##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936405_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2817673 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.75601675567037 31.0 31.0 34.0 30.0 34.0 2 31.90419825153593 31.0 31.0 34.0 30.0 34.0 3 32.00794627339652 31.0 31.0 34.0 30.0 34.0 4 35.77643076396729 37.0 35.0 37.0 35.0 37.0 5 35.5356412898161 37.0 35.0 37.0 33.0 37.0 6 35.638030388906024 37.0 35.0 37.0 33.0 37.0 7 35.5785596838242 37.0 35.0 37.0 33.0 37.0 8 35.55307304999551 37.0 35.0 37.0 33.0 37.0 9 37.15980598174451 39.0 37.0 39.0 33.0 39.0 10-11 37.02078949544536 39.0 37.0 39.0 33.0 39.0 12-13 36.91786307353621 39.0 37.0 39.0 33.0 39.0 14-15 37.98318701282938 40.0 37.0 41.0 33.0 41.0 16-17 37.90338091041792 40.0 37.0 41.0 33.0 41.0 18-19 38.060497616295436 40.0 37.0 41.0 33.0 41.0 20-21 38.093972579500885 40.0 37.0 41.0 33.0 41.0 22-23 37.99975848865358 40.0 37.0 41.0 33.0 41.0 24-25 37.872013359960505 40.0 37.0 41.0 33.0 41.0 26-27 37.6522144336834 39.0 37.0 41.0 32.0 41.0 28-29 37.56395898317513 39.0 36.5 41.0 32.0 41.0 30-31 37.535190208374075 39.0 37.0 41.0 32.0 41.0 32-33 37.388896262980126 39.0 36.0 41.0 32.0 41.0 34-35 37.22440272522752 39.0 36.0 41.0 31.0 41.0 36-37 36.968180125940805 39.0 36.0 41.0 31.0 41.0 38-39 36.807075909802165 39.0 35.0 41.0 30.0 41.0 40-41 36.58409474768719 39.0 35.0 40.0 30.0 41.0 42-43 36.32930311643686 39.0 35.0 40.0 30.0 41.0 44-45 36.08509592844876 38.5 35.0 40.0 30.0 41.0 46-47 35.73687507386414 38.0 35.0 40.0 28.5 41.0 48-49 35.38030122728932 38.0 34.5 40.0 27.0 41.0 50-51 35.05487666595805 38.0 34.0 40.0 26.5 41.0 52-53 34.76568182326338 38.0 34.0 40.0 26.0 41.0 54-55 34.482077586717836 38.0 33.5 40.0 25.5 41.0 56-57 34.15538016654168 37.5 33.0 40.0 24.5 41.0 58-59 33.80627542656653 37.0 33.0 40.0 23.5 41.0 60-61 33.4426184301727 36.5 32.0 39.0 23.0 40.5 62-63 33.028662658867795 36.0 32.0 39.0 22.0 40.0 64-65 32.59570663451721 35.5 31.0 39.0 21.5 40.0 66-67 32.11635026491719 35.0 31.0 38.0 20.0 40.0 68-69 31.815692772014355 35.0 31.0 38.0 19.5 40.0 70-71 31.374876538193043 35.0 30.0 37.0 18.0 39.5 72-73 30.906540609928832 34.0 30.0 37.0 16.5 39.0 74-75 30.365493085961358 34.0 29.0 36.0 12.5 39.0 76-77 28.978834130149238 32.5 28.0 35.0 11.0 37.0 78-79 29.760991605484385 34.0 29.0 35.0 9.0 37.0 80-81 29.555846615274376 34.0 29.0 35.0 7.0 37.0 82-83 29.208664738598127 34.0 29.0 35.0 6.0 36.5 84-85 28.771626090039547 33.5 29.0 35.0 2.0 36.0 86-87 28.354138326200378 33.0 27.5 35.0 2.0 35.5 88-89 27.920562641584027 33.0 27.0 35.0 2.0 35.0 90-91 27.409798085157504 33.0 26.0 35.0 2.0 35.0 92-93 26.870620011619515 32.0 25.0 34.0 2.0 35.0 94-95 26.22035718836075 32.0 24.5 34.0 2.0 35.0 96-97 25.373688501114216 32.0 21.5 34.0 2.0 35.0 98-99 24.310360712545425 31.0 10.0 34.0 2.0 35.0 100-101 20.996433404444023 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1938079931190586 1101 2 0.3231626319845695 1101 3 0.26207045751694835 1101 4 0.2097532029034923 1101 5 0.1059267959399719 1101 6 0.08655196752468441 1101 7 0.08599112679624454 1101 8 0.08679774049716116 1101 9 0.20641433329245018 1101 10-11 0.21939030963972783 1101 12-13 0.2377940230037936 1101 14-15 0.30968324277903747 1101 16-17 0.3319362314069991 1101 18-19 0.33204762565702595 1101 20-21 0.3385443401777408 1101 22-23 0.20254937287171515 1101 24-25 0.1429930025249888 1101 26-27 0.14913168449353975 1101 28-29 0.12945913798512976 1101 