FastQCFastQC Report
Thu 26 May 2016
SRR936405_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936405_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2817673
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102750.3646626134402395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93950.33343116820156204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56220.19952634674073252No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37710.13383384090346892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122450.029.842341
GTATCAA171100.028.4390621
GTACATG130350.025.0444221
TACATGG134400.023.9975132
ATCAACG204900.023.5530683
TCAACGC206150.023.477714
TATCAAC209400.023.1149652
ACATGGG133000.023.071543
CAACGCA209950.023.0301515
AACGCAG214300.022.5848356
GAGTACT116600.022.09984412-13
GTACTTT124900.020.46010414-15
ACGCAGA238350.020.2661237
CGCAGAG241400.020.0100698
AGTACTT124150.019.60808412-13
CATGGGA74950.018.9480484
GCAGAGT254150.018.913119
CATGGGG60150.018.7934364
AGAGTAC203100.018.70975310-11
ACTTTTT145600.017.68146316-17
Produced by FastQC (version 0.11.5)