821852397931 1103 14-15 0.5246860948805505 1103 16-17 0.7124496976673811 1103 18-19 0.6438940890616394 1103 20-21 0.7642696805553939 1103 22-23 0.6647958184715321 1103 24-25 0.6908784109154098 1103 26-27 0.4777402368204733 1103 28-29 0.2529230697966227 1103 30-31 0.26202627353962527 1103 32-33 0.4843052150468239 1103 34-35 0.37913151867650186 1103 36-37 0.5163070511681269 1103 38-39 0.5213693897543337 1103 40-41 0.3931313479407592 1103 42-43 0.45290755213316203 1103 44-45 0.4532384822960793 1103 46-47 0.4625668998744672 1103 48-49 0.31863883903190526 1103 50-51 0.4538298938730989 1103 52-53 0.6090141297791405 1103 54-55 0.5490813846380505 1103 56-57 0.6378842443652886 1103 58-59 0.5043348402726338 1103 60-61 0.36379270577053546 1103 62-63 0.46884461912171815 1103 64-65 0.4849175744280245 1103 66-67 0.49825481252531034 1103 68-69 0.491912384670286 1103 70-71 0.5555010601281118 1103 72-73 0.5668755456773589 1103 74-75 0.5852968330687212 1103 76-77 0.5544846080264669 1103 78-79 0.5492906691443373 1103 80-81 0.6038767555925908 1103 82-83 0.5259415335690854 1103 84-85 0.489156566665919 1103 86-87 0.5525875469951274 1103 88-89 0.6545262149678415 1103 90-91 0.5703719421055418 1103 92-93 0.5626160371373956 1103 94-95 0.5718404371966201 1103 96-97 0.4735867911777518 1103 98-99 0.5738684732891315 1103 100-101 0.9940264405673922 1104 1 0.22354426630353785 1104 2 0.3473241854529121 1104 3 0.28594211674633385 1104 4 0.37850126744516643 1104 5 0.5715300361813078 1104 6 0.20511593277822016 1104 7 0.44921129222357337 1104 8 0.36292343064305044 1104 9 0.5014668237560045 1104 10-11 0.5407293104369586 1104 12-13 0.39138646028594337 1104 14-15 0.5618193953449904 1104 16-17 0.3619290676865816 1104 18-19 0.6078836508128802 1104 20-21 0.7739883285228899 1104 22-23 0.4553981647025864 1104 24-25 0.5881977911182616 1104 26-27 0.3244362954980957 1104 28-29 0.5156818529635672 1104 30-31 0.6137319886423072 1104 32-33 0.44326232114020314 1104 34-35 0.2966932927228072 1104 36-37 0.28172621981310186 1104 38-39 0.44270491181401894 1104 40-41 0.4939110817027128 1104 42-43 0.5459022978599677 1104 44-45 0.5732740680986197 1104 46-47 0.5697042947011326 1104 48-49 0.7171827280300391 1104 50-51 0.6434682125436737 1104 52-53 0.7664048335819587 1104 54-55 0.9125519788525551 1104 56-57 0.8317869695385838 1104 58-59 0.8835753788285174 1104 60-61 0.8971940568150671 1104 62-63 1.0894083871509253 1104 64-65 0.8530059632537359 1104 66-67 0.9961382837465038 1104 68-69 0.862948524304187 1104 70-71 1.0673490614428722 1104 72-73 0.978076808754853 1104 74-75 0.657276385956763 1104 76-77 0.7820052769559567 1104 78-79 0.8442923173388728 1104 80-81 0.8771151111219275 1104 82-83 0.822004296057127 1104 84-85 0.9134844880313651 1104 86-87 0.9159417701255173 1104 88-89 1.0027873459807815 1104 90-91 1.0455136535869478 1104 92-93 1.0008329661377324 1104 94-95 0.8989666793985691 1104 96-97 1.0474577304206463 1104 98-99 1.221954715187092 1104 100-101 1.519792898526081 1105 1 0.25735725098249773 1105 2 0.3906482715237374 1105 3 0.3492037781507378 1105 4 0.3191052649590205 1105 5 0.4084564271761266 1105 6 0.3003138178526257 1105 7 0.4539824324214692 1105 8 0.4868916878367173 1105 9 0.5977802720882579 1105 10-11 0.5616957929002169 1105 12-13 0.4431711841007129 1105 14-15 0.2145244265933428 1105 16-17 0.27307960411059184 1105 18-19 0.20138932232745077 1105 20-21 0.44026477382829654 1105 22-23 0.3086906298877281 1105 24-25 0.41875398906881145 1105 26-27 