30-31 0.2347587627303369 1101 32-33 0.26680563627741805 1101 34-35 0.2434611877582995 1101 36-37 0.15660393369506664 1101 38-39 0.3328988612291752 1101 40-41 0.3514474939025618 1101 42-43 0.5258920051914231 1101 44-45 0.42474136048453914 1101 46-47 0.41119822421640606 1101 48-49 0.44811390119163974 1101 50-51 0.47685921098712214 1101 52-53 0.4968834454159108 1101 54-55 0.4896406821640227 1101 56-57 0.4708481731940424 1101 58-59 0.5275860477833376 1101 60-61 0.33458317811226834 1101 62-63 0.4745558372669407 1101 64-65 0.4097684439902807 1101 66-67 0.316136044241766 1101 68-69 0.29096040675388934 1101 70-71 0.29210936146196786 1101 72-73 0.32375527838865636 1101 74-75 0.3931584511816766 1101 76-77 0.31355508088262596 1101 78-79 0.12961716982075444 1101 80-81 0.2924279787907764 1101 82-83 0.45365446125365594 1101 84-85 0.43582695008214145 1101 86-87 0.35157936830243486 1101 88-89 0.24213877409788864 1101 90-91 0.29049051833201034 1101 92-93 0.10357979657468164 1101 94-95 0.34996502072062796 1101 96-97 0.5194018023804752 1101 98-99 0.5547379204721814 1101 100-101 0.8409503955539108 1102 1 0.25556748441806576 1102 2 0.32885777921973514 1102 3 0.30540505158219133 1102 4 0.17779224260659277 1102 5 0.16100902407734452 1102 6 0.21406713850027614 1102 7 0.22722227477919432 1102 8 0.20034691574913666 1102 9 0.32460805787676605 1102 10-11 0.31253769799895537 1102 12-13 0.2352624617921606 1102 14-15 0.4070032174028384 1102 16-17 0.40878880410067353 1102 18-19 0.2720048357813738 1102 20-21 0.18625145975008195 1102 22-23 0.07945393993796301 1102 24-25 0.2309439828450195 1102 26-27 0.06324490023838081 1102 28-29 0.03403026122369823 1102 30-31 0.25337758404408817 1102 32-33 0.3254047330509948 1102 34-35 0.23208685077850788 1102 36-37 0.39847513391905665 1102 38-39 0.4072215496000524 1102 40-41 0.5099173295934136 1102 42-43 0.4837551425011384 1102 44-45 0.5505521091905194 1102 46-47 0.5425496841050119 1102 48-49 0.45615885652581056 1102 50-51 0.35542914504103607 1102 52-53 0.3659388744895722 1102 54-55 0.44131994028243327 1102 56-57 0.41591037386938723 1102 58-59 0.2845194962846662 1102 60-61 0.4334755876262619 1102 62-63 0.42389935151530267 1102 64-65 0.44474455860206064 1102 66-67 0.37101758665061624 1102 68-69 0.15648409156456466 1102 70-71 0.33927391553719843 1102 72-73 0.33109393896342 1102 74-75 0.2046778769110844 1102 76-77 -0.005934922047057256 1102 78-79 0.04277834545181847 1102 80-81 0.4798966858813536 1102 82-83 0.36607374880742327 1102 84-85 0.2690347150944099 1102 86-87 0.3260355911179005 1102 88-89 0.23813111796438946 1102 90-91 0.3972585174076677 1102 92-93 0.3779793405474656 1102 94-95 0.48028243522201564 1102 96-97 0.6539556872017975 1102 98-99 0.7534121397019184 1102 100-101 1.1441511544164307 1103 1 0.1330689502701432 1103 2 0.25191126690991794 1103 3 0.21954683635624406 1103 4 0.15976435345370987 1103 5 0.08656714123389975 1103 6 0.17359416042573628 1103 7 0.12374799620767618 1103 8 0.24043041186961744 1103 9 0.25319382009813296 1103 10-11 0.09005650945845645 1103 12-13 0.19882133143885738 1103 14-15 0.13347119532541285 1103 16-17 0.3540236491174582 1103 18-19 0.24172166079818425 1103 20-21 0.30719979641963846 1103 22-23 0.31783076807020905 1103 24-25 0.22387310634225344 1103 26-27 0.4015306798159486 1103 28-29 0.4260719631136993 1103 30-31 0.30478004205856024 1103 32-33 0.26290722820285595 1103 34-35 0.24335440063098446 1103 36-37 0.38685585369398723 