0.44453152907775717 1105 28-29 0.4051405650277218 1105 30-31 0.2555714595625673 1105 32-33 0.29957178501078374 1105 34-35 0.03532986283455131 1105 36-37 0.2571053968185666 1105 38-39 0.24298102877227024 1105 40-41 0.12885074656712447 1105 42-43 0.21792234574446923 1105 44-45 0.13248412545616617 1105 46-47 0.2489913620480948 1105 48-49 0.3059516100079449 1105 50-51 0.39687013753538025 1105 52-53 0.17411267040309042 1105 54-55 0.16618710129088754 1105 56-57 0.4666936276643554 1105 58-59 0.45172816583903597 1105 60-61 0.22968571831300721 1105 62-63 0.25038203648453816 1105 64-65 0.35407389789523336 1105 66-67 0.41428329213311343 1105 68-69 0.5544421716896508 1105 70-71 0.6643960764779244 1105 72-73 0.6431895783739527 1105 74-75 0.6248430722016955 1105 76-77 0.45434533968596114 1105 78-79 0.5753163186135559 1105 80-81 0.49357213124130794 1105 82-83 0.5635017010501322 1105 84-85 0.723171826055097 1105 86-87 0.8246535708731884 1105 88-89 0.6128439079193235 1105 90-91 0.6762151779499561 1105 92-93 0.6229022607213679 1105 94-95 0.587440102684404 1105 96-97 0.7271506623877393 1105 98-99 0.7892506171585829 1105 100-101 1.2383326115996844 1106 1 0.05449935945618023 1106 2 0.067091485205939 1106 3 0.194381176874181 1106 4 0.19265411659520737 1106 5 0.15576226239231517 1106 6 0.1499074081476266 1106 7 0.27631386284480186 1106 8 0.2315249526557679 1106 9 0.35752447402333587 1106 10-11 0.34446273578903686 1106 12-13 0.41031897965421393 1106 14-15 0.49653662613842187 1106 16-17 0.6062129150199951 1106 18-19 0.5379500027771797 1106 20-21 0.381346582292899 1106 22-23 0.30538467994787055 1106 24-25 0.20391980605867133 1106 26-27 0.07322854945415003 1106 28-29 0.3441870885993126 1106 30-31 0.3471533650066121 1106 32-33 0.07979845799543739 1106 34-35 0.17010227139097367 1106 36-37 0.08336689401597397 1106 38-39 0.2078006481262875 1106 40-41 0.17526785280325186 1106 42-43 0.08098787594748558 1106 44-45 0.121796801238375 1106 46-47 0.24369504577146728 1106 48-49 0.38906449831909384 1106 50-51 0.18230479626929252 1106 52-53 0.4409803415639999 1106 54-55 0.4770178747315086 1106 56-57 0.2515194028135568 1106 58-59 0.34639236587246813 1106 60-61 0.3977920479569548 1106 62-63 0.49898297690612736 1106 64-65 0.36131036100476877 1106 66-67 0.39675858246322804 1106 68-69 0.38262423011346414 1106 70-71 0.5121885721662984 1106 72-73 0.6401039246577405 1106 74-75 0.40511332455581694 1106 76-77 0.5869633315262952 1106 78-79 0.7179905429063602 1106 80-81 0.717729286147943 1106 82-83 0.8359739246425413 1106 84-85 0.6930808015303462 1106 86-87 0.8114219591909304 1106 88-89 0.8504688177753152 1106 90-91 0.978045221310591 1106 92-93 0.9785693420151418 1106 94-95 1.1577016525565007 1106 96-97 1.4015689140749465 1106 98-99 1.5915959684283898 1106 100-101 1.7895337951729502 1107 1 0.1972062889895163 1107 2 0.2517250197561438 1107 3 0.10867284653118858 1107 4 0.25552220569059614 1107 5 0.3854148598265539 1107 6 0.29680126115387395 1107 7 0.3434362135114952 1107 8 0.4424303133886198 1107 9 0.42224546835895893 1107 10-11 0.37419516431097577 1107 12-13 0.38722422697552616 1107 14-15 0.315258367395316 1107 16-17 0.2667497104176064 1107 18-19 0.44987819378346927 1107 20-21 0.587418773164508 1107 22-23 0.5870902919581482 1107 24-25 0.5829088131141091 1107 26-27 0.41712000929982906 1107 28-29 0.5222467412942038 1107 30-31 0.5069279551523849 1107 32-33 0.3310439913087464 1107 34-35 0.4179081347672877 1107 36-37 0.4506593398331731 1107 