1103 38-39 0.4336340491116033 1103 40-41 0.40339989233384443 1103 42-43 0.33266685388886685 1103 44-45 0.38093287704180767 1103 46-47 0.3804686962144288 1103 48-49 0.30091514068158887 1103 50-51 0.29994146820558143 1103 52-53 0.252898885240036 1103 54-55 0.16785950325149201 1103 56-57 0.1167712370007905 1103 58-59 0.21888469205865135 1103 60-61 0.2534335303525097 1103 62-63 0.23522907234526969 1103 64-65 0.09764990019139219 1103 66-67 0.2396557436194584 1103 68-69 0.2951475034413136 1103 70-71 0.29595982650933195 1103 72-73 0.12752549852307737 1103 74-75 0.05399993157558214 1103 76-77 -0.13294749697706365 1103 78-79 -0.06952493201023557 1103 80-81 0.2718607455286417 1103 82-83 0.3772984111690292 1103 84-85 0.15258453495106394 1103 86-87 -0.10448540294424191 1103 88-89 -0.013745527445966133 1103 90-91 0.13718384869764932 1103 92-93 0.24463810744421366 1103 94-95 0.4049721601098817 1103 96-97 0.25314868228731413 1103 98-99 0.4678921638205722 1103 100-101 0.9430749007753612 1104 1 0.13277351021169892 1104 2 0.24194040242950265 1104 3 0.11521184722862898 1104 4 0.10827798596997695 1104 5 0.012346138770631399 1104 6 0.1410235564265605 1104 7 0.13164508047079693 1104 8 0.02557519174777667 1104 9 0.07265239531707834 1104 10-11 0.13019835868522733 1104 12-13 0.0554915794850146 1104 14-15 0.1715682383599244 1104 16-17 0.2498862533408328 1104 18-19 0.16147643604999473 1104 20-21 0.13434408801422393 1104 22-23 0.18190839185817254 1104 24-25 0.13376246356538246 1104 26-27 0.20784359738627245 1104 28-29 0.1907050332108895 1104 30-31 0.11803010119407276 1104 32-33 0.28552169222614054 1104 34-35 0.2615733625629062 1104 36-37 0.43247236869825656 1104 38-39 0.33807676461422886 1104 40-41 0.4113596744871728 1104 42-43 0.35648350245864435 1104 44-45 0.38577296417286533 1104 46-47 0.3215934773655462 1104 48-49 0.3182591820545966 1104 50-51 0.3091024849373696 1104 52-53 0.3178823985448105 1104 54-55 0.40680021289661283 1104 56-57 0.3101272555707695 1104 58-59 0.35992977089174616 1104 60-61 0.4784420088172112 1104 62-63 0.26133419390792767 1104 64-65 0.33563114212260103 1104 66-67 0.14610241668891177 1104 68-69 0.3957301188362976 1104 70-71 0.2889139136043042 1104 72-73 0.3138650531970626 1104 74-75 0.3877968493093036 1104 76-77 0.3307599242682109 1104 78-79 0.3395303532794145 1104 80-81 0.5624858909924981 1104 82-83 0.46080630112853527 1104 84-85 0.4921824324818118 1104 86-87 0.5017599718988919 1104 88-89 0.5563241147105771 1104 90-91 0.7502181700927331 1104 92-93 0.7559451019638459 1104 94-95 0.6580550438708386 1104 96-97 0.8439770259135493 1104 98-99 0.8605391262502877 1104 100-101 1.3135320348927984 1105 1 0.11133117164018813 1105 2 0.14757972736751768 1105 3 0.09664604055414827 1105 4 0.09129349236376783 1105 5 0.029679084624206098 1105 6 0.07742838719391187 1105 7 0.06807840239691387 1105 8 0.0931879369074764 1105 9 0.01715290821169191 1105 10-11 -0.010515120137938538 1105 12-13 -0.08707495355273664 1105 14-15 0.14588200729834 1105 16-17 0.17875425874791517 1105 18-19 0.08016530302757019 1105 20-21 0.1862592798333722 1105 22-23 0.16744859121672562 1105 24-25 0.11408233004183899 1105 26-27 0.20864565038391447 1105 28-29 0.19895155720888624 1105 30-31 0.18850308990910492 1105 32-33 0.2436129179061126 1105 34-35 0.2792627166855439 1105 36-37 0.034460341988818755 1105 38-39 0.16094862826177803 1105 40-41 0.42521234466244806 1105 42-43 0.3192945842507413 1105 44-45 