38-39 0.37911585865087716 1107 40-41 0.33902081767313774 1107 42-43 0.5472025356287062 1107 44-45 0.6204240122095257 1107 46-47 0.526792607157681 1107 48-49 0.6758876950535537 1107 50-51 0.5469596486438064 1107 52-53 0.5309225548432437 1107 54-55 0.5592992717844041 1107 56-57 0.6566394684150438 1107 58-59 0.6261579096763441 1107 60-61 0.6615139575591087 1107 62-63 0.8141737873821668 1107 64-65 0.81272404020784 1107 66-67 0.6843478608203171 1107 68-69 0.7133784478263934 1107 70-71 0.8457227015068476 1107 72-73 0.738364275896803 1107 74-75 0.7276601577602335 1107 76-77 0.7049336992188948 1107 78-79 0.7102002558954332 1107 80-81 0.7860597354614036 1107 82-83 0.9524400389595975 1107 84-85 1.0110657000757541 1107 86-87 0.891442435289008 1107 88-89 0.9319799245744242 1107 90-91 0.9588553030643574 1107 92-93 1.0830358736511982 1107 94-95 1.0365294025868472 1107 96-97 1.110942111562526 1107 98-99 1.2262584317800709 1107 100-101 1.5357232119275537 1108 1 -0.009609698472864636 1108 2 0.15507376588077548 1108 3 0.04025180945817297 1108 4 0.18948733157839825 1108 5 0.2210614762208749 1108 6 0.02406159543271258 1108 7 -0.12749277895401434 1108 8 9.651522546860747E-4 1108 9 0.1854668599852971 1108 10-11 -0.11812709173237579 1108 12-13 0.11984833996688593 1108 14-15 0.17698069565912533 1108 16-17 0.27339082022953676 1108 18-19 0.17743925713417497 1108 20-21 0.20330139536739011 1108 22-23 0.4001468681354936 1108 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-2.682093667086537 2316 72-73 -2.224470322780455 2316 74-75 -1.8326548411309567 2316 76-77 -1.8916172602248764 2316 78-79 -2.7006109339191973 2316 80-81 -2.2502667232714266 2316 82-83 -1.9970807283345238 2316 84-85 -2.0073755462227894 2316 86-87 -2.673554825306617 2316 88-89 -2.944872434574531 2316 90-91 -2.890844340813075 2316 92-93 -3.4938517064111565 2316 94-95 -3.889677892055662 2316 96-97 -4.962813905343513 2316 98-99 -6.124638070913676 2316 100-101 -6.485884655380918 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8381.0 3 3389.0 4 3435.0 5 3692.0 6 3869.0 7 4456.0 8 5480.0 9 6743.0 10 8216.0 11 10186.0 12 11985.0 13 14216.0 14 15936.0 15 18810.0 16 22270.0 17 27478.0 18 24000.0 19 18371.0 20 18885.0 21 20380.0 22 22646.0 23 25408.0 24 29022.0 25 33397.0 26 39227.0 27 46265.0 28 54732.0 29 66313.0 30 80522.0 31 98858.0 32 122525.0 33 157124.0 34 206363.0 35 277761.0 36 383628.0 37 493407.0 38 376579.0 39 53699.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.19106795509872 18.420616332896305 14.395810501225345 22.992505210779633 2 15.633385385808952 19.765788708600443 40.183363394555045 24.417462511035563 3 19.486368317230944 24.26848822378657 29.324438061153458 26.920705397829025 4 12.871949465790342 16.359243123093695 36.99578526511641 33.773022145999555 5 14.02036252278817 37.19516049193333 33.67624781883377 15.108229166444733 6 32.20160908627064 35.57906405418869 17.42612238898075 14.793204470559914 7 29.208087606864165 32.48361596685591 20.20545330785791 18.10284311842202 8 26.789988801807617 34.16219129523392 19.54102323216615 19.50679667079232 9 26.69716995434448 16.853557590607153 20.218765877266577 36.23050657778178 10-11 25.276559209121174 26.390874136421022 26.938284628955532 21.394282025502267 12-13 26.4289836825293 23.724146281145938 27.404999683105924 22.44187035321884 14-15 23.1116161385743 25.14934396234283 26.016161154965694 25.72287874411718 16-17 22.373166921137663 28.600592976600502 26.468575790931364 