0.30511852548148966 1105 46-47 0.28917682825050406 1105 48-49 0.2100441165964284 1105 50-51 0.2112759948040761 1105 52-53 0.2876588160579061 1105 54-55 0.3483890906363172 1105 56-57 0.25113636510808846 1105 58-59 0.42110571820396814 1105 60-61 0.37211063900852537 1105 62-63 0.4369319787364603 1105 64-65 0.31319193553579083 1105 66-67 0.4831330960761875 1105 68-69 0.5026387840298128 1105 70-71 0.4174526364757476 1105 72-73 0.5104791531382418 1105 74-75 0.28318935587651595 1105 76-77 0.22011461672442678 1105 78-79 0.4610425113169718 1105 80-81 0.48331573415887874 1105 82-83 0.4809386377228684 1105 84-85 0.44291960627282734 1105 86-87 0.46189637896594604 1105 88-89 0.59472740136464 1105 90-91 0.5255846819717753 1105 92-93 0.4814330560631923 1105 94-95 0.5327159318854271 1105 96-97 0.7395469302617421 1105 98-99 1.009274586486253 1105 100-101 1.144209508417255 1106 1 0.04563792263693145 1106 2 0.13276954928022988 1106 3 0.11179447489973882 1106 4 0.09836462170666493 1106 5 0.010134769706404256 1106 6 0.05078571280222377 1106 7 0.08629491363235786 1106 8 0.08415969769925624 1106 9 0.16163140767969963 1106 10-11 0.061303624950312496 1106 12-13 0.07586869949432185 1106 14-15 0.22896493130551931 1106 16-17 0.15348665017167207 1106 18-19 0.08403309761685307 1106 20-21 0.05619160179797689 1106 22-23 0.1446684662661326 1106 24-25 -0.053927131759095914 1106 26-27 0.037816307641158176 1106 28-29 0.06671461980403848 1106 30-31 -0.04032715723111835 1106 32-33 -0.04015506709320249 1106 34-35 -0.050833820824742304 1106 36-37 -0.10133302908130304 1106 38-39 0.0859654964351364 1106 40-41 0.012842365747943063 1106 42-43 0.06688957221033576 1106 44-45 0.028724326035067804 1106 46-47 -0.07607989050531927 1106 48-49 -0.07446594527176131 1106 50-51 -0.1236202438890075 1106 52-53 -0.22336559262975442 1106 54-55 -0.33042091246390015 1106 56-57 -0.25181302635427727 1106 58-59 -0.0946676699675777 1106 60-61 -0.11376947788463099 1106 62-63 -0.05014637551905565 1106 64-65 0.12024257532110738 1106 66-67 0.031769178990593616 1106 68-69 0.04159148640827581 1106 70-71 -0.022628687872831676 1106 72-73 -0.1678934938827048 1106 74-75 -0.050422714303124394 1106 76-77 -0.3007846204078284 1106 78-79 -0.11235161960773254 1106 80-81 0.00948154646158983 1106 82-83 0.3021108345985013 1106 84-85 -0.002158000716477204 1106 86-87 0.07850701554614403 1106 88-89 0.21753718930218469 1106 90-91 0.16592347613675784 1106 92-93 0.07934546476692006 1106 94-95 0.4576443579325229 1106 96-97 0.46011322324321924 1106 98-99 0.6836675610231779 1106 100-101 0.8953443576058504 1107 1 0.10414011068211693 1107 2 0.14851758252043723 1107 3 0.1264914578871199 1107 4 0.014525145100897419 1107 5 0.0982229702109052 1107 6 0.10793759476916875 1107 7 0.04638955910169784 1107 8 0.04778870730645224 1107 9 0.0873311258343179 1107 10-11 0.06446929084879116 1107 12-13 0.06709913046700677 1107 14-15 0.28217775602356454 1107 16-17 0.19730392735566227 1107 18-19 0.11822512460879864 1107 20-21 0.0954168193842051 1107 22-23 0.016530599483928654 1107 24-25 0.09461240678001559 1107 26-27 0.1390708784360939 1107 28-29 0.0055271035786574885 1107 30-31 0.07602333048277643 1107 32-33 0.08666549813924718 1107 34-35 0.11345973164093692 1107 36-37 0.1960894944493674 1107 38-39 0.2418706932528778 1107 40-41 0.32548540383029234 1107 42-43 0.07395243055312761 1107 44-45 0.2164974994226938 1107 46-47 0.24334890078467453 1107 48-49 0.2944674236798761 1107 50-51 0.3929596585239139 1107 52-53 0.15662938802383053 1107 54-55 0.2786571713773469 1107 56-57 0.126801412528863 1107 58-59 0.15092423191726567 1107 60-61 0.16935289227467365 1107 62-63 0.2701735380400905 1107 64-65 0.20540522340954936 1107 66-67 0.21447405293987032 1107 68-69 0.29671294750324506 1107 70-71 0.11399112122365551 1107 72-73 0.10858433371936016 1107 74-75 0.2855138789029965 1107 76-77 0.017271381853316115 1107 78-79 0.14373317500663774 1107 80-81 0.4924767938094874 1107 82-83 0.37325375705425046 1107 84-85 0.364418867051878 1107 86-87 0.4788054296453268 1107 88-89 0.4354554585712904 1107 90-91 0.3644672011563159 1107 92-93 0.4508056737099544 1107 94-95 0.524925196788022 1107 96-97 0.722831485069694 1107 98-99 0.9964048502925884 1107 100-101 0.9719169680115378 1108 1 0.057490152954731144 1108 2 0.04603104767884503 1108 3 0.03867242925225867 1108 4 -0.0612035515700029 1108 5 -0.12597686306352784 1108 6 -0.004869802642993193 1108 7 0.09634287503481431 1108 8 0.12556804652566456 1108 9 0.06543247823967846 1108 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-1.1752293340240527 2316 58-59 -1.1266752671630726 2316 60-61 -0.9242515768678885 2316 62-63 -0.9586034157422461 2316 64-65 -1.1409598478801897 2316 66-67 -1.957036304617617 2316 68-69 -1.564051118033742 2316 70-71 -1.0571474600257709 2316 72-73 -0.8257881796189288 2316 74-75 -0.927345178741902 2316 76-77 -0.9459586368007216 2316 78-79 -1.2098603365184282 2316 80-81 -1.7048899537348348 2316 82-83 -1.2683701879489462 2316 84-85 -1.239076808602647 2316 86-87 -1.454085685553153 2316 88-89 -1.1373409515044983 2316 90-91 -1.25383267230929 2316 92-93 -1.2503432455297947 2316 94-95 -1.322913934951238 2316 96-97 -1.3114983066051273 2316 98-99 -1.6005382679322793 2316 100-101 -1.608235068931286 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 35.0 5 103.0 6 219.0 7 526.0 8 1227.0 9 2256.0 10 3748.0 11 5999.0 12 8174.0 13 11047.0 14 14613.0 15 18804.0 16 23664.0 17 29198.0 18 26335.0 19 19281.0 20 18595.0 21 19506.0 22 20916.0 23 23625.0 24 27975.0 25 33608.0 26 41433.0 27 51319.0 28 62830.0 29 77823.0 30 94505.0 31 115051.0 32 141730.0 33 177586.0 34 228643.0 35 301953.0 36 413934.0 37 512370.0 38 276522.0 39 12507.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.59355842433224 19.56235317007082 15.12297234014377 22.721116065453177 2 16.037317977963568 20.70323646786744 38.95079921462735 24.30864633954164 3 19.605664215835898 24.41466835702533 29.82753088855984 26.152136538578937 4 13.493906462000165 17.089269559779634 36.43967310428487 32.97715087393533 5 14.003144453198754 37.41164654358581 33.665523874420614 14.919685128794816 6 32.30476408150286 36.10815752601087 16.879135512061747 14.707942880424522 7 29.03189972718623 32.7903557297103 19.996145755735316 18.18159878736816 8 26.997987346296036 34.518022495868045 19.076273222620227 19.407716935215692 9 26.54504181889293 16.697638366482987 20.062669779452634 36.694650035171456 10-11 25.396161782357147 26.58471811026305 26.671243747475742 21.34787635990406 12-13 26.465756818141084 23.862877147380164 27.423275117531514 22.248090916947234 14-15 23.006814795255355 25.333796845173733 25.982802149119138 25.676586210451767 16-17 22.246805752901093 28.727789865469056 26.561570298457433 22.463834083172426 18-19 22.16101726495587 26.93976554412098 29.15936306306658 21.739854127856567 20-21 23.08534382804534 25.91237521174388 