22.55766431133047 18-19 22.2942352948487 26.944206488633306 28.92772524995717 21.833832966560824 20-21 23.261133574139205 25.971042600206474 28.852744542690985 21.915079282963344 22-23 22.72603515806647 26.084055765029785 28.796910730373654 22.392998346530092 24-25 22.74776545389143 26.20442421229887 28.917148192638308 22.13066214117139 26-27 22.43407440087925 26.41579584293956 28.8830178325793 22.267111923601888 28-29 22.0933340195172 26.590878841816277 29.053509919201847 22.26227721946467 30-31 22.309983681893648 26.204270037596423 29.03643312282769 22.449313157682244 32-33 22.116240338518953 26.45455698931791 28.908659686058986 22.520542986104147 34-35 22.845963659698494 26.400774025564704 28.51406470609531 22.239197608641486 36-37 22.856500933831093 26.017719720056533 28.604939402135138 22.520839943977233 38-39 22.473837759770056 26.249520164451155 28.817640840052512 22.459001235726276 40-41 22.621775139066763 26.170300020779933 28.8613463518787 22.346578488274595 42-43 22.611009971719024 26.255257764668993 28.40405824990147 22.729674013710515 44-45 22.592042350696477 26.52128608014719 28.228953129170193 22.657718439986144 46-47 22.826013660990792 26.65832758979266 27.84134909204737 22.674309657169182 48-49 22.939757368513984 26.718264083779108 27.621038472697524 22.720940075009384 50-51 22.7386941807114 26.82369154022623 27.646731476854587 22.790882802207786 52-53 22.999128686446017 26.940092313097917 27.38975044231141 22.671028558144652 54-55 22.737965689980356 26.64918299505304 27.54538975526409 23.067461559702515 56-57 22.850269325311253 27.069563133934988 27.38318735635244 22.696980184401315 58-59 22.944887250176404 26.73598737554075 27.401337692926926 22.91778768135592 60-61 22.889572619721367 26.603902802793584 27.945856184750014 22.560668392735035 62-63 22.72776397227842 26.8208544652711 27.746226153422704 22.70515540902777 64-65 22.583458052087664 26.867809004096642 27.618978497504855 22.929754446310838 66-67 22.575814713693365 27.09354378162736 27.38023639940337 22.950405105275905 68-69 22.48438522733888 26.863390084637107 27.608739019254553 23.043485668769463 70-71 22.60956336145328 26.810998421125575 27.541216750159315 23.038221467261824 72-73 22.716443903717572 26.723018278102355 27.636559611854956 22.92397820632512 74-75 22.472340995662048 27.233371989540643 27.29743422751322 22.996852787284087 76-77 22.648815550155554 26.9994972474609 27.320722934371183 23.030964268012358 78-79 22.736833026740154 27.010782165753394 27.287461362338476 22.964923445167972 80-81 22.523978557634255 27.354597994685193 27.248880444578056 22.872543003102493 82-83 22.71760591699133 27.215917016177034 27.211817656209575 22.854659410622062 84-85 22.639378318346512 27.112937591347432 27.117232639884143 23.13045145042192 86-87 22.76881098370361 27.002568893040635 27.167357099557204 23.06126302369855 88-89 22.772212568287394 26.92494846502436 27.060369745703998 23.242469220984248 90-91 22.626926748936143 27.00044719385854 27.28750306114844 23.085122996056885 92-93 22.76611396802719 26.85377075947626 27.35631979909397 23.02379547340258 94-95 22.53353579215742 26.867073926930964 27.276428348068745 23.322961932842876 96-97 22.57007445077645 26.87351460478904 27.216663969174764 23.33974697525974 98-99 22.580417863887984 26.867288094711128 26.974383531227975 23.577910510172913 100-101 22.497327086980132 26.798325051430165 27.138654378456085 23.56569348313362 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 