29.26329634418188 21.7389846160289 22-23 22.626409429201136 26.01061885343356 29.20674467548968 22.15622704187562 24-25 22.74547535260317 26.16325197181127 29.298878622455128 21.792394053130437 26-27 22.41731076883217 26.337308710307973 29.240448935734115 22.004931585125743 28-29 22.02221026531787 26.559027316873028 29.388797122014136 22.029965295794963 30-31 22.251744590991567 26.137020819747043 29.409780356770522 22.20145423249087 32-33 22.108682381834573 26.412111468381568 29.275302910926086 22.203903238857777 34-35 22.803917807047416 26.308513373915037 28.866541600568034 22.02102721846951 36-37 22.867347865963602 26.003164637834246 28.95588602760575 22.1736014685964 38-39 22.49868409237157 26.179090502855956 29.13896871884369 22.183256685928786 40-41 22.601814896976194 26.16600396326623 29.099218473143434 22.13296266661414 42-43 22.631163614985443 26.21966248878278 28.691949279090107 22.45722461714167 44-45 22.61317750566298 26.47103030281521 28.47025483183193 22.445537359689883 46-47 22.913151646573233 26.689942399216378 28.023331334045505 22.373574620164888 48-49 23.087415985071267 26.785971591969776 27.733941072963535 22.39267134999542 50-51 22.890789961913278 26.878920489904967 27.657583974722183 22.57270557345957 52-53 23.130716984828528 26.964967591108856 27.43879434595014 22.46552107811247 54-55 22.84807839869279 26.728333850322155 27.602110024212113 22.821477726772947 56-57 22.95014272668842 27.150491744282323 27.43502235749779 22.464343171531464 58-59 23.13044981213021 26.741074011150456 27.384980992978885 22.743495183740453 60-61 22.925064124427834 26.738138444879255 27.94165821671093 22.39513921398198 62-63 22.84816995867305 26.83295167543794 27.759720209034004 22.559158156855005 64-65 22.779700432610646 26.82487707323289 27.665270772525098 22.730151721631366 66-67 22.69074730203731 27.148494688189086 27.39375757074577 22.767000439027836 68-69 22.651328485805674 26.883885593615105 27.615646364132434 22.849139556446783 70-71 22.771253279747704 26.90005327094611 27.502802846748253 22.825890602557926 72-73 22.938970405301188 26.80143226601903 27.588120951167166 22.671476377512615 74-75 22.75338947874281 27.241934399063283 27.169299225857767 22.835376896336136 76-77 22.817963144197947 27.11108114079257 27.25118252131023 22.819773193699252 78-79 22.92243403549718 26.975510154828118 27.28432170063913 22.817734109035566 80-81 22.705459664046884 27.34293368389969 27.18497651848992 22.766630133563513 82-83 22.83378659556439 27.233213399999112 27.14657746347653 22.78642254095997 84-85 22.733095855531438 27.13891067218001 27.079567840799367 23.04842563148918 86-87 22.924409355199636 27.0037292674292 27.063106516147293 23.00875486122387 88-89 22.87748970735507 27.059447378270473 26.924874118188434 23.13818879618602 90-91 22.72372730142949 27.159451118709715 27.09953624539258 23.017285334468212 92-93 22.88761055555447 26.979752058856256 27.17924493313475 22.953392452454523 94-95 22.620570992334443 26.914077867947 27.13504579334885 23.330305346369705 96-97 22.793118556321428 26.954054206088756 26.96290914846864 23.28991808912118 98-99 22.69317013821849 26.998656287976015 26.68798055628116 23.620193017524333 100-101 22.409867583491895 27.11132974084923 26.820474107105145 23.65832856855373 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 485.0 1 371.0 2 337.0 3 435.0 4 387.5 5 361.0 6 640.0 7 1325.5 8 2147.5 9 2732.5 10 2666.0 11 2334.5 12 2398.0 