233.0 1 197.5 2 247.0 3 380.0 4 375.0 5 353.0 6 638.0 7 1295.0 8 2122.0 9 2648.5 10 2504.5 11 2219.0 12 2284.5 13 2476.5 14 2688.0 15 2883.0 16 3081.0 17 3284.0 18 3467.5 19 3747.5 20 4229.0 21 4894.0 22 5767.0 23 7020.0 24 8649.5 25 10834.0 26 13648.0 27 17019.0 28 20388.0 29 24236.5 30 29819.0 31 35588.5 32 41420.5 33 46513.0 34 52335.5 35 57776.0 36 62872.0 37 68331.5 38 70917.0 39 72940.0 40 76620.0 41 83612.5 42 90438.5 43 96151.5 44 100576.0 45 104503.0 46 106016.0 47 108643.0 48 119359.5 49 119248.0 50 108993.5 51 102874.0 52 98809.5 53 97507.0 54 95065.5 55 88614.5 56 81462.5 57 73545.5 58 66736.5 59 59889.0 60 50867.5 61 41750.0 62 34093.0 63 26933.5 64 20645.0 65 16532.0 66 12848.0 67 10332.5 68 7875.5 69 5469.5 70 4191.5 71 3393.0 72 2775.0 73 2385.5 74 1944.0 75 1567.0 76 1130.5 77 697.0 78 428.0 79 276.0 80 201.0 81 140.0 82 99.5 83 68.0 84 37.5 85 24.5 86 16.5 87 15.5 88 13.5 89 9.5 90 8.0 91 6.0 92 5.0 93 4.5 94 3.5 95 3.0 96 4.5 97 3.5 98 2.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0325090952711688 2 0.02285573947012304 3 0.021152206093467907 4 0.04837324984126973 5 0.05390973331539891 6 0.045853440054967345 7 0.04840874011995004 8 0.040707349646321625 9 0.04067185936764131 10-11 0.05016550891462565 12-13 0.04523236017806183 14-15 0.03919901280240823 16-17 0.04872815262807288 18-19 0.051123746438994165 20-21 0.011623066267803255 22-23 0.020122988011738764 24-25 0.005483248056108711 26-27 0.00970659121906623 28-29 0.005607464031489814 30-31 0.008890314809418978 32-33 0.0037442244007732622 34-35 0.00692060434266148 36-37 0.008766098834037875 38-39 0.010700319022115058 40-41 0.0018277493520362368 42-43 0.0019342201880771827 44-45 0.002022945884777971 46-47 5.323541802047293E-4 48-49 9.050021063480397E-4 50-51 3.194125081228375E-4 52-53 0.0031231445238677448 54-55 0.006636682113218958 56-57 0.015899644848781248 58-59 0.010114729423889857 60-61 0.010203455120590644 62-63 0.005554228613469342 64-65 0.0 66-67 0.006938349482001638 68-69 0.016644940701067867 70-71 0.0056606994495102874 72-73 0.0023601035322409663 74-75 0.003566773007371686 76-77 0.006512466137837855 78-79 0.005323541802047292 80-81 0.009227472456881974 82-83 0.0056784445888504445 84-85 0.01687562751248992 86-87 0.004152362605596888 88-89 0.005997857096973282 90-91 0.0036554987040724736 92-93 0.015456016365277304 94-95 0.011001986390897738 96-97 0.008553157161955982 98-99 0.019856810921636398 100-101 0.03511763075417197 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2817673.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.77571236903658 #Duplication Level Percentage of deduplicated Percentage of total 1 76.21119857349335 39.45889096640723 2 13.176226909209813 13.644170691208146 3 4.47085568314504 6.944452136819707 4 2.0083366965974747 4.159322525728477 5 1.062437825620277 2.750423763465006 6 0.6152244290226881 1.911220984769008 7 0.434422964779299 1.574479092964197 8 0.2904852097552463 1.2032062934197512 9 0.23063668858400335 1.074724096488518 >10 1.2917785042497747 12.683053715974618 >50 0.1295925130361749 4.666008148447579 >100 0.07391662418419886 7.085472984281771 >500 0.0037171609777998053 1.3038717648510618 >1k 0.001032544716038653 0.9243361639238374 >5k 1.3767262880515376E-4 0.6163666712511604 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9658 0.342765111494485 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7636 0.2710037680028875 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4619 