13 2670.0 14 2992.5 15 3250.5 16 3462.5 17 3794.0 18 4038.5 19 4308.0 20 4762.5 21 5411.5 22 6287.0 23 7393.0 24 9031.0 25 11055.5 26 13893.5 27 17697.0 28 21019.5 29 24769.5 30 30067.0 31 35637.0 32 41701.5 33 46933.5 34 52583.5 35 57417.0 36 62696.5 37 68255.5 38 71187.0 39 73034.0 40 75639.5 41 83335.0 42 90721.0 43 96398.5 44 100784.5 45 103822.5 46 104860.0 47 107991.5 48 119609.5 49 119295.5 50 108236.5 51 101910.0 52 98010.5 53 96678.5 54 94503.0 55 88771.0 56 81544.5 57 73619.5 58 66689.5 59 59652.0 60 50607.0 61 41435.0 62 33668.0 63 26539.0 64 20475.5 65 16377.0 66 12572.5 67 10017.5 68 7589.0 69 5211.0 70 4069.0 71 3328.5 72 2782.5 73 2389.0 74 1975.0 75 1639.0 76 1128.0 77 664.0 78 442.5 79 312.0 80 216.5 81 152.5 82 98.0 83 72.5 84 49.5 85 26.5 86 16.5 87 9.5 88 7.0 89 3.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03776165651585546 2 0.0055719737528095 3 0.00443628483503941 4 5.678444588850445E-4 5 4.613736228440987E-4 6 1.774513934015764E-4 7 0.0 8 0.0 9 0.0016680430979748183 10-11 3.903930654834681E-4 12-13 8.695118276677244E-4 14-15 0.0018987299093968673 16-17 0.002431084089601597 18-19 0.0 20-21 0.0 22-23 0.0015260819832535572 24-25 7.985312703070939E-4 26-27 0.004276578580977991 28-29 0.005217070966006347 30-31 0.0012599048931511924 32-33 4.613736228440987E-4 34-35 0.008145018957132356 36-37 0.010256690538611116 38-39 0.008020802981751254 40-41 0.0016857882373149757 42-43 6.565701555858327E-4 44-45 0.001082453499749616 46-47 1.0647083604094585E-4 48-49 6.743152949259903E-4 50-51 8.695118276677244E-4 52-53 0.002910202851785853 54-55 0.002661770901023646 56-57 0.0017745139340157642 58-59 0.0010647083604094585 60-61 0.0036909889827527894 62-63 0.005004129293924455 64-65 0.00864188285865677 66-67 0.0029811834091464837 68-69 0.0030344188271669566 70-71 7.098055736063056E-5 72-73 0.0014196111472126111 74-75 0.0059446216789528095 76-77 0.0025020646469622273 78-79 0.0034425570319905822 80-81 0.004862168179203194 82-83 0.00480893276118272 84-85 0.005199325826666189 86-87 0.00338932161397011 88-89 0.008819334252058346 90-91 0.012155420448007983 92-93 0.0010114729423889854 94-95 0.002466574368281912 96-97 0.001330885450511823 98-99 0.0037264792614331043 100-101 0.004400794556359095 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2817673.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.12343136375461 #Duplication Level Percentage of deduplicated Percentage of total 1 74.13284167492934 36.416595598184784 2 14.262655094453914 14.012611171946238 3 4.8592700335169425 7.161120539082572 4 2.1588396131715983 4.241984382519582 5 1.1619672766630313 2.853990988104264 6 0.7223504165101058 2.129059866360824 7 0.4463383048070427 1.5347968356842343 8 0.32877490115073255 1.2920441032642571 9 0.2437970786348847 1.077853415310418 >10 1.449899869649255 13.45276896527313 >50 0.143555740860117 4.872349482469359 >100 0.08310344520107805 7.439631516605035 >500 0.005081961886124441 1.6408347803301921 >1k 0.001306790199273126 0.9723610659929299 >5k 1.4519891103034733E-4 0.5355564323497461 >10k+ 7.259945551517366E-5 0.36644085652218433 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10275 0.3646626134402395 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9395 0.33343116820156204 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5622 0.19952634674073252 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3771 0.13383384090346892 