0.16392959722437628 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3275 0.11623066267803255 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.549027868031528E-5 0.0 6 0.0 0.0 0.0 7.098055736063056E-5 0.0 7 0.0 0.0 0.0 7.098055736063056E-5 0.0 8 0.0 0.0 0.0 7.098055736063056E-5 0.0 9 0.0 0.0 0.0 1.0647083604094584E-4 3.549027868031528E-5 10-11 0.0 0.0 0.0 1.0647083604094584E-4 3.549027868031528E-5 12-13 0.0 0.0 0.0 1.0647083604094584E-4 3.549027868031528E-5 14-15 0.0 0.0 0.0 1.0647083604094584E-4 3.549027868031528E-5 16-17 0.0 0.0 0.0 1.2421597538110347E-4 3.549027868031528E-5 18-19 0.0 0.0 0.0 1.4196111472126113E-4 3.549027868031528E-5 20-21 0.0 0.0 0.0 1.4196111472126113E-4 3.549027868031528E-5 22-23 0.0 0.0 0.0 1.4196111472126113E-4 7.098055736063056E-5 24-25 0.0 0.0 0.0 1.7745139340157642E-4 1.0647083604094584E-4 26-27 0.0 0.0 0.0 2.4843195076220694E-4 1.0647083604094584E-4 28-29 0.0 0.0 0.0 6.388250162456751E-4 1.0647083604094584E-4 30-31 0.0 0.0 0.0 0.0014728465652330842 1.0647083604094584E-4 32-33 0.0 0.0 0.0 0.002626280622343331 1.0647083604094584E-4 34-35 0.0 0.0 0.0 0.004152362605596888 1.0647083604094584E-4 36-37 0.0 0.0 0.0 0.00631726960509612 1.0647083604094584E-4 38-39 0.0 0.0 0.0 0.009138746760181185 1.0647083604094584E-4 40-41 0.0 0.0 0.0 0.011552085710442625 1.0647083604094584E-4 42-43 0.0 0.0 0.0 0.014568759398269423 1.4196111472126113E-4 44-45 0.0 0.0 0.0 0.01790484559421906 1.7745139340157642E-4 46-47 0.0 0.0 0.0 0.023015445724184458 1.7745139340157642E-4 48-49 0.0 0.0 0.0 0.02947467644400184 1.7745139340157642E-4 50-51 0.0 0.0 0.0 0.03579194604909797 1.7745139340157642E-4 52-53 0.0 0.0 0.0 0.046900403276036645 1.7745139340157642E-4 54-55 0.0 0.0 0.0 0.06704113642711557 1.7745139340157642E-4 56-57 0.0 0.0 0.0 0.10065043033737414 1.7745139340157642E-4 58-59 0.0 0.0 0.0 0.13823463545982803 1.9519653274173405E-4 60-61 0.0 0.0 0.0 0.17734492256553547 2.1294167208189168E-4 62-63 0.0 0.0 0.0 0.2221513993994335 2.1294167208189168E-4 64-65 0.0 0.0 0.0 0.27717907649326234 2.1294167208189168E-4 66-67 0.0 0.0 0.0 0.3567837715732095 2.3068681142204934E-4 68-69 0.0 0.0 0.0 0.45432880252605606 2.48431950762207E-4 70-71 0.0 0.0 0.0 0.5458759763819294 2.48431950762207E-4 72-73 0.0 0.0 0.0 0.6380619752540483 3.194125081228375E-4 74-75 0.0 0.0 0.0 0.7408950577302618 3.194125081228375E-4 76-77 0.0 0.0 0.0 0.8626444587430834 3.194125081228375E-4 78-79 0.0 0.0 0.0 0.9756809963398876 3.194125081228375E-4 80-81 0.0 0.0 0.0 1.0993823626801267 3.194125081228375E-4 82-83 0.0 0.0 0.0 1.237333075910512 3.194125081228375E-4 84-85 0.0 0.0 0.0 1.4186174194095624 3.194125081228375E-4 86-87 0.0 0.0 0.0 1.6394911687765046 3.194125081228375E-4 88-89 3.549027868031528E-5 0.0 0.0 1.8940806828897463 3.3715764746299515E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15985 0.0 27.764837 1 GGTATCA 11450 0.0 27.34896 1 GTACATG 13305 0.0 24.214512 1 ATCAACG 18905 0.0 23.275293 3 TCAACGC 19065 0.0 23.154736 4 TACATGG 13495 0.0 23.028505 2 TATCAAC 19160 0.0 22.940723 2 CAACGCA 19375 0.0 22.783855 5 AACGCAG 19670 0.0 22.416803 6 ACATGGG 13490 0.0 22.297318 3 GAGTACT 9180 0.0 22.154503 12-13 AGTACTT 9710 0.0 20.333527 12-13 GTACTTT 9940 0.0 20.197668 14-15 ACGCAGA 21835 0.0 20.107786 7 CGCAGAG 22100 0.0 19.844116 8 CATGGGG 6120 0.0 18.634626 4 GCAGAGT 23005 0.0 18.629404 9 GTATAGG 770 0.0 18.513624 1 AGAGTAC 18270 0.0 18.153744 10-11 TATGACG 395 1.8189894E-11 18.044924 4 >>END_MODULE