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.549027868031528E-5 2 0.0 0.0 0.0 0.0 3.549027868031528E-5 3 0.0 0.0 0.0 0.0 3.549027868031528E-5 4 0.0 0.0 0.0 0.0 3.549027868031528E-5 5 0.0 0.0 0.0 0.0 3.549027868031528E-5 6 0.0 0.0 0.0 0.0 3.549027868031528E-5 7 0.0 0.0 0.0 0.0 3.549027868031528E-5 8 0.0 0.0 0.0 0.0 3.549027868031528E-5 9 0.0 0.0 0.0 0.0 3.549027868031528E-5 10-11 0.0 0.0 0.0 0.0 3.549027868031528E-5 12-13 0.0 0.0 0.0 0.0 3.549027868031528E-5 14-15 0.0 0.0 0.0 0.0 5.323541802047292E-5 16-17 0.0 0.0 0.0 8.872569670078821E-5 7.098055736063056E-5 18-19 0.0 0.0 0.0 1.0647083604094584E-4 7.098055736063056E-5 20-21 0.0 0.0 0.0 1.0647083604094584E-4 7.098055736063056E-5 22-23 0.0 0.0 0.0 1.0647083604094584E-4 7.098055736063056E-5 24-25 0.0 0.0 0.0 1.0647083604094584E-4 1.5970625406141879E-4 26-27 0.0 0.0 0.0 1.4196111472126113E-4 1.7745139340157642E-4 28-29 0.0 0.0 0.0 5.146090408645716E-4 1.7745139340157642E-4 30-31 0.0 0.0 0.0 0.0013486305898519806 1.7745139340157642E-4 32-33 0.0 0.0 0.0 0.0025020646469622273 2.48431950762207E-4 34-35 0.0 0.0 0.0 0.004045891769555942 2.8392222944252226E-4 36-37 0.0 0.0 0.0 0.0062817793264158045 2.8392222944252226E-4 38-39 0.0 0.0 0.0 0.008908059948759137 3.016673687826799E-4 40-41 0.0 0.0 0.0 0.011339144038360734 3.194125081228375E-4 42-43 0.0 0.0 0.0 0.014302582308167059 3.194125081228375E-4 44-45 0.0 0.0 0.0 0.017478962250055276 3.194125081228375E-4 46-47 0.0 0.0 0.0 0.022589562380020675 3.3715764746299515E-4 48-49 0.0 0.0 0.0 0.029066538239178215 3.903930654834681E-4 50-51 0.0 0.0 0.0 0.035312827286913706 3.903930654834681E-4 52-53 0.0 0.0 0.0 0.04631481367781144 4.613736228440987E-4 54-55 0.0 0.0 0.0 0.06633133085350926 5.323541802047293E-4 56-57 0.0 0.0 0.0 0.09988738934574737 5.323541802047293E-4 58-59 0.0 0.0 0.0 0.1373651236321603 5.678444588850445E-4 60-61 0.0 0.0 0.0 0.17631570448380632 6.033347375653598E-4 62-63 0.0 0.0 0.0 0.22053659171947915 6.033347375653598E-4 64-65 0.0 0.0 0.0 0.2754223076985868 6.033347375653598E-4 66-67 0.0 0.0 0.0 0.3542107263688867 6.033347375653598E-4 68-69 0.0 0.0 0.0 0.45150732537097105 6.033347375653598E-4 70-71 0.0 0.0 0.0 0.5426286158826805 6.210798769055175E-4 72-73 0.0 0.0 0.0 0.6355421654677459 7.098055736063057E-4 74-75 0.0 0.0 0.0 0.738889856984824 7.098055736063057E-4 76-77 0.0 0.0 0.0 0.8611716121778503 7.098055736063057E-4 78-79 0.0 0.0 0.0 0.9743323657500356 7.098055736063057E-4 80-81 0.0 0.0 0.0 1.097323926516668 7.630409916267785E-4 82-83 0.0 0.0 0.0 1.2342099313866441 8.162764096472515E-4 84-85 0.0 0.0 0.0 1.4135245644189371 8.162764096472515E-4 86-87 3.549027868031528E-5 0.0 0.0 1.6312929144013517 8.162764096472515E-4 88-89 3.549027868031528E-5 0.0 0.0 1.8835223249823525 8.162764096472515E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12245 0.0 29.84234 1 GTATCAA 17110 0.0 28.439062 1 GTACATG 13035 0.0 25.044422 1 TACATGG 13440 0.0 23.997513 2 ATCAACG 20490 0.0 23.553068 3 TCAACGC 20615 0.0 23.47771 4 TATCAAC 20940 0.0 23.114965 2 ACATGGG 13300 0.0 23.07154 3 CAACGCA 20995 0.0 23.030151 5 AACGCAG 21430 0.0 22.584835 6 GAGTACT 11660 0.0 22.099844 12-13 GTACTTT 12490 0.0 20.460104 14-15 ACGCAGA 23835 0.0 20.266123 7 CGCAGAG 24140 0.0 20.010069 8 AGTACTT 12415 0.0 19.608084 12-13 CATGGGA 7495 0.0 18.948048 4 GCAGAGT 25415 0.0 18.91311 9 CATGGGG 6015 0.0 18.793436 4 AGAGTAC 20310 0.0 18.709753 10-11 ACTTTTT 14560 0.0 17.681463 16-17 >>END_MODULE